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The map label for this gene is murD [H]

Identifier: 183220592

GI number: 183220592

Start: 1247260

End: 1248651

Strand: Reverse

Name: murD [H]

Synonym: LEPBI_I1196

Alternate gene names: 183220592

Gene position: 1248651-1247260 (Counterclockwise)

Preceding gene: 183220596

Following gene: 183220584

Centisome position: 34.69

GC content: 39.51

Gene sequence:

>1392_bases
ATGTTTTCTGAAACACGCACTGACCTCCCTGACCTAAAAAATCGCCACTCCTTCCTCATTCTGGGGGGGGGAGCTTCCGG
TGATTCCGCAGCCAAACTCCTTACCTCACTAGGCAAAAAGGTGACACTCGCGGATCGATTTCCAGAAAAAGCAAACCTCA
GTCTTTACCATCATGTTTTGTCCGACCATGAGCCACAAATTGTTTTAGATGGAATCGATTGTATCATCAAAAGCCCTGGG
ATCCTCCCCAGCCATTCCATTTTACAAACAGCGAGGAAGAATGAAATACCTGTCCTCAGCGAAATCGCATTGGCAAGGAT
TTTTTACAAAGGCCCGATCATTGGTATCACAGGAACAGATGGAAAATCCACAACAACTGCTCTGACGTATCATCTCTTAC
AAAAGAAATATCCAAATGCAAAAATGGGAGGAAACATTGGAGTTCCATTCACTTCATTTTGTTTGGAAAATTTAGACTAT
GTTGTATTGGAATTATCAAGTTACCAGCTGGATGACTCACCTAACTTAAAGTTAACGGCATCTGCCATTTTAAATCTTGC
CTCCGACCATTTAGAAAGACACAAGACAATGGAATCCTATGCACTCGCGAAATGGAAAATCCAGAATCTAGAAGACAAAG
ACCACCAGTGTTTTGTGAATCCAATTTTTTTTTCCTACACTCCATTCAGAAAACCAAATGTTTCCAATCTACATTCCATT
GGAGACAATGAAACCTACTTTGTGAGTTTACATCCCAATTTGATCCACACACCAAAACATACTTATGACGCAACACGATT
TCCCTTAACGGGAAAACACAATTTAATGAATTTATGTTTTGCGATTGCTTTGGCAGAAGTTGCTGGCCTAGATCCAGAAC
AAATCCAAAAACAATTGGAAACATTTCAAGGTTTGCCTCATAGGTTCCAATCGATTGATCCAAAAAATTTGAGTCCACAA
TACCAATCCATTCGGTTCATCAATGATTCAAAATCGACAAACCTACATTCGATGCTCTCTGGAATTTCTGGATTCCAAAA
AGGGGATCCAATGTATTTAATATTAGGAGGCATACCAAAAGAGGAACCGATTGCTCCATTCATCAATCGATGGAAGGAAT
TAGAATGTCTATTGTATGTATTTGGAAACGCAAAAGAAATTTGGCAGAAAGATTTAGACCAGACGGGGTTGCCAGTCACA
TTCTTCGATACCTTAAATACACTTTTTAGCGAGTTAAAAATAACAATTGAAGCAAAGTTAGATGAGAAGCCAAACCTGAC
GGTATTATTTTCACCGGCAGGGGCTAGTTTTGATCTATATAAAAATTTTGAAGAGCGTGGGAACCATTTTGTTACACTTG
TTAAGGAACACTTTGGACCACCCTCTACTTAA

Upstream 100 bases:

>100_bases
AAACGACTAGTTTTCCGTCTTTCACTTCGTGTTTCAGCACCGATTTACCCAACCTGATATGATTTTACAAAATCATCGTA
TTTTAGCGGAGTGTCAATCA

Downstream 100 bases:

>100_bases
ATTTTATCCTCTGATGTGATACCCTCCATCCACATGACGAATTTCACCTGTGATCCGATTGGATGGGCGGAACAAATAAC
ATACTTCTTCCGCCAGGTCT

Product: UDP-N-acetylmuramoylalanine--D-glutamate ligase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase [H]

Number of amino acids: Translated: 463; Mature: 463

Protein sequence:

>463_residues
MFSETRTDLPDLKNRHSFLILGGGASGDSAAKLLTSLGKKVTLADRFPEKANLSLYHHVLSDHEPQIVLDGIDCIIKSPG
ILPSHSILQTARKNEIPVLSEIALARIFYKGPIIGITGTDGKSTTTALTYHLLQKKYPNAKMGGNIGVPFTSFCLENLDY
VVLELSSYQLDDSPNLKLTASAILNLASDHLERHKTMESYALAKWKIQNLEDKDHQCFVNPIFFSYTPFRKPNVSNLHSI
GDNETYFVSLHPNLIHTPKHTYDATRFPLTGKHNLMNLCFAIALAEVAGLDPEQIQKQLETFQGLPHRFQSIDPKNLSPQ
YQSIRFINDSKSTNLHSMLSGISGFQKGDPMYLILGGIPKEEPIAPFINRWKELECLLYVFGNAKEIWQKDLDQTGLPVT
FFDTLNTLFSELKITIEAKLDEKPNLTVLFSPAGASFDLYKNFEERGNHFVTLVKEHFGPPST

Sequences:

>Translated_463_residues
MFSETRTDLPDLKNRHSFLILGGGASGDSAAKLLTSLGKKVTLADRFPEKANLSLYHHVLSDHEPQIVLDGIDCIIKSPG
ILPSHSILQTARKNEIPVLSEIALARIFYKGPIIGITGTDGKSTTTALTYHLLQKKYPNAKMGGNIGVPFTSFCLENLDY
VVLELSSYQLDDSPNLKLTASAILNLASDHLERHKTMESYALAKWKIQNLEDKDHQCFVNPIFFSYTPFRKPNVSNLHSI
GDNETYFVSLHPNLIHTPKHTYDATRFPLTGKHNLMNLCFAIALAEVAGLDPEQIQKQLETFQGLPHRFQSIDPKNLSPQ
YQSIRFINDSKSTNLHSMLSGISGFQKGDPMYLILGGIPKEEPIAPFINRWKELECLLYVFGNAKEIWQKDLDQTGLPVT
FFDTLNTLFSELKITIEAKLDEKPNLTVLFSPAGASFDLYKNFEERGNHFVTLVKEHFGPPST
>Mature_463_residues
MFSETRTDLPDLKNRHSFLILGGGASGDSAAKLLTSLGKKVTLADRFPEKANLSLYHHVLSDHEPQIVLDGIDCIIKSPG
ILPSHSILQTARKNEIPVLSEIALARIFYKGPIIGITGTDGKSTTTALTYHLLQKKYPNAKMGGNIGVPFTSFCLENLDY
VVLELSSYQLDDSPNLKLTASAILNLASDHLERHKTMESYALAKWKIQNLEDKDHQCFVNPIFFSYTPFRKPNVSNLHSI
GDNETYFVSLHPNLIHTPKHTYDATRFPLTGKHNLMNLCFAIALAEVAGLDPEQIQKQLETFQGLPHRFQSIDPKNLSPQ
YQSIRFINDSKSTNLHSMLSGISGFQKGDPMYLILGGIPKEEPIAPFINRWKELECLLYVFGNAKEIWQKDLDQTGLPVT
FFDTLNTLFSELKITIEAKLDEKPNLTVLFSPAGASFDLYKNFEERGNHFVTLVKEHFGPPST

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) [H]

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786276, Length=421, Percent_Identity=31.5914489311164, Blast_Score=164, Evalue=8e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.9 [H]

Molecular weight: Translated: 51874; Mature: 51874

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFSETRTDLPDLKNRHSFLILGGGASGDSAAKLLTSLGKKVTLADRFPEKANLSLYHHVL
CCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCCEEEEHHCCCCCCCHHHHHHHH
SDHEPQIVLDGIDCIIKSPGILPSHSILQTARKNEIPVLSEIALARIFYKGPIIGITGTD
CCCCCEEEEECHHHHCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCEEEEECCC
GKSTTTALTYHLLQKKYPNAKMGGNIGVPFTSFCLENLDYVVLELSSYQLDDSPNLKLTA
CCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHCCCCEEEEEEECEECCCCCCCEEEH
SAILNLASDHLERHKTMESYALAKWKIQNLEDKDHQCFVNPIFFSYTPFRKPNVSNLHSI
HHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCEEEEEEEEEEECCCCCCCCCHHHHC
GDNETYFVSLHPNLIHTPKHTYDATRFPLTGKHNLMNLCFAIALAEVAGLDPEQIQKQLE
CCCCEEEEEECCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHH
TFQGLPHRFQSIDPKNLSPQYQSIRFINDSKSTNLHSMLSGISGFQKGDPMYLILGGIPK
HHCCCCHHHHCCCCCCCCCCHHEEEEECCCCCCCHHHHHHHHCCCCCCCCEEEEECCCCC
EEPIAPFINRWKELECLLYVFGNAKEIWQKDLDQTGLPVTFFDTLNTLFSELKITIEAKL
CCCCCHHHHHHHHHEEEEEEECCHHHHHHHHHHCCCCCEEHHHHHHHHHHHEEEEEEEEC
DEKPNLTVLFSPAGASFDLYKNFEERGNHFVTLVKEHFGPPST
CCCCCEEEEECCCCCCHHHHHHHHHCCCEEEEEEHHHCCCCCC
>Mature Secondary Structure
MFSETRTDLPDLKNRHSFLILGGGASGDSAAKLLTSLGKKVTLADRFPEKANLSLYHHVL
CCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCCEEEEHHCCCCCCCHHHHHHHH
SDHEPQIVLDGIDCIIKSPGILPSHSILQTARKNEIPVLSEIALARIFYKGPIIGITGTD
CCCCCEEEEECHHHHCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCEEEEECCC
GKSTTTALTYHLLQKKYPNAKMGGNIGVPFTSFCLENLDYVVLELSSYQLDDSPNLKLTA
CCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHCCCCEEEEEEECEECCCCCCCEEEH
SAILNLASDHLERHKTMESYALAKWKIQNLEDKDHQCFVNPIFFSYTPFRKPNVSNLHSI
HHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCEEEEEEEEEEECCCCCCCCCHHHHC
GDNETYFVSLHPNLIHTPKHTYDATRFPLTGKHNLMNLCFAIALAEVAGLDPEQIQKQLE
CCCCEEEEEECCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHH
TFQGLPHRFQSIDPKNLSPQYQSIRFINDSKSTNLHSMLSGISGFQKGDPMYLILGGIPK
HHCCCCHHHHCCCCCCCCCCHHEEEEECCCCCCCHHHHHHHHCCCCCCCCEEEEECCCCC
EEPIAPFINRWKELECLLYVFGNAKEIWQKDLDQTGLPVTFFDTLNTLFSELKITIEAKL
CCCCCHHHHHHHHHEEEEEEECCHHHHHHHHHHCCCCCEEHHHHHHHHHHHEEEEEEEEC
DEKPNLTVLFSPAGASFDLYKNFEERGNHFVTLVKEHFGPPST
CCCCCEEEEECCCCCCHHHHHHHHHCCCEEEEEEHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA