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The map label for this gene is hemK [H]

Identifier: 183220544

GI number: 183220544

Start: 1194870

End: 1195757

Strand: Reverse

Name: hemK [H]

Synonym: LEPBI_I1148

Alternate gene names: 183220544

Gene position: 1195757-1194870 (Counterclockwise)

Preceding gene: 183220545

Following gene: 183220542

Centisome position: 33.22

GC content: 40.43

Gene sequence:

>888_bases
ATGGCGGAACAACCAGGAACCCTACTTTATTATCTCAAACGATCCACAGAATTTCTCGAAAAAAAAGAAATTCCAAACCC
TCGCGTCGACGCGGAATGGATCCTTTCTGACCTCCTCAATCTCCCTCGGATCAAACTCTATTCTCAATTTGAGATGCCAC
TCTCACAAAAAGAAATTGATCTGTATAGAGAACGCATTGTCGAACGAAGCAAACGTAAACCCGTAGCCTATATAACAGGC
AAAAAAGGATTCCATCAATTTGAATACCTGGTATCGGAAGATGTCCTCATCCCAAGACCTGAAACCGAAGAACTCGTGGA
TTTTCTTTTCAAACAAAAAGAAACCCTAAGAACCGAATTTCCTGATGGGTTTCAAATCTGGGACTTATGTTCGGGAAGTG
GATGTATCGGTCTTAGTCTTTCCCAACTTCTAGAACCCAAGGCGGTGGTATTATCTGATATTTCGGAAGAAGCGATCCAA
CAAAGCAAAGCCAATGCCGAAAAATACCAACTCACTGGCATTCAATTTTATGTTTCCAATTTAGATGAATCCCTTCCCAA
TCACTTACGTTTTGATATCATTGTTTCGAATCCTCCCTATATCCCTGATTCTGAAAAAAAGGACATTATGCCAGATGTGT
TGGATTACGAACCTCACCTCGCTCTATTTGTCAGTGATATCATCGAATTCCATCGTGCCCTATTTTTATCCGCCAAACAA
AGATTAAAACCTGGTGGTTGGTTTTTAATGGAAACGCATCCAAACTACATCCATGAGTTAGAATCATTGGCTATTTCCTT
GGGATTTGTTTCGGAACGTCGGATCCTCGACAGTTCTAATAAGGAACGTTTTTTGTTTCTGAAAACTGAAGCGAAGATTG
GATCTTAA

Upstream 100 bases:

>100_bases
GAAACGTAAACCTACGTAAACGCTCCCATAGCTCAGGTGGATAGAGCACTAGTTTCCTAAACTGGGGACACAGGTTCGAA
TCCTGTTGGGGGCAAAAAAT

Downstream 100 bases:

>100_bases
ATAGAATCATATACAGTTCTTTTTCATTTTCTTCACTTAAATTCTTTTTGAAAATTTGGTCCGCATCTCGCACGACTGGT
AAGCCTTTTTCCCAAATCAA

Product: modification methylase HemK

Products: NA

Alternate protein names: HemK protein homolog; M.RcoHemKP; tRNA (guanine-N(7)-)-methyltransferase; tRNA(m7G46)-methyltransferase [H]

Number of amino acids: Translated: 295; Mature: 294

Protein sequence:

>295_residues
MAEQPGTLLYYLKRSTEFLEKKEIPNPRVDAEWILSDLLNLPRIKLYSQFEMPLSQKEIDLYRERIVERSKRKPVAYITG
KKGFHQFEYLVSEDVLIPRPETEELVDFLFKQKETLRTEFPDGFQIWDLCSGSGCIGLSLSQLLEPKAVVLSDISEEAIQ
QSKANAEKYQLTGIQFYVSNLDESLPNHLRFDIIVSNPPYIPDSEKKDIMPDVLDYEPHLALFVSDIIEFHRALFLSAKQ
RLKPGGWFLMETHPNYIHELESLAISLGFVSERRILDSSNKERFLFLKTEAKIGS

Sequences:

>Translated_295_residues
MAEQPGTLLYYLKRSTEFLEKKEIPNPRVDAEWILSDLLNLPRIKLYSQFEMPLSQKEIDLYRERIVERSKRKPVAYITG
KKGFHQFEYLVSEDVLIPRPETEELVDFLFKQKETLRTEFPDGFQIWDLCSGSGCIGLSLSQLLEPKAVVLSDISEEAIQ
QSKANAEKYQLTGIQFYVSNLDESLPNHLRFDIIVSNPPYIPDSEKKDIMPDVLDYEPHLALFVSDIIEFHRALFLSAKQ
RLKPGGWFLMETHPNYIHELESLAISLGFVSERRILDSSNKERFLFLKTEAKIGS
>Mature_294_residues
AEQPGTLLYYLKRSTEFLEKKEIPNPRVDAEWILSDLLNLPRIKLYSQFEMPLSQKEIDLYRERIVERSKRKPVAYITGK
KGFHQFEYLVSEDVLIPRPETEELVDFLFKQKETLRTEFPDGFQIWDLCSGSGCIGLSLSQLLEPKAVVLSDISEEAIQQ
SKANAEKYQLTGIQFYVSNLDESLPNHLRFDIIVSNPPYIPDSEKKDIMPDVLDYEPHLALFVSDIIEFHRALFLSAKQR
LKPGGWFLMETHPNYIHELESLAISLGFVSERRILDSSNKERFLFLKTEAKIGS

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the methyltransferase superfamily. TrmB family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=295, Percent_Identity=29.8305084745763, Blast_Score=87, Evalue=1e-17,
Organism=Escherichia coli, GI87082085, Length=243, Percent_Identity=31.2757201646091, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1787463, Length=246, Percent_Identity=28.4552845528455, Blast_Score=83, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6324266, Length=167, Percent_Identity=30.5389221556886, Blast_Score=76, Evalue=7e-15,
Organism=Drosophila melanogaster, GI24582226, Length=242, Percent_Identity=31.404958677686, Blast_Score=77, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848
- InterPro:   IPR003358 [H]

Pfam domain/function: PF02390 Methyltransf_4; PF05175 MTS [H]

EC number: =2.1.1.33 [H]

Molecular weight: Translated: 34192; Mature: 34060

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEQPGTLLYYLKRSTEFLEKKEIPNPRVDAEWILSDLLNLPRIKLYSQFEMPLSQKEID
CCCCCCCEEEEEHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCEEEHHHHCCCCCHHHHH
LYRERIVERSKRKPVAYITGKKGFHQFEYLVSEDVLIPRPETEELVDFLFKQKETLRTEF
HHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHCCEECCCCCHHHHHHHHHHHHHHHHHCC
PDGFQIWDLCSGSGCIGLSLSQLLEPKAVVLSDISEEAIQQSKANAEKYQLTGIQFYVSN
CCCCEEEEECCCCCEECCCHHHHCCCHHHHHHHHHHHHHHHHHCCCHHEEECHHHHHHHH
LDESLPNHLRFDIIVSNPPYIPDSEKKDIMPDVLDYEPHLALFVSDIIEFHRALFLSAKQ
HHHHCCCCEEEEEEECCCCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
RLKPGGWFLMETHPNYIHELESLAISLGFVSERRILDSSNKERFLFLKTEAKIGS
HCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCC
>Mature Secondary Structure 
AEQPGTLLYYLKRSTEFLEKKEIPNPRVDAEWILSDLLNLPRIKLYSQFEMPLSQKEID
CCCCCCEEEEEHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCEEEHHHHCCCCCHHHHH
LYRERIVERSKRKPVAYITGKKGFHQFEYLVSEDVLIPRPETEELVDFLFKQKETLRTEF
HHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHCCEECCCCCHHHHHHHHHHHHHHHHHCC
PDGFQIWDLCSGSGCIGLSLSQLLEPKAVVLSDISEEAIQQSKANAEKYQLTGIQFYVSN
CCCCEEEEECCCCCEECCCHHHHCCCHHHHHHHHHHHHHHHHHCCCHHEEECHHHHHHHH
LDESLPNHLRFDIIVSNPPYIPDSEKKDIMPDVLDYEPHLALFVSDIIEFHRALFLSAKQ
HHHHCCCCEEEEEEECCCCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
RLKPGGWFLMETHPNYIHELESLAISLGFVSERRILDSSNKERFLFLKTEAKIGS
HCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]