Click here to switch to the map view.

The map label for this gene is mazG [H]

Identifier: 183220067

GI number: 183220067

Start: 660028

End: 660900

Strand: Direct

Name: mazG [H]

Synonym: LEPBI_I0654

Alternate gene names: 183220067

Gene position: 660028-660900 (Clockwise)

Preceding gene: 183220065

Following gene: 183220068

Centisome position: 18.34

GC content: 42.5

Gene sequence:

>873_bases
GTGAACCCACCCAATTTTGATTCACCCATCCAAAATTTACTGCAACTTACCTCTGACCTCCGAAGTCCTGAAGGTTGTCC
ATGGGATAAGGAACAAACCCACCGATCGGTCATCCCCCATTTGCTCGAAGAAACCTATGAAGTCGTGGATACGATTGAAA
AGGGGGACGACAACCACCTAAAAGAGGAATTGGGTGATTTACTTTTCCAAATCACCTTCCATAGCCAATTGGCGAAGGAA
CGTGGAGCGTTTGACTTTCAGGATGTCGCGAGTGGTGTCTTCCAAAAACTGGTCTATCGCCACCCTCATGTGTATGGAAA
CAAAGCCGGGATTGGAACAGGCGAGGAGGTCCTCACCCAATGGGACCAATTGAAACAAAAGGAAAAAGAATCCAAAGGGA
TTTTGCCTACAAATGGTAGCCTCCTATCTGAAATTCCGAAAGCTTTGCCCGCCATCCAACGCTCTGAAAAAATACAATCC
AAAGTCACCAAACAAGGGTTTGATTGGCCAAACGTGGGGGGAGTTTTTCAAAAGTTCCAAGAAGAGATTCAGGAACTTGA
CACGGAACTCCAAAAAAAAGGATCCCTAACTTCAAAAAAATTACCCTATGACGAACGGATTGAAGATGAGTTAGGTGATC
TTTTCTTTTTACTTGTGAATTTATCTCGTAAACTTTCCATCGATCCTGAAACTTGCCTCCGTCGTGCCAATGAAAAATTT
GAATCTCGTTTTCGTTTGATGGAAGGGTATGTAGCAAAAACGGGAAAAACTTTAAAAGATCATTCCTTAGAGGAACTCGA
TACGTATTGGGATTTGGCGAAAGAAGAGTTACGAGTGAAATTTTTGAACCAAAACAATATGGATTCAAAATGA

Upstream 100 bases:

>100_bases
CACGAGATTGGAACATAGGGATAGACTCGTAAAGGGGGGGGGCTTTCGCAAGATAAAATTTGGATTTGCTTCCCGCGTGG
ATCGGGAAAAAATACAACGA

Downstream 100 bases:

>100_bases
AAAGATCGGTCTAACTTAGCTGATAACAAGAGTCACATGAATTCCTTTGATATAAACGATCTACCAACTCTAAAAGAATT
TTCGATCATTGCGTACAAGT

Product: putative nucleoside triphosphate pyrophosphohydrolase and pyrophosphatase MazG

Products: NA

Alternate protein names: NTP-PPase [H]

Number of amino acids: Translated: 290; Mature: 290

Protein sequence:

>290_residues
MNPPNFDSPIQNLLQLTSDLRSPEGCPWDKEQTHRSVIPHLLEETYEVVDTIEKGDDNHLKEELGDLLFQITFHSQLAKE
RGAFDFQDVASGVFQKLVYRHPHVYGNKAGIGTGEEVLTQWDQLKQKEKESKGILPTNGSLLSEIPKALPAIQRSEKIQS
KVTKQGFDWPNVGGVFQKFQEEIQELDTELQKKGSLTSKKLPYDERIEDELGDLFFLLVNLSRKLSIDPETCLRRANEKF
ESRFRLMEGYVAKTGKTLKDHSLEELDTYWDLAKEELRVKFLNQNNMDSK

Sequences:

>Translated_290_residues
MNPPNFDSPIQNLLQLTSDLRSPEGCPWDKEQTHRSVIPHLLEETYEVVDTIEKGDDNHLKEELGDLLFQITFHSQLAKE
RGAFDFQDVASGVFQKLVYRHPHVYGNKAGIGTGEEVLTQWDQLKQKEKESKGILPTNGSLLSEIPKALPAIQRSEKIQS
KVTKQGFDWPNVGGVFQKFQEEIQELDTELQKKGSLTSKKLPYDERIEDELGDLFFLLVNLSRKLSIDPETCLRRANEKF
ESRFRLMEGYVAKTGKTLKDHSLEELDTYWDLAKEELRVKFLNQNNMDSK
>Mature_290_residues
MNPPNFDSPIQNLLQLTSDLRSPEGCPWDKEQTHRSVIPHLLEETYEVVDTIEKGDDNHLKEELGDLLFQITFHSQLAKE
RGAFDFQDVASGVFQKLVYRHPHVYGNKAGIGTGEEVLTQWDQLKQKEKESKGILPTNGSLLSEIPKALPAIQRSEKIQS
KVTKQGFDWPNVGGVFQKFQEEIQELDTELQKKGSLTSKKLPYDERIEDELGDLFFLLVNLSRKLSIDPETCLRRANEKF
ESRFRLMEGYVAKTGKTLKDHSLEELDTYWDLAKEELRVKFLNQNNMDSK

Specific function: Involved in the regulation of bacterial cell survival under conditions of nutritional stress. Regulates the MazEF toxin- antitoxin (TA) module that mediates programmed cell death (PCD). This is achieved by lowering the cellular concentration of (p)ppGpp p

COG id: COG1694

COG function: function code R; Predicted pyrophosphatase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nucleoside triphosphate pyrophosphohydrolase family [H]

Homologues:

Organism=Escherichia coli, GI1789144, Length=268, Percent_Identity=42.910447761194, Blast_Score=219, Evalue=2e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004518
- InterPro:   IPR011551 [H]

Pfam domain/function: PF03819 MazG [H]

EC number: =3.6.1.8 [H]

Molecular weight: Translated: 33381; Mature: 33381

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPPNFDSPIQNLLQLTSDLRSPEGCPWDKEQTHRSVIPHLLEETYEVVDTIEKGDDNHL
CCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
KEELGDLLFQITFHSQLAKERGAFDFQDVASGVFQKLVYRHPHVYGNKAGIGTGEEVLTQ
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHH
WDQLKQKEKESKGILPTNGSLLSEIPKALPAIQRSEKIQSKVTKQGFDWPNVGGVFQKFQ
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
EEIQELDTELQKKGSLTSKKLPYDERIEDELGDLFFLLVNLSRKLSIDPETCLRRANEKF
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
ESRFRLMEGYVAKTGKTLKDHSLEELDTYWDLAKEELRVKFLNQNNMDSK
HHHHHHHHHHHHHCCCCHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MNPPNFDSPIQNLLQLTSDLRSPEGCPWDKEQTHRSVIPHLLEETYEVVDTIEKGDDNHL
CCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
KEELGDLLFQITFHSQLAKERGAFDFQDVASGVFQKLVYRHPHVYGNKAGIGTGEEVLTQ
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHH
WDQLKQKEKESKGILPTNGSLLSEIPKALPAIQRSEKIQSKVTKQGFDWPNVGGVFQKFQ
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
EEIQELDTELQKKGSLTSKKLPYDERIEDELGDLFFLLVNLSRKLSIDPETCLRRANEKF
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
ESRFRLMEGYVAKTGKTLKDHSLEELDTYWDLAKEELRVKFLNQNNMDSK
HHHHHHHHHHHHHCCCCHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]