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The map label for this gene is nifA [H]

Identifier: 183219984

GI number: 183219984

Start: 565407

End: 567524

Strand: Direct

Name: nifA [H]

Synonym: LEPBI_I0566

Alternate gene names: 183219984

Gene position: 565407-567524 (Clockwise)

Preceding gene: 183219979

Following gene: 183219987

Centisome position: 15.71

GC content: 37.3

Gene sequence:

>2118_bases
ATGAGCGCGAAAAAATTCAATCCCATCCAATCCATCCATGAAGTCGCTACGGCCATGAATTCCACACAGGACCCAGATGG
CCTCCTCGAATTGATTCTCGATCGATGCATCCAAATTTGTGCCGTGGAGTCAGGTTCCCTCATGCTCATCGACGAGAAAC
AAAGTGTCCTGGATGCCGTGACCTCACGTGGGATGAACCAACAATTACTTCGTGAAACCAAACTGAAAATTGGACAGGGG
ATCACGGGTATGGCAGCTTCCACGGGAAAAGCGAAGTTGGTCAATGATGTTTCGAAAGATCCAGATTACATCCAAGTAAA
AGAAGAGATCAAATCAGAGTTAGTTGCTCCAATGATAGTAGAGGATGATATCATTGGGGTGATCTCACTTGACTCGAATC
GATTGAATGCATTTACACCTGAGATGTTGGAGATTGTAAGTGTACTTGCAAACCAAGCGGGTCAGATTTTTAAAAATTTA
CAAACCATTCGTTCATTAGAACAACGAACGAAAATCCAAGCAACTCTCATCGAAATTTCAAAAGTAGTTAGCTCAACTTT
GGATCAAAATGAAGTTTTTGATTCCATTATGGTAACAATGGAAAAGTCACTTCGTTTAGAAAAAGGAAGTATTGTCTTAT
TTAATAAAGAAGAAGCCTTATTAAGGATTGTTGCCGCTTCGGGATTATCACCAGAAGAAATTGAAAAAGGAACATACCAA
CCTGGAGAAGGAATCACTGGAAAAGTATATGAGTCAGGAGAGCCAATTATCATTGAATCTGTTGCATCACATCCTGATTT
TTTAAACAGAGTTGGATACTTGTCTCATTTTAAACATGATCCACATAACGTAAGTTTATTATGTGCTCCAATTTTGAGTG
AACAAACAACCTTGGGTGTAGTAAATGCATTTATCGTTCAAAATAAACACACTGACTTAAAATCTTTTTTAGATTTTTTA
CAAGTGGTTGCATCTATCATTTCACAATCGATCAAAATTCAAAACTTAGTCGAAGAAGCAAAAAAAGAAATTTCACGAGA
AAATATCCAATTAAAAAGAGAATTAAAAAATAAATATAAATTTGGTTCACTGATAGGTAAAGCCGCGAGTATGGAAAAGA
TGTTTGAAAAAATTCAGTTAGTTGCTGATTCAAGAGCATCTGTTCTCATCACTGGAGAATCAGGAACTGGAAAGGAAATG
ATTGCAAATGCAATTCATTACAATAGTTCCCGTTCTGAAAATCCATTTGTCAAAATCAATTGCGCTGCAATTCCAGAAAA
CTTACTTGAAAGTGAACTCTTTGGTCATAAAAAAGGTTCCTTTACGGGAGCTGTCACAGACAAAAAAGGAAAGTTTGAAT
TGGCCGACACAGGCACTATCTTTTTGGATGAAATTGGAGAAATGGATTTAAACTTACAATCAAAGTTACTTCGGGTCTTA
CAAGAGCGTGAAATTGAAGCCATTGGATCAACAAAAGCAAAAAAAGTGGATGTACGGATCATTGCCGCAACCAATGCCGA
ATTAGAACAGTTAGTTGCCGAAAAGAAATTTAGAGCAGACTTGTTTTACCGGTTGAATGTAGTCAAAATCAATACTCCAC
CGCTACGTGATCGAGTTGAAGACATTCCACTCCTCATGAATCACTTCTTGGAAAAGTATACAAAAGATAATAATAAATCT
ATCAAAGGAATCTCTCGCGAAGCATCTAGACTGCTTTTGAAATATAGATGGCCAGGAAACGTTCGCGAATTGGAAAACGT
CATCGAACGAGCAGTAGTCCTTGCCCAAGATGAAATTTTAAATGAAGAAGATTTTTCAGATATTTTATCCAATATGGAAG
AAATGCCAGATAACACTGTGGAAGTATCACATTCAAATCATGTGGAATCAGTATCTGGTGCTGAACCTTTGGATTTGGGA
TCTGGTCGTTTGACTTCTGGACAGTTGGATGGTTTGGATGGGCGTGCGATGGAAATTGTTGTCAGTGAAGTTGAATCTAG
ACTCATCCAATATGCGATGAAAAAATTTCGGTATACCAAAACAAGAGTCGCTAAGTTTTTGGGAATCAATCGTAACACAT
TGGATAAAAAAATCAAAGAACTCAATATTGAATATTGA

Upstream 100 bases:

>100_bases
AACCATTTAATTGTTTGCTTGTCGTACATAATGATGAAAAAATAAGCATAGTGAAACTGTCTTCCTTTCCAGCAATCCGT
AATTCCAAAGAGCAAAACCT

Downstream 100 bases:

>100_bases
GTTCTTCTTTGTTTGATCGGTTTTGTGAACTAGTTCTTTGATTAATGAGTTGGATCTGGGAAGTGGTTTAGGTGTTGTGT
TGGTCCCGTGAGAATATCAT

Product: putative two-component sensor protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 705; Mature: 704

Protein sequence:

>705_residues
MSAKKFNPIQSIHEVATAMNSTQDPDGLLELILDRCIQICAVESGSLMLIDEKQSVLDAVTSRGMNQQLLRETKLKIGQG
ITGMAASTGKAKLVNDVSKDPDYIQVKEEIKSELVAPMIVEDDIIGVISLDSNRLNAFTPEMLEIVSVLANQAGQIFKNL
QTIRSLEQRTKIQATLIEISKVVSSTLDQNEVFDSIMVTMEKSLRLEKGSIVLFNKEEALLRIVAASGLSPEEIEKGTYQ
PGEGITGKVYESGEPIIIESVASHPDFLNRVGYLSHFKHDPHNVSLLCAPILSEQTTLGVVNAFIVQNKHTDLKSFLDFL
QVVASIISQSIKIQNLVEEAKKEISRENIQLKRELKNKYKFGSLIGKAASMEKMFEKIQLVADSRASVLITGESGTGKEM
IANAIHYNSSRSENPFVKINCAAIPENLLESELFGHKKGSFTGAVTDKKGKFELADTGTIFLDEIGEMDLNLQSKLLRVL
QEREIEAIGSTKAKKVDVRIIAATNAELEQLVAEKKFRADLFYRLNVVKINTPPLRDRVEDIPLLMNHFLEKYTKDNNKS
IKGISREASRLLLKYRWPGNVRELENVIERAVVLAQDEILNEEDFSDILSNMEEMPDNTVEVSHSNHVESVSGAEPLDLG
SGRLTSGQLDGLDGRAMEIVVSEVESRLIQYAMKKFRYTKTRVAKFLGINRNTLDKKIKELNIEY

Sequences:

>Translated_705_residues
MSAKKFNPIQSIHEVATAMNSTQDPDGLLELILDRCIQICAVESGSLMLIDEKQSVLDAVTSRGMNQQLLRETKLKIGQG
ITGMAASTGKAKLVNDVSKDPDYIQVKEEIKSELVAPMIVEDDIIGVISLDSNRLNAFTPEMLEIVSVLANQAGQIFKNL
QTIRSLEQRTKIQATLIEISKVVSSTLDQNEVFDSIMVTMEKSLRLEKGSIVLFNKEEALLRIVAASGLSPEEIEKGTYQ
PGEGITGKVYESGEPIIIESVASHPDFLNRVGYLSHFKHDPHNVSLLCAPILSEQTTLGVVNAFIVQNKHTDLKSFLDFL
QVVASIISQSIKIQNLVEEAKKEISRENIQLKRELKNKYKFGSLIGKAASMEKMFEKIQLVADSRASVLITGESGTGKEM
IANAIHYNSSRSENPFVKINCAAIPENLLESELFGHKKGSFTGAVTDKKGKFELADTGTIFLDEIGEMDLNLQSKLLRVL
QEREIEAIGSTKAKKVDVRIIAATNAELEQLVAEKKFRADLFYRLNVVKINTPPLRDRVEDIPLLMNHFLEKYTKDNNKS
IKGISREASRLLLKYRWPGNVRELENVIERAVVLAQDEILNEEDFSDILSNMEEMPDNTVEVSHSNHVESVSGAEPLDLG
SGRLTSGQLDGLDGRAMEIVVSEVESRLIQYAMKKFRYTKTRVAKFLGINRNTLDKKIKELNIEY
>Mature_704_residues
SAKKFNPIQSIHEVATAMNSTQDPDGLLELILDRCIQICAVESGSLMLIDEKQSVLDAVTSRGMNQQLLRETKLKIGQGI
TGMAASTGKAKLVNDVSKDPDYIQVKEEIKSELVAPMIVEDDIIGVISLDSNRLNAFTPEMLEIVSVLANQAGQIFKNLQ
TIRSLEQRTKIQATLIEISKVVSSTLDQNEVFDSIMVTMEKSLRLEKGSIVLFNKEEALLRIVAASGLSPEEIEKGTYQP
GEGITGKVYESGEPIIIESVASHPDFLNRVGYLSHFKHDPHNVSLLCAPILSEQTTLGVVNAFIVQNKHTDLKSFLDFLQ
VVASIISQSIKIQNLVEEAKKEISRENIQLKRELKNKYKFGSLIGKAASMEKMFEKIQLVADSRASVLITGESGTGKEMI
ANAIHYNSSRSENPFVKINCAAIPENLLESELFGHKKGSFTGAVTDKKGKFELADTGTIFLDEIGEMDLNLQSKLLRVLQ
EREIEAIGSTKAKKVDVRIIAATNAELEQLVAEKKFRADLFYRLNVVKINTPPLRDRVEDIPLLMNHFLEKYTKDNNKSI
KGISREASRLLLKYRWPGNVRELENVIERAVVLAQDEILNEEDFSDILSNMEEMPDNTVEVSHSNHVESVSGAEPLDLGS
GRLTSGQLDGLDGRAMEIVVSEVESRLIQYAMKKFRYTKTRVAKFLGINRNTLDKKIKELNIEY

Specific function: Required for activation of most nif operons, which are directly involved in nitrogen fixation [H]

COG id: COG3604

COG function: function code KT; Transcriptional regulator containing GAF, AAA-type ATPase, and DNA binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=379, Percent_Identity=44.8548812664908, Blast_Score=314, Evalue=1e-86,
Organism=Escherichia coli, GI87082117, Length=451, Percent_Identity=40.1330376940133, Blast_Score=311, Evalue=8e-86,
Organism=Escherichia coli, GI1789087, Length=501, Percent_Identity=37.9241516966068, Blast_Score=299, Evalue=4e-82,
Organism=Escherichia coli, GI1790437, Length=339, Percent_Identity=45.1327433628319, Blast_Score=288, Evalue=9e-79,
Organism=Escherichia coli, GI1790299, Length=466, Percent_Identity=37.5536480686695, Blast_Score=277, Evalue=1e-75,
Organism=Escherichia coli, GI1789233, Length=340, Percent_Identity=44.4117647058823, Blast_Score=262, Evalue=4e-71,
Organism=Escherichia coli, GI1788905, Length=240, Percent_Identity=48.75, Blast_Score=253, Evalue=4e-68,
Organism=Escherichia coli, GI87082152, Length=244, Percent_Identity=51.2295081967213, Blast_Score=252, Evalue=6e-68,
Organism=Escherichia coli, GI87081872, Length=344, Percent_Identity=37.2093023255814, Blast_Score=227, Evalue=2e-60,
Organism=Escherichia coli, GI1786524, Length=371, Percent_Identity=35.3099730458221, Blast_Score=226, Evalue=3e-60,
Organism=Escherichia coli, GI1787583, Length=252, Percent_Identity=40.4761904761905, Blast_Score=197, Evalue=2e-51,
Organism=Escherichia coli, GI1789828, Length=233, Percent_Identity=36.9098712446352, Blast_Score=145, Evalue=6e-36,
Organism=Escherichia coli, GI87081858, Length=324, Percent_Identity=29.6296296296296, Blast_Score=128, Evalue=1e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003018
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR010113
- InterPro:   IPR002078 [H]

Pfam domain/function: PF01590 GAF; PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 78581; Mature: 78450

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: PS00675 SIGMA54_INTERACT_1 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAKKFNPIQSIHEVATAMNSTQDPDGLLELILDRCIQICAVESGSLMLIDEKQSVLDAV
CCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECHHHHHHHH
TSRGMNQQLLRETKLKIGQGITGMAASTGKAKLVNDVSKDPDYIQVKEEIKSELVAPMIV
HHCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHCCCEEE
EDDIIGVISLDSNRLNAFTPEMLEIVSVLANQAGQIFKNLQTIRSLEQRTKIQATLIEIS
CCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KVVSSTLDQNEVFDSIMVTMEKSLRLEKGSIVLFNKEEALLRIVAASGLSPEEIEKGTYQ
HHHHHHCCHHHHHHHHHHHHHHHHEECCCCEEEEECHHHHHHHHHHCCCCHHHHHCCCCC
PGEGITGKVYESGEPIIIESVASHPDFLNRVGYLSHFKHDPHNVSLLCAPILSEQTTLGV
CCCCCCCCEECCCCCEEEECHHCCHHHHHHHHHHHHCCCCCCCCEEEEEEHHCCCHHHHH
VNAFIVQNKHTDLKSFLDFLQVVASIISQSIKIQNLVEEAKKEISRENIQLKRELKNKYK
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FGSLIGKAASMEKMFEKIQLVADSRASVLITGESGTGKEMIANAIHYNSSRSENPFVKIN
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHCCCCCCCCCEEEEE
CAAIPENLLESELFGHKKGSFTGAVTDKKGKFELADTGTIFLDEIGEMDLNLQSKLLRVL
EHHCCHHHHHHHHHCCCCCCEEEEEECCCCCEEECCCCEEEEHHHCCCCCCHHHHHHHHH
QEREIEAIGSTKAKKVDVRIIAATNAELEQLVAEKKFRADLFYRLNVVKINTPPLRDRVE
HHHHHHHHCCCCCEEEEEEEEEECCHHHHHHHHHHHHHHEEEEEEEEEEECCCCHHHHHH
DIPLLMNHFLEKYTKDNNKSIKGISREASRLLLKYRWPGNVRELENVIERAVVLAQDEIL
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHHHHHHHHHC
NEEDFSDILSNMEEMPDNTVEVSHSNHVESVSGAEPLDLGSGRLTSGQLDGLDGRAMEIV
CCCHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHH
VSEVESRLIQYAMKKFRYTKTRVAKFLGINRNTLDKKIKELNIEY
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCC
>Mature Secondary Structure 
SAKKFNPIQSIHEVATAMNSTQDPDGLLELILDRCIQICAVESGSLMLIDEKQSVLDAV
CCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECHHHHHHHH
TSRGMNQQLLRETKLKIGQGITGMAASTGKAKLVNDVSKDPDYIQVKEEIKSELVAPMIV
HHCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHCCCEEE
EDDIIGVISLDSNRLNAFTPEMLEIVSVLANQAGQIFKNLQTIRSLEQRTKIQATLIEIS
CCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KVVSSTLDQNEVFDSIMVTMEKSLRLEKGSIVLFNKEEALLRIVAASGLSPEEIEKGTYQ
HHHHHHCCHHHHHHHHHHHHHHHHEECCCCEEEEECHHHHHHHHHHCCCCHHHHHCCCCC
PGEGITGKVYESGEPIIIESVASHPDFLNRVGYLSHFKHDPHNVSLLCAPILSEQTTLGV
CCCCCCCCEECCCCCEEEECHHCCHHHHHHHHHHHHCCCCCCCCEEEEEEHHCCCHHHHH
VNAFIVQNKHTDLKSFLDFLQVVASIISQSIKIQNLVEEAKKEISRENIQLKRELKNKYK
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FGSLIGKAASMEKMFEKIQLVADSRASVLITGESGTGKEMIANAIHYNSSRSENPFVKIN
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHCCCCCCCCCEEEEE
CAAIPENLLESELFGHKKGSFTGAVTDKKGKFELADTGTIFLDEIGEMDLNLQSKLLRVL
EHHCCHHHHHHHHHCCCCCCEEEEEECCCCCEEECCCCEEEEHHHCCCCCCHHHHHHHHH
QEREIEAIGSTKAKKVDVRIIAATNAELEQLVAEKKFRADLFYRLNVVKINTPPLRDRVE
HHHHHHHHCCCCCEEEEEEEEEECCHHHHHHHHHHHHHHEEEEEEEEEEECCCCHHHHHH
DIPLLMNHFLEKYTKDNNKSIKGISREASRLLLKYRWPGNVRELENVIERAVVLAQDEIL
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHHHHHHHHHC
NEEDFSDILSNMEEMPDNTVEVSHSNHVESVSGAEPLDLGSGRLTSGQLDGLDGRAMEIV
CCCHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHH
VSEVESRLIQYAMKKFRYTKTRVAKFLGINRNTLDKKIKELNIEY
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1840608 [H]