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The map label for this gene is pbuE [H]

Identifier: 183219868

GI number: 183219868

Start: 444544

End: 445755

Strand: Direct

Name: pbuE [H]

Synonym: LEPBI_I0449

Alternate gene names: 183219868

Gene position: 444544-445755 (Clockwise)

Preceding gene: 183219867

Following gene: 183219871

Centisome position: 12.35

GC content: 40.76

Gene sequence:

>1212_bases
ATGGAATTTAAATTCACTCGCTATCATGTATTTGTCGTTGGTTTACTTGCTTTTTTGCAATTTACCGTTGTTTTGGATTT
TCTGATCTTATCACCGTTAGGTGTACTTGTCATGAGTGAGCTACAAATCACTACCGAAAAATTTGGATATGTGGTTTCCG
CTTATGCCTTTAGCGCCGGGATTTCTGGACTACTTGCTGCAGGGTTTGCGGATCGTTTTGATCGTAAAAAACTCCTCTTG
TTTTTTTATATCGGATTTGTTCTCGCAACTTTCCTTTGTGGGATTGCAGTTCATTATGAATTTTTACTATTTGTCAGAAT
TTTGACTGGTATGTTTGCAGGAGTATTGTCCTCTATTTCATTTGCCATTGTTGCTGATTTATTTCCTTTGCAAGTTCGGG
GCAGAGTGATGGGTTTTATCATGACTGCTTTTGCGGCAAGCCAAGTATTTGGTTTACCCATTGGAATATATATTTCGAAT
CTTTGGGGTTGGCAATCTCCATTTCTTATGATCGCAAGCGTGAGTGGGGTTGTTGGGGTTTTTATTTTTCTCTTTCTAAA
ACCTGTGACCTCACACTTGGATCAAAAAACTGACACCCATGCCTTCCATCATTTGGTAACGACACTCACCATGCCTAAAT
ACATACCGGCCTTCATTGCAACAACTCTAATGGCAACAGGTGGGTTTATGCTCATGCCATTTGGTTCCGCATTTTCTGTT
CATAACTTGGGGATGAAATTGGAAGATTTACCCTTTATTTATATGGTCACAGGGATTGTTTCTATGTTAGGTGGTCCAAT
GATGGGACGATTGAGTGATTCGATAGGGAAATACAATATGTTTGTTATCGCATCGTCCATTGCAGCACTCATCATCCTTT
ATTTTACCAGGCTAACCGTGACACCTCTTCCGATTGTCATTGTTCTCAATTCACTTTTATTTGTGTTCATAGCAGCTCGT
ATGATTTCAGCCAATGCGATTAATTCTGCAGTGCCAGAACTGCATGACCGTGGTGCCTTTATGGCCATCAGCTCTTCTAT
CCAACAAATCTCAGGTGGGATTGCGGCTTCCGTTGCCGGGCTCATTGTGATCCAAACTCCCAGCGGTTATTTAGAGCATT
ACGAAGTGTTAGGGTATGTGGTGGCCACTGCAATTGTATGTACAGTGATCCTGATGTATCAAGTGAATCAGATGGTAATG
GGAAAAAAATAA

Upstream 100 bases:

>100_bases
TCGGCGAATAAAATAGATTGAGAAACGATACTTTCGGTTGAAGGGAGAGTTCGCCGAACTCCGGGATTCAAGTCTATCGC
TTGAAAGAATAGGAAATAGA

Downstream 100 bases:

>100_bases
GGTAATGGTGAAACCTATTGTTTATCTAAATATAGGTGAAACATTTTTTTCTAAAGGATTCCAAATTGTTGGATAGAGAG
TTTTTGTATAAAAAATACCT

Product: major facilitator superfamily permease

Products: Proton [Cytoplasm]; N-acetylneuraminate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 403; Mature: 403

Protein sequence:

>403_residues
MEFKFTRYHVFVVGLLAFLQFTVVLDFLILSPLGVLVMSELQITTEKFGYVVSAYAFSAGISGLLAAGFADRFDRKKLLL
FFYIGFVLATFLCGIAVHYEFLLFVRILTGMFAGVLSSISFAIVADLFPLQVRGRVMGFIMTAFAASQVFGLPIGIYISN
LWGWQSPFLMIASVSGVVGVFIFLFLKPVTSHLDQKTDTHAFHHLVTTLTMPKYIPAFIATTLMATGGFMLMPFGSAFSV
HNLGMKLEDLPFIYMVTGIVSMLGGPMMGRLSDSIGKYNMFVIASSIAALIILYFTRLTVTPLPIVIVLNSLLFVFIAAR
MISANAINSAVPELHDRGAFMAISSSIQQISGGIAASVAGLIVIQTPSGYLEHYEVLGYVVATAIVCTVILMYQVNQMVM
GKK

Sequences:

>Translated_403_residues
MEFKFTRYHVFVVGLLAFLQFTVVLDFLILSPLGVLVMSELQITTEKFGYVVSAYAFSAGISGLLAAGFADRFDRKKLLL
FFYIGFVLATFLCGIAVHYEFLLFVRILTGMFAGVLSSISFAIVADLFPLQVRGRVMGFIMTAFAASQVFGLPIGIYISN
LWGWQSPFLMIASVSGVVGVFIFLFLKPVTSHLDQKTDTHAFHHLVTTLTMPKYIPAFIATTLMATGGFMLMPFGSAFSV
HNLGMKLEDLPFIYMVTGIVSMLGGPMMGRLSDSIGKYNMFVIASSIAALIILYFTRLTVTPLPIVIVLNSLLFVFIAAR
MISANAINSAVPELHDRGAFMAISSSIQQISGGIAASVAGLIVIQTPSGYLEHYEVLGYVVATAIVCTVILMYQVNQMVM
GKK
>Mature_403_residues
MEFKFTRYHVFVVGLLAFLQFTVVLDFLILSPLGVLVMSELQITTEKFGYVVSAYAFSAGISGLLAAGFADRFDRKKLLL
FFYIGFVLATFLCGIAVHYEFLLFVRILTGMFAGVLSSISFAIVADLFPLQVRGRVMGFIMTAFAASQVFGLPIGIYISN
LWGWQSPFLMIASVSGVVGVFIFLFLKPVTSHLDQKTDTHAFHHLVTTLTMPKYIPAFIATTLMATGGFMLMPFGSAFSV
HNLGMKLEDLPFIYMVTGIVSMLGGPMMGRLSDSIGKYNMFVIASSIAALIILYFTRLTVTPLPIVIVLNSLLFVFIAAR
MISANAINSAVPELHDRGAFMAISSSIQQISGGIAASVAGLIVIQTPSGYLEHYEVLGYVVATAIVCTVILMYQVNQMVM
GKK

Specific function: Involved in the efflux of purine ribonucleosides, such as guanosine, adenosine and inosine, as well as purine bases guanine, adenine and hypoxanthine, and purine base analogs 2,6- diaminopurine and 6-mercaptopurine. Therefore plays a role in maintaining t

COG id: COG2814

COG function: function code G; Arabinose efflux permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. DHA1 family. PbuE (TC 2.A.1.2.25) subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 43987; Mature: 43987

Theoretical pI: Translated: 9.14; Mature: 9.14

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
5.2 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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EEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure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EEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; N-acetylneuraminate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + N-acetylneuraminate [Periplasm] = Proton [Cytoplasm] + N-acetylneuraminate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA