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The map label for this gene is yngF [H]

Identifier: 183219761

GI number: 183219761

Start: 339504

End: 340241

Strand: Direct

Name: yngF [H]

Synonym: LEPBI_I0338

Alternate gene names: 183219761

Gene position: 339504-340241 (Clockwise)

Preceding gene: 183219760

Following gene: 183219762

Centisome position: 9.43

GC content: 42.28

Gene sequence:

>738_bases
TTGGAAATCAAGATGCAGTCCAATGAAAAAAACACATTTGATTTTGAAGCCTTTGTTTTATTCCAACAAATTTTAAACAA
ACACGCAAATAATCCTAATTTGCGTGTTTTGCTTTTTACCTCCGCACAAACTCAGTTTTTTTCCAATGGGATTGAACCCA
CTCTCATGTATGGAAAAACCGAATCGGATGTGAGACGTTCCGTGGAACAGTTGTTACGTACGGCGCAAACCTATTTCCAC
TTTCCCGTACCTACGATTGCTGTGATCAATGGCCATTGTATGGCCGCAGGGGCAGTGTTTGCTCTCTTTTCGGATTACCG
TTATATGGTGGACAAAGGAGGCAGGATTGGTTTTTCAGAAGCCATTGTGGGTCTCAACTTTCCTTCCATTCCTACAATCG
TATTGCAAGACTTAGTTGGTGTAAAAGCCGCGCGAGATCTCTTGTATACGGGGAAACAAATCAAAGGCCCTGAAGCAAAA
GAAATTGGCCTTGTGGATGAACTCTATACAGCAGAAGATTTGTATCCGGAAGCGATGAAATTTGCAGAAACATTATCTAA
ACTTACTTACAATTCAAGCCGTGGGATGAAGACCGCCTTACGTGAACCATACCGCGCCCAAATGGAATCCTTATTCGCAT
TGGATGCAGACCTCTTTACTAAGGTGATTTTATCACCTGATGGCCAAGAAGGGTTTTTGTCCCTGATCGAAAAACGTAGG
CCAAAGTTTTCGACATGA

Upstream 100 bases:

>100_bases
CGCCATGTGATCACAGAAAAATACAAAGACAGAATTTTGGAGCTCTACAAAAACAGTTAATATGACTCCTTTTGCAGAGA
TATTTCATGGAGACCGCATC

Downstream 100 bases:

>100_bases
TGCGAATCCTGATTGTATTTGTGGTCATGATTTTTTTAAATTGTGCCACAAACGCAGTCAGACAGAGGTCAAAACTTGTT
TCTACTAAAATCCATCATTT

Product: enoyl-CoA hydratase

Products: trans-2-enoyl-CoA; trans-3-enoyl-CoA; H2O

Alternate protein names: NA

Number of amino acids: Translated: 245; Mature: 245

Protein sequence:

>245_residues
MEIKMQSNEKNTFDFEAFVLFQQILNKHANNPNLRVLLFTSAQTQFFSNGIEPTLMYGKTESDVRRSVEQLLRTAQTYFH
FPVPTIAVINGHCMAAGAVFALFSDYRYMVDKGGRIGFSEAIVGLNFPSIPTIVLQDLVGVKAARDLLYTGKQIKGPEAK
EIGLVDELYTAEDLYPEAMKFAETLSKLTYNSSRGMKTALREPYRAQMESLFALDADLFTKVILSPDGQEGFLSLIEKRR
PKFST

Sequences:

>Translated_245_residues
MEIKMQSNEKNTFDFEAFVLFQQILNKHANNPNLRVLLFTSAQTQFFSNGIEPTLMYGKTESDVRRSVEQLLRTAQTYFH
FPVPTIAVINGHCMAAGAVFALFSDYRYMVDKGGRIGFSEAIVGLNFPSIPTIVLQDLVGVKAARDLLYTGKQIKGPEAK
EIGLVDELYTAEDLYPEAMKFAETLSKLTYNSSRGMKTALREPYRAQMESLFALDADLFTKVILSPDGQEGFLSLIEKRR
PKFST
>Mature_245_residues
MEIKMQSNEKNTFDFEAFVLFQQILNKHANNPNLRVLLFTSAQTQFFSNGIEPTLMYGKTESDVRRSVEQLLRTAQTYFH
FPVPTIAVINGHCMAAGAVFALFSDYRYMVDKGGRIGFSEAIVGLNFPSIPTIVLQDLVGVKAARDLLYTGKQIKGPEAK
EIGLVDELYTAEDLYPEAMKFAETLSKLTYNSSRGMKTALREPYRAQMESLFALDADLFTKVILSPDGQEGFLSLIEKRR
PKFST

Specific function: Could Possibly Oxidizes Fatty Acids Using Specific Components (By Similarity). [C]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI31542718, Length=241, Percent_Identity=25.7261410788382, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI20127408, Length=209, Percent_Identity=25.3588516746411, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI1787659, Length=232, Percent_Identity=25, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI1788682, Length=188, Percent_Identity=28.1914893617021, Blast_Score=65, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17554946, Length=166, Percent_Identity=26.5060240963855, Blast_Score=65, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI25145438, Length=170, Percent_Identity=26.4705882352941, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24653139, Length=238, Percent_Identity=23.5294117647059, Blast_Score=71, Evalue=8e-13,
Organism=Drosophila melanogaster, GI20129971, Length=220, Percent_Identity=24.0909090909091, Blast_Score=65, Evalue=6e-11,
Organism=Drosophila melanogaster, GI24653477, Length=220, Percent_Identity=24.0909090909091, Blast_Score=65, Evalue=6e-11,
Organism=Drosophila melanogaster, GI24583165, Length=199, Percent_Identity=27.1356783919598, Blast_Score=64, Evalue=7e-11,
Organism=Drosophila melanogaster, GI19921018, Length=179, Percent_Identity=27.3743016759777, Blast_Score=64, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: 4.2.1.17

Molecular weight: Translated: 27579; Mature: 27579

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIKMQSNEKNTFDFEAFVLFQQILNKHANNPNLRVLLFTSAQTQFFSNGIEPTLMYGKT
CCEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCHHHHHHCCCCCEEEECCC
ESDVRRSVEQLLRTAQTYFHFPVPTIAVINGHCMAAGAVFALFSDYRYMVDKGGRIGFSE
HHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCHHH
AIVGLNFPSIPTIVLQDLVGVKAARDLLYTGKQIKGPEAKEIGLVDELYTAEDLYPEAMK
HHEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCHHHHHHHHHCCHHHHH
FAETLSKLTYNSSRGMKTALREPYRAQMESLFALDADLFTKVILSPDGQEGFLSLIEKRR
HHHHHHHHHCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCC
PKFST
CCCCC
>Mature Secondary Structure
MEIKMQSNEKNTFDFEAFVLFQQILNKHANNPNLRVLLFTSAQTQFFSNGIEPTLMYGKT
CCEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCHHHHHHCCCCCEEEECCC
ESDVRRSVEQLLRTAQTYFHFPVPTIAVINGHCMAAGAVFALFSDYRYMVDKGGRIGFSE
HHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCHHH
AIVGLNFPSIPTIVLQDLVGVKAARDLLYTGKQIKGPEAKEIGLVDELYTAEDLYPEAMK
HHEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCHHHHHHHHHCCHHHHH
FAETLSKLTYNSSRGMKTALREPYRAQMESLFALDADLFTKVILSPDGQEGFLSLIEKRR
HHHHHHHHHCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCC
PKFST
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.05 {crotonyl-CoA}} 0.008 {2-decenoyl-CoA}} [C]

Substrates: (3S)-3-hydroxyacyl-CoA

Specific reaction: (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O

General reaction: addition (of H2O to carbon carbon double bondelimination (of H2O C-O bond cleavagehydration [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9387222; 9384377 [H]