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The map label for this gene is panB

Identifier: 183219695

GI number: 183219695

Start: 264498

End: 265304

Strand: Direct

Name: panB

Synonym: LEPBI_I0271

Alternate gene names: 183219695

Gene position: 264498-265304 (Clockwise)

Preceding gene: 183219694

Following gene: 183219700

Centisome position: 7.35

GC content: 44.98

Gene sequence:

>807_bases
ATGAAAAACATTATTCTACAGTATAAAAAAAAATACGACGCGGGCGAACCCATCTCTGTTGTCACTTGTTATGATTACAC
GTTTGCGACACTTTTTAATCGCACAGATGTAGATTGCCTTCTTGTGGGAGATTCCCTGGGAATGGTGATCCAAGGAAATC
AGTCCACACTCCCCGTGACATTAGATGAGATCATCTACCACACAAAAGCGGTTTGTAAAGGGGCTCCCGACAAAACCATC
ATTGCGGATTTACCATTTTTATCGTATCAAACCTCCATTGAAGAAGGGATCCGTTCGGCAGGACGTGTCTTAAAAGAAAC
AAACGCATCTTGTGTGAAACTCGAAGGGGATTCTGAATTCATCATCGAACTCACCAAACGCATGACAGAATCGGGAATCC
CTGTCTTTGCCCACTTGGGTCTTACCCCACAATCGGTGCACACACTTGGTGGCCACCGTGTCCAAGGCAAAACCGAAGCG
GCTCGAAACAAAATGATCCGTAAGTCGAGAGAACTAGCAGAAGCAGGTGCCTTTGCTTTGTTACTAGAGATGGTGCCTGA
ATCTTTAGGAAAAGAGATCACAGAATCCATTCGGATTCCTACCATCGGCATTGGTGCAGGTAAGTACACATCAGGCCAAG
TCCTTGTGATGCAAGACCTTCTAGGGCTCAATGAAGACTTTCATCCTAAGTTTTTGAAGAAGTTTGGAAATTTAAGTGGA
GCCGTGAAAGAGGCAGTGAATGCCTACCACAAAGAAGTCACAAAACGGGAATACCCATCCGAGGCCCATGTGTTCTTAGA
TACTTGA

Upstream 100 bases:

>100_bases
CATTTACCCCACCATTCCCTGTATTCCAGACCATTCATTAAGCAGTTGTTAACTGACATGGGTGAGATTGGTGTGTATGC
CCTCTATACGGAACTGAACC

Downstream 100 bases:

>100_bases
TTTCCTTGGAAATGGAAATGGAAATTAGGAAACGGAAAGACGTTCCATTAGAAAAATCAACTCGTCGTCAGAGTGAAATG
AGAGGGTTTTGACACCCTCT

Product: 3-methyl-2-oxobutanoate hydroxymethyltransferase

Products: NA

Alternate protein names: Ketopantoate hydroxymethyltransferase; KPHMT

Number of amino acids: Translated: 268; Mature: 268

Protein sequence:

>268_residues
MKNIILQYKKKYDAGEPISVVTCYDYTFATLFNRTDVDCLLVGDSLGMVIQGNQSTLPVTLDEIIYHTKAVCKGAPDKTI
IADLPFLSYQTSIEEGIRSAGRVLKETNASCVKLEGDSEFIIELTKRMTESGIPVFAHLGLTPQSVHTLGGHRVQGKTEA
ARNKMIRKSRELAEAGAFALLLEMVPESLGKEITESIRIPTIGIGAGKYTSGQVLVMQDLLGLNEDFHPKFLKKFGNLSG
AVKEAVNAYHKEVTKREYPSEAHVFLDT

Sequences:

>Translated_268_residues
MKNIILQYKKKYDAGEPISVVTCYDYTFATLFNRTDVDCLLVGDSLGMVIQGNQSTLPVTLDEIIYHTKAVCKGAPDKTI
IADLPFLSYQTSIEEGIRSAGRVLKETNASCVKLEGDSEFIIELTKRMTESGIPVFAHLGLTPQSVHTLGGHRVQGKTEA
ARNKMIRKSRELAEAGAFALLLEMVPESLGKEITESIRIPTIGIGAGKYTSGQVLVMQDLLGLNEDFHPKFLKKFGNLSG
AVKEAVNAYHKEVTKREYPSEAHVFLDT
>Mature_268_residues
MKNIILQYKKKYDAGEPISVVTCYDYTFATLFNRTDVDCLLVGDSLGMVIQGNQSTLPVTLDEIIYHTKAVCKGAPDKTI
IADLPFLSYQTSIEEGIRSAGRVLKETNASCVKLEGDSEFIIELTKRMTESGIPVFAHLGLTPQSVHTLGGHRVQGKTEA
ARNKMIRKSRELAEAGAFALLLEMVPESLGKEITESIRIPTIGIGAGKYTSGQVLVMQDLLGLNEDFHPKFLKKFGNLSG
AVKEAVNAYHKEVTKREYPSEAHVFLDT

Specific function: Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is tranferred onto alpha-ketoisovalerate to form ketopantoate

COG id: COG0413

COG function: function code H; Ketopantoate hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the panB family

Homologues:

Organism=Escherichia coli, GI1786326, Length=259, Percent_Identity=45.5598455598456, Blast_Score=227, Evalue=7e-61,
Organism=Saccharomyces cerevisiae, GI6319653, Length=270, Percent_Identity=37.4074074074074, Blast_Score=201, Evalue=9e-53,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PANB_LEPBA (B0SAC5)

Other databases:

- EMBL:   CP000777
- RefSeq:   YP_001961385.1
- ProteinModelPortal:   B0SAC5
- SMR:   B0SAC5
- GeneID:   6387091
- GenomeReviews:   CP000777_GR
- KEGG:   lbf:LBF_0262
- HOGENOM:   HBG299908
- OMA:   YATPEQT
- ProtClustDB:   PRK00311
- BioCyc:   LBIF355278:LBF_0262-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00156
- InterPro:   IPR003700
- InterPro:   IPR015813
- Gene3D:   G3DSA:3.20.20.60
- PANTHER:   PTHR20881
- PIRSF:   PIRSF000388
- TIGRFAMs:   TIGR00222

Pfam domain/function: PF02548 Pantoate_transf; SSF51621 Pyrv/PenolPyrv_Kinase_cat

EC number: =2.1.2.11

Molecular weight: Translated: 29483; Mature: 29483

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: NA

Important sites: ACT_SITE 183-183 BINDING 83-83 BINDING 113-113

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNIILQYKKKYDAGEPISVVTCYDYTFATLFNRTDVDCLLVGDSLGMVIQGNQSTLPVT
CCCHHHHHHHHCCCCCCEEEEEECCHHHHHHHCCCCCEEEEEECCCCEEEECCCCCCCEE
LDEIIYHTKAVCKGAPDKTIIADLPFLSYQTSIEEGIRSAGRVLKETNASCVKLEGDSEF
HHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHH
IIELTKRMTESGIPVFAHLGLTPQSVHTLGGHRVQGKTEAARNKMIRKSRELAEAGAFAL
HHHHHHHHHHCCCCEEEECCCCHHHHHHCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHH
LLEMVPESLGKEITESIRIPTIGIGAGKYTSGQVLVMQDLLGLNEDFHPKFLKKFGNLSG
HHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCEEHHHHHHCCCCCCCHHHHHHHCCCCH
AVKEAVNAYHKEVTKREYPSEAHVFLDT
HHHHHHHHHHHHHHHHCCCCCCEEEEEC
>Mature Secondary Structure
MKNIILQYKKKYDAGEPISVVTCYDYTFATLFNRTDVDCLLVGDSLGMVIQGNQSTLPVT
CCCHHHHHHHHCCCCCCEEEEEECCHHHHHHHCCCCCEEEEEECCCCEEEECCCCCCCEE
LDEIIYHTKAVCKGAPDKTIIADLPFLSYQTSIEEGIRSAGRVLKETNASCVKLEGDSEF
HHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHH
IIELTKRMTESGIPVFAHLGLTPQSVHTLGGHRVQGKTEAARNKMIRKSRELAEAGAFAL
HHHHHHHHHHCCCCEEEECCCCHHHHHHCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHH
LLEMVPESLGKEITESIRIPTIGIGAGKYTSGQVLVMQDLLGLNEDFHPKFLKKFGNLSG
HHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCEEHHHHHHCCCCCCCHHHHHHHCCCCH
AVKEAVNAYHKEVTKREYPSEAHVFLDT
HHHHHHHHHHHHHHHHCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA