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The map label for this gene is rlmI [H]

Identifier: 18311530

GI number: 18311530

Start: 2918053

End: 2919228

Strand: Direct

Name: rlmI [H]

Synonym: CPE2548

Alternate gene names: 18311530

Gene position: 2918053-2919228 (Clockwise)

Preceding gene: 18311524

Following gene: 18311541

Centisome position: 96.26

GC content: 31.46

Gene sequence:

>1176_bases
ATGGCGAGTAAGTTTTATTTACACAAAGGTAAAAACAAAAAAGCTGAACAAGGCAGACCTTGGATATATATTGATGAAAT
AAACGAATATGATGGAGATTATGAAAACGGAGATATAGTTGAAGTTTACAATCATAAAGGTTATTTCTTAGGAAAAGGCT
ATATAAATGACAGAAGTAAGATAACTATAAGAATAATGACTAAAGATATAGATGAAGAAATAGATGAGGATTTCTTCAAG
AGAAGATTTAAAACTGCATGGGAATATAGAAAGAAAGTTATAGATACATCTTCATGTAGATTCATCTTTGGAGAGGCTGA
TTTCCTTCCTGGTTTAACAGTTGATAAATTTGAAGATTATTATGTAATTCAAATATCAACTCTTGGAATGGATAAATATA
GAGACCTAATAGTTAAAATTCTAGTTGAGGAATACGGTGCTAAAGGTGTCTATGAAAGAAGTGATATAAAAACTAGAGAA
ATAGAAGGTTTAGAGCAAAGAAAAGGCTTCTTAACTGAACCATTTGATACAGATATACAAATAGTTGAAAATGGAGTTAA
ATACATAGTTGACTTAGAAAATGGTCAAAAAACTGGTTTCTTCTTAGATCAAAAAGAAAACAGAGCTGCAATGCATAGAA
TATGTAAAGATATGGATGTTTTAGATTGCTTCACTCATACTGGCTCTTTTGCTTTAAATGCCGGTATAGCAGGTGCTAAA
TCAGTTTTAGGAATAGATGTATCTCAACATGCTGTAGACTGTGCTACTAGAAATGCTGAGCTTAATAACCTTCAAGATAG
GGTTAAATTTGAAAAGCATAATGCCTTTGACGTATTAGGAGATTGGTCAAGAGAAGGAAAACAATTTGATGTTGTTATTT
TAGATCCACCAGCTTTCACAAAATCAAGAAATACTGTTAAGCAAGCAATAAGAGGATATAAAGAAATAAATCTTAGAGGA
ATAAAAATGGTTAAAGAAGGTGGTTACTTCGCTACATGCTCTTGTTCACATTATATGGATGAAGAACAATTAAAGAAAAC
TGTAGCTGAGGCTGCTCATGATGCAAGAAGAACTTTAAGACAAATAGAAGTTAGAACTCAAAGTGCAGACCACCCTATAC
TTTGGAACTCTGACGAATCATATTATTTAAAATTCTTCATATTCCAAGTATTCTAA

Upstream 100 bases:

>100_bases
TAAAGTACCAATTTATTTTTCTTAAATGCATTTTAAACCTATTGGAAAGCCTAGAATATGTTATAATCTTATAAGAATTA
TTTTGGAAGGAGCTATTTAA

Downstream 100 bases:

>100_bases
AAAAATTTTAAATCTTAAGTATAAAAAAAAATATATTGGTTAATATATTAATGTAGCAAAAATATATAAAGTTAATATCT
TAACTTATAAATAAGAGGTG

Product: putative methyltransferase

Products: NA

Alternate protein names: 23S rRNA m5C1962 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase RlmI [H]

Number of amino acids: Translated: 391; Mature: 390

Protein sequence:

>391_residues
MASKFYLHKGKNKKAEQGRPWIYIDEINEYDGDYENGDIVEVYNHKGYFLGKGYINDRSKITIRIMTKDIDEEIDEDFFK
RRFKTAWEYRKKVIDTSSCRFIFGEADFLPGLTVDKFEDYYVIQISTLGMDKYRDLIVKILVEEYGAKGVYERSDIKTRE
IEGLEQRKGFLTEPFDTDIQIVENGVKYIVDLENGQKTGFFLDQKENRAAMHRICKDMDVLDCFTHTGSFALNAGIAGAK
SVLGIDVSQHAVDCATRNAELNNLQDRVKFEKHNAFDVLGDWSREGKQFDVVILDPPAFTKSRNTVKQAIRGYKEINLRG
IKMVKEGGYFATCSCSHYMDEEQLKKTVAEAAHDARRTLRQIEVRTQSADHPILWNSDESYYLKFFIFQVF

Sequences:

>Translated_391_residues
MASKFYLHKGKNKKAEQGRPWIYIDEINEYDGDYENGDIVEVYNHKGYFLGKGYINDRSKITIRIMTKDIDEEIDEDFFK
RRFKTAWEYRKKVIDTSSCRFIFGEADFLPGLTVDKFEDYYVIQISTLGMDKYRDLIVKILVEEYGAKGVYERSDIKTRE
IEGLEQRKGFLTEPFDTDIQIVENGVKYIVDLENGQKTGFFLDQKENRAAMHRICKDMDVLDCFTHTGSFALNAGIAGAK
SVLGIDVSQHAVDCATRNAELNNLQDRVKFEKHNAFDVLGDWSREGKQFDVVILDPPAFTKSRNTVKQAIRGYKEINLRG
IKMVKEGGYFATCSCSHYMDEEQLKKTVAEAAHDARRTLRQIEVRTQSADHPILWNSDESYYLKFFIFQVF
>Mature_390_residues
ASKFYLHKGKNKKAEQGRPWIYIDEINEYDGDYENGDIVEVYNHKGYFLGKGYINDRSKITIRIMTKDIDEEIDEDFFKR
RFKTAWEYRKKVIDTSSCRFIFGEADFLPGLTVDKFEDYYVIQISTLGMDKYRDLIVKILVEEYGAKGVYERSDIKTREI
EGLEQRKGFLTEPFDTDIQIVENGVKYIVDLENGQKTGFFLDQKENRAAMHRICKDMDVLDCFTHTGSFALNAGIAGAKS
VLGIDVSQHAVDCATRNAELNNLQDRVKFEKHNAFDVLGDWSREGKQFDVVILDPPAFTKSRNTVKQAIRGYKEINLRGI
KMVKEGGYFATCSCSHYMDEEQLKKTVAEAAHDARRTLRQIEVRTQSADHPILWNSDESYYLKFFIFQVF

Specific function: Specifically methylates the cytosine at position 1962 (m5C1962) of 23S rRNA [H]

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Escherichia coli, GI145693111, Length=398, Percent_Identity=35.929648241206, Blast_Score=277, Evalue=7e-76,
Organism=Escherichia coli, GI1787181, Length=198, Percent_Identity=29.7979797979798, Blast_Score=84, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002478
- InterPro:   IPR015947
- InterPro:   IPR019614 [H]

Pfam domain/function: PF10672 Methyltrans_SAM [H]

EC number: =2.1.1.191 [H]

Molecular weight: Translated: 45343; Mature: 45212

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASKFYLHKGKNKKAEQGRPWIYIDEINEYDGDYENGDIVEVYNHKGYFLGKGYINDRSK
CCCCEEEECCCCCCCCCCCCEEEEECCCCCCCCCCCCCEEEEECCCCEEEECCEECCCCE
ITIRIMTKDIDEEIDEDFFKRRFKTAWEYRKKVIDTSSCRFIFGEADFLPGLTVDKFEDY
EEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCE
YVIQISTLGMDKYRDLIVKILVEEYGAKGVYERSDIKTREIEGLEQRKGFLTEPFDTDIQ
EEEEEEECCCHHHHHHHHHHHHHHHCCCCCEECCCCCHHHHCCHHHHCCCCCCCCCCCHH
IVENGVKYIVDLENGQKTGFFLDQKENRAAMHRICKDMDVLDCFTHTGSFALNAGIAGAK
HEECCCEEEEECCCCCCCCEEEECCCCHHHHHHHHHCCHHHHHHHCCCCEEEECCCCCCC
SVLGIDVSQHAVDCATRNAELNNLQDRVKFEKHNAFDVLGDWSREGKQFDVVILDPPAFT
CEEECCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHCCCCCCCCCEEEEEEECCCCCC
KSRNTVKQAIRGYKEINLRGIKMVKEGGYFATCSCSHYMDEEQLKKTVAEAAHDARRTLR
CCHHHHHHHHCCHHHCCCEEEEEEECCCEEEEECHHHCCCHHHHHHHHHHHHHHHHHHHH
QIEVRTQSADHPILWNSDESYYLKFFIFQVF
HHHHHCCCCCCCEEECCCCCEEEEEEEEEEC
>Mature Secondary Structure 
ASKFYLHKGKNKKAEQGRPWIYIDEINEYDGDYENGDIVEVYNHKGYFLGKGYINDRSK
CCCEEEECCCCCCCCCCCCEEEEECCCCCCCCCCCCCEEEEECCCCEEEECCEECCCCE
ITIRIMTKDIDEEIDEDFFKRRFKTAWEYRKKVIDTSSCRFIFGEADFLPGLTVDKFEDY
EEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCE
YVIQISTLGMDKYRDLIVKILVEEYGAKGVYERSDIKTREIEGLEQRKGFLTEPFDTDIQ
EEEEEEECCCHHHHHHHHHHHHHHHCCCCCEECCCCCHHHHCCHHHHCCCCCCCCCCCHH
IVENGVKYIVDLENGQKTGFFLDQKENRAAMHRICKDMDVLDCFTHTGSFALNAGIAGAK
HEECCCEEEEECCCCCCCCEEEECCCCHHHHHHHHHCCHHHHHHHCCCCEEEECCCCCCC
SVLGIDVSQHAVDCATRNAELNNLQDRVKFEKHNAFDVLGDWSREGKQFDVVILDPPAFT
CEEECCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHCCCCCCCCCEEEEEEECCCCCC
KSRNTVKQAIRGYKEINLRGIKMVKEGGYFATCSCSHYMDEEQLKKTVAEAAHDARRTLR
CCHHHHHHHHCCHHHCCCEEEEEEECCCEEEEECHHHCCCHHHHHHHHHHHHHHHHHHHH
QIEVRTQSADHPILWNSDESYYLKFFIFQVF
HHHHHCCCCCCCEEECCCCCEEEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA