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The map label for this gene is gadC [H]

Identifier: 18311042

GI number: 18311042

Start: 2354027

End: 2355445

Strand: Reverse

Name: gadC [H]

Synonym: CPE2060

Alternate gene names: 18311042

Gene position: 2355445-2354027 (Counterclockwise)

Preceding gene: 18311043

Following gene: 18311041

Centisome position: 77.7

GC content: 32.28

Gene sequence:

>1419_bases
ATGCCAAGTAAATCAAGTGCAAATGCAAAAACACTTACTTTGTTTGGGTTCTTTGCTATGACAGCATCAATGGTAATGAC
AGTATATGAATACCCTACATTTGCAACATCAGGATTTCATTTAGTATTTTTCTTAATCGTAGGCGGACTTTTATGGTTCT
TACCAGTTGCATTATGTGCAGCAGAAATGGCAACTGTAGATGGATGGGAAGAAGGTGGTATCTTTGCTTGGGTAGGTAAT
ACTTTAGGTGAAAGATGGGGATTCGCAGCGATCTTCTTCCAATGGTTCCAAATAACAGTTGGATTTGTTACAATGATTTA
TTTTATATTAGGGGCTCTATCTTATGTGCTAAATTGGCCAGCATTAAATAGTAATCCATTAATAAAATTTATAGGCGTTT
TAATCATATTCTGGGGATTAACTTTCTCACAATTTGGAGGAACTAAAAATACAGCTAAAATAGCTAAAGCAGGATTTGTT
TTCGGAGTTGTAGTTCCGGCAATTATATTATTTATATTAGGTATAATGTACATAGCAAAAGGTAATCCAGTACACGTTGA
TTTAAGTGCACATGCTCTTATACCTGACTTTACAAAGGTAAATACATTAGTTGTATTCGTATCATTTATATTAGCATACA
TGGGAGTTGAAGCATCAGCATCACATGTTAATAAATTAGAAAATGCAAGCAAAAACTATCCATTAGCTATGTTTATACTT
GTTGTATTAGCGATAGTTCTTAATACAGTAGGTGGTTTAACAGTAGCAGCAGTTATTCCTGCTGAGCAATTAAACTTAAG
TGCTGGTGTTGTTCAAACATTCCATCAATTAGTAGTTGTAAACTTAGGTGAAAGTTTTGATTGGATAACAAGAATAGTAG
CTTTACTATTAGCTTTAGGAGTTATGGCAGAAGTAAGTTCATGGGTTGTAGGTCCTTCAGAAGGTATGTATGCAGCAGCT
AAAAAAGGATTATTACCTAAAAAATTAACTGAAGTTAATAATCATGAAGTTCCAGTTCCATTAGTATTAGTTCAAGGTTT
AGTAGTTACTATATGGGCAGCTGTTTTAACATTTGGCGGTGGTGGAAATAACGTTTCATTCCTTACTGCAATATCATTAA
CAGTTGTTATATACTTAGTTGGATATTTATTATTCTTCATAGGATATATTATTTTAATATTAAAACATGGAGATTTAAAA
CGTGCTTATCATGTACCAGGTGGAAAAACATTTAAGATGATAGTAGCAATAGCAGGATTCGCTGTATCAGTATTTGCTTT
AGTTATTTCATTCGTACCACCATCACAATTAACAGGAAAAAGTGTATCAGAATATTTAACTATACTATCAATAAGTTTCA
TAGTAACAGTTTTAATTCCATTTATAATTTATGCTTTACATGATAAATGGAATAAATAG

Upstream 100 bases:

>100_bases
TTTTGTCTTAGGTAATAGTTGTTAATTGTTGAAACTTAGGTATTAAACTTAAGTTTCTAAAATTATAAATTAAACAATAA
CAACAGAGGAGGCGTTTATT

Downstream 100 bases:

>100_bases
CAGAAATAAATTAGAGTTATATTTGAAGTAGCCTAAAAGTATAGGCTACTTCAAACATTTTATAGAGAAATAATTAAAAG
TTTTGAAATTTTAACTATGT

Product: glutamate gamma-aminobutyrate antiporter

Products: L-glutamate [Cytoplasm]; 4-aminobutyrate [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 472; Mature: 471

Protein sequence:

>472_residues
MPSKSSANAKTLTLFGFFAMTASMVMTVYEYPTFATSGFHLVFFLIVGGLLWFLPVALCAAEMATVDGWEEGGIFAWVGN
TLGERWGFAAIFFQWFQITVGFVTMIYFILGALSYVLNWPALNSNPLIKFIGVLIIFWGLTFSQFGGTKNTAKIAKAGFV
FGVVVPAIILFILGIMYIAKGNPVHVDLSAHALIPDFTKVNTLVVFVSFILAYMGVEASASHVNKLENASKNYPLAMFIL
VVLAIVLNTVGGLTVAAVIPAEQLNLSAGVVQTFHQLVVVNLGESFDWITRIVALLLALGVMAEVSSWVVGPSEGMYAAA
KKGLLPKKLTEVNNHEVPVPLVLVQGLVVTIWAAVLTFGGGGNNVSFLTAISLTVVIYLVGYLLFFIGYIILILKHGDLK
RAYHVPGGKTFKMIVAIAGFAVSVFALVISFVPPSQLTGKSVSEYLTILSISFIVTVLIPFIIYALHDKWNK

Sequences:

>Translated_472_residues
MPSKSSANAKTLTLFGFFAMTASMVMTVYEYPTFATSGFHLVFFLIVGGLLWFLPVALCAAEMATVDGWEEGGIFAWVGN
TLGERWGFAAIFFQWFQITVGFVTMIYFILGALSYVLNWPALNSNPLIKFIGVLIIFWGLTFSQFGGTKNTAKIAKAGFV
FGVVVPAIILFILGIMYIAKGNPVHVDLSAHALIPDFTKVNTLVVFVSFILAYMGVEASASHVNKLENASKNYPLAMFIL
VVLAIVLNTVGGLTVAAVIPAEQLNLSAGVVQTFHQLVVVNLGESFDWITRIVALLLALGVMAEVSSWVVGPSEGMYAAA
KKGLLPKKLTEVNNHEVPVPLVLVQGLVVTIWAAVLTFGGGGNNVSFLTAISLTVVIYLVGYLLFFIGYIILILKHGDLK
RAYHVPGGKTFKMIVAIAGFAVSVFALVISFVPPSQLTGKSVSEYLTILSISFIVTVLIPFIIYALHDKWNK
>Mature_471_residues
PSKSSANAKTLTLFGFFAMTASMVMTVYEYPTFATSGFHLVFFLIVGGLLWFLPVALCAAEMATVDGWEEGGIFAWVGNT
LGERWGFAAIFFQWFQITVGFVTMIYFILGALSYVLNWPALNSNPLIKFIGVLIIFWGLTFSQFGGTKNTAKIAKAGFVF
GVVVPAIILFILGIMYIAKGNPVHVDLSAHALIPDFTKVNTLVVFVSFILAYMGVEASASHVNKLENASKNYPLAMFILV
VLAIVLNTVGGLTVAAVIPAEQLNLSAGVVQTFHQLVVVNLGESFDWITRIVALLLALGVMAEVSSWVVGPSEGMYAAAK
KGLLPKKLTEVNNHEVPVPLVLVQGLVVTIWAAVLTFGGGGNNVSFLTAISLTVVIYLVGYLLFFIGYIILILKHGDLKR
AYHVPGGKTFKMIVAIAGFAVSVFALVISFVPPSQLTGKSVSEYLTILSISFIVTVLIPFIIYALHDKWNK

Specific function: Involved in glutamate-dependent acid resistance. May be involved in antiport of glutamate and glutamate-gamma- aminobutyrate (GABA) [H]

COG id: COG0531

COG function: function code E; Amino acid transporters

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amino acid-polyamine-organocation (APC) superfamily. Glutamate:GABA antiporter (GGA) (TC 2.A.3.7) family [H]

Homologues:

Organism=Escherichia coli, GI1787768, Length=470, Percent_Identity=57.0212765957447, Blast_Score=554, Evalue=1e-159,
Organism=Escherichia coli, GI48994921, Length=433, Percent_Identity=22.4018475750577, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI87082380, Length=467, Percent_Identity=24.1970021413276, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI87081798, Length=423, Percent_Identity=22.9314420803783, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI2367353, Length=342, Percent_Identity=23.3918128654971, Blast_Score=65, Evalue=9e-12,
Organism=Escherichia coli, GI1786908, Length=310, Percent_Identity=23.8709677419355, Blast_Score=62, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004841
- InterPro:   IPR002293
- InterPro:   IPR004759 [H]

Pfam domain/function: PF00324 AA_permease [H]

EC number: NA

Molecular weight: Translated: 51057; Mature: 50926

Theoretical pI: Translated: 9.26; Mature: 9.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSKSSANAKTLTLFGFFAMTASMVMTVYEYPTFATSGFHLVFFLIVGGLLWFLPVALCA
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
AEMATVDGWEEGGIFAWVGNTLGERWGFAAIFFQWFQITVGFVTMIYFILGALSYVLNWP
HHHHHCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
ALNSNPLIKFIGVLIIFWGLTFSQFGGTKNTAKIAKAGFVFGVVVPAIILFILGIMYIAK
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GNPVHVDLSAHALIPDFTKVNTLVVFVSFILAYMGVEASASHVNKLENASKNYPLAMFIL
CCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHH
VVLAIVLNTVGGLTVAAVIPAEQLNLSAGVVQTFHQLVVVNLGESFDWITRIVALLLALG
HHHHHHHHHCCCHHHHEECCHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
VMAEVSSWVVGPSEGMYAAAKKGLLPKKLTEVNNHEVPVPLVLVQGLVVTIWAAVLTFGG
HHHHHHHHCCCCCCCCHHHHHCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
GGNNVSFLTAISLTVVIYLVGYLLFFIGYIILILKHGDLKRAYHVPGGKTFKMIVAIAGF
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCHHHHHHHHHHH
AVSVFALVISFVPPSQLTGKSVSEYLTILSISFIVTVLIPFIIYALHDKWNK
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
PSKSSANAKTLTLFGFFAMTASMVMTVYEYPTFATSGFHLVFFLIVGGLLWFLPVALCA
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
AEMATVDGWEEGGIFAWVGNTLGERWGFAAIFFQWFQITVGFVTMIYFILGALSYVLNWP
HHHHHCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
ALNSNPLIKFIGVLIIFWGLTFSQFGGTKNTAKIAKAGFVFGVVVPAIILFILGIMYIAK
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GNPVHVDLSAHALIPDFTKVNTLVVFVSFILAYMGVEASASHVNKLENASKNYPLAMFIL
CCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHH
VVLAIVLNTVGGLTVAAVIPAEQLNLSAGVVQTFHQLVVVNLGESFDWITRIVALLLALG
HHHHHHHHHCCCHHHHEECCHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
VMAEVSSWVVGPSEGMYAAAKKGLLPKKLTEVNNHEVPVPLVLVQGLVVTIWAAVLTFGG
HHHHHHHHCCCCCCCCHHHHHCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
GGNNVSFLTAISLTVVIYLVGYLLFFIGYIILILKHGDLKRAYHVPGGKTFKMIVAIAGF
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCHHHHHHHHHHH
AVSVFALVISFVPPSQLTGKSVSEYLTILSISFIVTVLIPFIIYALHDKWNK
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 4-aminobutyrate [Cytoplasm]; L-glutamate [Periplasm] [C]

Specific reaction: 4-aminobutyrate [Cytoplasm] + L-glutamate [Periplasm] = L-glutamate [Cytoplasm] + 4-aminobutyrate [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11337471; 10411264 [H]