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The map label for this gene is gadB [H]

Identifier: 18311040

GI number: 18311040

Start: 2350872

End: 2352266

Strand: Reverse

Name: gadB [H]

Synonym: CPE2058

Alternate gene names: 18311040

Gene position: 2352266-2350872 (Counterclockwise)

Preceding gene: 18311041

Following gene: 18311039

Centisome position: 77.6

GC content: 31.47

Gene sequence:

>1395_bases
ATGCTTTTTGGAAAAAATGAATTAGGTAGTCAATTTGAAACACCAATATTTGGTACTGTGGAAAGTAATGAATCTATTCC
TAAATATGAATTAGCTAAAAAATCAATTGCACCACAAGTTGCATATAGATTAATTAAGGATGACTTACTAGATGAAGGTA
ATGCTCGTCAAAACTTATGTACTTTCTGTCAAACTTACATGGATGATGAAGCTGTAAAATTAATGGCAGAAACATTAGAA
AAAAATGCTATAGATAAATCAGAATATCCTCAAACAACTGATTTAGAAAATCGTTGTGTAAATATATTAGCAGATTTATG
GCATGCACCAAAGGATGAAAGTTTCATGGGAACTTCAACAGTTGGATCATCAGAAGCTTGTATGCTTGGTGGTATGGCTA
TGAAATTTAGATGGAGAAATAGAGCTAAAGCTTTAGGTATGGATGTTACATCAAGAAAACCAAACTTAGTAATTTCTTCA
GGATACCAAGTATGTTGGGAAAAATTCTGTGTTTACTGGGATATAGAAATGAGATTAGTTCCAATGGACGAAGAACATAT
GAGCATAAATGTAGATAAAGTTTTAGACTATGTTGATGATTATACAATTGGTGTAGTTGGAATACTAGGAATTACTTACA
CTGGTAAATATGATGATATTAAAGCATTAGATAAAAAACTTGAAGAGTATAATAAAACTGCAAAATTAACAGTTCCAATG
CATATAGATGCTGCTTCAGGTGGTATGTTTGCTCCATTTATAGAACCAGAACTAGAGTGGGACTTCAGACTTAAAAACGT
TGTTTCAATAAGTACATCAGGTCATAAATATGGACTAGTTTATCCTGGTATAGGTTGGGTATTATGGAGAGATAAAGAAT
ACTTACCACAAGAATTAGTATTTGACGTAAGCTACTTAGGAGGAAAAATACCAACAATGGCAATTAACTTCTCAAGATCA
GCTAGCCAAATTATAGGTCAATATTATAACTTCTTAAGATATGGATTTGAAGGATATAGACAAATACATCAAAGAACAAA
AGATGTTGCTATGTTCTTAGCATCTGAACTTGAAAAAACTGGATTATTCCAAATATACAATAATGGTGAAAACTTACCAA
TAGTTTGTTATAAATTAAGAGATGATGTAAATGTTGATTGGACATTATATGATCTAGCAGATAGATTATTAATGAAAGGA
TGGCAAGTACCAGCTTACCCATTACCAGCAGACTTACAAGATACAATAATTCAAAGAATTGTTTGTCGTGCTGACCTTAG
CCATAACTTAGCTGAATTATACTTAAGAGACTTAAAAGCAGCTATAGAAGATTTAAATAACGCAAGAGTTTTAAATCAAA
AATCTAAAACAGGAGTACAAGGATTTACTCACTAG

Upstream 100 bases:

>100_bases
TATAGATAAAATGATTTATATAACTAAAAATTACTCTTAATTTCTTGAAAGTTATGAAGAGATTAAGAGTGGTTTTTAGC
TAAATTAGGAGGTATTTAAA

Downstream 100 bases:

>100_bases
TATATAATAAAAAACTCTCCTTAAAGTAAAAACTTTAGGAGAGTTTTTTCTATTATGAAGTATATTTACTAGAAATATTA
TTTTCTATTGATTATAATAT

Product: glutamate decarboxylase

Products: NA

Alternate protein names: GAD-beta [H]

Number of amino acids: Translated: 464; Mature: 464

Protein sequence:

>464_residues
MLFGKNELGSQFETPIFGTVESNESIPKYELAKKSIAPQVAYRLIKDDLLDEGNARQNLCTFCQTYMDDEAVKLMAETLE
KNAIDKSEYPQTTDLENRCVNILADLWHAPKDESFMGTSTVGSSEACMLGGMAMKFRWRNRAKALGMDVTSRKPNLVISS
GYQVCWEKFCVYWDIEMRLVPMDEEHMSINVDKVLDYVDDYTIGVVGILGITYTGKYDDIKALDKKLEEYNKTAKLTVPM
HIDAASGGMFAPFIEPELEWDFRLKNVVSISTSGHKYGLVYPGIGWVLWRDKEYLPQELVFDVSYLGGKIPTMAINFSRS
ASQIIGQYYNFLRYGFEGYRQIHQRTKDVAMFLASELEKTGLFQIYNNGENLPIVCYKLRDDVNVDWTLYDLADRLLMKG
WQVPAYPLPADLQDTIIQRIVCRADLSHNLAELYLRDLKAAIEDLNNARVLNQKSKTGVQGFTH

Sequences:

>Translated_464_residues
MLFGKNELGSQFETPIFGTVESNESIPKYELAKKSIAPQVAYRLIKDDLLDEGNARQNLCTFCQTYMDDEAVKLMAETLE
KNAIDKSEYPQTTDLENRCVNILADLWHAPKDESFMGTSTVGSSEACMLGGMAMKFRWRNRAKALGMDVTSRKPNLVISS
GYQVCWEKFCVYWDIEMRLVPMDEEHMSINVDKVLDYVDDYTIGVVGILGITYTGKYDDIKALDKKLEEYNKTAKLTVPM
HIDAASGGMFAPFIEPELEWDFRLKNVVSISTSGHKYGLVYPGIGWVLWRDKEYLPQELVFDVSYLGGKIPTMAINFSRS
ASQIIGQYYNFLRYGFEGYRQIHQRTKDVAMFLASELEKTGLFQIYNNGENLPIVCYKLRDDVNVDWTLYDLADRLLMKG
WQVPAYPLPADLQDTIIQRIVCRADLSHNLAELYLRDLKAAIEDLNNARVLNQKSKTGVQGFTH
>Mature_464_residues
MLFGKNELGSQFETPIFGTVESNESIPKYELAKKSIAPQVAYRLIKDDLLDEGNARQNLCTFCQTYMDDEAVKLMAETLE
KNAIDKSEYPQTTDLENRCVNILADLWHAPKDESFMGTSTVGSSEACMLGGMAMKFRWRNRAKALGMDVTSRKPNLVISS
GYQVCWEKFCVYWDIEMRLVPMDEEHMSINVDKVLDYVDDYTIGVVGILGITYTGKYDDIKALDKKLEEYNKTAKLTVPM
HIDAASGGMFAPFIEPELEWDFRLKNVVSISTSGHKYGLVYPGIGWVLWRDKEYLPQELVFDVSYLGGKIPTMAINFSRS
ASQIIGQYYNFLRYGFEGYRQIHQRTKDVAMFLASELEKTGLFQIYNNGENLPIVCYKLRDDVNVDWTLYDLADRLLMKG
WQVPAYPLPADLQDTIIQRIVCRADLSHNLAELYLRDLKAAIEDLNNARVLNQKSKTGVQGFTH

Specific function: Converts internalized glutamate to GABA and increases the internal pH. Involved in glutamate-dependent acid resistance in gastric fluid [H]

COG id: COG0076

COG function: function code E; Glutamate decarboxylase and related PLP-dependent proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the group II decarboxylase family [H]

Homologues:

Organism=Homo sapiens, GI31982936, Length=446, Percent_Identity=22.4215246636771, Blast_Score=98, Evalue=1e-20,
Organism=Escherichia coli, GI1789934, Length=450, Percent_Identity=47.5555555555556, Blast_Score=434, Evalue=1e-123,
Organism=Escherichia coli, GI1787769, Length=445, Percent_Identity=47.8651685393258, Blast_Score=434, Evalue=1e-123,
Organism=Caenorhabditis elegans, GI17557272, Length=357, Percent_Identity=25.2100840336134, Blast_Score=111, Evalue=9e-25,
Organism=Caenorhabditis elegans, GI25148342, Length=364, Percent_Identity=25.5494505494505, Blast_Score=106, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17543922, Length=409, Percent_Identity=24.2053789731051, Blast_Score=102, Evalue=5e-22,
Organism=Saccharomyces cerevisiae, GI6323905, Length=465, Percent_Identity=38.0645161290323, Blast_Score=305, Evalue=1e-83,
Organism=Saccharomyces cerevisiae, GI6320500, Length=405, Percent_Identity=22.2222222222222, Blast_Score=90, Evalue=6e-19,
Organism=Drosophila melanogaster, GI21355963, Length=435, Percent_Identity=23.6781609195402, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24654344, Length=435, Percent_Identity=23.6781609195402, Blast_Score=110, Evalue=2e-24,

Paralogues:

None

Copy number: 3820 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010107
- InterPro:   IPR002129
- InterPro:   IPR015424
- InterPro:   IPR015421 [H]

Pfam domain/function: PF00282 Pyridoxal_deC [H]

EC number: =4.1.1.15 [H]

Molecular weight: Translated: 52940; Mature: 52940

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLFGKNELGSQFETPIFGTVESNESIPKYELAKKSIAPQVAYRLIKDDLLDEGNARQNLC
CCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
TFCQTYMDDEAVKLMAETLEKNAIDKSEYPQTTDLENRCVNILADLWHAPKDESFMGTST
HHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCC
VGSSEACMLGGMAMKFRWRNRAKALGMDVTSRKPNLVISSGYQVCWEKFCVYWDIEMRLV
CCCCCHHHHCCCCEEEEHHHHHHHCCCCCCCCCCCEEEECCHHHHHHHEEEEEEEEEEEE
PMDEEHMSINVDKVLDYVDDYTIGVVGILGITYTGKYDDIKALDKKLEEYNKTAKLTVPM
CCCCCHHEEEHHHHHHHHCCHHHHHHHHHHEEECCCCHHHHHHHHHHHHHCCCEEEEEEE
HIDAASGGMFAPFIEPELEWDFRLKNVVSISTSGHKYGLVYPGIGWVLWRDKEYLPQELV
EEECCCCCEECCCCCCCCCCCEEECEEEEEECCCCEEEEEECCCCEEEECCCHHCCHHHH
FDVSYLGGKIPTMAINFSRSASQIIGQYYNFLRYGFEGYRQIHQRTKDVAMFLASELEKT
HHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GLFQIYNNGENLPIVCYKLRDDVNVDWTLYDLADRLLMKGWQVPAYPLPADLQDTIIQRI
CEEEEEECCCCCCEEEEEECCCCCCCEEHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHH
VCRADLSHNLAELYLRDLKAAIEDLNNARVLNQKSKTGVQGFTH
HHHHHHCHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCC
>Mature Secondary Structure
MLFGKNELGSQFETPIFGTVESNESIPKYELAKKSIAPQVAYRLIKDDLLDEGNARQNLC
CCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
TFCQTYMDDEAVKLMAETLEKNAIDKSEYPQTTDLENRCVNILADLWHAPKDESFMGTST
HHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCC
VGSSEACMLGGMAMKFRWRNRAKALGMDVTSRKPNLVISSGYQVCWEKFCVYWDIEMRLV
CCCCCHHHHCCCCEEEEHHHHHHHCCCCCCCCCCCEEEECCHHHHHHHEEEEEEEEEEEE
PMDEEHMSINVDKVLDYVDDYTIGVVGILGITYTGKYDDIKALDKKLEEYNKTAKLTVPM
CCCCCHHEEEHHHHHHHHCCHHHHHHHHHHEEECCCCHHHHHHHHHHHHHCCCEEEEEEE
HIDAASGGMFAPFIEPELEWDFRLKNVVSISTSGHKYGLVYPGIGWVLWRDKEYLPQELV
EEECCCCCEECCCCCCCCCCCEEECEEEEEECCCCEEEEEECCCCEEEECCCHHCCHHHH
FDVSYLGGKIPTMAINFSRSASQIIGQYYNFLRYGFEGYRQIHQRTKDVAMFLASELEKT
HHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GLFQIYNNGENLPIVCYKLRDDVNVDWTLYDLADRLLMKGWQVPAYPLPADLQDTIIQRI
CEEEEEECCCCCCEEEEEECCCCCCCEEHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHH
VCRADLSHNLAELYLRDLKAAIEDLNNARVLNQKSKTGVQGFTH
HHHHHHCHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]