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The map label for this gene is rsmE [H]

Identifier: 18311008

GI number: 18311008

Start: 2317301

End: 2318053

Strand: Reverse

Name: rsmE [H]

Synonym: CPE2026

Alternate gene names: 18311008

Gene position: 2318053-2317301 (Counterclockwise)

Preceding gene: 18311009

Following gene: 18311007

Centisome position: 76.47

GC content: 31.47

Gene sequence:

>753_bases
ATGCATAAGTTTTTTACACCAGCGCATATGTTTTCAGACACTCATGCAAAAATAACAGGGGAAGATGTAAAACATATTTA
TAAGGTTTTAAGGGTAAAACCAGGTGAGAAGGTTGTTGTAAATAACCTTGAAAGTGAAGAGTTTTTAGGTGAGGTAGAAG
AGGTTAATAAACAAGAGGTTTCTATCAAAATCTTAGAGAAGCTTGATGTAAACAATGAAAGTAATTTAAATATTACTTTA
TTTCAAGGAATGCCAAAGGCTGCAAAAATGGATTTAATAGTTCAAAAATGTGTAGAACTTGGTGTTTCAGAAATAATTCC
AACAATCACTGATAGAGTAGACGTTAAATTAAAAGGTGAATTTAAAAAATTAGATAGATTAAATAAAATCGCTTTAGAGG
CATGTAAACAAAGTAAAAGAACAGTAATACCAGAAGTATTAGAACCAATGGACTTTAAAGGGGTTTTAGAAAAGATAGAT
GAATTAGACCTTATGGTTATTCCTTATGAAAATGCTACAGGATACGGAATAAGAGCTATGGTTAACTCTATAGAGAATAA
AGATAGTATAAAAAATGTTGGAATAATGGTTGGGCCAGAAGGCGGATTTGAAGAAAGTGAAATAGAAGCCCTAAAAGAAA
AAGGAGCTCATATTGTAACTTTAGGACCAAGAATTTTAAGAACAGAGACAGCAGGATTTACCTGCATTTCTCTTATTCAG
TATGAGCTTGGTGATTTAGGAGGAAATATTTAA

Upstream 100 bases:

>100_bases
TTAGAACAAGAAGGTTTTGAGGTTATGGAAGTTAACAAAGATGGAGAATGGAACTGTATTGTTGCTAAGTTAAAATAATT
TAGTAAGGGAGTATAAAATT

Downstream 100 bases:

>100_bases
TGAAAGTTGCATTTGCTACACTAGGGTGTAGAGTTAATACATATGAATCAGAAGCGATGACTGAAAAATTTGTTAGGGAA
GGCTATGAAGTTGTAGATTT

Product: 16S ribosomal RNA methyltransferase RsmE

Products: NA

Alternate protein names: 16S rRNA m3U1498 methyltransferase [H]

Number of amino acids: Translated: 250; Mature: 250

Protein sequence:

>250_residues
MHKFFTPAHMFSDTHAKITGEDVKHIYKVLRVKPGEKVVVNNLESEEFLGEVEEVNKQEVSIKILEKLDVNNESNLNITL
FQGMPKAAKMDLIVQKCVELGVSEIIPTITDRVDVKLKGEFKKLDRLNKIALEACKQSKRTVIPEVLEPMDFKGVLEKID
ELDLMVIPYENATGYGIRAMVNSIENKDSIKNVGIMVGPEGGFEESEIEALKEKGAHIVTLGPRILRTETAGFTCISLIQ
YELGDLGGNI

Sequences:

>Translated_250_residues
MHKFFTPAHMFSDTHAKITGEDVKHIYKVLRVKPGEKVVVNNLESEEFLGEVEEVNKQEVSIKILEKLDVNNESNLNITL
FQGMPKAAKMDLIVQKCVELGVSEIIPTITDRVDVKLKGEFKKLDRLNKIALEACKQSKRTVIPEVLEPMDFKGVLEKID
ELDLMVIPYENATGYGIRAMVNSIENKDSIKNVGIMVGPEGGFEESEIEALKEKGAHIVTLGPRILRTETAGFTCISLIQ
YELGDLGGNI
>Mature_250_residues
MHKFFTPAHMFSDTHAKITGEDVKHIYKVLRVKPGEKVVVNNLESEEFLGEVEEVNKQEVSIKILEKLDVNNESNLNITL
FQGMPKAAKMDLIVQKCVELGVSEIIPTITDRVDVKLKGEFKKLDRLNKIALEACKQSKRTVIPEVLEPMDFKGVLEKID
ELDLMVIPYENATGYGIRAMVNSIENKDSIKNVGIMVGPEGGFEESEIEALKEKGAHIVTLGPRILRTETAGFTCISLIQ
YELGDLGGNI

Specific function: Specifically methylates the uridine in position 1498 of 16S rRNA in the fully assembled 30S ribosomal subunit [H]

COG id: COG1385

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase RsmE family [H]

Homologues:

Organism=Escherichia coli, GI87082186, Length=249, Percent_Identity=34.5381526104418, Blast_Score=140, Evalue=8e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015947
- InterPro:   IPR006700 [H]

Pfam domain/function: PF04452 Methyltrans_RNA [H]

EC number: =2.1.1.193 [H]

Molecular weight: Translated: 27897; Mature: 27897

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHKFFTPAHMFSDTHAKITGEDVKHIYKVLRVKPGEKVVVNNLESEEFLGEVEEVNKQEV
CCCCCCHHHHHCCCCCEECHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHCCHHHH
SIKILEKLDVNNESNLNITLFQGMPKAAKMDLIVQKCVELGVSEIIPTITDRVDVKLKGE
HHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCHHHHHHHHHCCEEEEECCH
FKKLDRLNKIALEACKQSKRTVIPEVLEPMDFKGVLEKIDELDLMVIPYENATGYGIRAM
HHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCHHHHHHHHCCCCEEEEECCCCCCCHHHHH
VNSIENKDSIKNVGIMVGPEGGFEESEIEALKEKGAHIVTLGPRILRTETAGFTCISLIQ
HHHHCCHHHHHHCCEEECCCCCCCHHHHHHHHHCCCEEEEECCEEEEECCCCHHHHHHHH
YELGDLGGNI
HHHHHCCCCC
>Mature Secondary Structure
MHKFFTPAHMFSDTHAKITGEDVKHIYKVLRVKPGEKVVVNNLESEEFLGEVEEVNKQEV
CCCCCCHHHHHCCCCCEECHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHCCHHHH
SIKILEKLDVNNESNLNITLFQGMPKAAKMDLIVQKCVELGVSEIIPTITDRVDVKLKGE
HHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCHHHHHHHHHCCEEEEECCH
FKKLDRLNKIALEACKQSKRTVIPEVLEPMDFKGVLEKIDELDLMVIPYENATGYGIRAM
HHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCHHHHHHHHCCCCEEEEECCCCCCCHHHHH
VNSIENKDSIKNVGIMVGPEGGFEESEIEALKEKGAHIVTLGPRILRTETAGFTCISLIQ
HHHHCCHHHHHHCCEEECCCCCCCHHHHHHHHHCCCEEEEECCEEEEECCCCHHHHHHHH
YELGDLGGNI
HHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]