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The map label for this gene is aspS

Identifier: 18310915

GI number: 18310915

Start: 2213854

End: 2215647

Strand: Reverse

Name: aspS

Synonym: CPE1933

Alternate gene names: 18310915

Gene position: 2215647-2213854 (Counterclockwise)

Preceding gene: 18310916

Following gene: 18310914

Centisome position: 73.09

GC content: 32.27

Gene sequence:

>1794_bases
ATGGGTGAAGCTTTAAATGGATTAAAGCGTAACATAATGTGTGGCGACGCTAGAGAAAGCCATATTGGACAAAAAGTAAC
CGTAATGGGTTGGGTTCAAAGAAATAGAAATCTTGGAGGTCTTCAATTTATAGACTTAAGAGATAGAGAAGGAATTTTAC
AGGTTGTATTTAATGATGATTTAGGAGAAGAAATCCTAGAAAAAGCAAAATCAATAAGACCTGAATATTGTATTGCTGTA
ACAGGTGAAATAGTTAAGAGAGAATCAGTAAATCCAAACATGCCAACAGGTATGGTTGAGTTAAAAGCTGAGGAATTAAA
GATTTTATCTGAATCAGATACTCCTCCAATATATATAAAAGAAGATTTAGATGCTGCTGAAAGTATTAGATTAAAGTATA
GATACTTAGATTTAAGAAGACCAGATATGCAAAATATCTTCAAAATAAGACATAAGACAACTAAAGCAATAAGAGATTAT
TTAGATCAAAATGGTTTCTTAGAAATGGAGACGCCAATACTTACAAAGTCAACTCCAGAAGGAGCTAGAGACTATCTTGT
TCCATCAAGAAACTATCCAGGAATGTTCTATGCATTACCTCAATCACCACAATTATTTAAACAATTATTAATGGTATCAG
GTTTTGATAGATACTTCCAAATAGTTAAGTGTTTCAGAGATGAAGACTTAAGAGCTAATAGACAACCAGAGTTTACTCAA
GTTGACTTAGAAATGTCATTTGTTGAGCAAGATGATGTTATGGCTTTAAATGAAGGTTTAATAAAACATGTATTTAAAGA
AGTTTTAGGAGTAGATGTAAAAACTCCAATAAAGAGAATGACATTTAAAGATGCTATGGAAAAATACGGTTCAGATAAAC
CTGACTTAAGATTTGGAATGGAAATCACAAACTTAAGTGATGTTGTTAAAGAATGTGGATTTAAAGTATTCACAGACGCT
GTAGCTAATGGCGGTTCTGTTAGAGGTTTATGCTTAGAAGGCGGAGCTTCTATGGGAAGAAAAGACATAGATAGATTAGG
AGAGTTCGTTAAAACTTTCAAAGCTAAAGGATTAGCATGGATTCAATTAAAAGAAGAGGGTGTTAAATCACCAATAGCTA
AATTCTTTAGTGAAGAAGAGCTAAACAAAATAATTGAAACTATGGGAGCTAAAACAGGAGATTTAATCCTTATAGTTGCT
GATAAAAACTCAGTAGTTTTAAAAGCTTTAGGAGAATTAAGATTAGAACTTTCAAGAAAATTTGATTTAGTTAAAGATAA
GAGTGAGTTTAACTTCACATGGATAACAGAGTTTGATCTTCTTGAGTACGATGAAGAAGAAGGAAGATACTTTGCAGCTC
ACCATCCATTTACAATGCCAATGGATGAGGATATAAAGTATTTAGATACTGATCCAGGAAGAGTTAGAGCTAAGGCTTAT
GACTTAGTATTAAATGGAGAAGAGTTAGGTGGAGGATCTATAAGAATACATGATACTAAGCTTCAAGAAAAAATGTTTGA
AGTATTAGGATTTACTCAAGAATCAGCTTGGGAAAGATTTGGATTCTTATTAGAAGCATTTAAATTTGGACCACCACCAC
ACGGCGGATTAGCTTTCGGTTTAGATAGAATGATAATGTTCTTAGCAGGAACTGAAAATATCAAGGATGTTATAACATTC
CCTAAAAACCAAAATGCATTCTGTTATTTAACTGAAGCACCTAATATAGTAGATGAAGAACAATTAAAAGAATTAGGAAT
TGAAACAATAAAGAAAGAAGATACGGCAGAATAA

Upstream 100 bases:

>100_bases
TTAAAATTAAGAGAATGAGCGATGGAGAACAATTCGAAGTCAGCTTAGATATAAATGAAATAGTAAATATAGTTAAGTAG
TAGACAGGAGGAGAATTAAA

Downstream 100 bases:

>100_bases
TTTTTGAGCAATCTAAATTGAGATACTAAAGTAAACTTAATTAGCGTGGGTTTTTTACTCACGCTAATTTTAGTCAATTT
ATATGGAAAATGAGTAAAAA

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS

Number of amino acids: Translated: 597; Mature: 596

Protein sequence:

>597_residues
MGEALNGLKRNIMCGDARESHIGQKVTVMGWVQRNRNLGGLQFIDLRDREGILQVVFNDDLGEEILEKAKSIRPEYCIAV
TGEIVKRESVNPNMPTGMVELKAEELKILSESDTPPIYIKEDLDAAESIRLKYRYLDLRRPDMQNIFKIRHKTTKAIRDY
LDQNGFLEMETPILTKSTPEGARDYLVPSRNYPGMFYALPQSPQLFKQLLMVSGFDRYFQIVKCFRDEDLRANRQPEFTQ
VDLEMSFVEQDDVMALNEGLIKHVFKEVLGVDVKTPIKRMTFKDAMEKYGSDKPDLRFGMEITNLSDVVKECGFKVFTDA
VANGGSVRGLCLEGGASMGRKDIDRLGEFVKTFKAKGLAWIQLKEEGVKSPIAKFFSEEELNKIIETMGAKTGDLILIVA
DKNSVVLKALGELRLELSRKFDLVKDKSEFNFTWITEFDLLEYDEEEGRYFAAHHPFTMPMDEDIKYLDTDPGRVRAKAY
DLVLNGEELGGGSIRIHDTKLQEKMFEVLGFTQESAWERFGFLLEAFKFGPPPHGGLAFGLDRMIMFLAGTENIKDVITF
PKNQNAFCYLTEAPNIVDEEQLKELGIETIKKEDTAE

Sequences:

>Translated_597_residues
MGEALNGLKRNIMCGDARESHIGQKVTVMGWVQRNRNLGGLQFIDLRDREGILQVVFNDDLGEEILEKAKSIRPEYCIAV
TGEIVKRESVNPNMPTGMVELKAEELKILSESDTPPIYIKEDLDAAESIRLKYRYLDLRRPDMQNIFKIRHKTTKAIRDY
LDQNGFLEMETPILTKSTPEGARDYLVPSRNYPGMFYALPQSPQLFKQLLMVSGFDRYFQIVKCFRDEDLRANRQPEFTQ
VDLEMSFVEQDDVMALNEGLIKHVFKEVLGVDVKTPIKRMTFKDAMEKYGSDKPDLRFGMEITNLSDVVKECGFKVFTDA
VANGGSVRGLCLEGGASMGRKDIDRLGEFVKTFKAKGLAWIQLKEEGVKSPIAKFFSEEELNKIIETMGAKTGDLILIVA
DKNSVVLKALGELRLELSRKFDLVKDKSEFNFTWITEFDLLEYDEEEGRYFAAHHPFTMPMDEDIKYLDTDPGRVRAKAY
DLVLNGEELGGGSIRIHDTKLQEKMFEVLGFTQESAWERFGFLLEAFKFGPPPHGGLAFGLDRMIMFLAGTENIKDVITF
PKNQNAFCYLTEAPNIVDEEQLKELGIETIKKEDTAE
>Mature_596_residues
GEALNGLKRNIMCGDARESHIGQKVTVMGWVQRNRNLGGLQFIDLRDREGILQVVFNDDLGEEILEKAKSIRPEYCIAVT
GEIVKRESVNPNMPTGMVELKAEELKILSESDTPPIYIKEDLDAAESIRLKYRYLDLRRPDMQNIFKIRHKTTKAIRDYL
DQNGFLEMETPILTKSTPEGARDYLVPSRNYPGMFYALPQSPQLFKQLLMVSGFDRYFQIVKCFRDEDLRANRQPEFTQV
DLEMSFVEQDDVMALNEGLIKHVFKEVLGVDVKTPIKRMTFKDAMEKYGSDKPDLRFGMEITNLSDVVKECGFKVFTDAV
ANGGSVRGLCLEGGASMGRKDIDRLGEFVKTFKAKGLAWIQLKEEGVKSPIAKFFSEEELNKIIETMGAKTGDLILIVAD
KNSVVLKALGELRLELSRKFDLVKDKSEFNFTWITEFDLLEYDEEEGRYFAAHHPFTMPMDEDIKYLDTDPGRVRAKAYD
LVLNGEELGGGSIRIHDTKLQEKMFEVLGFTQESAWERFGFLLEAFKFGPPPHGGLAFGLDRMIMFLAGTENIKDVITFP
KNQNAFCYLTEAPNIVDEEQLKELGIETIKKEDTAE

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI40789249, Length=598, Percent_Identity=40.3010033444816, Blast_Score=438, Evalue=1e-123,
Organism=Homo sapiens, GI4758762, Length=288, Percent_Identity=29.5138888888889, Blast_Score=89, Evalue=8e-18,
Organism=Homo sapiens, GI194272210, Length=289, Percent_Identity=27.3356401384083, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI5031815, Length=287, Percent_Identity=27.5261324041812, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI226491502, Length=263, Percent_Identity=28.8973384030418, Blast_Score=86, Evalue=8e-17,
Organism=Homo sapiens, GI45439306, Length=240, Percent_Identity=27.0833333333333, Blast_Score=78, Evalue=2e-14,
Organism=Escherichia coli, GI1788173, Length=595, Percent_Identity=49.9159663865546, Blast_Score=582, Evalue=1e-167,
Organism=Escherichia coli, GI1789256, Length=278, Percent_Identity=28.4172661870504, Blast_Score=108, Evalue=9e-25,
Organism=Escherichia coli, GI1790571, Length=329, Percent_Identity=26.7477203647416, Blast_Score=105, Evalue=6e-24,
Organism=Escherichia coli, GI1787161, Length=279, Percent_Identity=24.3727598566308, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI32566633, Length=598, Percent_Identity=35.2842809364548, Blast_Score=322, Evalue=4e-88,
Organism=Caenorhabditis elegans, GI71994340, Length=187, Percent_Identity=31.0160427807487, Blast_Score=94, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17535925, Length=187, Percent_Identity=31.0160427807487, Blast_Score=93, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17535927, Length=187, Percent_Identity=31.0160427807487, Blast_Score=93, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI71984122, Length=284, Percent_Identity=28.169014084507, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17551876, Length=270, Percent_Identity=28.1481481481481, Blast_Score=85, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6325153, Length=622, Percent_Identity=33.6012861736334, Blast_Score=291, Evalue=1e-79,
Organism=Saccharomyces cerevisiae, GI6321807, Length=287, Percent_Identity=27.8745644599303, Blast_Score=89, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6323011, Length=250, Percent_Identity=30.8, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6320242, Length=145, Percent_Identity=30.3448275862069, Blast_Score=76, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6324256, Length=139, Percent_Identity=27.3381294964029, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24584738, Length=602, Percent_Identity=35.7142857142857, Blast_Score=350, Evalue=1e-96,
Organism=Drosophila melanogaster, GI17136276, Length=239, Percent_Identity=29.7071129707113, Blast_Score=96, Evalue=6e-20,
Organism=Drosophila melanogaster, GI24640849, Length=257, Percent_Identity=27.6264591439689, Blast_Score=87, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24640851, Length=257, Percent_Identity=27.6264591439689, Blast_Score=86, Evalue=6e-17,
Organism=Drosophila melanogaster, GI19921528, Length=281, Percent_Identity=27.7580071174377, Blast_Score=71, Evalue=2e-12,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYD_CLOP1 (Q0TP28)

Other databases:

- EMBL:   CP000246
- RefSeq:   YP_696612.1
- ProteinModelPortal:   Q0TP28
- SMR:   Q0TP28
- STRING:   Q0TP28
- GeneID:   4201595
- GenomeReviews:   CP000246_GR
- KEGG:   cpf:CPF_2188
- TIGR:   CPF_2188
- eggNOG:   COG0173
- HOGENOM:   HBG396032
- OMA:   AFPKTQQ
- ProtClustDB:   PRK00476
- BioCyc:   CPER195103:CPF_2188-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00044_B
- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365
- Gene3D:   G3DSA:3.30.1360.30
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR22594
- PANTHER:   PTHR22594:SF5
- PRINTS:   PR01042
- TIGRFAMs:   TIGR00459

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti; SSF50249 Nucleic_acid_OB

EC number: =6.1.1.12

Molecular weight: Translated: 68027; Mature: 67896

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGEALNGLKRNIMCGDARESHIGQKVTVMGWVQRNRNLGGLQFIDLRDREGILQVVFNDD
CCHHHHHHHHCCCCCCCHHHHCCCEEEEEEHHHHCCCCCCEEEEEECCCCCEEEEEECCC
LGEEILEKAKSIRPEYCIAVTGEIVKRESVNPNMPTGMVELKAEELKILSESDTPPIYIK
CCHHHHHHHHHCCCCEEEEECHHHHHHCCCCCCCCCCEEEEEHHHHHHCCCCCCCCEEEE
EDLDAAESIRLKYRYLDLRRPDMQNIFKIRHKTTKAIRDYLDQNGFLEMETPILTKSTPE
CCCCCHHHHEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEECCCCC
GARDYLVPSRNYPGMFYALPQSPQLFKQLLMVSGFDRYFQIVKCFRDEDLRANRQPEFTQ
CCHHCCCCCCCCCCEEEECCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCEE
VDLEMSFVEQDDVMALNEGLIKHVFKEVLGVDVKTPIKRMTFKDAMEKYGSDKPDLRFGM
EEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCEEECE
EITNLSDVVKECGFKVFTDAVANGGSVRGLCLEGGASMGRKDIDRLGEFVKTFKAKGLAW
EECCHHHHHHHCCCCEEHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCEEE
IQLKEEGVKSPIAKFFSEEELNKIIETMGAKTGDLILIVADKNSVVLKALGELRLELSRK
EEECHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHH
FDLVKDKSEFNFTWITEFDLLEYDEEEGRYFAAHHPFTMPMDEDIKYLDTDPGRVRAKAY
HHHHCCCCCCCEEEEEEEHHHCCCCCCCCEEEECCCEECCCCCCCCEECCCCCCEEEEEE
DLVLNGEELGGGSIRIHDTKLQEKMFEVLGFTQESAWERFGFLLEAFKFGPPPHGGLAFG
EEEECCCCCCCCEEEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHH
LDRMIMFLAGTENIKDVITFPKNQNAFCYLTEAPNIVDEEQLKELGIETIKKEDTAE
HHHHHHHHCCCCCCHHHEECCCCCCCEEEEECCCCCCCHHHHHHHCHHHHCCCCCCC
>Mature Secondary Structure 
GEALNGLKRNIMCGDARESHIGQKVTVMGWVQRNRNLGGLQFIDLRDREGILQVVFNDD
CHHHHHHHHCCCCCCCHHHHCCCEEEEEEHHHHCCCCCCEEEEEECCCCCEEEEEECCC
LGEEILEKAKSIRPEYCIAVTGEIVKRESVNPNMPTGMVELKAEELKILSESDTPPIYIK
CCHHHHHHHHHCCCCEEEEECHHHHHHCCCCCCCCCCEEEEEHHHHHHCCCCCCCCEEEE
EDLDAAESIRLKYRYLDLRRPDMQNIFKIRHKTTKAIRDYLDQNGFLEMETPILTKSTPE
CCCCCHHHHEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEECCCCC
GARDYLVPSRNYPGMFYALPQSPQLFKQLLMVSGFDRYFQIVKCFRDEDLRANRQPEFTQ
CCHHCCCCCCCCCCEEEECCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCEE
VDLEMSFVEQDDVMALNEGLIKHVFKEVLGVDVKTPIKRMTFKDAMEKYGSDKPDLRFGM
EEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCEEECE
EITNLSDVVKECGFKVFTDAVANGGSVRGLCLEGGASMGRKDIDRLGEFVKTFKAKGLAW
EECCHHHHHHHCCCCEEHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCEEE
IQLKEEGVKSPIAKFFSEEELNKIIETMGAKTGDLILIVADKNSVVLKALGELRLELSRK
EEECHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHH
FDLVKDKSEFNFTWITEFDLLEYDEEEGRYFAAHHPFTMPMDEDIKYLDTDPGRVRAKAY
HHHHCCCCCCCEEEEEEEHHHCCCCCCCCEEEECCCEECCCCCCCCEECCCCCCEEEEEE
DLVLNGEELGGGSIRIHDTKLQEKMFEVLGFTQESAWERFGFLLEAFKFGPPPHGGLAFG
EEEECCCCCCCCEEEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHH
LDRMIMFLAGTENIKDVITFPKNQNAFCYLTEAPNIVDEEQLKELGIETIKKEDTAE
HHHHHHHHCCCCCCHHHEECCCCCCCEEEEECCCCCCCHHHHHHHCHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA