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The map label for this gene is nifS [H]

Identifier: 18310767

GI number: 18310767

Start: 2064248

End: 2065444

Strand: Reverse

Name: nifS [H]

Synonym: CPE1785

Alternate gene names: 18310767

Gene position: 2065444-2064248 (Counterclockwise)

Preceding gene: 18310768

Following gene: 18310766

Centisome position: 68.13

GC content: 32.58

Gene sequence:

>1197_bases
ATGAATAATAGAGTTGTTTATATGGACTACTCAGCAACTACATATGTTAAGCCAGAAGTATTAGAAGAAATGTTACCATA
TTTCACAAATAAGTTTGGAAATCCATCAGCATTTTACGGAGTTTCAAGAGAATCAAGAATGGCTGTAGATACTGCTAGAG
AAAGAGTAGCTAAAGTATTAAATGCTGATACAAATGAAATCTACTTTACTGGTGGCGGATCAGAAGCAGATAACTGGGCA
ATAAAAGGAATAGCTTTTGCTCATAAAAATAAAGGAAATCATATAATAACTACAAAAATAGAGCACCATGCTGTATTACA
TACTTGCCAATGGTTAGAAAAACAAGGCTTTAAAGTAACTTACTTAGATGTAAATGAAGAAGGTTTTGTTGATTTAGAAG
AATTAAAAAATGCTATTACTGATAAAACTATCTTAGTTTCTGTAATGTTTGCAAACAATGAAATAGGAACTATAGAGCCA
GTTAAGGAAATAGGAAAAATTTGTAGAGAAAGAAAAGTAATATTCCATACAGATGCAGTTCAAGCTGTAGGAAATGTAAA
GATAGATGTTAAAGATATGAACATCGATTTACTTTCATTAGCTGGACATAAAGTTTATGGACCAAAAGGAATCGGAGCTT
TATATATAAGAAAAGGTATAAGAATAGATAACTTAATCCACGGTGGTGGTCAAGAGAGAGCTAGAAGAGCTGGAACTGAG
AACATACCTGCAATAGTTGGATTAGGAAAGGCTATGGAAATAGCTGGAGAAAACTTAGATGAGCATATAGCTAAAATTTC
TAAGTTAAGAGATAAGTTAATAAAAGGATTATTAGAAGTACCATTTACAAGATTAAACGGACCAAAAGATGGTAGCAAGA
GATTACCAGGTAACGTAAATGTATGCTTTGAATTCATTGAAGGTGAAGGAATTCTTCTTTCATTAGACTTTGAAGGAATT
TGTGGTTCAAGTGGAAGTGCTTGTACATCAGGATCATTAGATCCATCACACGTGTTATTAGCAATAGGTTTACCTCATGA
AATAGCACACGGATCATTAAGATTAAGTTTAGGTGAAGGTACAACTGAAGAAGACGTTGATTACGTATTAGAAAAAGTAC
CACCAATAATCGCAAGATTAAGAAGTATGTCACCATTATGGAAAAATCATTTAAGAGAAGTAGAAGGAGAGAATTAA

Upstream 100 bases:

>100_bases
GAGGTTACTAATTCAGTAACACTTAAAGATATAGTAGAAGATTATAAAGCTATGAAAGAAAAAAATGAAGCTTTAAAAAT
TGTTAGTAGGAGTGAAGAAA

Downstream 100 bases:

>100_bases
TTATGATATATAGTGAAAAAGTTATGGATCATTTCAAAAACCCAAGAAATGTAGGAGAAATTAAAGACGCTAACGGAATC
GGAGAAGTTGGTAACGCTAA

Product: cysteine desulfurase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 398; Mature: 398

Protein sequence:

>398_residues
MNNRVVYMDYSATTYVKPEVLEEMLPYFTNKFGNPSAFYGVSRESRMAVDTARERVAKVLNADTNEIYFTGGGSEADNWA
IKGIAFAHKNKGNHIITTKIEHHAVLHTCQWLEKQGFKVTYLDVNEEGFVDLEELKNAITDKTILVSVMFANNEIGTIEP
VKEIGKICRERKVIFHTDAVQAVGNVKIDVKDMNIDLLSLAGHKVYGPKGIGALYIRKGIRIDNLIHGGGQERARRAGTE
NIPAIVGLGKAMEIAGENLDEHIAKISKLRDKLIKGLLEVPFTRLNGPKDGSKRLPGNVNVCFEFIEGEGILLSLDFEGI
CGSSGSACTSGSLDPSHVLLAIGLPHEIAHGSLRLSLGEGTTEEDVDYVLEKVPPIIARLRSMSPLWKNHLREVEGEN

Sequences:

>Translated_398_residues
MNNRVVYMDYSATTYVKPEVLEEMLPYFTNKFGNPSAFYGVSRESRMAVDTARERVAKVLNADTNEIYFTGGGSEADNWA
IKGIAFAHKNKGNHIITTKIEHHAVLHTCQWLEKQGFKVTYLDVNEEGFVDLEELKNAITDKTILVSVMFANNEIGTIEP
VKEIGKICRERKVIFHTDAVQAVGNVKIDVKDMNIDLLSLAGHKVYGPKGIGALYIRKGIRIDNLIHGGGQERARRAGTE
NIPAIVGLGKAMEIAGENLDEHIAKISKLRDKLIKGLLEVPFTRLNGPKDGSKRLPGNVNVCFEFIEGEGILLSLDFEGI
CGSSGSACTSGSLDPSHVLLAIGLPHEIAHGSLRLSLGEGTTEEDVDYVLEKVPPIIARLRSMSPLWKNHLREVEGEN
>Mature_398_residues
MNNRVVYMDYSATTYVKPEVLEEMLPYFTNKFGNPSAFYGVSRESRMAVDTARERVAKVLNADTNEIYFTGGGSEADNWA
IKGIAFAHKNKGNHIITTKIEHHAVLHTCQWLEKQGFKVTYLDVNEEGFVDLEELKNAITDKTILVSVMFANNEIGTIEP
VKEIGKICRERKVIFHTDAVQAVGNVKIDVKDMNIDLLSLAGHKVYGPKGIGALYIRKGIRIDNLIHGGGQERARRAGTE
NIPAIVGLGKAMEIAGENLDEHIAKISKLRDKLIKGLLEVPFTRLNGPKDGSKRLPGNVNVCFEFIEGEGILLSLDFEGI
CGSSGSACTSGSLDPSHVLLAIGLPHEIAHGSLRLSLGEGTTEEDVDYVLEKVPPIIARLRSMSPLWKNHLREVEGEN

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=389, Percent_Identity=49.3573264781491, Blast_Score=386, Evalue=1e-107,
Organism=Homo sapiens, GI156713448, Length=418, Percent_Identity=35.4066985645933, Blast_Score=238, Evalue=1e-62,
Organism=Escherichia coli, GI48994898, Length=395, Percent_Identity=47.3417721518987, Blast_Score=368, Evalue=1e-103,
Organism=Escherichia coli, GI1787970, Length=345, Percent_Identity=24.9275362318841, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI1789175, Length=400, Percent_Identity=26.5, Blast_Score=100, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI25143064, Length=388, Percent_Identity=47.1649484536082, Blast_Score=356, Evalue=1e-98,
Organism=Caenorhabditis elegans, GI17533177, Length=327, Percent_Identity=34.8623853211009, Blast_Score=178, Evalue=4e-45,
Organism=Saccharomyces cerevisiae, GI6319831, Length=389, Percent_Identity=48.8431876606684, Blast_Score=377, Evalue=1e-105,
Organism=Drosophila melanogaster, GI20129463, Length=389, Percent_Identity=47.8149100257069, Blast_Score=362, Evalue=1e-100,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR010240
- InterPro:   IPR017772
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 43818; Mature: 43818

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: PS00018 EF_HAND_1 ; PS00595 AA_TRANSFER_CLASS_5 ; PS00142 ZINC_PROTEASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNRVVYMDYSATTYVKPEVLEEMLPYFTNKFGNPSAFYGVSRESRMAVDTARERVAKVL
CCCEEEEEECCCCEEECHHHHHHHHHHHHCCCCCCCCEECCCCCCHHHHHHHHHHHHHHH
NADTNEIYFTGGGSEADNWAIKGIAFAHKNKGNHIITTKIEHHAVLHTCQWLEKQGFKVT
CCCCCEEEEECCCCCCCCEEEEEEEEEECCCCCEEEEEEHHHHHHHHHHHHHHHCCCEEE
YLDVNEEGFVDLEELKNAITDKTILVSVMFANNEIGTIEPVKEIGKICRERKVIFHTDAV
EEEECCCCCEEHHHHHHHHCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHCEEEEEEHHH
QAVGNVKIDVKDMNIDLLSLAGHKVYGPKGIGALYIRKGIRIDNLIHGGGQERARRAGTE
HHHCCEEEEEEECCEEEEEECCCEEECCCCCCEEEEECCCCCCCEECCCCHHHHHHCCCC
NIPAIVGLGKAMEIAGENLDEHIAKISKLRDKLIKGLLEVPFTRLNGPKDGSKRLPGNVN
CCCCEEECCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCCE
VCFEFIEGEGILLSLDFEGICGSSGSACTSGSLDPSHVLLAIGLPHEIAHGSLRLSLGEG
EEEEEECCCEEEEEEECCCCCCCCCCCCCCCCCCCCCEEEEECCCHHHCCCEEEEEECCC
TTEEDVDYVLEKVPPIIARLRSMSPLWKNHLREVEGEN
CCHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHCCCCC
>Mature Secondary Structure
MNNRVVYMDYSATTYVKPEVLEEMLPYFTNKFGNPSAFYGVSRESRMAVDTARERVAKVL
CCCEEEEEECCCCEEECHHHHHHHHHHHHCCCCCCCCEECCCCCCHHHHHHHHHHHHHHH
NADTNEIYFTGGGSEADNWAIKGIAFAHKNKGNHIITTKIEHHAVLHTCQWLEKQGFKVT
CCCCCEEEEECCCCCCCCEEEEEEEEEECCCCCEEEEEEHHHHHHHHHHHHHHHCCCEEE
YLDVNEEGFVDLEELKNAITDKTILVSVMFANNEIGTIEPVKEIGKICRERKVIFHTDAV
EEEECCCCCEEHHHHHHHHCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHCEEEEEEHHH
QAVGNVKIDVKDMNIDLLSLAGHKVYGPKGIGALYIRKGIRIDNLIHGGGQERARRAGTE
HHHCCEEEEEEECCEEEEEECCCEEECCCCCCEEEEECCCCCCCEECCCCHHHHHHCCCC
NIPAIVGLGKAMEIAGENLDEHIAKISKLRDKLIKGLLEVPFTRLNGPKDGSKRLPGNVN
CCCCEEECCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCCE
VCFEFIEGEGILLSLDFEGICGSSGSACTSGSLDPSHVLLAIGLPHEIAHGSLRLSLGEG
EEEEEECCCEEEEEEECCCCCCCCCCCCCCCCCCCCCEEEEECCCHHHCCCEEEEEECCC
TTEEDVDYVLEKVPPIIARLRSMSPLWKNHLREVEGEN
CCHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA