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The map label for this gene is ackB

Identifier: 18310706

GI number: 18310706

Start: 2000425

End: 2001621

Strand: Reverse

Name: ackB

Synonym: CPE1724

Alternate gene names: 18310706

Gene position: 2001621-2000425 (Counterclockwise)

Preceding gene: 18310707

Following gene: 18310705

Centisome position: 66.03

GC content: 31.91

Gene sequence:

>1197_bases
ATGAACGTATTAGTTATAAATTGCGGAAGCTCATCATTAAAATATCAATTAATAGATATGGATACTGAAAATGCTTTAGC
AACTGGATTAGTAGAAAGAATAGGATTAGAAGGCGCAAACTTAACTCAAAAGAGTGAAGGTAAAGATAAATATGAAATCG
TAGAGCCAATGAAAGATCATCAAGATGCTATTAGATTAGTTCTAGGAGCTTTAATAGATGAAAAACATGGTGTTATAAAA
TCATTAGATGAAATAAATGCTATAGGACACAGAGTTGTTCATGGTGGAGAAAAATATGCTGAATCTGCATTAGTTACAGA
GGAAGTTATGAAAGATTTAGAAGAGTGTGCTAAATTAGCTCCACTTCATAATCCTGCAAATATAATAGGAATAAATGCTT
GTAAAGCATTAATGCCAAATGTTCCTATGGTTGTTGTATTTGATACAGCATTCCATCAAACAATGCCAGAAAAAGCATTC
GTATATGCATTACCATATGAATTATATGAAAAAGAACACATAAGAAAATATGGTTTCCACGGAACTTCTCATAAATATGT
TTCAGCTAAAATAGCAGAAGCTATGGGTAAAAACATAGAAGATTTAAAAATAATCACTTGTCACTTAGGAAATGGTGCAA
GTATAGCAGCTATAAAAAATGGTAAATGTGTAGATACTACTATGGGATTCACACCACTTGAAGGTTTAGTAATGGGAACT
AGATGTGGAAATATAGACCCAGCTGTAGTTACATACTTAATAGATGAATTAGGATATACTTCACAAGAAGTAAATACTTT
AATGAACAAAAAATCAGGAATATTTGGAGTTTCAGGAGTAAGCAGTGACTTTAGAGATGTTGAAGCAGCTGCTGACAAAG
GAAGCAAAGAAGCTCAAATAGCTTTAGATTTATTCAGAAACTCAGTTAAGAAATATATAGGAGCATACATTGCAGAGATG
AATGGATGTGACGTAATAGTATTTACTGCTGGTGTTGGAGAAAACTCAATCATAGAAAGAGGAGCTATCTGTAGAGATTT
AGAATTCTTAGGAATTGAGTTAGATGAAGAAAGAAACAATATAAGAGCTAAAGTTGCTGAAATAAGCAAAGAGGGTTCAA
GAATTAAACTTTTCGTAGTTCCAACTAACGAAGAATTAATGATAGCACAAGATACAGTAAGTATTGTAAGTAAATAA

Upstream 100 bases:

>100_bases
AACTAAATAGTAATTGATTAGAATTTTATATAGATGGATAAAATTAAAATGAATTAAGCTTATTAATTTTATATTATAAG
TTTAACAGGAGGAGTTTATA

Downstream 100 bases:

>100_bases
TTATAAAATACTTGACTTTTAAATATCTAGTTAATATAATAAGATATGTGTTGTTTAGGAATAAACAATGCATATCTTAT
GTTAAGGAGCGTGAAAGTAG

Product: acetate kinase

Products: NA

Alternate protein names: Acetokinase 2

Number of amino acids: Translated: 398; Mature: 398

Protein sequence:

>398_residues
MNVLVINCGSSSLKYQLIDMDTENALATGLVERIGLEGANLTQKSEGKDKYEIVEPMKDHQDAIRLVLGALIDEKHGVIK
SLDEINAIGHRVVHGGEKYAESALVTEEVMKDLEECAKLAPLHNPANIIGINACKALMPNVPMVVVFDTAFHQTMPEKAF
VYALPYELYEKEHIRKYGFHGTSHKYVSAKIAEAMGKNIEDLKIITCHLGNGASIAAIKNGKCVDTTMGFTPLEGLVMGT
RCGNIDPAVVTYLIDELGYTSQEVNTLMNKKSGIFGVSGVSSDFRDVEAAADKGSKEAQIALDLFRNSVKKYIGAYIAEM
NGCDVIVFTAGVGENSIIERGAICRDLEFLGIELDEERNNIRAKVAEISKEGSRIKLFVVPTNEELMIAQDTVSIVSK

Sequences:

>Translated_398_residues
MNVLVINCGSSSLKYQLIDMDTENALATGLVERIGLEGANLTQKSEGKDKYEIVEPMKDHQDAIRLVLGALIDEKHGVIK
SLDEINAIGHRVVHGGEKYAESALVTEEVMKDLEECAKLAPLHNPANIIGINACKALMPNVPMVVVFDTAFHQTMPEKAF
VYALPYELYEKEHIRKYGFHGTSHKYVSAKIAEAMGKNIEDLKIITCHLGNGASIAAIKNGKCVDTTMGFTPLEGLVMGT
RCGNIDPAVVTYLIDELGYTSQEVNTLMNKKSGIFGVSGVSSDFRDVEAAADKGSKEAQIALDLFRNSVKKYIGAYIAEM
NGCDVIVFTAGVGENSIIERGAICRDLEFLGIELDEERNNIRAKVAEISKEGSRIKLFVVPTNEELMIAQDTVSIVSK
>Mature_398_residues
MNVLVINCGSSSLKYQLIDMDTENALATGLVERIGLEGANLTQKSEGKDKYEIVEPMKDHQDAIRLVLGALIDEKHGVIK
SLDEINAIGHRVVHGGEKYAESALVTEEVMKDLEECAKLAPLHNPANIIGINACKALMPNVPMVVVFDTAFHQTMPEKAF
VYALPYELYEKEHIRKYGFHGTSHKYVSAKIAEAMGKNIEDLKIITCHLGNGASIAAIKNGKCVDTTMGFTPLEGLVMGT
RCGNIDPAVVTYLIDELGYTSQEVNTLMNKKSGIFGVSGVSSDFRDVEAAADKGSKEAQIALDLFRNSVKKYIGAYIAEM
NGCDVIVFTAGVGENSIIERGAICRDLEFLGIELDEERNNIRAKVAEISKEGSRIKLFVVPTNEELMIAQDTVSIVSK

Specific function: Involved In The Activation Of Acetate To Acetyl CoA And The Secretion Of Acetate. During Anaerobic Growth Of The Organism, This Enzyme Is Also Involved In The Synthesis Of Most Of The ATP Formed Catabolically. [C]

COG id: COG0282

COG function: function code C; Acetate kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acetokinase family

Homologues:

Organism=Escherichia coli, GI1788633, Length=402, Percent_Identity=46.5174129353234, Blast_Score=351, Evalue=5e-98,
Organism=Escherichia coli, GI145693184, Length=395, Percent_Identity=40.7594936708861, Blast_Score=312, Evalue=3e-86,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): ACKA2_CLOPE (Q8XJN2)

Other databases:

- EMBL:   BA000016
- RefSeq:   NP_562640.1
- ProteinModelPortal:   Q8XJN2
- SMR:   Q8XJN2
- GeneID:   990034
- GenomeReviews:   BA000016_GR
- KEGG:   cpe:CPE1724
- NMPDR:   fig|195102.1.peg.1787
- HOGENOM:   HBG285863
- OMA:   SSDFRDV
- PhylomeDB:   Q8XJN2
- ProtClustDB:   PRK00180
- BioCyc:   CPER195102:CPE1724-MONOMER
- BRENDA:   2.7.2.1
- GO:   GO:0005737
- HAMAP:   MF_00020
- InterPro:   IPR000890
- InterPro:   IPR004372
- PANTHER:   PTHR21060
- PANTHER:   PTHR21060:SF11
- PIRSF:   PIRSF000722
- PRINTS:   PR00471
- TIGRFAMs:   TIGR00016

Pfam domain/function: PF00871 Acetate_kinase

EC number: =2.7.2.1

Molecular weight: Translated: 43418; Mature: 43418

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS01075 ACETATE_KINASE_1; PS01076 ACETATE_KINASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVLVINCGSSSLKYQLIDMDTENALATGLVERIGLEGANLTQKSEGKDKYEIVEPMKDH
CEEEEEECCCCCEEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHH
QDAIRLVLGALIDEKHGVIKSLDEINAIGHRVVHGGEKYAESALVTEEVMKDLEECAKLA
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHC
PLHNPANIIGINACKALMPNVPMVVVFDTAFHQTMPEKAFVYALPYELYEKEHIRKYGFH
CCCCCCCEEEHHHHHHHCCCCCEEEEEECHHHHCCCCCEEEEECCHHHHHHHHHHHHCCC
GTSHKYVSAKIAEAMGKNIEDLKIITCHLGNGASIAAIKNGKCVDTTMGFTPLEGLVMGT
CCCCHHHHHHHHHHHCCCCCCEEEEEEECCCCCEEEEECCCEEEECCCCCCCCCCEEEEC
RCGNIDPAVVTYLIDELGYTSQEVNTLMNKKSGIFGVSGVSSDFRDVEAAADKGSKEAQI
CCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHCCCCCHHHH
ALDLFRNSVKKYIGAYIAEMNGCDVIVFTAGVGENSIIERGAICRDLEFLGIELDEERNN
HHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHCCCHHHCHHEECEEECCCCCH
IRAKVAEISKEGSRIKLFVVPTNEELMIAQDTVSIVSK
HHHHHHHHHCCCCEEEEEEEECCCCEEEEEHHHHHHCC
>Mature Secondary Structure
MNVLVINCGSSSLKYQLIDMDTENALATGLVERIGLEGANLTQKSEGKDKYEIVEPMKDH
CEEEEEECCCCCEEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHH
QDAIRLVLGALIDEKHGVIKSLDEINAIGHRVVHGGEKYAESALVTEEVMKDLEECAKLA
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHC
PLHNPANIIGINACKALMPNVPMVVVFDTAFHQTMPEKAFVYALPYELYEKEHIRKYGFH
CCCCCCCEEEHHHHHHHCCCCCEEEEEECHHHHCCCCCEEEEECCHHHHHHHHHHHHCCC
GTSHKYVSAKIAEAMGKNIEDLKIITCHLGNGASIAAIKNGKCVDTTMGFTPLEGLVMGT
CCCCHHHHHHHHHHHCCCCCCEEEEEEECCCCCEEEEECCCEEEECCCCCCCCCCEEEEC
RCGNIDPAVVTYLIDELGYTSQEVNTLMNKKSGIFGVSGVSSDFRDVEAAADKGSKEAQI
CCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHCCCCCHHHH
ALDLFRNSVKKYIGAYIAEMNGCDVIVFTAGVGENSIIERGAICRDLEFLGIELDEERNN
HHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHCCCHHHCHHEECEEECCCCCH
IRAKVAEISKEGSRIKLFVVPTNEELMIAQDTVSIVSK
HHHHHHHHHCCCCEEEEEEEECCCCEEEEEHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11792842