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The map label for this gene is dapG [H]

Identifier: 18310660

GI number: 18310660

Start: 1954579

End: 1955775

Strand: Reverse

Name: dapG [H]

Synonym: CPE1678

Alternate gene names: 18310660

Gene position: 1955775-1954579 (Counterclockwise)

Preceding gene: 18310661

Following gene: 18310659

Centisome position: 64.52

GC content: 30.91

Gene sequence:

>1197_bases
TTGAAAATAGTAGTACAAAAATTTGGTGGTACTTCTGTATCAACAAAAGAAAGAAGAACTATGGTTGTAGAAAAGGTAAA
AGGGGCAATAAATAAAGGATATTTACCAGTGGTTGTTGTATCTGCTATGGGAAGAAAAGGAGAACCTTATGCAACTGATA
CACTTTTAGGATTAGTATCAGAAGAGTTTAAAAAAGAAAATAAATTAGCTACTGATTTACTTATGGGATGTGGAGAAATT
ATAAGCACTGTTGTCATGAGTGATGAATTAAGAGAGGCAGAAATAGAAGCAGTTCCTTTAACAGGAGGAAATGCAGGAAT
ACTTACAGATGATAATTATTCAAGTGCTGATTTTATAGATATAAATCCTAAATTAATATTAGAAGTTCTTAAAGAAGGAA
AAGTGCCTGTTGTTGCTGGGTTCCAAGGAGTTGATAGAAATGGATTCTTAACTACTTTAGGAAGAGGCGGAAGTGATACT
ACGGCAGCGGTTTTAGGGGTAGCATTAAAGGCTGAAGAAATTGAAATATATACAGATGTTGATGGAATAATGACTGCTGA
CCCAAGAATAGTTGGAGATGCTGAGCTTATAAATAAAATAAGTTATAATGAAGTATTCCAATTAGCAGATCAAGGGGCTA
AGGTAATTCATCCAAGAGCTGTAGAAATAGCAATGAAAGGAAATGTAACCTTAGTAATAAAGAATACTATGAGTACTTGC
ATTGGTACAATGATAGATAGTCTAGGGGATGTAGATAATAATAAGTTCATAACTGGAATAACTCATCAAGGAAATAGAAT
TCAAGTTTCTATAAAATCTGATGACAATAAAGATAATATAAATTATAAGACTATTTTGGAAAGCCTTGCAAATAATAAAA
TAAGTTTAGACTTAATTAATATCTTCCCTAAAGAAAAAGTATTTACAATAGATGCAGGTGTTAAAGAATTATTTGAAGAT
ATTATGAGAAAAAATAATTTAAAATATAGTTTAGTAGAAGATTTAAGCACTATAGCTATAGTTGGTTCAAGAATGAGAGG
AATTCCAGGAGTTATGGCTAAAATAGTAGGTGCTTTAGACAATGAAAACATAGAAGTTTTACAAACAGCAGATTCACATA
TGACAATTTGGTGTCTTGTTGAAAGTAAAAATGTAAGAGAAGCTATAAAAGCACTTCATAGAGTCTTTATGAAATAA

Upstream 100 bases:

>100_bases
ATCGAGTGGGAAAATGTAAAAAGAATAGGAGCTAAAACCATAATATTAGATGTAGATAGTGATATGATAAAAAAAACTAA
GCTGTAGGAGTGTGGGGATT

Downstream 100 bases:

>100_bases
TGTATATAAATTTCCTTTCTGTACAAAATAATTTTAGTACAGGAGGGATATATATGGAACATAACAAAGAAAATGAAAAT
AAAAGTATAGAAGAAATAAA

Product: aspartate kinase I

Products: NA

Alternate protein names: Aspartate kinase 1; Aspartokinase I [H]

Number of amino acids: Translated: 398; Mature: 398

Protein sequence:

>398_residues
MKIVVQKFGGTSVSTKERRTMVVEKVKGAINKGYLPVVVVSAMGRKGEPYATDTLLGLVSEEFKKENKLATDLLMGCGEI
ISTVVMSDELREAEIEAVPLTGGNAGILTDDNYSSADFIDINPKLILEVLKEGKVPVVAGFQGVDRNGFLTTLGRGGSDT
TAAVLGVALKAEEIEIYTDVDGIMTADPRIVGDAELINKISYNEVFQLADQGAKVIHPRAVEIAMKGNVTLVIKNTMSTC
IGTMIDSLGDVDNNKFITGITHQGNRIQVSIKSDDNKDNINYKTILESLANNKISLDLINIFPKEKVFTIDAGVKELFED
IMRKNNLKYSLVEDLSTIAIVGSRMRGIPGVMAKIVGALDNENIEVLQTADSHMTIWCLVESKNVREAIKALHRVFMK

Sequences:

>Translated_398_residues
MKIVVQKFGGTSVSTKERRTMVVEKVKGAINKGYLPVVVVSAMGRKGEPYATDTLLGLVSEEFKKENKLATDLLMGCGEI
ISTVVMSDELREAEIEAVPLTGGNAGILTDDNYSSADFIDINPKLILEVLKEGKVPVVAGFQGVDRNGFLTTLGRGGSDT
TAAVLGVALKAEEIEIYTDVDGIMTADPRIVGDAELINKISYNEVFQLADQGAKVIHPRAVEIAMKGNVTLVIKNTMSTC
IGTMIDSLGDVDNNKFITGITHQGNRIQVSIKSDDNKDNINYKTILESLANNKISLDLINIFPKEKVFTIDAGVKELFED
IMRKNNLKYSLVEDLSTIAIVGSRMRGIPGVMAKIVGALDNENIEVLQTADSHMTIWCLVESKNVREAIKALHRVFMK
>Mature_398_residues
MKIVVQKFGGTSVSTKERRTMVVEKVKGAINKGYLPVVVVSAMGRKGEPYATDTLLGLVSEEFKKENKLATDLLMGCGEI
ISTVVMSDELREAEIEAVPLTGGNAGILTDDNYSSADFIDINPKLILEVLKEGKVPVVAGFQGVDRNGFLTTLGRGGSDT
TAAVLGVALKAEEIEIYTDVDGIMTADPRIVGDAELINKISYNEVFQLADQGAKVIHPRAVEIAMKGNVTLVIKNTMSTC
IGTMIDSLGDVDNNKFITGITHQGNRIQVSIKSDDNKDNINYKTILESLANNKISLDLINIFPKEKVFTIDAGVKELFED
IMRKNNLKYSLVEDLSTIAIVGSRMRGIPGVMAKIVGALDNENIEVLQTADSHMTIWCLVESKNVREAIKALHRVFMK

Specific function: Catalyzes 2 nonconsecutive reactions in the common biosynthetic pathway leading from Asp to diaminopimelate and Lys, to Met, and to Thr and Ile. [C]

COG id: COG0527

COG function: function code E; Aspartokinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aspartokinase family [H]

Homologues:

Organism=Escherichia coli, GI1786183, Length=460, Percent_Identity=26.7391304347826, Blast_Score=121, Evalue=6e-29,
Organism=Escherichia coli, GI1790455, Length=339, Percent_Identity=28.3185840707965, Blast_Score=100, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6320893, Length=364, Percent_Identity=26.6483516483516, Blast_Score=96, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001048
- InterPro:   IPR005260
- InterPro:   IPR001341
- InterPro:   IPR018042 [H]

Pfam domain/function: PF00696 AA_kinase [H]

EC number: =2.7.2.4 [H]

Molecular weight: Translated: 43325; Mature: 43325

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: PS00324 ASPARTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIVVQKFGGTSVSTKERRTMVVEKVKGAINKGYLPVVVVSAMGRKGEPYATDTLLGLVS
CEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHEEECCCCCCCCCHHHHHHHHHH
EEFKKENKLATDLLMGCGEIISTVVMSDELREAEIEAVPLTGGNAGILTDDNYSSADFID
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCEEEECCCCCCCCEEE
INPKLILEVLKEGKVPVVAGFQGVDRNGFLTTLGRGGSDTTAAVLGVALKAEEIEIYTDV
CCHHHHHHHHHCCCCEEEECCCCCCCCCCEEECCCCCCCHHHHHHHHHEEECEEEEEECC
DGIMTADPRIVGDAELINKISYNEVFQLADQGAKVIHPRAVEIAMKGNVTLVIKNTMSTC
CCEEECCCCEECCHHHHHHCCHHHHHHHHHCCCEEECCCEEEEEEECCEEEEECCHHHHH
IGTMIDSLGDVDNNKFITGITHQGNRIQVSIKSDDNKDNINYKTILESLANNKISLDLIN
HHHHHHHHCCCCCCCEEEEEECCCCEEEEEEECCCCCCCCCHHHHHHHHHCCCEEEEEEE
IFPKEKVFTIDAGVKELFEDIMRKNNLKYSLVEDLSTIAIVGSRMRGIPGVMAKIVGALD
ECCCCEEEEECHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCC
NENIEVLQTADSHMTIWCLVESKNVREAIKALHRVFMK
CCCEEEEEECCCCEEEEEEEECCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MKIVVQKFGGTSVSTKERRTMVVEKVKGAINKGYLPVVVVSAMGRKGEPYATDTLLGLVS
CEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHEEECCCCCCCCCHHHHHHHHHH
EEFKKENKLATDLLMGCGEIISTVVMSDELREAEIEAVPLTGGNAGILTDDNYSSADFID
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCEEEECCCCCCCCEEE
INPKLILEVLKEGKVPVVAGFQGVDRNGFLTTLGRGGSDTTAAVLGVALKAEEIEIYTDV
CCHHHHHHHHHCCCCEEEECCCCCCCCCCEEECCCCCCCHHHHHHHHHEEECEEEEEECC
DGIMTADPRIVGDAELINKISYNEVFQLADQGAKVIHPRAVEIAMKGNVTLVIKNTMSTC
CCEEECCCCEECCHHHHHHCCHHHHHHHHHCCCEEECCCEEEEEEECCEEEEECCHHHHH
IGTMIDSLGDVDNNKFITGITHQGNRIQVSIKSDDNKDNINYKTILESLANNKISLDLIN
HHHHHHHHCCCCCCCEEEEEECCCCEEEEEEECCCCCCCCCHHHHHHHHHCCCEEEEEEE
IFPKEKVFTIDAGVKELFEDIMRKNNLKYSLVEDLSTIAIVGSRMRGIPGVMAKIVGALD
ECCCCEEEEECHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCC
NENIEVLQTADSHMTIWCLVESKNVREAIKALHRVFMK
CCCEEEEEECCCCEEEEEEEECCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8098035; 9384377 [H]