Click here to switch to the map view.

The map label for this gene is glgB

Identifier: 18310570

GI number: 18310570

Start: 1861666

End: 1863660

Strand: Reverse

Name: glgB

Synonym: CPE1588

Alternate gene names: 18310570

Gene position: 1863660-1861666 (Counterclockwise)

Preceding gene: 18310571

Following gene: 18310569

Centisome position: 61.48

GC content: 31.13

Gene sequence:

>1995_bases
ATGGGGGGAAAAGAGATGAGAAATTGTAAAGAACTTAAGCATGAGAAAAATGGTAACGTTACCGAAAAAGTGGGGAAAAA
CAAAGGAAAAAGCAAAAAAGTGTCTAAAGATGAAAGCTTATTAAGCTTTGATTTGTTTTTAGAGGGAAAAGAACATTCAG
CTTATAAGTTTATGGGAGCTCATTTTATAACTGAAAATAGAAAAAGGGGTGTAAGATTTACTACTTGGGCTCCAAGGGCT
AGCAAAATATATGTTATAGGTGATTTTAATAATTGGGAATTAAAAGAAGAATACTCCATGAAAAAAATTAATGAAAGAGG
AATATGGAGTTTATTTCTTCCTAAATTAGAAGAGGGTATAAAGTATAAATTTGCAGTAGTAAATGAATGTGGAAATAATA
CAGTATATAAGGCTGACCCATATGCATTTAAATCAGAATTAAGACCTAATACAGCCTCTGTACTTACTAAAATTAAGAGT
TTTAGATGGGGAGATAAGAGATGGCTTAATAAAAGAGAAAAAGAAGGTTTAGATAATAAACCTATGAATATATATGAACT
TCATTTAGGGTCTTGGAAGAGAAAAGATGGAGAGTTTATGACCTATGAAGAAATAAGTGAAGTGCTAGTAGAATATATAA
AAGAGATGGGATATACCCATGTTGAGTTCATGCCTATAAATGAACATCCCTTAGATGCTTCTTGGGGATATCAAGGAGTA
GGGTATTACTCAGTTACAAGTAGATATGGAGATTTAAATGGATTAAAGACTCTTATAAATAAACTTCATAAAAATAATAT
TGGAGTATTACTAGATTGGGTACCTAGCCATTTTTGTAAGGATGAGCATGGATTATTTATGTTTGATGGATCACCAACCT
ATGAATATGAGGCTTGGTGGAAGGCTAATAATGAAGGATGGGGAACTTGTAATTTTGATTTAGGAAGACCAGAGGTTAAA
TCCTTTTTATTTTCTAATGCTATGTACTGGATAAATGAATTTCATGTAGATGGATTAAGAGTAGATGCGGTATCTAATAT
GCTTTATTTAGATTATGGAAGAGAATATGGAGAATGGGAGCCAAATATCTATGGAGGAAATGGAAACCTTGAAGCTATTG
CCTTTTTAAAAGAACTAAATACAATTATAAAAAAAGAAGGAAAAGGAGCAATTACAGTAGCTGAAGAATCAACCTCATGG
GAAGGAATTACTAAACCTGTTGAAGAGGATGGATTAGGATTTGATTATAAATGGAATATGGGATGGATGAATGATACCTT
AAGTTATATTGAGTTAGATCCAATATATAGAAAGTATCATCACAATAAAATGAACTTTTCAATGATGTACAATTATTCAG
AAAAGTTTATTCTACCAATTTCACATGATGAGGTTGTTCATGGTAAGAAATCTCTTATAAATAAAATGTGGGGAGATGAT
TGGAAAAAGTATGCAGGACTAAGAGTTTATGCTTCTTTTATGATGGGACACCCAGGGAAAAAATTAATGTTTATGGGCTG
TGAATTTGGTCAGTTCGTTGAATGGAGAGAATGGGAAGAACTTCAATGGAATGTTATTGAAGAATTTGATATACACAGAA
AAACCAAGGAGTATTTTAAAGCATTAAATAAATTTTATTTAGAGAATAGTTCTTTATGGTCACTTGATTATGAAGAAGAA
GGCTTTAAATGGATTGATGCTGATAATTCAGAAGAAAGTGTACTTTCATTTATAAGAATTGGAAAGAATAAAAAGGAAAA
ATTAATATTTATATGTAATTTTACTCCAGAGGTTTATTATGATTTTAAAGTAGGGGTTCCAGAACTTGGAGAATATGTTG
AAGCCTTTAATTCAGATGCCTTAGAATTTGGAGGGGCAGGAAATATTGTGGGAGATAGTATTTTAAAAGCCACAGAGGAA
AGTTTTAAAGATTTTGATTATTCAATAAGTGTTAAGGTACCTCCATTAGGAACTTTAGTTTTGAAAGTAAAATAA

Upstream 100 bases:

>100_bases
TTGAATAACACCCATAATTAATTGTAATTTAATACCTAAAATAGTATAATTAAAGCAAAATATTGTGTTTATAATATTTT
GCTTTAATGAGAGGTTTACT

Downstream 100 bases:

>100_bases
GAAGGAAAAGGCTACTATAAATCTAAGTACATAATATTATTTTACTTTGGATAAATAGTAGCTTTTATTACATAAATAAT
TAATAGAGGAGCATTTTATA

Product: glycogen branching enzyme

Products: NA

Alternate protein names: 1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase 2; Glycogen-branching enzyme 2; BE 2

Number of amino acids: Translated: 664; Mature: 663

Protein sequence:

>664_residues
MGGKEMRNCKELKHEKNGNVTEKVGKNKGKSKKVSKDESLLSFDLFLEGKEHSAYKFMGAHFITENRKRGVRFTTWAPRA
SKIYVIGDFNNWELKEEYSMKKINERGIWSLFLPKLEEGIKYKFAVVNECGNNTVYKADPYAFKSELRPNTASVLTKIKS
FRWGDKRWLNKREKEGLDNKPMNIYELHLGSWKRKDGEFMTYEEISEVLVEYIKEMGYTHVEFMPINEHPLDASWGYQGV
GYYSVTSRYGDLNGLKTLINKLHKNNIGVLLDWVPSHFCKDEHGLFMFDGSPTYEYEAWWKANNEGWGTCNFDLGRPEVK
SFLFSNAMYWINEFHVDGLRVDAVSNMLYLDYGREYGEWEPNIYGGNGNLEAIAFLKELNTIIKKEGKGAITVAEESTSW
EGITKPVEEDGLGFDYKWNMGWMNDTLSYIELDPIYRKYHHNKMNFSMMYNYSEKFILPISHDEVVHGKKSLINKMWGDD
WKKYAGLRVYASFMMGHPGKKLMFMGCEFGQFVEWREWEELQWNVIEEFDIHRKTKEYFKALNKFYLENSSLWSLDYEEE
GFKWIDADNSEESVLSFIRIGKNKKEKLIFICNFTPEVYYDFKVGVPELGEYVEAFNSDALEFGGAGNIVGDSILKATEE
SFKDFDYSISVKVPPLGTLVLKVK

Sequences:

>Translated_664_residues
MGGKEMRNCKELKHEKNGNVTEKVGKNKGKSKKVSKDESLLSFDLFLEGKEHSAYKFMGAHFITENRKRGVRFTTWAPRA
SKIYVIGDFNNWELKEEYSMKKINERGIWSLFLPKLEEGIKYKFAVVNECGNNTVYKADPYAFKSELRPNTASVLTKIKS
FRWGDKRWLNKREKEGLDNKPMNIYELHLGSWKRKDGEFMTYEEISEVLVEYIKEMGYTHVEFMPINEHPLDASWGYQGV
GYYSVTSRYGDLNGLKTLINKLHKNNIGVLLDWVPSHFCKDEHGLFMFDGSPTYEYEAWWKANNEGWGTCNFDLGRPEVK
SFLFSNAMYWINEFHVDGLRVDAVSNMLYLDYGREYGEWEPNIYGGNGNLEAIAFLKELNTIIKKEGKGAITVAEESTSW
EGITKPVEEDGLGFDYKWNMGWMNDTLSYIELDPIYRKYHHNKMNFSMMYNYSEKFILPISHDEVVHGKKSLINKMWGDD
WKKYAGLRVYASFMMGHPGKKLMFMGCEFGQFVEWREWEELQWNVIEEFDIHRKTKEYFKALNKFYLENSSLWSLDYEEE
GFKWIDADNSEESVLSFIRIGKNKKEKLIFICNFTPEVYYDFKVGVPELGEYVEAFNSDALEFGGAGNIVGDSILKATEE
SFKDFDYSISVKVPPLGTLVLKVK
>Mature_663_residues
GGKEMRNCKELKHEKNGNVTEKVGKNKGKSKKVSKDESLLSFDLFLEGKEHSAYKFMGAHFITENRKRGVRFTTWAPRAS
KIYVIGDFNNWELKEEYSMKKINERGIWSLFLPKLEEGIKYKFAVVNECGNNTVYKADPYAFKSELRPNTASVLTKIKSF
RWGDKRWLNKREKEGLDNKPMNIYELHLGSWKRKDGEFMTYEEISEVLVEYIKEMGYTHVEFMPINEHPLDASWGYQGVG
YYSVTSRYGDLNGLKTLINKLHKNNIGVLLDWVPSHFCKDEHGLFMFDGSPTYEYEAWWKANNEGWGTCNFDLGRPEVKS
FLFSNAMYWINEFHVDGLRVDAVSNMLYLDYGREYGEWEPNIYGGNGNLEAIAFLKELNTIIKKEGKGAITVAEESTSWE
GITKPVEEDGLGFDYKWNMGWMNDTLSYIELDPIYRKYHHNKMNFSMMYNYSEKFILPISHDEVVHGKKSLINKMWGDDW
KKYAGLRVYASFMMGHPGKKLMFMGCEFGQFVEWREWEELQWNVIEEFDIHRKTKEYFKALNKFYLENSSLWSLDYEEEG
FKWIDADNSEESVLSFIRIGKNKKEKLIFICNFTPEVYYDFKVGVPELGEYVEAFNSDALEFGGAGNIVGDSILKATEES
FKDFDYSISVKVPPLGTLVLKVK

Specific function: Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position

COG id: COG0296

COG function: function code G; 1,4-alpha-glucan branching enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family

Homologues:

Organism=Homo sapiens, GI189458812, Length=717, Percent_Identity=25.2440725244073, Blast_Score=184, Evalue=3e-46,
Organism=Escherichia coli, GI1789839, Length=620, Percent_Identity=42.5806451612903, Blast_Score=530, Evalue=1e-152,
Organism=Caenorhabditis elegans, GI17554896, Length=652, Percent_Identity=28.2208588957055, Blast_Score=194, Evalue=1e-49,
Organism=Caenorhabditis elegans, GI32564391, Length=615, Percent_Identity=27.3170731707317, Blast_Score=167, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6320826, Length=673, Percent_Identity=27.3402674591382, Blast_Score=178, Evalue=2e-45,
Organism=Drosophila melanogaster, GI28573410, Length=674, Percent_Identity=27.0029673590504, Blast_Score=195, Evalue=9e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLGB2_CLOPE (Q8XK15)

Other databases:

- EMBL:   BA000016
- RefSeq:   NP_562504.1
- ProteinModelPortal:   Q8XK15
- SMR:   Q8XK15
- GeneID:   989898
- GenomeReviews:   BA000016_GR
- KEGG:   cpe:CPE1588
- NMPDR:   fig|195102.1.peg.1651
- HOGENOM:   HBG287139
- OMA:   PRIRANN
- PhylomeDB:   Q8XK15
- ProtClustDB:   PRK05402
- BioCyc:   CPER195102:CPE1588-MONOMER
- BRENDA:   2.4.1.18
- HAMAP:   MF_00685
- InterPro:   IPR006407
- InterPro:   IPR006048
- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783
- InterPro:   IPR014756
- Gene3D:   G3DSA:2.60.40.1180
- Gene3D:   G3DSA:3.20.20.80
- Gene3D:   G3DSA:2.60.40.10
- TIGRFAMs:   TIGR01515

Pfam domain/function: PF00128 Alpha-amylase; PF02806 Alpha-amylase_C; PF02922 CBM_48; SSF51445 Glyco_hydro_cat; SSF81296 Ig_E-set

EC number: =2.4.1.18

Molecular weight: Translated: 77486; Mature: 77355

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: NA

Important sites: ACT_SITE 237-237 ACT_SITE 272-272 ACT_SITE 277-277 ACT_SITE 340-340 ACT_SITE 342-342 ACT_SITE 395-395 ACT_SITE 462-462 ACT_SITE 463-463

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGGKEMRNCKELKHEKNGNVTEKVGKNKGKSKKVSKDESLLSFDLFLEGKEHSAYKFMGA
CCCHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCCCCCCEEEEEEEEECCCCCCEEEHHH
HFITENRKRGVRFTTWAPRASKIYVIGDFNNWELKEEYSMKKINERGIWSLFLPKLEEGI
HEEECCCCCCEEEEEECCCCCEEEEEECCCCCCCHHHHHHHHHCCCCCHHHHHHHHHCCC
KYKFAVVNECGNNTVYKADPYAFKSELRPNTASVLTKIKSFRWGDKRWLNKREKEGLDNK
EEEEEEEECCCCCCEEECCCHHHHHHCCCCHHHHHHHHHHCCCCCHHHCCHHHHCCCCCC
PMNIYELHLGSWKRKDGEFMTYEEISEVLVEYIKEMGYTHVEFMPINEHPLDASWGYQGV
CCEEEEEECCCCCCCCCCEECHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCC
GYYSVTSRYGDLNGLKTLINKLHKNNIGVLLDWVPSHFCKDEHGLFMFDGSPTYEYEAWW
EEEEEECCCCCHHHHHHHHHHHCCCCCCEEEECCCHHHCCCCCCEEEECCCCCEEEEEEE
KANNEGWGTCNFDLGRPEVKSFLFSNAMYWINEFHVDGLRVDAVSNMLYLDYGREYGEWE
EECCCCCEEEECCCCCHHHHHHHHHHHHEEHHEEECCCEEEEHHCCEEEEECCCCCCCCC
PNIYGGNGNLEAIAFLKELNTIIKKEGKGAITVAEESTSWEGITKPVEEDGLGFDYKWNM
CEEECCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCHHHCCCCEEEEECC
GWMNDTLSYIELDPIYRKYHHNKMNFSMMYNYSEKFILPISHDEVVHGKKSLINKMWGDD
CCCCCCCCEEEECHHHHHHCCCCCEEEEEEECCCEEEEEECCCHHHCCHHHHHHHHCCCH
WKKYAGLRVYASFMMGHPGKKLMFMGCEFGQFVEWREWEELQWNVIEEFDIHRKTKEYFK
HHHHCCHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHH
ALNKFYLENSSLWSLDYEEEGFKWIDADNSEESVLSFIRIGKNKKEKLIFICNFTPEVYY
HHHHHHCCCCCEEEECCCCCCCEEECCCCCHHHHHHHHHHCCCCCCEEEEEEECCCCEEE
DFKVGVPELGEYVEAFNSDALEFGGAGNIVGDSILKATEESFKDFDYSISVKVPPLGTLV
EEECCCHHHHHHHHHHCCCCEECCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCEEEE
LKVK
EEEC
>Mature Secondary Structure 
GGKEMRNCKELKHEKNGNVTEKVGKNKGKSKKVSKDESLLSFDLFLEGKEHSAYKFMGA
CCHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCCCCCCEEEEEEEEECCCCCCEEEHHH
HFITENRKRGVRFTTWAPRASKIYVIGDFNNWELKEEYSMKKINERGIWSLFLPKLEEGI
HEEECCCCCCEEEEEECCCCCEEEEEECCCCCCCHHHHHHHHHCCCCCHHHHHHHHHCCC
KYKFAVVNECGNNTVYKADPYAFKSELRPNTASVLTKIKSFRWGDKRWLNKREKEGLDNK
EEEEEEEECCCCCCEEECCCHHHHHHCCCCHHHHHHHHHHCCCCCHHHCCHHHHCCCCCC
PMNIYELHLGSWKRKDGEFMTYEEISEVLVEYIKEMGYTHVEFMPINEHPLDASWGYQGV
CCEEEEEECCCCCCCCCCEECHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCC
GYYSVTSRYGDLNGLKTLINKLHKNNIGVLLDWVPSHFCKDEHGLFMFDGSPTYEYEAWW
EEEEEECCCCCHHHHHHHHHHHCCCCCCEEEECCCHHHCCCCCCEEEECCCCCEEEEEEE
KANNEGWGTCNFDLGRPEVKSFLFSNAMYWINEFHVDGLRVDAVSNMLYLDYGREYGEWE
EECCCCCEEEECCCCCHHHHHHHHHHHHEEHHEEECCCEEEEHHCCEEEEECCCCCCCCC
PNIYGGNGNLEAIAFLKELNTIIKKEGKGAITVAEESTSWEGITKPVEEDGLGFDYKWNM
CEEECCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCHHHCCCCEEEEECC
GWMNDTLSYIELDPIYRKYHHNKMNFSMMYNYSEKFILPISHDEVVHGKKSLINKMWGDD
CCCCCCCCEEEECHHHHHHCCCCCEEEEEEECCCEEEEEECCCHHHCCHHHHHHHHCCCH
WKKYAGLRVYASFMMGHPGKKLMFMGCEFGQFVEWREWEELQWNVIEEFDIHRKTKEYFK
HHHHCCHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHH
ALNKFYLENSSLWSLDYEEEGFKWIDADNSEESVLSFIRIGKNKKEKLIFICNFTPEVYY
HHHHHHCCCCCEEEECCCCCCCEEECCCCCHHHHHHHHHHCCCCCCEEEEEEECCCCEEE
DFKVGVPELGEYVEAFNSDALEFGGAGNIVGDSILKATEESFKDFDYSISVKVPPLGTLV
EEECCCHHHHHHHHHHCCCCEECCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCEEEE
LKVK
EEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11792842