| Definition | Clostridium perfringens str. 13, complete genome. |
|---|---|
| Accession | NC_003366 |
| Length | 3,031,430 |
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The map label for this gene is gshAB
Identifier: 18310555
GI number: 18310555
Start: 1841356
End: 1843692
Strand: Reverse
Name: gshAB
Synonym: CPE1573
Alternate gene names: 18310555
Gene position: 1843692-1841356 (Counterclockwise)
Preceding gene: 18310556
Following gene: 18310553
Centisome position: 60.82
GC content: 27.98
Gene sequence:
>2337_bases ATGGTGAATTTAGATAAAGGTTTATTAAAAATTATAAAAGATGAAAGTTTAGAAGATTACTTTATAAAGGCTAATTTTGG TTTAGAAAAAGAAAATGTTAGGGTTACAGAAAGCGGAAACTTAGCTTTAACACCTCATCCAAAGGCCTTTGGAGATAGGG AAAAGAATGCATATATAAAAACAGATTTTTCTGAGAGTCAGTTAGAAATGGTAACACCTGTCTGTAATACTTTAGAGGAA GTTTATAGTTTTATATGTAATTTAAATAAGGTTGTATCTTTAGAGATTATGAAAAATGGAGAATTTTTATGGCCACAGAG TAATCCTCCTATATTACCAAGGGAAGAGGAGATTCCAATTGCTAAGCTTTCTAATAGAGAAGATGAATTATATAGAGAAA ATCTTAGTTATAAATACGGCAAAAAGAAACAAGTTATAAGTGGAATACATTATAATTTTTCATTTAAAGAGGAATTTATT AAGTTACTTTACAAAGAGCTTAAAGTGGAAAAGGATTTTAGAGAATTTAAAGATGATATCTACCTTAGAATGGCTAGAAA CTTTCAAAAATATCATTGGTTATTAATATACTTAACTGGAGCAAGTCCTGTTTTCCATGAAAGTTATATAGAGGAAATTA AAGAAGAGGGTGAAAAATTAGGAGAGGATTCTTATTATATAAAAGATGATACTTCTTTAAGAAATAGTTCTTATGGATAT AAAAATAAAAAGGACTATTATGTTTCATATAACAGTATAGAAGAATATGCTAGTGACATAAAGAATTTAGTTAAGGATAA GGAAATACAAAGTATAAAAGAATATTATAATCCTATAAGGTTAAAATCACTAGGAAGCGAAGATATGCTTGAAAGCTTAC TTCACAAGGGGATTGATTACTTAGAGGTAAGACTTTTAGACTTAGATCCTTTAAGTATTCAAGGAGTGAGTAAAGAAACA CTTTATCTATTGCATTTATTTATGATTTATACTTTATTAAAAGAGAACAAGGAAATAACATATAAAGATCAAGAAGAATT TTTCAAAAATCATGATATGGTTGCTTTAAAGGGTAGAAATGAAGAGGCCGTTATATATGAAAATGGAGTTCCTGTTTTAT TAAAAGACAAAGGAAGAGAAATACTAAGTGAAATGGATGAAATTGTAGAGATCTTATTTTCAAATAATGAGGAATTTAAA AATGTTATCAAAAGAGCATTAGAAAAAATTAATAATCCTCATGATACAATTTCAGAGAAACTTATTAAGGATATAAAAGA AGAGGGATATATTAATTTCCACATGAGATTAGCTAAGGAGTACTTAAATAACTTTAAAAATAAAGAATTTAATTTAGTTG GTTATGAAGATTTAGAATTATCAACACAAATATTAATATTAGATGCTATTAAAAGAGGAATAGAGTTTAATATTATGGAT AGATTAGAGAACTTTATTTCTCTTAGTGATGGAGAAAAGGTTGAATATGTTAAGCAAGCAACAAAAACTTCTAAGGATTC ATATATAACTGCCTTAATAATGGAAAATAAATTAGTTACTAAGGATATTTTAAGGGAAAATAATATAAGGGTTCCTAAGG GGAAAGATTATGACAATATAGATGAGGCAAAGAAAGATTTTAGATTATTTAAGGATGAGAAAATAGTTATAAAACCTAAG TCTACTAATTTTGGTTTAGGAATTAGTATATTTCCTGGAGAATATTCAAGGGAAGACTATGATAAAGCTGTTGAAATAGC TTTTAGAGAGGATAGTTCAATCCTTATAGAAGAATTTATGACAGGAAAGGAATATAGATTTCTTGTAATAGGGGAAGAGG TAGTAGGAATACTTCATAGAGAACCTGCTAATGTAATTGGTAATGGAGAAAGTACCATAGAAGAGCTTGTTTCTGAAAAA AATAAAGATCCATTAAGAGGAAAGGGATATAAGACTCCTTTAGAAAAAATAAAATTAGGAGAGATAGAAGAAATGTTTTT GAAAAATCAAGGACTAAGCTTTAAGTCTATTCCTAAAAATGGAGAAAAAATCTATTTAAGAGAAAACTCTAATATAAGCA CAGGAGGAGACAGCATAGACTTTACTGACAAAATACATCCTAGTTATAAAGAGGTGGCATTAAAGTCTGCTAAGGCTGTT AAAGCCCTTATATGCGGAGTAGATATGGTAATAGATAATATAGAGGAAGAGGCAAAGGAAAAAAATCATGGCATAATAGA ATTGAATTTTAACCCAGCAATACATATTCATTGTTTCCCTTATAAAGGAGAGAATAGAAAAGCTGGTGAAAAGATATTAG ATTTATTGTTTAATTAA
Upstream 100 bases:
>100_bases AAAATAGGTTAGTTCATAAATAATACATAAGTAGATGTTTTATTATAAATGGTTAAATAGAACTGGTTAGTATAGATAAG AGAATTCCAGGAGGAGAGAA
Downstream 100 bases:
>100_bases ATTAGATATAAAACTTGTAGAAATAAGATAATACTAAGTAAAAAGTAGATATAAAATAAGATTTTAATGGCTTTATCAAA ATATAAAAGAAAAACTTACA
Product: bifunctional glutamate--cysteine ligase/glutathione synthetase
Products: NA
Alternate protein names: Gamma-GCS-GS; GCS-GS; Glutamate--cysteine ligase; Gamma-ECS; GCS; Gamma-glutamylcysteine synthetase; Glutathione synthetase; GSH synthetase; GS; GSH-S; GSHase; Glutathione synthase
Number of amino acids: Translated: 778; Mature: 778
Protein sequence:
>778_residues MVNLDKGLLKIIKDESLEDYFIKANFGLEKENVRVTESGNLALTPHPKAFGDREKNAYIKTDFSESQLEMVTPVCNTLEE VYSFICNLNKVVSLEIMKNGEFLWPQSNPPILPREEEIPIAKLSNREDELYRENLSYKYGKKKQVISGIHYNFSFKEEFI KLLYKELKVEKDFREFKDDIYLRMARNFQKYHWLLIYLTGASPVFHESYIEEIKEEGEKLGEDSYYIKDDTSLRNSSYGY KNKKDYYVSYNSIEEYASDIKNLVKDKEIQSIKEYYNPIRLKSLGSEDMLESLLHKGIDYLEVRLLDLDPLSIQGVSKET LYLLHLFMIYTLLKENKEITYKDQEEFFKNHDMVALKGRNEEAVIYENGVPVLLKDKGREILSEMDEIVEILFSNNEEFK NVIKRALEKINNPHDTISEKLIKDIKEEGYINFHMRLAKEYLNNFKNKEFNLVGYEDLELSTQILILDAIKRGIEFNIMD RLENFISLSDGEKVEYVKQATKTSKDSYITALIMENKLVTKDILRENNIRVPKGKDYDNIDEAKKDFRLFKDEKIVIKPK STNFGLGISIFPGEYSREDYDKAVEIAFREDSSILIEEFMTGKEYRFLVIGEEVVGILHREPANVIGNGESTIEELVSEK NKDPLRGKGYKTPLEKIKLGEIEEMFLKNQGLSFKSIPKNGEKIYLRENSNISTGGDSIDFTDKIHPSYKEVALKSAKAV KALICGVDMVIDNIEEEAKEKNHGIIELNFNPAIHIHCFPYKGENRKAGEKILDLLFN
Sequences:
>Translated_778_residues MVNLDKGLLKIIKDESLEDYFIKANFGLEKENVRVTESGNLALTPHPKAFGDREKNAYIKTDFSESQLEMVTPVCNTLEE VYSFICNLNKVVSLEIMKNGEFLWPQSNPPILPREEEIPIAKLSNREDELYRENLSYKYGKKKQVISGIHYNFSFKEEFI KLLYKELKVEKDFREFKDDIYLRMARNFQKYHWLLIYLTGASPVFHESYIEEIKEEGEKLGEDSYYIKDDTSLRNSSYGY KNKKDYYVSYNSIEEYASDIKNLVKDKEIQSIKEYYNPIRLKSLGSEDMLESLLHKGIDYLEVRLLDLDPLSIQGVSKET LYLLHLFMIYTLLKENKEITYKDQEEFFKNHDMVALKGRNEEAVIYENGVPVLLKDKGREILSEMDEIVEILFSNNEEFK NVIKRALEKINNPHDTISEKLIKDIKEEGYINFHMRLAKEYLNNFKNKEFNLVGYEDLELSTQILILDAIKRGIEFNIMD RLENFISLSDGEKVEYVKQATKTSKDSYITALIMENKLVTKDILRENNIRVPKGKDYDNIDEAKKDFRLFKDEKIVIKPK STNFGLGISIFPGEYSREDYDKAVEIAFREDSSILIEEFMTGKEYRFLVIGEEVVGILHREPANVIGNGESTIEELVSEK NKDPLRGKGYKTPLEKIKLGEIEEMFLKNQGLSFKSIPKNGEKIYLRENSNISTGGDSIDFTDKIHPSYKEVALKSAKAV KALICGVDMVIDNIEEEAKEKNHGIIELNFNPAIHIHCFPYKGENRKAGEKILDLLFN >Mature_778_residues MVNLDKGLLKIIKDESLEDYFIKANFGLEKENVRVTESGNLALTPHPKAFGDREKNAYIKTDFSESQLEMVTPVCNTLEE VYSFICNLNKVVSLEIMKNGEFLWPQSNPPILPREEEIPIAKLSNREDELYRENLSYKYGKKKQVISGIHYNFSFKEEFI KLLYKELKVEKDFREFKDDIYLRMARNFQKYHWLLIYLTGASPVFHESYIEEIKEEGEKLGEDSYYIKDDTSLRNSSYGY KNKKDYYVSYNSIEEYASDIKNLVKDKEIQSIKEYYNPIRLKSLGSEDMLESLLHKGIDYLEVRLLDLDPLSIQGVSKET LYLLHLFMIYTLLKENKEITYKDQEEFFKNHDMVALKGRNEEAVIYENGVPVLLKDKGREILSEMDEIVEILFSNNEEFK NVIKRALEKINNPHDTISEKLIKDIKEEGYINFHMRLAKEYLNNFKNKEFNLVGYEDLELSTQILILDAIKRGIEFNIMD RLENFISLSDGEKVEYVKQATKTSKDSYITALIMENKLVTKDILRENNIRVPKGKDYDNIDEAKKDFRLFKDEKIVIKPK STNFGLGISIFPGEYSREDYDKAVEIAFREDSSILIEEFMTGKEYRFLVIGEEVVGILHREPANVIGNGESTIEELVSEK NKDPLRGKGYKTPLEKIKLGEIEEMFLKNQGLSFKSIPKNGEKIYLRENSNISTGGDSIDFTDKIHPSYKEVALKSAKAV KALICGVDMVIDNIEEEAKEKNHGIIELNFNPAIHIHCFPYKGENRKAGEKILDLLFN
Specific function: Synthesizes glutathione from L-glutamate and L-cysteine via gamma-L-glutamyl-L-cysteine
COG id: COG2918
COG function: function code H; Gamma-glutamylcysteine synthetase
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Contains 1 ATP-grasp domain
Homologues:
Organism=Escherichia coli, GI1789044, Length=467, Percent_Identity=28.9079229122056, Blast_Score=196, Evalue=6e-51,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): GSHAB_CLOPE (Q8XK30)
Other databases:
- EMBL: BA000016 - RefSeq: NP_562489.1 - ProteinModelPortal: Q8XK30 - SMR: Q8XK30 - GeneID: 989883 - GenomeReviews: BA000016_GR - KEGG: cpe:CPE1573 - NMPDR: fig|195102.1.peg.1636 - HOGENOM: HBG296652 - OMA: PKSTNYG - PhylomeDB: Q8XK30 - ProtClustDB: PRK02471 - BioCyc: CPER195102:CPE1573-MONOMER - BRENDA: 6.3.2.2 - BRENDA: 6.3.2.3 - HAMAP: MF_00782 - InterPro: IPR011761 - InterPro: IPR013815 - InterPro: IPR013816 - InterPro: IPR007370 - InterPro: IPR006335 - InterPro: IPR020561 - Gene3D: G3DSA:3.30.1490.20 - Gene3D: G3DSA:3.30.470.20 - TIGRFAMs: TIGR01435
Pfam domain/function: PF01071 GARS_A; PF04262 Glu_cys_ligase
EC number: =6.3.2.2; =6.3.2.3
Molecular weight: Translated: 90464; Mature: 90464
Theoretical pI: Translated: 5.06; Mature: 5.06
Prosite motif: PS50975 ATP_GRASP
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.5 %Cys (Translated Protein) 1.8 %Met (Translated Protein) 2.3 %Cys+Met (Translated Protein) 0.5 %Cys (Mature Protein) 1.8 %Met (Mature Protein) 2.3 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MVNLDKGLLKIIKDESLEDYFIKANFGLEKENVRVTESGNLALTPHPKAFGDREKNAYIK CCCCHHHHHHHHHCCCCCCEEEEECCCCCCCCEEEEECCCEEECCCCCCCCCCCCCCEEE TDFSESQLEMVTPVCNTLEEVYSFICNLNKVVSLEIMKNGEFLWPQSNPPILPREEEIPI ECCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCEECCCCCCCCCCCCCCCCH AKLSNREDELYRENLSYKYGKKKQVISGIHYNFSFKEEFIKLLYKELKVEKDFREFKDDI HHHCCCHHHHHHHCCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH YLRMARNFQKYHWLLIYLTGASPVFHESYIEEIKEEGEKLGEDSYYIKDDTSLRNSSYGY HHHHHHCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCC KNKKDYYVSYNSIEEYASDIKNLVKDKEIQSIKEYYNPIRLKSLGSEDMLESLLHKGIDY CCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCHHHHHHHHHCCCCE LEVRLLDLDPLSIQGVSKETLYLLHLFMIYTLLKENKEITYKDQEEFFKNHDMVALKGRN EEEEEEECCCCEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCEEEEECCC EEAVIYENGVPVLLKDKGREILSEMDEIVEILFSNNEEFKNVIKRALEKINNPHDTISEK CCEEEEECCCEEEECCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHH LIKDIKEEGYINFHMRLAKEYLNNFKNKEFNLVGYEDLELSTQILILDAIKRGIEFNIMD HHHHHHHCCEEEHHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHEEEHHHHCCCCEEHHH RLENFISLSDGEKVEYVKQATKTSKDSYITALIMENKLVTKDILRENNIRVPKGKDYDNI HHHHHHCCCCCCHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHCCCCCCCCCCCCCCH DEAKKDFRLFKDEKIVIKPKSTNFGLGISIFPGEYSREDYDKAVEIAFREDSSILIEEFM HHHHHHHHHHCCCEEEEEECCCCCCEEEEECCCCCCCHHHHHHEEEEEECCCHHHHHHHH TGKEYRFLVIGEEVVGILHREPANVIGNGESTIEELVSEKNKDPLRGKGYKTPLEKIKLG CCCCEEEEEECHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHCCC EIEEMFLKNQGLSFKSIPKNGEKIYLRENSNISTGGDSIDFTDKIHPSYKEVALKSAKAV HHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH KALICGVDMVIDNIEEEAKEKNHGIIELNFNPAIHIHCFPYKGENRKAGEKILDLLFN HHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCEEEEEEEECCCCCCHHHHHHHHHHCC >Mature Secondary Structure MVNLDKGLLKIIKDESLEDYFIKANFGLEKENVRVTESGNLALTPHPKAFGDREKNAYIK CCCCHHHHHHHHHCCCCCCEEEEECCCCCCCCEEEEECCCEEECCCCCCCCCCCCCCEEE TDFSESQLEMVTPVCNTLEEVYSFICNLNKVVSLEIMKNGEFLWPQSNPPILPREEEIPI ECCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCEECCCCCCCCCCCCCCCCH AKLSNREDELYRENLSYKYGKKKQVISGIHYNFSFKEEFIKLLYKELKVEKDFREFKDDI HHHCCCHHHHHHHCCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH YLRMARNFQKYHWLLIYLTGASPVFHESYIEEIKEEGEKLGEDSYYIKDDTSLRNSSYGY HHHHHHCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCC KNKKDYYVSYNSIEEYASDIKNLVKDKEIQSIKEYYNPIRLKSLGSEDMLESLLHKGIDY CCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCHHHHHHHHHCCCCE LEVRLLDLDPLSIQGVSKETLYLLHLFMIYTLLKENKEITYKDQEEFFKNHDMVALKGRN EEEEEEECCCCEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCEEEEECCC EEAVIYENGVPVLLKDKGREILSEMDEIVEILFSNNEEFKNVIKRALEKINNPHDTISEK CCEEEEECCCEEEECCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHH LIKDIKEEGYINFHMRLAKEYLNNFKNKEFNLVGYEDLELSTQILILDAIKRGIEFNIMD HHHHHHHCCEEEHHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHEEEHHHHCCCCEEHHH RLENFISLSDGEKVEYVKQATKTSKDSYITALIMENKLVTKDILRENNIRVPKGKDYDNI HHHHHHCCCCCCHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHCCCCCCCCCCCCCCH DEAKKDFRLFKDEKIVIKPKSTNFGLGISIFPGEYSREDYDKAVEIAFREDSSILIEEFM HHHHHHHHHHCCCEEEEEECCCCCCEEEEECCCCCCCHHHHHHEEEEEECCCHHHHHHHH TGKEYRFLVIGEEVVGILHREPANVIGNGESTIEELVSEKNKDPLRGKGYKTPLEKIKLG CCCCEEEEEECHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHCCC EIEEMFLKNQGLSFKSIPKNGEKIYLRENSNISTGGDSIDFTDKIHPSYKEVALKSAKAV HHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH KALICGVDMVIDNIEEEAKEKNHGIIELNFNPAIHIHCFPYKGENRKAGEKILDLLFN HHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCEEEEEEEECCCCCCHHHHHHHHHHCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 11792842