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The map label for this gene is ilvE [H]

Identifier: 18310502

GI number: 18310502

Start: 1778114

End: 1779139

Strand: Direct

Name: ilvE [H]

Synonym: CPE1520

Alternate gene names: 18310502

Gene position: 1778114-1779139 (Clockwise)

Preceding gene: 18310488

Following gene: 18310506

Centisome position: 58.66

GC content: 33.82

Gene sequence:

>1026_bases
ATGGATAAAAAGACAGCTATTGATTGGAATAATCTAGGCTTCTCTTATATGAAAACAGATTATCGTTACATATCTCACTA
TAAAGATGGTAAATGGGATGAAGGAAAATTAGTTACAGACAACAAATTAAGCATAAGTGAAGCTTCAACTGCCCTTCACT
ATGGCCAACAATGTTTTGAAGGTTTAAAAGCTTATAGAACAAAGGATGGAAAGATTCAACTTTTTAGAGTAGATGAAAAT
GCTAAGAGAATGAATAAATCATGTGATAAACTTTTAATGCCTGAAATACCAGTTGAAAAATTCATAGATGCTTGTATGCA
AGTTGTCAAGGCTAATGAAAGATTTGTACCTCCATACGGTACTGGTGCAACTCTTTATATAAGACCTTTCATGATAGGTG
TTGGTGATAATATAGGTGTTAAATCTGCTCCTGAATTTATATTTTCAGTATTCTGCCTTCCAGTTGGTGCTTATTTTAAA
GGTGGAATGAAGCCTGTAAACTTTATGATTGCAGATTATGATAGAGCTGCTCCTAAAGGAACTGGTGCCGCTAAAGTTGG
TGGAAATTACGCAGCAAGCTTAAAGGCTCATGAAATAGCTGCAAAAAAAGGATTTGCTGATTGTATATATTTAGACCCAG
CAACTCACACTAAAATTGAGGAAGTTGGAGCTGCAAACTTCTTTGGAATAACAAAGAAAGGTGAGTTTGTTACTCCATAT
TCAGAATCAATTTTACCAAGTATAACAAAATACTCTTTAATGCAAATAGCTAAAGATTATTTAAAAATGCCTGTATCAGA
AAGAGATGTTTTAATAGATAACTTAGATGAATTCGCTGAGGCTGGCGCTTGTGGTACAGCCGCTGTAATAACTCCAATAG
GAGGAATAGAATATAAGAATAAACTTCATGTTTTCCATAGCGAAACTGAAGTTGGTCCTATTACTAAAAAACTTTATGAT
CTTTTATCTGGAATGCAATTTGGAGATGTAGAAGCTCCTGAAGGATGGATATTTGAAGTTAAATAA

Upstream 100 bases:

>100_bases
AAAATAATTTTAATTTGCAATTTTTAAATTTTAATCTTTCATTTTTTAAAATAAAATGATAAAATTAAAAAGTATTAAAT
TTTGATAGGAGTGTTAAACT

Downstream 100 bases:

>100_bases
TACATAAAAAAGAGAGTGAGTAGTAAACTCCTCTCTTTTTCTCTTAATTAAGAAAACTTTCTTTTATTTCTCTTAGTAAT
TCTTTATAATTATCACCAGA

Product: branched-chain amino acid aminotransferase

Products: NA

Alternate protein names: BCAT [H]

Number of amino acids: Translated: 341; Mature: 341

Protein sequence:

>341_residues
MDKKTAIDWNNLGFSYMKTDYRYISHYKDGKWDEGKLVTDNKLSISEASTALHYGQQCFEGLKAYRTKDGKIQLFRVDEN
AKRMNKSCDKLLMPEIPVEKFIDACMQVVKANERFVPPYGTGATLYIRPFMIGVGDNIGVKSAPEFIFSVFCLPVGAYFK
GGMKPVNFMIADYDRAAPKGTGAAKVGGNYAASLKAHEIAAKKGFADCIYLDPATHTKIEEVGAANFFGITKKGEFVTPY
SESILPSITKYSLMQIAKDYLKMPVSERDVLIDNLDEFAEAGACGTAAVITPIGGIEYKNKLHVFHSETEVGPITKKLYD
LLSGMQFGDVEAPEGWIFEVK

Sequences:

>Translated_341_residues
MDKKTAIDWNNLGFSYMKTDYRYISHYKDGKWDEGKLVTDNKLSISEASTALHYGQQCFEGLKAYRTKDGKIQLFRVDEN
AKRMNKSCDKLLMPEIPVEKFIDACMQVVKANERFVPPYGTGATLYIRPFMIGVGDNIGVKSAPEFIFSVFCLPVGAYFK
GGMKPVNFMIADYDRAAPKGTGAAKVGGNYAASLKAHEIAAKKGFADCIYLDPATHTKIEEVGAANFFGITKKGEFVTPY
SESILPSITKYSLMQIAKDYLKMPVSERDVLIDNLDEFAEAGACGTAAVITPIGGIEYKNKLHVFHSETEVGPITKKLYD
LLSGMQFGDVEAPEGWIFEVK
>Mature_341_residues
MDKKTAIDWNNLGFSYMKTDYRYISHYKDGKWDEGKLVTDNKLSISEASTALHYGQQCFEGLKAYRTKDGKIQLFRVDEN
AKRMNKSCDKLLMPEIPVEKFIDACMQVVKANERFVPPYGTGATLYIRPFMIGVGDNIGVKSAPEFIFSVFCLPVGAYFK
GGMKPVNFMIADYDRAAPKGTGAAKVGGNYAASLKAHEIAAKKGFADCIYLDPATHTKIEEVGAANFFGITKKGEFVTPY
SESILPSITKYSLMQIAKDYLKMPVSERDVLIDNLDEFAEAGACGTAAVITPIGGIEYKNKLHVFHSETEVGPITKKLYD
LLSGMQFGDVEAPEGWIFEVK

Specific function: Acts on leucine, isoleucine and valine [H]

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI296010906, Length=341, Percent_Identity=34.0175953079179, Blast_Score=167, Evalue=2e-41,
Organism=Homo sapiens, GI296010904, Length=341, Percent_Identity=34.0175953079179, Blast_Score=166, Evalue=2e-41,
Organism=Homo sapiens, GI38176287, Length=341, Percent_Identity=34.0175953079179, Blast_Score=166, Evalue=2e-41,
Organism=Homo sapiens, GI50658084, Length=348, Percent_Identity=31.3218390804598, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI258614015, Length=303, Percent_Identity=32.013201320132, Blast_Score=130, Evalue=1e-30,
Organism=Homo sapiens, GI296010902, Length=341, Percent_Identity=29.9120234604106, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI296010900, Length=341, Percent_Identity=29.9120234604106, Blast_Score=120, Evalue=2e-27,
Organism=Escherichia coli, GI48994963, Length=318, Percent_Identity=28.9308176100629, Blast_Score=123, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17568601, Length=359, Percent_Identity=29.2479108635098, Blast_Score=162, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI17565728, Length=345, Percent_Identity=30.4347826086957, Blast_Score=117, Evalue=6e-27,
Organism=Saccharomyces cerevisiae, GI6322608, Length=342, Percent_Identity=33.6257309941521, Blast_Score=174, Evalue=2e-44,
Organism=Saccharomyces cerevisiae, GI6322002, Length=306, Percent_Identity=35.6209150326797, Blast_Score=168, Evalue=1e-42,
Organism=Drosophila melanogaster, GI24641779, Length=338, Percent_Identity=33.1360946745562, Blast_Score=173, Evalue=2e-43,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005786 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.42 [H]

Molecular weight: Translated: 37790; Mature: 37790

Theoretical pI: Translated: 7.08; Mature: 7.08

Prosite motif: PS00770 AA_TRANSFER_CLASS_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKKTAIDWNNLGFSYMKTDYRYISHYKDGKWDEGKLVTDNKLSISEASTALHYGQQCFE
CCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHH
GLKAYRTKDGKIQLFRVDENAKRMNKSCDKLLMPEIPVEKFIDACMQVVKANERFVPPYG
HHHHHCCCCCCEEEEEECCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCC
TGATLYIRPFMIGVGDNIGVKSAPEFIFSVFCLPVGAYFKGGMKPVNFMIADYDRAAPKG
CCCEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCC
TGAAKVGGNYAASLKAHEIAAKKGFADCIYLDPATHTKIEEVGAANFFGITKKGEFVTPY
CCCEECCCCEEEHHHHHHHHHHCCCCEEEEECCCCCCHHHHHCCCCEEEECCCCCEECCC
SESILPSITKYSLMQIAKDYLKMPVSERDVLIDNLDEFAEAGACGTAAVITPIGGIEYKN
HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCHHHHHHCCCCCCEEEEECCCCCCCCC
KLHVFHSETEVGPITKKLYDLLSGMQFGDVEAPEGWIFEVK
EEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEC
>Mature Secondary Structure
MDKKTAIDWNNLGFSYMKTDYRYISHYKDGKWDEGKLVTDNKLSISEASTALHYGQQCFE
CCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHH
GLKAYRTKDGKIQLFRVDENAKRMNKSCDKLLMPEIPVEKFIDACMQVVKANERFVPPYG
HHHHHCCCCCCEEEEEECCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCC
TGATLYIRPFMIGVGDNIGVKSAPEFIFSVFCLPVGAYFKGGMKPVNFMIADYDRAAPKG
CCCEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCC
TGAAKVGGNYAASLKAHEIAAKKGFADCIYLDPATHTKIEEVGAANFFGITKKGEFVTPY
CCCEECCCCEEEHHHHHHHHHHCCCCEEEEECCCCCCHHHHHCCCCEEEECCCCCEECCC
SESILPSITKYSLMQIAKDYLKMPVSERDVLIDNLDEFAEAGACGTAAVITPIGGIEYKN
HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCHHHHHHCCCCCCEEEEECCCCCCCCC
KLHVFHSETEVGPITKKLYDLLSGMQFGDVEAPEGWIFEVK
EEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]