Click here to switch to the map view.

The map label for this gene is tig

Identifier: 18310376

GI number: 18310376

Start: 1645096

End: 1646382

Strand: Reverse

Name: tig

Synonym: CPE1394

Alternate gene names: 18310376

Gene position: 1646382-1645096 (Counterclockwise)

Preceding gene: 18310377

Following gene: 18310375

Centisome position: 54.31

GC content: 31.7

Gene sequence:

>1287_bases
ATGGAAGCTAAAATGAACAAAATAGACACAAATGTTGTAGAATTAGAAATTAAAGTTGATGCTAAGGACTTTAACGAGGC
GTTAAAAAAGTCTTATAATAAAAACTCAAAGAAGTTTAACATACCAGGATTCAGAAAAGGAAAAGTTCCAATGAACATGG
TTAAAAAATTCTACGGAGTTGAAGTTTTATTTGATGATGCAATAAACGCTTGTATAGATAAAACTTACGGAGTTGCTTTA
GAAGAAAACAACGTAAGACCAGTTGATTATCCACAAATCGAAGTAGTAGAAGTTGGAGAAGGAAAAGACTTAGTTTACAA
AGCTAAAGTTACTACTTACCCAGAAGTTACTTTAGGAGATTACAAAGGATTAGAAGTTGAAGAAGTATCTTACGAAGTAA
AAGAAGAAGACATAGAAAAACAATTAGCTGATATGCAAGCAAGAAATGCTAGAGTTGAAACTAAAGAAGAAGGAACAGTT
GAAAATGGAAACATAGCTGTTATAGACTTCAAAGGATTCATAGATGACGTAGCATTCGAAGGTGGAGAAGGAAAAGATTA
TCCATTAGAAATAGGTTCAGGAAGCTTCATAGATAACTTTGAAGAGCAATTAGTTGGATTAAAAGTTGGAGAAAGCAAAG
ATGTTAACGTAACATTCCCAGAAGCTTATGGAAAAGAAGACTTAAACGGAAAACCAGCTAAATTCGAAGTAACTGTAAAA
GAAATAAAAGTTAAAGAATTACCAGCTTTAGATGATGAGTTTGCTAAAGAAGTTTCAGAATTTGATACTTTAGAAGAATT
AAAAGCTGACTTAAAAGAAAAAGCTGTAAAAGCTAACGAATTAAGAGCTAAAAGAGAAATGGAAGAAAAAGTTATAAACG
CTGTAGTTGATAACGCAAAAGTAGAAATTCCAGAAGCTATGATAAACAGAGAAGTTGAAAACATGGTAAGAGACTTAGAA
ATGAGATTAGGACAACAAGGACTAAGCTTAGAGCAATACTATGAGTTCACTGGAAGCACTGAAGATAAGATGAAATCTTA
CATGAAAGAAAATGCTGAAAGAAAAGTAAAAACTGATTTAGTTATGAGTGCTGTAACTGAAGCAGAAGCTATAGAAGCTA
CTGAAGAAGAATTAAAAGCAAAAGCAGAAGAAGTTGCTAAAATGTACTCAAACGGAGCAGAAACTGAGAAAATGGTTGAT
CTATTATTAAATGCTCAAAGAGCTGCTTTAGAGTTAGATGTTAAGAGAGAAAAAACTTTAAAAATGTTATTTGAATCTTT
AAAATAA

Upstream 100 bases:

>100_bases
TAAAAGAAATATTTGATAAAAAAAGAAATTATATGTTATAATTGCCTAGGTTAATTCATAAAAGATGAATTTAAAAGGCT
ATTTACAGGAGGAATATACA

Downstream 100 bases:

>100_bases
TAAATAAATAAACTTTCAGATAATTGCCAGGAAGTATTTCTTGGCAATTATTTTAAAATTAAAAGTTTGTTTAGAAAAAG
ACTAATTAACAAATATTTAT

Product: trigger factor

Products: peptidylproline (w =0) [C]

Alternate protein names: TF

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MEAKMNKIDTNVVELEIKVDAKDFNEALKKSYNKNSKKFNIPGFRKGKVPMNMVKKFYGVEVLFDDAINACIDKTYGVAL
EENNVRPVDYPQIEVVEVGEGKDLVYKAKVTTYPEVTLGDYKGLEVEEVSYEVKEEDIEKQLADMQARNARVETKEEGTV
ENGNIAVIDFKGFIDDVAFEGGEGKDYPLEIGSGSFIDNFEEQLVGLKVGESKDVNVTFPEAYGKEDLNGKPAKFEVTVK
EIKVKELPALDDEFAKEVSEFDTLEELKADLKEKAVKANELRAKREMEEKVINAVVDNAKVEIPEAMINREVENMVRDLE
MRLGQQGLSLEQYYEFTGSTEDKMKSYMKENAERKVKTDLVMSAVTEAEAIEATEEELKAKAEEVAKMYSNGAETEKMVD
LLLNAQRAALELDVKREKTLKMLFESLK

Sequences:

>Translated_428_residues
MEAKMNKIDTNVVELEIKVDAKDFNEALKKSYNKNSKKFNIPGFRKGKVPMNMVKKFYGVEVLFDDAINACIDKTYGVAL
EENNVRPVDYPQIEVVEVGEGKDLVYKAKVTTYPEVTLGDYKGLEVEEVSYEVKEEDIEKQLADMQARNARVETKEEGTV
ENGNIAVIDFKGFIDDVAFEGGEGKDYPLEIGSGSFIDNFEEQLVGLKVGESKDVNVTFPEAYGKEDLNGKPAKFEVTVK
EIKVKELPALDDEFAKEVSEFDTLEELKADLKEKAVKANELRAKREMEEKVINAVVDNAKVEIPEAMINREVENMVRDLE
MRLGQQGLSLEQYYEFTGSTEDKMKSYMKENAERKVKTDLVMSAVTEAEAIEATEEELKAKAEEVAKMYSNGAETEKMVD
LLLNAQRAALELDVKREKTLKMLFESLK
>Mature_428_residues
MEAKMNKIDTNVVELEIKVDAKDFNEALKKSYNKNSKKFNIPGFRKGKVPMNMVKKFYGVEVLFDDAINACIDKTYGVAL
EENNVRPVDYPQIEVVEVGEGKDLVYKAKVTTYPEVTLGDYKGLEVEEVSYEVKEEDIEKQLADMQARNARVETKEEGTV
ENGNIAVIDFKGFIDDVAFEGGEGKDYPLEIGSGSFIDNFEEQLVGLKVGESKDVNVTFPEAYGKEDLNGKPAKFEVTVK
EIKVKELPALDDEFAKEVSEFDTLEELKADLKEKAVKANELRAKREMEEKVINAVVDNAKVEIPEAMINREVENMVRDLE
MRLGQQGLSLEQYYEFTGSTEDKMKSYMKENAERKVKTDLVMSAVTEAEAIEATEEELKAKAEEVAKMYSNGAETEKMVD
LLLNAQRAALELDVKREKTLKMLFESLK

Specific function: Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation

COG id: COG0544

COG function: function code O; FKBP-type peptidyl-prolyl cis-trans isomerase (trigger factor)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase FKBP-type domain

Homologues:

Organism=Escherichia coli, GI1786640, Length=403, Percent_Identity=30.7692307692308, Blast_Score=172, Evalue=5e-44,

Paralogues:

None

Copy number: 1070 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 500 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 340 Molecules/Cell In: Growth-Phase, Mini

Swissprot (AC and ID): TIG_CLOP1 (Q0TQK1)

Other databases:

- EMBL:   CP000246
- RefSeq:   YP_696089.1
- ProteinModelPortal:   Q0TQK1
- STRING:   Q0TQK1
- GeneID:   4202975
- GenomeReviews:   CP000246_GR
- KEGG:   cpf:CPF_1648
- TIGR:   CPF_1648
- eggNOG:   COG0544
- HOGENOM:   HBG635105
- OMA:   RFIPGFE
- ProtClustDB:   PRK01490
- BioCyc:   CPER195103:CPF_1648-MONOMER
- HAMAP:   MF_00303
- InterPro:   IPR001179
- InterPro:   IPR005215
- InterPro:   IPR008880
- InterPro:   IPR008881
- Gene3D:   G3DSA:1.10.3120.10
- Gene3D:   G3DSA:3.30.70.1050
- PIRSF:   PIRSF003095
- TIGRFAMs:   TIGR00115

Pfam domain/function: PF00254 FKBP_C; PF05698 Trigger_C; PF05697 Trigger_N; SSF109998 Trigger_fac_C_bac; SSF102735 Trigger_fac_ribosome-bd_bac

EC number: NA

Molecular weight: Translated: 48361; Mature: 48361

Theoretical pI: Translated: 4.38; Mature: 4.38

Prosite motif: PS50059 FKBP_PPIASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEAKMNKIDTNVVELEIKVDAKDFNEALKKSYNKNSKKFNIPGFRKGKVPMNMVKKFYGV
CCCCCCCCCCEEEEEEEEECHHHHHHHHHHHHCCCCCEECCCCCCCCCCCHHHHHHHHCH
EVLFDDAINACIDKTYGVALEENNVRPVDYPQIEVVEVGEGKDLVYKAKVTTYPEVTLGD
HHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCEEEEEECCCCCEEEEEEECCCCCEEECC
YKGLEVEEVSYEVKEEDIEKQLADMQARNARVETKEEGTVENGNIAVIDFKGFIDDVAFE
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEHHHHHHHHCC
GGEGKDYPLEIGSGSFIDNFEEQLVGLKVGESKDVNVTFPEAYGKEDLNGKPAKFEVTVK
CCCCCCCCEECCCCCHHHHHHHHHHCEECCCCCCCEEECCHHCCCCCCCCCCCEEEEEHH
EIKVKELPALDDEFAKEVSEFDTLEELKADLKEKAVKANELRAKREMEEKVINAVVDNAK
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
VEIPEAMINREVENMVRDLEMRLGQQGLSLEQYYEFTGSTEDKMKSYMKENAERKVKTDL
EECCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
VMSAVTEAEAIEATEEELKAKAEEVAKMYSNGAETEKMVDLLLNAQRAALELDVKREKTL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHEEEHHHHHHHH
KMLFESLK
HHHHHHCC
>Mature Secondary Structure
MEAKMNKIDTNVVELEIKVDAKDFNEALKKSYNKNSKKFNIPGFRKGKVPMNMVKKFYGV
CCCCCCCCCCEEEEEEEEECHHHHHHHHHHHHCCCCCEECCCCCCCCCCCHHHHHHHHCH
EVLFDDAINACIDKTYGVALEENNVRPVDYPQIEVVEVGEGKDLVYKAKVTTYPEVTLGD
HHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCEEEEEECCCCCEEEEEEECCCCCEEECC
YKGLEVEEVSYEVKEEDIEKQLADMQARNARVETKEEGTVENGNIAVIDFKGFIDDVAFE
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEHHHHHHHHCC
GGEGKDYPLEIGSGSFIDNFEEQLVGLKVGESKDVNVTFPEAYGKEDLNGKPAKFEVTVK
CCCCCCCCEECCCCCHHHHHHHHHHCEECCCCCCCEEECCHHCCCCCCCCCCCEEEEEHH
EIKVKELPALDDEFAKEVSEFDTLEELKADLKEKAVKANELRAKREMEEKVINAVVDNAK
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
VEIPEAMINREVENMVRDLEMRLGQQGLSLEQYYEFTGSTEDKMKSYMKENAERKVKTDL
EECCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
VMSAVTEAEAIEATEEELKAKAEEVAKMYSNGAETEKMVDLLLNAQRAALELDVKREKTL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHEEEHHHHHHHH
KMLFESLK
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: peptidylproline (w =180) [C]

Specific reaction: peptidylproline (w =180) = peptidylproline (w =0) [C]

General reaction: Isomerases; cis-trans-Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA