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The map label for this gene is sigL [H]

Identifier: 18310288

GI number: 18310288

Start: 1546057

End: 1547442

Strand: Reverse

Name: sigL [H]

Synonym: CPE1306

Alternate gene names: 18310288

Gene position: 1547442-1546057 (Counterclockwise)

Preceding gene: 18310289

Following gene: 18310287

Centisome position: 51.05

GC content: 28.35

Gene sequence:

>1386_bases
ATGTTAATGGATTTTAATTTGAATTTAACTCAAGAGCAGAAGCTTATTATGACCCAGCAAATGCAACTTTCAATAAAACT
TTTGCAAATGTCGACATATGATTTAAGAGAATATATTGAAAAAGAATTTTCAGAAAATCCTGTATTAGAAGCTCAATATG
AAGATACTAAAGAGGTATCAAAAGAGCAAGATAGATTAGAATATAAAGAGTTAGTAAAATATTTAGAGTCAGATAACTAT
GGCTCTCAAAGTTATGGTGAATATGATCAAGAAGGAATATCACCTTTTACTTTCATAAGTAAGCCAGAATCTTTAACAGA
TTATTTAGAAGGGCAAATATTAGAATTACCAATAGATGAATATATGAGAAGTGTATGTAGTTATATGGTCGAGTGTTTAG
ATCAAAAGGGATATTTAGATATAAAAAAAGAAGAATTAATGGGGGAGCTAGATTGTTCTGAAGAGACTTTTAATAGGGCT
TTAATAGTTCTTCATAATTTAGAACCTGCCGGTATAGGGGCAAGAGATTTAAAGGAATGCCTAGAAATTCAGTTAGAAAG
AAAAGGTGAAAATGACCCTATAGTTAAAGAGATTATAGATAATCATTTAGACGATTTAGCAGATAATAAATATCAAGTTA
TTGCAAAGGATTTAGGAATTACTCCTAAAAAAGCACAAGATTATGGAGATTTAATAAAAACTTTAGAACCAAAACCATCA
AGAGGCTTTTACACTGGTGATGAAGTGGGTTTTATAATTCCTGATGCAGAAATACGAAAGATAGATGGAGAATTCCTTAT
ATTAATGAATGATGGAGTTTTACCTATGCTTTCAGTTAATCCTTTATATAAAGATATATTAAAAGATAGTACTAATGATA
AAGAGGCTACAGAGTATGTAAAGGAAAAAATAGAAAAGGCTATGTTTTTAATTAAAAGTATAGAGCAAAGAAAAAGTACT
TTATACAAAGTTCTTGAAAAAATACTTGAAAAGCAAAAGGATTATTTTGAAAAGGGAGAGAAATATTTAAAGCCTATGAC
TTTAAAAGAAATAGCTGAACAATTAGAAATGCATGAATCAACTATTTCAAGAGCTATAAGGGATAAGTATATTTTAACTT
CTATGGGAACAATAAAAATAAAGAATCTCTTTGTAAACTCAATAAGTAATAAAGAAAAAAGTGACGGAGAAGAAGATGTT
ACAGTTATAAATATAAAAAAAGCTTTAGAAGAAGTAATTAAGGAAGAAAATAAAGCAAAGCCATTATCAGATCAAACCAT
AAGTGAAATTCTAAAAGAAAAAGGAATGGTTATTTCAAGAAGAACTGTAGCAAAATACAGAGAAGAGTTAGGCATAAAGT
CATCTAGCAAGAGAAAAAGATTTTAA

Upstream 100 bases:

>100_bases
TAAATTATTGAAAAATAATTTTATTTGATGGATAATTATATAAGAAAGGTTAAAAGATAAAAGTAAATAGGTTAGAATTA
GATAATTTTAGGTGATATAT

Downstream 100 bases:

>100_bases
AAGAGAAGAATTTCTATCTTGTGGTAAATGAGGTTATAAAGTGCTTATTTTGAGTGAAAAATATAGAGAGACTGGGTAAT
CTCTCTATATTTTTTTATAT

Product: RNA polymerase factor sigma-54

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 461; Mature: 461

Protein sequence:

>461_residues
MLMDFNLNLTQEQKLIMTQQMQLSIKLLQMSTYDLREYIEKEFSENPVLEAQYEDTKEVSKEQDRLEYKELVKYLESDNY
GSQSYGEYDQEGISPFTFISKPESLTDYLEGQILELPIDEYMRSVCSYMVECLDQKGYLDIKKEELMGELDCSEETFNRA
LIVLHNLEPAGIGARDLKECLEIQLERKGENDPIVKEIIDNHLDDLADNKYQVIAKDLGITPKKAQDYGDLIKTLEPKPS
RGFYTGDEVGFIIPDAEIRKIDGEFLILMNDGVLPMLSVNPLYKDILKDSTNDKEATEYVKEKIEKAMFLIKSIEQRKST
LYKVLEKILEKQKDYFEKGEKYLKPMTLKEIAEQLEMHESTISRAIRDKYILTSMGTIKIKNLFVNSISNKEKSDGEEDV
TVINIKKALEEVIKEENKAKPLSDQTISEILKEKGMVISRRTVAKYREELGIKSSSKRKRF

Sequences:

>Translated_461_residues
MLMDFNLNLTQEQKLIMTQQMQLSIKLLQMSTYDLREYIEKEFSENPVLEAQYEDTKEVSKEQDRLEYKELVKYLESDNY
GSQSYGEYDQEGISPFTFISKPESLTDYLEGQILELPIDEYMRSVCSYMVECLDQKGYLDIKKEELMGELDCSEETFNRA
LIVLHNLEPAGIGARDLKECLEIQLERKGENDPIVKEIIDNHLDDLADNKYQVIAKDLGITPKKAQDYGDLIKTLEPKPS
RGFYTGDEVGFIIPDAEIRKIDGEFLILMNDGVLPMLSVNPLYKDILKDSTNDKEATEYVKEKIEKAMFLIKSIEQRKST
LYKVLEKILEKQKDYFEKGEKYLKPMTLKEIAEQLEMHESTISRAIRDKYILTSMGTIKIKNLFVNSISNKEKSDGEEDV
TVINIKKALEEVIKEENKAKPLSDQTISEILKEKGMVISRRTVAKYREELGIKSSSKRKRF
>Mature_461_residues
MLMDFNLNLTQEQKLIMTQQMQLSIKLLQMSTYDLREYIEKEFSENPVLEAQYEDTKEVSKEQDRLEYKELVKYLESDNY
GSQSYGEYDQEGISPFTFISKPESLTDYLEGQILELPIDEYMRSVCSYMVECLDQKGYLDIKKEELMGELDCSEETFNRA
LIVLHNLEPAGIGARDLKECLEIQLERKGENDPIVKEIIDNHLDDLADNKYQVIAKDLGITPKKAQDYGDLIKTLEPKPS
RGFYTGDEVGFIIPDAEIRKIDGEFLILMNDGVLPMLSVNPLYKDILKDSTNDKEATEYVKEKIEKAMFLIKSIEQRKST
LYKVLEKILEKQKDYFEKGEKYLKPMTLKEIAEQLEMHESTISRAIRDKYILTSMGTIKIKNLFVNSISNKEKSDGEEDV
TVINIKKALEEVIKEENKAKPLSDQTISEILKEKGMVISRRTVAKYREELGIKSSSKRKRF

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released [H]

COG id: COG1508

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma54 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-54 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789594, Length=482, Percent_Identity=32.5726141078838, Blast_Score=265, Evalue=4e-72,

Paralogues:

None

Copy number: 70 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000394
- InterPro:   IPR007046
- InterPro:   IPR007634 [H]

Pfam domain/function: PF00309 Sigma54_AID; PF04963 Sigma54_CBD; PF04552 Sigma54_DBD [H]

EC number: NA

Molecular weight: Translated: 53444; Mature: 53444

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: PS00717 SIGMA54_1 ; PS00718 SIGMA54_2 ; PS50044 SIGMA54_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLMDFNLNLTQEQKLIMTQQMQLSIKLLQMSTYDLREYIEKEFSENPVLEAQYEDTKEVS
CEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCHHHHHH
KEQDRLEYKELVKYLESDNYGSQSYGEYDQEGISPFTFISKPESLTDYLEGQILELPIDE
HHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCCEECCCCHHHHHHHCCCEEECCHHH
YMRSVCSYMVECLDQKGYLDIKKEELMGELDCSEETFNRALIVLHNLEPAGIGARDLKEC
HHHHHHHHHHHHHCCCCCCEECHHHHHHHCCCCHHHHCCEEEEEECCCCCCCCHHHHHHH
LEIQLERKGENDPIVKEIIDNHLDDLADNKYQVIAKDLGITPKKAQDYGDLIKTLEPKPS
HHHHHHCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCHHHHHHHHHHHCCCCCC
RGFYTGDEVGFIIPDAEIRKIDGEFLILMNDGVLPMLSVNPLYKDILKDSTNDKEATEYV
CCCCCCCCCEEEECCHHHEEECCCEEEEECCCCCCEEECCHHHHHHHCCCCCCHHHHHHH
KEKIEKAMFLIKSIEQRKSTLYKVLEKILEKQKDYFEKGEKYLKPMTLKEIAEQLEMHES
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
TISRAIRDKYILTSMGTIKIKNLFVNSISNKEKSDGEEDVTVINIKKALEEVIKEENKAK
HHHHHHHHHHHHCCCCCEEEHHHHHHHHCCCCCCCCCCCEEEEEHHHHHHHHHHHHCCCC
PLSDQTISEILKEKGMVISRRTVAKYREELGIKSSSKRKRF
CCCHHHHHHHHHHCCCEEEHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MLMDFNLNLTQEQKLIMTQQMQLSIKLLQMSTYDLREYIEKEFSENPVLEAQYEDTKEVS
CEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCHHHHHH
KEQDRLEYKELVKYLESDNYGSQSYGEYDQEGISPFTFISKPESLTDYLEGQILELPIDE
HHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCCEECCCCHHHHHHHCCCEEECCHHH
YMRSVCSYMVECLDQKGYLDIKKEELMGELDCSEETFNRALIVLHNLEPAGIGARDLKEC
HHHHHHHHHHHHHCCCCCCEECHHHHHHHCCCCHHHHCCEEEEEECCCCCCCCHHHHHHH
LEIQLERKGENDPIVKEIIDNHLDDLADNKYQVIAKDLGITPKKAQDYGDLIKTLEPKPS
HHHHHHCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCHHHHHHHHHHHCCCCCC
RGFYTGDEVGFIIPDAEIRKIDGEFLILMNDGVLPMLSVNPLYKDILKDSTNDKEATEYV
CCCCCCCCCEEEECCHHHEEECCCEEEEECCCCCCEEECCHHHHHHHCCCCCCHHHHHHH
KEKIEKAMFLIKSIEQRKSTLYKVLEKILEKQKDYFEKGEKYLKPMTLKEIAEQLEMHES
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
TISRAIRDKYILTSMGTIKIKNLFVNSISNKEKSDGEEDVTVINIKKALEEVIKEENKAK
HHHHHHHHHHHHCCCCCEEEHHHHHHHHCCCCCCCCCCCEEEEEHHHHHHHHHHHHCCCC
PLSDQTISEILKEKGMVISRRTVAKYREELGIKSSSKRKRF
CCCHHHHHHHHHHCCCEEEHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8550525 [H]