Click here to switch to the map view.

The map label for this gene is cbiK [H]

Identifier: 18310210

GI number: 18310210

Start: 1430168

End: 1431010

Strand: Reverse

Name: cbiK [H]

Synonym: CPE1228

Alternate gene names: 18310210

Gene position: 1431010-1430168 (Counterclockwise)

Preceding gene: 18310212

Following gene: 18310209

Centisome position: 47.21

GC content: 28.94

Gene sequence:

>843_bases
ATGAAAAAAGCTATATTAGTAGTAAGTTTTGGAACAAGTCATTTAGACGCGTTAAATAATTCTATAAAGAAAATAGAAGA
TTCAATACAGGAAAACTTTAAAGAATATGATGTTTTTAGAAGTTTTACATCACATATGATAGTAAGAAAACTTAAAGAAA
AACATAATATGGAAGTATTATTTCCAGAGGATGCTTTAAAGAAATTAGGAGAGGAAGGATATGAGGAAGTTATAGTTCAA
CCACTTCATATTATTCCAGGAGAAGAGTTTGATTATATAAAGCATATGGTAAAGGAATATGAACCATGCTTTAAAAAGAT
AGAAATAGGAAGACCAATATTCCATTATGAAGGTTTTGAATCAGGTCCAGATGATTATTCATATTTCGTAGATACAATGA
AAGATTTAATAGAAAAGAATTCTCCATGTGTATTTGTTGGACATGGTAGTGCTCATCCATCAAATGCTGTTTATGGATGT
TTACAATCTGTTTTAATAGATAAGGACTATGAAGATGTTTTTGTAGGAACTGTAGAAGGGTATCCTAATTTTTCAAATGT
CTTAAAGAAAGCTAAGAAGAAAGATATAAAAGAAATAACAATAATTCCTTTAATGGTTGTAGCTGGAGATCATGCTAAAA
ATGATATGGCTTCAGATGAAGAAGATTCATGGAAGAGCAGATTTGAAAGTGAAGGAATAAAGGCTAATGTTATTTTAAAA
GGCTTAGGAGAATACGAAGAGTTTGGAAATTTATACATAGATAGAATTAATGATGTTATTAATGGTACATATAAAGGACT
TGGAGAAACTAAAAAGAAGAAACATAGAAGGGAAATGAAATAA

Upstream 100 bases:

>100_bases
ACTTATTTGTTCATAATTTTGAGGAATAATTACTTTGATTTGTAAGCATCTGTTCTTTGTAGCACAGATGCTTTTTATTT
TTAAATTTTAGAGGTGAAAT

Downstream 100 bases:

>100_bases
TGGATTACATGAAAAGCCCTATGGGAATTGAAAAAAGAAGTTTTGAAATTATAGGTTCTGAAATGCATGAACATAATTTT
TCAGAAGAGGAATTACAAAT

Product: CbiK protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 280; Mature: 280

Protein sequence:

>280_residues
MKKAILVVSFGTSHLDALNNSIKKIEDSIQENFKEYDVFRSFTSHMIVRKLKEKHNMEVLFPEDALKKLGEEGYEEVIVQ
PLHIIPGEEFDYIKHMVKEYEPCFKKIEIGRPIFHYEGFESGPDDYSYFVDTMKDLIEKNSPCVFVGHGSAHPSNAVYGC
LQSVLIDKDYEDVFVGTVEGYPNFSNVLKKAKKKDIKEITIIPLMVVAGDHAKNDMASDEEDSWKSRFESEGIKANVILK
GLGEYEEFGNLYIDRINDVINGTYKGLGETKKKKHRREMK

Sequences:

>Translated_280_residues
MKKAILVVSFGTSHLDALNNSIKKIEDSIQENFKEYDVFRSFTSHMIVRKLKEKHNMEVLFPEDALKKLGEEGYEEVIVQ
PLHIIPGEEFDYIKHMVKEYEPCFKKIEIGRPIFHYEGFESGPDDYSYFVDTMKDLIEKNSPCVFVGHGSAHPSNAVYGC
LQSVLIDKDYEDVFVGTVEGYPNFSNVLKKAKKKDIKEITIIPLMVVAGDHAKNDMASDEEDSWKSRFESEGIKANVILK
GLGEYEEFGNLYIDRINDVINGTYKGLGETKKKKHRREMK
>Mature_280_residues
MKKAILVVSFGTSHLDALNNSIKKIEDSIQENFKEYDVFRSFTSHMIVRKLKEKHNMEVLFPEDALKKLGEEGYEEVIVQ
PLHIIPGEEFDYIKHMVKEYEPCFKKIEIGRPIFHYEGFESGPDDYSYFVDTMKDLIEKNSPCVFVGHGSAHPSNAVYGC
LQSVLIDKDYEDVFVGTVEGYPNFSNVLKKAKKKDIKEITIIPLMVVAGDHAKNDMASDEEDSWKSRFESEGIKANVILK
GLGEYEEFGNLYIDRINDVINGTYKGLGETKKKKHRREMK

Specific function: Chelates cobalt to the cobalamin precursor as part of the anaerobic pathway to cobalamin biosynthesis [H]

COG id: COG4822

COG function: function code H; Cobalamin biosynthesis protein CbiK, Co2+ chelatase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CbiK family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010388 [H]

Pfam domain/function: PF06180 CbiK [H]

EC number: =4.99.1.3 [H]

Molecular weight: Translated: 32134; Mature: 32134

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: PS00761 SPASE_I_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKAILVVSFGTSHLDALNNSIKKIEDSIQENFKEYDVFRSFTSHMIVRKLKEKHNMEVL
CCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEE
FPEDALKKLGEEGYEEVIVQPLHIIPGEEFDYIKHMVKEYEPCFKKIEIGRPIFHYEGFE
ECHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCC
SGPDDYSYFVDTMKDLIEKNSPCVFVGHGSAHPSNAVYGCLQSVLIDKDYEDVFVGTVEG
CCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCC
YPNFSNVLKKAKKKDIKEITIIPLMVVAGDHAKNDMASDEEDSWKSRFESEGIKANVILK
CCCHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCHHHHHHHHHHCCCEEEHHHH
GLGEYEEFGNLYIDRINDVINGTYKGLGETKKKKHRREMK
CCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCC
>Mature Secondary Structure
MKKAILVVSFGTSHLDALNNSIKKIEDSIQENFKEYDVFRSFTSHMIVRKLKEKHNMEVL
CCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEE
FPEDALKKLGEEGYEEVIVQPLHIIPGEEFDYIKHMVKEYEPCFKKIEIGRPIFHYEGFE
ECHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCC
SGPDDYSYFVDTMKDLIEKNSPCVFVGHGSAHPSNAVYGCLQSVLIDKDYEDVFVGTVEG
CCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCC
YPNFSNVLKKAKKKDIKEITIIPLMVVAGDHAKNDMASDEEDSWKSRFESEGIKANVILK
CCCHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCHHHHHHHHHHCCCEEEHHHH
GLGEYEEFGNLYIDRINDVINGTYKGLGETKKKKHRREMK
CCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8501034; 11677609; 9150215; 10451360 [H]