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The map label for this gene is cbiF [H]

Identifier: 18310204

GI number: 18310204

Start: 1425704

End: 1426459

Strand: Reverse

Name: cbiF [H]

Synonym: CPE1222

Alternate gene names: 18310204

Gene position: 1426459-1425704 (Counterclockwise)

Preceding gene: 18310205

Following gene: 18310203

Centisome position: 47.06

GC content: 31.61

Gene sequence:

>756_bases
ATGTTATATTTTATAGGTGCAGGTCCAGGAGCTGTTGATTTAATAACTGTTAGAGGAAGAAATTTATTAGAAAAAGCAGA
TGTTGTAATATATGCAGGATCTTTAGTAAGCAAAGAACATTTAGATGTTTGTAAAGAAGGAGTTAAAATATATAACTCAG
CTTCAATGACTTTAGAAGAGGTAGTTGAAGTTATAAAAGAAAATGATGGTGAAGATAAAGTAATAGTAAGACTTCATACT
GGAGATCCATCAATATATGGAGCTATTAAGGAACAAATGGATGAACTAGACAAGTTCAATATACAATATGAGGTTGTACC
AGGAGTAAGTTCATTTACAGCAGCAGCTTCAGCCATAAAGAAAGAGTTTACTCTTCCATCAGTAACTCAAACTGTTATAT
TAACTAGAGTTGAAGGAAGAACACCAGTACCAGAGACAGAGGATTTAGAAAAGTTAGCAGGAATAGGAGCTTCTATGGCA
TTATTCCTTTCTATATCAATGATTGATAAGGTAGTTGAAAAATTAAAAAGAGGATATGGAAGAGAAGATGTTCCTATAGC
TGTAATTTCTAGAGCAACATGGGATGATCAAGAAATAATCATAGGAACATTAGAAAATATATCAGAAAAGGTTAAGGAAG
CTGGTATAAATAAATGTGCTCAAATATTAGTTGGAGATTTCATTGACTGTAATTATGAGAAGAGTCTTCTTTATGATAAG
CATTTTACTCATATGTTCAGAAAAGGAATTGAATAA

Upstream 100 bases:

>100_bases
AAATCTTAAATCATAGAGATTACATGTCTTTAATACTAGCAAGTAAATAGAGAAAAATTATAAATAAAAAATCGTATTTT
ATTAGGAGGCAAAGGATAAA

Downstream 100 bases:

>100_bases
ATATGATTGGAATAATAAGTGTTACTGAAAAGGGAGACTTTATAGGGGATAAGCTTAAGGATTTTTTTGAAGAAAATAAT
ATTAGTTTCAAAGGAATTTA

Product: precorrin-4 C11-methyltransferase

Products: S-adenosyl-L-homocysteine; Precorrin 5

Alternate protein names: Cobalt-precorrin-3 methylase [H]

Number of amino acids: Translated: 251; Mature: 251

Protein sequence:

>251_residues
MLYFIGAGPGAVDLITVRGRNLLEKADVVIYAGSLVSKEHLDVCKEGVKIYNSASMTLEEVVEVIKENDGEDKVIVRLHT
GDPSIYGAIKEQMDELDKFNIQYEVVPGVSSFTAAASAIKKEFTLPSVTQTVILTRVEGRTPVPETEDLEKLAGIGASMA
LFLSISMIDKVVEKLKRGYGREDVPIAVISRATWDDQEIIIGTLENISEKVKEAGINKCAQILVGDFIDCNYEKSLLYDK
HFTHMFRKGIE

Sequences:

>Translated_251_residues
MLYFIGAGPGAVDLITVRGRNLLEKADVVIYAGSLVSKEHLDVCKEGVKIYNSASMTLEEVVEVIKENDGEDKVIVRLHT
GDPSIYGAIKEQMDELDKFNIQYEVVPGVSSFTAAASAIKKEFTLPSVTQTVILTRVEGRTPVPETEDLEKLAGIGASMA
LFLSISMIDKVVEKLKRGYGREDVPIAVISRATWDDQEIIIGTLENISEKVKEAGINKCAQILVGDFIDCNYEKSLLYDK
HFTHMFRKGIE
>Mature_251_residues
MLYFIGAGPGAVDLITVRGRNLLEKADVVIYAGSLVSKEHLDVCKEGVKIYNSASMTLEEVVEVIKENDGEDKVIVRLHT
GDPSIYGAIKEQMDELDKFNIQYEVVPGVSSFTAAASAIKKEFTLPSVTQTVILTRVEGRTPVPETEDLEKLAGIGASMA
LFLSISMIDKVVEKLKRGYGREDVPIAVISRATWDDQEIIIGTLENISEKVKEAGINKCAQILVGDFIDCNYEKSLLYDK
HFTHMFRKGIE

Specific function: Catalyzes the methylation of C-11 in cobalt-precorrin-4 to form cobalt-precorrin-5 [H]

COG id: COG2875

COG function: function code H; Precorrin-4 methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=231, Percent_Identity=25.1082251082251, Blast_Score=80, Evalue=9e-17,
Organism=Saccharomyces cerevisiae, GI6322922, Length=242, Percent_Identity=25.6198347107438, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006362
- InterPro:   IPR003043 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: 2.1.1.133

Molecular weight: Translated: 27731; Mature: 27731

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: PS00839 SUMT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLYFIGAGPGAVDLITVRGRNLLEKADVVIYAGSLVSKEHLDVCKEGVKIYNSASMTLEE
CEEEEECCCCCEEEEEECCCCHHHHCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCCHHH
VVEVIKENDGEDKVIVRLHTGDPSIYGAIKEQMDELDKFNIQYEVVPGVSSFTAAASAIK
HHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHH
KEFTLPSVTQTVILTRVEGRTPVPETEDLEKLAGIGASMALFLSISMIDKVVEKLKRGYG
HHCCCCHHHHHHHEEEECCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCC
REDVPIAVISRATWDDQEIIIGTLENISEKVKEAGINKCAQILVGDFIDCNYEKSLLYDK
CCCCCEEEEECCCCCCCHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
HFTHMFRKGIE
HHHHHHHHCCC
>Mature Secondary Structure
MLYFIGAGPGAVDLITVRGRNLLEKADVVIYAGSLVSKEHLDVCKEGVKIYNSASMTLEE
CEEEEECCCCCEEEEEECCCCHHHHCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCCHHH
VVEVIKENDGEDKVIVRLHTGDPSIYGAIKEQMDELDKFNIQYEVVPGVSSFTAAASAIK
HHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHH
KEFTLPSVTQTVILTRVEGRTPVPETEDLEKLAGIGASMALFLSISMIDKVVEKLKRGYG
HHCCCCHHHHHHHEEEECCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCC
REDVPIAVISRATWDDQEIIIGTLENISEKVKEAGINKCAQILVGDFIDCNYEKSLLYDK
CCCCCEEEEECCCCCCCHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
HFTHMFRKGIE
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-adenosyl-L-methionine; Precorrin 4

Specific reaction: S-adenosyl-L-methionine + precorrin-4 = S-adenosyl-L-homocysteine + precorrin-5

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]