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The map label for this gene is pcrA [H]

Identifier: 18310188

GI number: 18310188

Start: 1411020

End: 1413014

Strand: Reverse

Name: pcrA [H]

Synonym: CPE1206

Alternate gene names: 18310188

Gene position: 1413014-1411020 (Counterclockwise)

Preceding gene: 18310190

Following gene: 18310187

Centisome position: 46.61

GC content: 26.17

Gene sequence:

>1995_bases
ATGTCTAATTTAGATAAATATCAAAATGAAGCTGTAAGAGCAAGGAGTAAAAGTGTCCTTGTTATAGCTCCACCAGGAGC
AGGAAAGACCACAGTAATATTAAATAGAATTAAATATCTTTTAGAAGAAAGAAAGGTTAAAGGAATACATGTTATAGTAA
TAACTTTTACTAAGGCGGCAGCAGAAAATATGAAAAGTAGATTTAAAGAAATGCATAAGGAAGGCGTTATTCCATTCTTT
GGAACCTTTCATGGACTTTTTTATAAAATTTTACTTAGGAATAAGGATGAAATAAGGCTTATAGAATCTAAGGATTCTTA
TAATATTATAAGGAAAGTATTATCCTCCTATATAGAAGAGGTAAGTGATGAAAAAATAAAAGAGGTATTAAATAATATAT
CTAGAAAGAAAGTTTCTTCTAAGGATTTAGAAATATCAATGACTTATGATATTTTTAATAAATGCTATGAAGCATATGAA
ACTTTTAAAAATGAGAGAGGACTTTTAGATTTTGATGATTTACAAATAAAAGCAGTAACTCTTTTAAAAGAAAATCCTCA
TATATTAGGATATTATAGAAATTTAATAAAATATATATTAGTTGATGAGTTTCAAGATTGTGATGAAGTTCAATTAGAAT
TTTTAAGACTTATAAATAATGAAGTTTTTTGTGTTGGAGATGAGGATCAAAGTATATATTCCTTTAGAGGAGCAACTCCT
AAATGTATGATAAACTTTGAGGAGAACTTTGAAGGTAGTGAGAAGGTTTATTTAAAATATAATTATAGAAGCTTAAAAAA
TATTGTGGAGGTATCTAAGGAGGTTATAAAGCATAATAAAGAGAGATACCAAAAAGAAATAATAGCTTTTAATGAAAATC
AAGGGAAAATAGAGCTTAAAAAGCCCTTTAATGAGTCTGAGGAAGCAAATATAATTGCTTTAAAAATAGAAGAAAGCAAA
AGGTTAGGAAATAAATATTCTGATAATGCTATTTTGTATAGAACAAATATGGAGAGCAGAAGTTTTATTGATGCCTTTAT
TAGAAAAAAGATTCCCTTTAAGCTTTTAGATAAAGAGTATAATTTCTTTAATCATTTTATATGTAGAGATTTAATTGCGT
ATTTAAGACTATCAATAGATCCATTTAATAAAGAAGATTTTAATAAAATAATAAACAAACCCTTTAGATATGTTTCAAAA
AGCGCCATTTATGGAGTGATGAATTCTAAGAAGAAAGAAAATGTTTTTGATATATTAATGAATGTGGAAGGCGTACATCC
ATTTCAAATGAGAAAGTTTGAGGAGCTAAAAAGTGACATAGCTTATTTAAATAAACTTTCCTTAAATAGTGCTATTGATT
TTATATTAAGTGATTTAGAGTATAGTCAGTATATAAGAGAGTATTCCTCTAAGTTTAAGCAAAATCAAGAGGATTTATTA
GATATTGTAGAAGAATTTAAGAGTTCAGCTATGGAATTTAAATCTATAATCACTTTTCTTTCTCACATAGAAAGCGTTGA
AGAAAATTTAGAAAACTTAAATTCTAAAGGTAATATAGACTCTGTTATATTAAGCACCATGCATGGGGTAAAGGGAATGC
AGTTTAATGATGTTCACATTGTAAATATAGTTGATGAAACAATACCACATAAAAACAGTATGGAAAATTTAGAAGAGGAG
AGAAGACTTTTTTATGTGGGTATAACTAGAGCCATAAATAATTTATATCTTTATGCACCTAAAAGTGTAAGAGGTAAATT
TAAGGAGCCATCTATATTTTTAAATAATACAAATCTAGATAGCCAATGTAATATAACTGATTATGGATTAAAGGAAGGAG
ATGTCATAAGACATAATTATTTTGGCATAGGTGAAATTTTAAAAATAGAGAAGGATACTATTTCAATTAAATTTATTCAA
GGAGAAAATAAAAAATTTTCTTTAAGAACTTTGATTGAAAATAATTTAATAGAAAAAATAATTTGTGAAGTTTAA

Upstream 100 bases:

>100_bases
TAGAATTATTACAATATATATGTTAATAAATATTATGGTTTACTAGAGTAATTTTAGTCAATAATTAAAATAAATTTTAT
TAAACATTAGGAGAATTATA

Downstream 100 bases:

>100_bases
ATGTGTAGTAACAAAGGAAATTAGAGGATTTAAAGAAAAATTTTCTTTGTTACTATAATTTTCAACATAATACACTTAAA
TAATATACTCGTAAATGAGT

Product: ATP-dependent helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 664; Mature: 663

Protein sequence:

>664_residues
MSNLDKYQNEAVRARSKSVLVIAPPGAGKTTVILNRIKYLLEERKVKGIHVIVITFTKAAAENMKSRFKEMHKEGVIPFF
GTFHGLFYKILLRNKDEIRLIESKDSYNIIRKVLSSYIEEVSDEKIKEVLNNISRKKVSSKDLEISMTYDIFNKCYEAYE
TFKNERGLLDFDDLQIKAVTLLKENPHILGYYRNLIKYILVDEFQDCDEVQLEFLRLINNEVFCVGDEDQSIYSFRGATP
KCMINFEENFEGSEKVYLKYNYRSLKNIVEVSKEVIKHNKERYQKEIIAFNENQGKIELKKPFNESEEANIIALKIEESK
RLGNKYSDNAILYRTNMESRSFIDAFIRKKIPFKLLDKEYNFFNHFICRDLIAYLRLSIDPFNKEDFNKIINKPFRYVSK
SAIYGVMNSKKKENVFDILMNVEGVHPFQMRKFEELKSDIAYLNKLSLNSAIDFILSDLEYSQYIREYSSKFKQNQEDLL
DIVEEFKSSAMEFKSIITFLSHIESVEENLENLNSKGNIDSVILSTMHGVKGMQFNDVHIVNIVDETIPHKNSMENLEEE
RRLFYVGITRAINNLYLYAPKSVRGKFKEPSIFLNNTNLDSQCNITDYGLKEGDVIRHNYFGIGEILKIEKDTISIKFIQ
GENKKFSLRTLIENNLIEKIICEV

Sequences:

>Translated_664_residues
MSNLDKYQNEAVRARSKSVLVIAPPGAGKTTVILNRIKYLLEERKVKGIHVIVITFTKAAAENMKSRFKEMHKEGVIPFF
GTFHGLFYKILLRNKDEIRLIESKDSYNIIRKVLSSYIEEVSDEKIKEVLNNISRKKVSSKDLEISMTYDIFNKCYEAYE
TFKNERGLLDFDDLQIKAVTLLKENPHILGYYRNLIKYILVDEFQDCDEVQLEFLRLINNEVFCVGDEDQSIYSFRGATP
KCMINFEENFEGSEKVYLKYNYRSLKNIVEVSKEVIKHNKERYQKEIIAFNENQGKIELKKPFNESEEANIIALKIEESK
RLGNKYSDNAILYRTNMESRSFIDAFIRKKIPFKLLDKEYNFFNHFICRDLIAYLRLSIDPFNKEDFNKIINKPFRYVSK
SAIYGVMNSKKKENVFDILMNVEGVHPFQMRKFEELKSDIAYLNKLSLNSAIDFILSDLEYSQYIREYSSKFKQNQEDLL
DIVEEFKSSAMEFKSIITFLSHIESVEENLENLNSKGNIDSVILSTMHGVKGMQFNDVHIVNIVDETIPHKNSMENLEEE
RRLFYVGITRAINNLYLYAPKSVRGKFKEPSIFLNNTNLDSQCNITDYGLKEGDVIRHNYFGIGEILKIEKDTISIKFIQ
GENKKFSLRTLIENNLIEKIICEV
>Mature_663_residues
SNLDKYQNEAVRARSKSVLVIAPPGAGKTTVILNRIKYLLEERKVKGIHVIVITFTKAAAENMKSRFKEMHKEGVIPFFG
TFHGLFYKILLRNKDEIRLIESKDSYNIIRKVLSSYIEEVSDEKIKEVLNNISRKKVSSKDLEISMTYDIFNKCYEAYET
FKNERGLLDFDDLQIKAVTLLKENPHILGYYRNLIKYILVDEFQDCDEVQLEFLRLINNEVFCVGDEDQSIYSFRGATPK
CMINFEENFEGSEKVYLKYNYRSLKNIVEVSKEVIKHNKERYQKEIIAFNENQGKIELKKPFNESEEANIIALKIEESKR
LGNKYSDNAILYRTNMESRSFIDAFIRKKIPFKLLDKEYNFFNHFICRDLIAYLRLSIDPFNKEDFNKIINKPFRYVSKS
AIYGVMNSKKKENVFDILMNVEGVHPFQMRKFEELKSDIAYLNKLSLNSAIDFILSDLEYSQYIREYSSKFKQNQEDLLD
IVEEFKSSAMEFKSIITFLSHIESVEENLENLNSKGNIDSVILSTMHGVKGMQFNDVHIVNIVDETIPHKNSMENLEEER
RLFYVGITRAINNLYLYAPKSVRGKFKEPSIFLNNTNLDSQCNITDYGLKEGDVIRHNYFGIGEILKIEKDTISIKFIQG
ENKKFSLRTLIENNLIEKIICEV

Specific function: Essential helicase [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=711, Percent_Identity=27.2855133614627, Blast_Score=259, Evalue=5e-70,
Organism=Escherichia coli, GI48994965, Length=634, Percent_Identity=28.7066246056782, Blast_Score=223, Evalue=4e-59,
Organism=Saccharomyces cerevisiae, GI6322369, Length=481, Percent_Identity=27.4428274428274, Blast_Score=114, Evalue=5e-26,
Organism=Saccharomyces cerevisiae, GI6324477, Length=644, Percent_Identity=23.1366459627329, Blast_Score=92, Evalue=2e-19,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005751
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 77750; Mature: 77619

Theoretical pI: Translated: 7.34; Mature: 7.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNLDKYQNEAVRARSKSVLVIAPPGAGKTTVILNRIKYLLEERKVKGIHVIVITFTKAA
CCCHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEECHHH
AENMKSRFKEMHKEGVIPFFGTFHGLFYKILLRNKDEIRLIESKDSYNIIRKVLSSYIEE
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHH
VSDEKIKEVLNNISRKKVSSKDLEISMTYDIFNKCYEAYETFKNERGLLDFDDLQIKAVT
HHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEE
LLKENPHILGYYRNLIKYILVDEFQDCDEVQLEFLRLINNEVFCVGDEDQSIYSFRGATP
EECCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCEEEEECCCCCEEEECCCCC
KCMINFEENFEGSEKVYLKYNYRSLKNIVEVSKEVIKHNKERYQKEIIAFNENQGKIELK
EEEEEECCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCEEEEE
KPFNESEEANIIALKIEESKRLGNKYSDNAILYRTNMESRSFIDAFIRKKIPFKLLDKEY
CCCCCCCCCCEEEEEEECHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCHHHHHHHH
NFFNHFICRDLIAYLRLSIDPFNKEDFNKIINKPFRYVSKSAIYGVMNSKKKENVFDILM
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
NVEGVHPFQMRKFEELKSDIAYLNKLSLNSAIDFILSDLEYSQYIREYSSKFKQNQEDLL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
DIVEEFKSSAMEFKSIITFLSHIESVEENLENLNSKGNIDSVILSTMHGVKGMQFNDVHI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCEECCEEE
VNIVDETIPHKNSMENLEEERRLFYVGITRAINNLYLYAPKSVRGKFKEPSIFLNNTNLD
EEEHHHHCCCCCCHHHHHHHHHEEEEEHHHHHCCEEEECCHHHCCCCCCCEEEEECCCCC
SQCNITDYGLKEGDVIRHNYFGIGEILKIEKDTISIKFIQGENKKFSLRTLIENNLIEKI
CCCCCEECCCCCCCEEEECCCCCCHHEEEECCEEEEEEEECCCCCCHHHHHHHHHHHHHH
ICEV
HHCC
>Mature Secondary Structure 
SNLDKYQNEAVRARSKSVLVIAPPGAGKTTVILNRIKYLLEERKVKGIHVIVITFTKAA
CCHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEECHHH
AENMKSRFKEMHKEGVIPFFGTFHGLFYKILLRNKDEIRLIESKDSYNIIRKVLSSYIEE
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHH
VSDEKIKEVLNNISRKKVSSKDLEISMTYDIFNKCYEAYETFKNERGLLDFDDLQIKAVT
HHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEE
LLKENPHILGYYRNLIKYILVDEFQDCDEVQLEFLRLINNEVFCVGDEDQSIYSFRGATP
EECCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCEEEEECCCCCEEEECCCCC
KCMINFEENFEGSEKVYLKYNYRSLKNIVEVSKEVIKHNKERYQKEIIAFNENQGKIELK
EEEEEECCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCEEEEE
KPFNESEEANIIALKIEESKRLGNKYSDNAILYRTNMESRSFIDAFIRKKIPFKLLDKEY
CCCCCCCCCCEEEEEEECHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCHHHHHHHH
NFFNHFICRDLIAYLRLSIDPFNKEDFNKIINKPFRYVSKSAIYGVMNSKKKENVFDILM
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
NVEGVHPFQMRKFEELKSDIAYLNKLSLNSAIDFILSDLEYSQYIREYSSKFKQNQEDLL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
DIVEEFKSSAMEFKSIITFLSHIESVEENLENLNSKGNIDSVILSTMHGVKGMQFNDVHI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCEECCEEE
VNIVDETIPHKNSMENLEEERRLFYVGITRAINNLYLYAPKSVRGKFKEPSIFLNNTNLD
EEEHHHHCCCCCCHHHHHHHHHEEEEEHHHHHCCEEEECCHHHCCCCCCCEEEEECCCCC
SQCNITDYGLKEGDVIRHNYFGIGEILKIEKDTISIKFIQGENKKFSLRTLIENNLIEKI
CCCCCEECCCCCCCEEEECCCCCCHHEEEECCEEEEEEEECCCCCCHHHHHHHHHHHHHH
ICEV
HHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA