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The map label for this gene is cbiP

Identifier: 18310027

GI number: 18310027

Start: 1245586

End: 1247049

Strand: Direct

Name: cbiP

Synonym: CPE1045

Alternate gene names: 18310027

Gene position: 1245586-1247049 (Clockwise)

Preceding gene: 18310026

Following gene: 18310028

Centisome position: 41.09

GC content: 29.71

Gene sequence:

>1464_bases
ATGAAGTATAAAAGTATAATGCTTCTTGGTACAGCATCTTCCGTAGGAAAAAGTACTGTTGCAGCAGCCTTTTGTAGATA
TTTTAAGAAAAAAGGATATAGGGTTGCTCCTTATAAAGCTTTGAATATATCATTAAATTCCTTTGTAACAAAGGAGGGGG
ATGAAATAGGACGAGCTCAAGTGGTACAAGCTGAAGCCTGTGAAATTGATCCTAAGGAATATATGAATCCTATATTGATG
AAACCTAGTGCAGGATTTAAAACTCAGGTAATAGTTAGAGGTAAGGTTCATTGCACTATGGATGCTTATAAATATAAGGA
ATTAAATAAATATCTTAAGGAAAAAGCAAAAGAAGCTTATGATGACATTTCAAATGATTATGATTTAATAGTTTTAGAAG
GATCAGGAAGCTGTGCTGAAATAAACTTAAGAGAAACTGATATAGCAAATATGCATACTGCTAAAATAGCAGACGCTGAT
GTTATTTTAGTTGCTGATATAAACAGAGGAGGAGTTTTTGCTTCTATTGTTGGTACTATAATGCTTTTAACTGAAGAAGA
GAGAAAAAGGGTTAAAGGGGTTATAATAAATAAATTTAGGGGAAAAAGAGAGTTTTTTGAACCTGCAATGAGACAAATAG
AAGAAATAATAAAAATCCCTGTTTTAGGAGTTATGCCTTACTTTGATTTAGATATAGAAGAGGAAGATAGTGCTTCTATT
AAATTAAGAAAAGGTAATGGTAAAGGGATAGATATAGCAATAGTTAGATTACCTCATATGTCAAACTTTACTGATTTTAA
TTCCTTAGGAAGAATAAAAGATGTAGGTATAAGGTATGCTGAGAATCCTAAGGATTTAGAAAATGCAAATATGATAATAA
TTCCAGGAAGTAAAAATACCATAGATGATTTAATTTATTTAAAAGAAAGTGGTTTTAAAGAGGCGCTAATTAATGAAAGT
TCTAATGGAAAATTAATATTTGGTATATGTGGAGGTTATCAAATATTAGGAGAAAAAATAATTGATTCTCTTGGTGTTGA
AGGTGACATAAGAGAAGAAGAAGGCTTAGGACTTTTAAATATAGTAACATCATTTAATAAAGAAAAAACTACTAAACAGG
TTGTGGCCTTTGATTTAGAAGGAAATGAAGTAAGTGGATATGAAATTCATAATGGGGAAAGTGTTCCTACAGCTAAAGAG
AATATATGGATAAAAGAGAAAAATGGTAATGTTCTTGGAATGAATAACAAAGAACAAAATGTTTTTGGTACTTATATTCA
TGGAATCTTTGATGAGGGAGACTTTGGAGAAAAGCTTATAAATAAGTTAAAGAAAGAATTAAACATAGAAGAAAGTAATG
AAGTTAATTATAAAGATTATAAAATGAGTCAATATGATAAGCTTTGTGAACTTTTAGAAGAGAATATAGACATGGCATAT
GTTGAGAATCTAATTAGATCTTAA

Upstream 100 bases:

>100_bases
TTTTGATGGACCTTTATTTAAAGAAAGAATAAACTTTGAAACTGGAGATAATATATTAACAGAAACTCATGGATTCTTAG
ATATTAAGTAGGTGTGTTTT

Downstream 100 bases:

>100_bases
TTTTAAACAGTGTATTAATTTTTATTATTTAATACACTGTTTTTATTTTAAAGTAGTATTTATTTTATATAGAACATTAG
ATAAAAATGCATGACTAAGT

Product: cobyric acid synthase

Products: ADP; phosphate; adenosylcobyric acid; L-glutamate

Alternate protein names: NA

Number of amino acids: Translated: 487; Mature: 487

Protein sequence:

>487_residues
MKYKSIMLLGTASSVGKSTVAAAFCRYFKKKGYRVAPYKALNISLNSFVTKEGDEIGRAQVVQAEACEIDPKEYMNPILM
KPSAGFKTQVIVRGKVHCTMDAYKYKELNKYLKEKAKEAYDDISNDYDLIVLEGSGSCAEINLRETDIANMHTAKIADAD
VILVADINRGGVFASIVGTIMLLTEEERKRVKGVIINKFRGKREFFEPAMRQIEEIIKIPVLGVMPYFDLDIEEEDSASI
KLRKGNGKGIDIAIVRLPHMSNFTDFNSLGRIKDVGIRYAENPKDLENANMIIIPGSKNTIDDLIYLKESGFKEALINES
SNGKLIFGICGGYQILGEKIIDSLGVEGDIREEEGLGLLNIVTSFNKEKTTKQVVAFDLEGNEVSGYEIHNGESVPTAKE
NIWIKEKNGNVLGMNNKEQNVFGTYIHGIFDEGDFGEKLINKLKKELNIEESNEVNYKDYKMSQYDKLCELLEENIDMAY
VENLIRS

Sequences:

>Translated_487_residues
MKYKSIMLLGTASSVGKSTVAAAFCRYFKKKGYRVAPYKALNISLNSFVTKEGDEIGRAQVVQAEACEIDPKEYMNPILM
KPSAGFKTQVIVRGKVHCTMDAYKYKELNKYLKEKAKEAYDDISNDYDLIVLEGSGSCAEINLRETDIANMHTAKIADAD
VILVADINRGGVFASIVGTIMLLTEEERKRVKGVIINKFRGKREFFEPAMRQIEEIIKIPVLGVMPYFDLDIEEEDSASI
KLRKGNGKGIDIAIVRLPHMSNFTDFNSLGRIKDVGIRYAENPKDLENANMIIIPGSKNTIDDLIYLKESGFKEALINES
SNGKLIFGICGGYQILGEKIIDSLGVEGDIREEEGLGLLNIVTSFNKEKTTKQVVAFDLEGNEVSGYEIHNGESVPTAKE
NIWIKEKNGNVLGMNNKEQNVFGTYIHGIFDEGDFGEKLINKLKKELNIEESNEVNYKDYKMSQYDKLCELLEENIDMAY
VENLIRS
>Mature_487_residues
MKYKSIMLLGTASSVGKSTVAAAFCRYFKKKGYRVAPYKALNISLNSFVTKEGDEIGRAQVVQAEACEIDPKEYMNPILM
KPSAGFKTQVIVRGKVHCTMDAYKYKELNKYLKEKAKEAYDDISNDYDLIVLEGSGSCAEINLRETDIANMHTAKIADAD
VILVADINRGGVFASIVGTIMLLTEEERKRVKGVIINKFRGKREFFEPAMRQIEEIIKIPVLGVMPYFDLDIEEEDSASI
KLRKGNGKGIDIAIVRLPHMSNFTDFNSLGRIKDVGIRYAENPKDLENANMIIIPGSKNTIDDLIYLKESGFKEALINES
SNGKLIFGICGGYQILGEKIIDSLGVEGDIREEEGLGLLNIVTSFNKEKTTKQVVAFDLEGNEVSGYEIHNGESVPTAKE
NIWIKEKNGNVLGMNNKEQNVFGTYIHGIFDEGDFGEKLINKLKKELNIEESNEVNYKDYKMSQYDKLCELLEENIDMAY
VENLIRS

Specific function: Catalyzes amidations at positions B, D, E, and G on adenosylcobyrinic A,C-diamide. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation

COG id: COG1492

COG function: function code H; Cobyric acid synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GATase cobBQ-type domain

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): COBQ_CLOP1 (Q0TRJ4)

Other databases:

- EMBL:   CP000246
- RefSeq:   YP_695746.1
- ProteinModelPortal:   Q0TRJ4
- STRING:   Q0TRJ4
- GeneID:   4203728
- GenomeReviews:   CP000246_GR
- KEGG:   cpf:CPF_1300
- TIGR:   CPF_1300
- eggNOG:   COG1492
- HOGENOM:   HBG302271
- OMA:   GVDVAPF
- ProtClustDB:   PRK00784
- BioCyc:   CPER195103:CPF_1300-MONOMER
- HAMAP:   MF_00028
- InterPro:   IPR002586
- InterPro:   IPR017929
- InterPro:   IPR004459
- InterPro:   IPR011698
- TIGRFAMs:   TIGR00313

Pfam domain/function: PF01656 CbiA; PF07685 GATase_3

EC number: 6.3.5.10

Molecular weight: Translated: 54632; Mature: 54632

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: PS51274 GATASE_COBBQ

Important sites: ACT_SITE 330-330 ACT_SITE 427-427

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYKSIMLLGTASSVGKSTVAAAFCRYFKKKGYRVAPYKALNISLNSFVTKEGDEIGRAQ
CCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCEECCEEEEEEEHHHHCCCCCCCCCCEE
VVQAEACEIDPKEYMNPILMKPSAGFKTQVIVRGKVHCTMDAYKYKELNKYLKEKAKEAY
EEEHHHCCCCHHHHCCCEEECCCCCCEEEEEEECEEEEEEHHHHHHHHHHHHHHHHHHHH
DDISNDYDLIVLEGSGSCAEINLRETDIANMHTAKIADADVILVADINRGGVFASIVGTI
HHCCCCCEEEEEECCCCEEEEEECCCCCCCCEEEEECCCCEEEEEECCCCCCHHHHHHHH
MLLTEEERKRVKGVIINKFRGKREFFEPAMRQIEEIIKIPVLGVMPYFDLDIEEEDSASI
HEEEHHHHHHHHHHEEEHHCCCHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCEE
KLRKGNGKGIDIAIVRLPHMSNFTDFNSLGRIKDVGIRYAENPKDLENANMIIIPGSKNT
EEECCCCCCEEEEEEEECCCCCCCCHHHCCCCHHCCCEECCCCCCCCCCCEEEECCCCCC
IDDLIYLKESGFKEALINESSNGKLIFGICGGYQILGEKIIDSLGVEGDIREEEGLGLLN
HHHEEEECCCCCHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCCEE
IVTSFNKEKTTKQVVAFDLEGNEVSGYEIHNGESVPTAKENIWIKEKNGNVLGMNNKEQN
HHHHCCCCCCCEEEEEEEECCCEECCEEEECCCCCCCCCCCEEEEECCCCEEECCCCCCC
VFGTYIHGIFDEGDFGEKLINKLKKELNIEESNEVNYKDYKMSQYDKLCELLEENIDMAY
EEEEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHH
VENLIRS
HHHHHCC
>Mature Secondary Structure
MKYKSIMLLGTASSVGKSTVAAAFCRYFKKKGYRVAPYKALNISLNSFVTKEGDEIGRAQ
CCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCEECCEEEEEEEHHHHCCCCCCCCCCEE
VVQAEACEIDPKEYMNPILMKPSAGFKTQVIVRGKVHCTMDAYKYKELNKYLKEKAKEAY
EEEHHHCCCCHHHHCCCEEECCCCCCEEEEEEECEEEEEEHHHHHHHHHHHHHHHHHHHH
DDISNDYDLIVLEGSGSCAEINLRETDIANMHTAKIADADVILVADINRGGVFASIVGTI
HHCCCCCEEEEEECCCCEEEEEECCCCCCCCEEEEECCCCEEEEEECCCCCCHHHHHHHH
MLLTEEERKRVKGVIINKFRGKREFFEPAMRQIEEIIKIPVLGVMPYFDLDIEEEDSASI
HEEEHHHHHHHHHHEEEHHCCCHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCEE
KLRKGNGKGIDIAIVRLPHMSNFTDFNSLGRIKDVGIRYAENPKDLENANMIIIPGSKNT
EEECCCCCCEEEEEEEECCCCCCCCHHHCCCCHHCCCEECCCCCCCCCCCEEEECCCCCC
IDDLIYLKESGFKEALINESSNGKLIFGICGGYQILGEKIIDSLGVEGDIREEEGLGLLN
HHHEEEECCCCCHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCCEE
IVTSFNKEKTTKQVVAFDLEGNEVSGYEIHNGESVPTAKENIWIKEKNGNVLGMNNKEQN
HHHHCCCCCCCEEEEEEEECCCEECCEEEECCCCCCCCCCCEEEEECCCCEEECCCCCCC
VFGTYIHGIFDEGDFGEKLINKLKKELNIEESNEVNYKDYKMSQYDKLCELLEENIDMAY
EEEEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHH
VENLIRS
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; adenosylcobyrinic acid a,c-diamide; L-glutamine; H2O

Specific reaction: 4 ATP + adenosylcobyrinic acid a,c-diamide + 4 L-glutamine + 4 H2O = 4 ADP + 4 phosphate + adenosylcobyric acid + 4 L-glutamate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA