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The map label for this gene is pykA [H]

Identifier: 18310007

GI number: 18310007

Start: 1226692

End: 1227786

Strand: Direct

Name: pykA [H]

Synonym: CPE1025

Alternate gene names: 18310007

Gene position: 1226692-1227786 (Clockwise)

Preceding gene: 18310006

Following gene: 18310009

Centisome position: 40.47

GC content: 26.48

Gene sequence:

>1095_bases
ATGGGGGAAGTTATGGATATTATAGGCAGTATAAACACCAGATTTTTAAGTAAAAGTGAACAGAGCAAAAAGGAAATTTT
TAATAAAATAGATAACATAGTAAAAGGTGGGGGAAATATAATTAGGATGAATCTATCCCATGGCAAGCATAGAGATGTAG
AATGCTGTATAGATTACATAAGAAGCAATCATAAGAATGTAAAAATTCTTTTAGACTTACAGGGAAATAAGATTAGGGTA
GCTAATAATATATATGGAACATTTAAAGTTAATTCTGGTGATTTAGTTTATTTTTGTTCAGAGGAAACTTATGATGCTTA
TCTTAAAAATATTGATAGAAATAAACTTATACCTTTAAATATAAAAAATAAGTTTATATACAATAATACTTTTAAAAAGA
TATACATGAAAGACGCCACTATGGAATTTATAGTAATAAGCAATAACAATGGGTTAATAAAGACTAAGGTTAAATTAGGT
GGTGTAGTTAGAAAAGAAAAGGGATGTAATCTTCCTAATTTAGATAGGAAAAATTGGGGGGTAAGCGAAAAGGATTTAGA
AGATATTAAATTTGCCATAGATAATAAAGTAGATATTATAGATTATTCTTATTGTTCTTATATGGAAGAATGTAGAGAAT
TTAAAAATATTGTTTTTAAAAATTTAAAGTCAAATCAATTTATTCCTAAGCTTTGGGGAAAAATTGAGACTAATGAAGGA
ATAAATAATATTAAAGAAGTTGCTAAGGAATTAGATGGAATAGTTATAGCAAGGGGAGATTTAACAGCAGAAATTGGAAT
ATTAAATGTTCCTATAGTGCAAGAAAAAATATTATATGCTTTAAAAAATGAGAATAAATCAATAATAGTAGCCACAAATG
TACTTTCATCTATTAGAAATAAACAAAATAAGCCTACAATAAATGAATTAAGCGATATATATCATTTTATTAGGTGTGGC
GCAACAGGGTTTATGCTTACTGGAGAAACTAGTACTGGGGAAAATGAAGAGTATGTGGTTACAACCTTAAAAAATAGTAT
AAAATATTACGAAAAGTTATTATCAAAAATAAAAAAGAGATCAAAGAGAGTCTAA

Upstream 100 bases:

>100_bases
TTAGTAATAAATATTATTCTGTTAGGTGTTTTAAATTTATACAATATATAAATAATATTTAAAATTCATTATGCATATAA
ATCAATGAGTAGCAATATTT

Downstream 100 bases:

>100_bases
AAAGACTTTCTTTGATCTTTTATTTTAGATTTTTGTATAAGTTCATAGGATTTTATAACTATTAAGAAGCTTAAATATCC
TAGTAAAGCACCTGTTAATA

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 364; Mature: 363

Protein sequence:

>364_residues
MGEVMDIIGSINTRFLSKSEQSKKEIFNKIDNIVKGGGNIIRMNLSHGKHRDVECCIDYIRSNHKNVKILLDLQGNKIRV
ANNIYGTFKVNSGDLVYFCSEETYDAYLKNIDRNKLIPLNIKNKFIYNNTFKKIYMKDATMEFIVISNNNGLIKTKVKLG
GVVRKEKGCNLPNLDRKNWGVSEKDLEDIKFAIDNKVDIIDYSYCSYMEECREFKNIVFKNLKSNQFIPKLWGKIETNEG
INNIKEVAKELDGIVIARGDLTAEIGILNVPIVQEKILYALKNENKSIIVATNVLSSIRNKQNKPTINELSDIYHFIRCG
ATGFMLTGETSTGENEEYVVTTLKNSIKYYEKLLSKIKKRSKRV

Sequences:

>Translated_364_residues
MGEVMDIIGSINTRFLSKSEQSKKEIFNKIDNIVKGGGNIIRMNLSHGKHRDVECCIDYIRSNHKNVKILLDLQGNKIRV
ANNIYGTFKVNSGDLVYFCSEETYDAYLKNIDRNKLIPLNIKNKFIYNNTFKKIYMKDATMEFIVISNNNGLIKTKVKLG
GVVRKEKGCNLPNLDRKNWGVSEKDLEDIKFAIDNKVDIIDYSYCSYMEECREFKNIVFKNLKSNQFIPKLWGKIETNEG
INNIKEVAKELDGIVIARGDLTAEIGILNVPIVQEKILYALKNENKSIIVATNVLSSIRNKQNKPTINELSDIYHFIRCG
ATGFMLTGETSTGENEEYVVTTLKNSIKYYEKLLSKIKKRSKRV
>Mature_363_residues
GEVMDIIGSINTRFLSKSEQSKKEIFNKIDNIVKGGGNIIRMNLSHGKHRDVECCIDYIRSNHKNVKILLDLQGNKIRVA
NNIYGTFKVNSGDLVYFCSEETYDAYLKNIDRNKLIPLNIKNKFIYNNTFKKIYMKDATMEFIVISNNNGLIKTKVKLGG
VVRKEKGCNLPNLDRKNWGVSEKDLEDIKFAIDNKVDIIDYSYCSYMEECREFKNIVFKNLKSNQFIPKLWGKIETNEGI
NNIKEVAKELDGIVIARGDLTAEIGILNVPIVQEKILYALKNENKSIIVATNVLSSIRNKQNKPTINELSDIYHFIRCGA
TGFMLTGETSTGENEEYVVTTLKNSIKYYEKLLSKIKKRSKRV

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the PEP- utilizing enzyme family [H]

Homologues:

Organism=Homo sapiens, GI33286422, Length=353, Percent_Identity=27.7620396600567, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI33286420, Length=353, Percent_Identity=27.7620396600567, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI33286418, Length=351, Percent_Identity=27.6353276353276, Blast_Score=123, Evalue=2e-28,
Organism=Homo sapiens, GI32967597, Length=354, Percent_Identity=25.7062146892655, Blast_Score=115, Evalue=9e-26,
Organism=Homo sapiens, GI10835121, Length=354, Percent_Identity=25.7062146892655, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI310128732, Length=276, Percent_Identity=28.2608695652174, Blast_Score=95, Evalue=9e-20,
Organism=Homo sapiens, GI310128730, Length=276, Percent_Identity=28.2608695652174, Blast_Score=95, Evalue=9e-20,
Organism=Homo sapiens, GI310128736, Length=224, Percent_Identity=27.2321428571429, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI310128734, Length=224, Percent_Identity=27.2321428571429, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI310128738, Length=224, Percent_Identity=27.2321428571429, Blast_Score=73, Evalue=3e-13,
Organism=Escherichia coli, GI1787965, Length=320, Percent_Identity=27.1875, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1788160, Length=338, Percent_Identity=26.6272189349112, Blast_Score=96, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17544584, Length=346, Percent_Identity=27.7456647398844, Blast_Score=116, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI71984413, Length=330, Percent_Identity=29.6969696969697, Blast_Score=115, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI17506831, Length=330, Percent_Identity=29.6969696969697, Blast_Score=115, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI71984406, Length=330, Percent_Identity=29.6969696969697, Blast_Score=115, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI17506829, Length=330, Percent_Identity=29.6969696969697, Blast_Score=115, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6319279, Length=328, Percent_Identity=29.8780487804878, Blast_Score=125, Evalue=8e-30,
Organism=Saccharomyces cerevisiae, GI6324923, Length=318, Percent_Identity=30.5031446540881, Blast_Score=119, Evalue=7e-28,
Organism=Drosophila melanogaster, GI24648964, Length=340, Percent_Identity=27.3529411764706, Blast_Score=119, Evalue=2e-27,
Organism=Drosophila melanogaster, GI28571814, Length=340, Percent_Identity=27.3529411764706, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI24648966, Length=346, Percent_Identity=28.3236994219653, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI24581235, Length=338, Percent_Identity=25.7396449704142, Blast_Score=88, Evalue=9e-18,
Organism=Drosophila melanogaster, GI24646914, Length=187, Percent_Identity=29.4117647058824, Blast_Score=84, Evalue=2e-16,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 41684; Mature: 41553

Theoretical pI: Translated: 9.69; Mature: 9.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGEVMDIIGSINTRFLSKSEQSKKEIFNKIDNIVKGGGNIIRMNLSHGKHRDVECCIDYI
CCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHH
RSNHKNVKILLDLQGNKIRVANNIYGTFKVNSGDLVYFCSEETYDAYLKNIDRNKLIPLN
HCCCCCEEEEEEECCCEEEEEECEEEEEEECCCCEEEEECCCHHHHHHHCCCCCCEEEEE
IKNKFIYNNTFKKIYMKDATMEFIVISNNNGLIKTKVKLGGVVRKEKGCNLPNLDRKNWG
CCCEEEECCCEEEEEEECCEEEEEEEECCCCEEEEEEECCCEEECCCCCCCCCCCCCCCC
VSEKDLEDIKFAIDNKVDIIDYSYCSYMEECREFKNIVFKNLKSNQFIPKLWGKIETNEG
CCCCCHHHHHHHHCCCEEEEECHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCEECCCC
INNIKEVAKELDGIVIARGDLTAEIGILNVPIVQEKILYALKNENKSIIVATNVLSSIRN
HHHHHHHHHHHCCEEEECCCCEEEEEEEECCHHHHHHHHHHCCCCCEEEEEHHHHHHHHC
KQNKPTINELSDIYHFIRCGATGFMLTGETSTGENEEYVVTTLKNSIKYYEKLLSKIKKR
CCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHH
SKRV
HCCC
>Mature Secondary Structure 
GEVMDIIGSINTRFLSKSEQSKKEIFNKIDNIVKGGGNIIRMNLSHGKHRDVECCIDYI
CHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHH
RSNHKNVKILLDLQGNKIRVANNIYGTFKVNSGDLVYFCSEETYDAYLKNIDRNKLIPLN
HCCCCCEEEEEEECCCEEEEEECEEEEEEECCCCEEEEECCCHHHHHHHCCCCCCEEEEE
IKNKFIYNNTFKKIYMKDATMEFIVISNNNGLIKTKVKLGGVVRKEKGCNLPNLDRKNWG
CCCEEEECCCEEEEEEECCEEEEEEEECCCCEEEEEEECCCEEECCCCCCCCCCCCCCCC
VSEKDLEDIKFAIDNKVDIIDYSYCSYMEECREFKNIVFKNLKSNQFIPKLWGKIETNEG
CCCCCHHHHHHHHCCCEEEEECHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCEECCCC
INNIKEVAKELDGIVIARGDLTAEIGILNVPIVQEKILYALKNENKSIIVATNVLSSIRN
HHHHHHHHHHHCCEEEECCCCEEEEEEEECCHHHHHHHHHHCCCCCEEEEEHHHHHHHHC
KQNKPTINELSDIYHFIRCGATGFMLTGETSTGENEEYVVTTLKNSIKYYEKLLSKIKKR
CCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHH
SKRV
HCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7549104 [H]