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The map label for this gene is rfbX [H]

Identifier: 18309605

GI number: 18309605

Start: 778916

End: 780367

Strand: Direct

Name: rfbX [H]

Synonym: CPE0623

Alternate gene names: 18309605

Gene position: 778916-780367 (Clockwise)

Preceding gene: 18309604

Following gene: 18309606

Centisome position: 25.69

GC content: 23.48

Gene sequence:

>1452_bases
ATGAGTAAATCAATATCGAAAAATATAATATTTAAATTCTTATTAAATATTTTTAATGTAGTAGTTCCAATAATTATAGG
TCCTTATGTATTAAGAGTTTTAGGACCAGACTTAATGGGAACAATAAACTTCTCCCAAACAATATATGGATACTTTTTCA
TATTTGCAGGTTTTGGAGTATATCAATATGGTTTAAGAGAAATAAGTAGAGTTAGAGACGATAAAAAAAAGCTCTCACAG
GTATATACCAGTCTATTTACTCTAACCTTTATAACAAATATACTTACAACAATTGGGTATGTATTATTTGTTCAGTTTAA
CTATTCTGGAACAGAAATATATGTTGCTTGTATGATATTAACTTTTAATTTATTAGCTAATATCTTTTATACTGAATGGG
TAAATGAAGGATTAGAAAACTATGACTTCATAACAATAAAAACTATTATAATTAGAATTGTTTATGTAGTATTTTTATTT
ATACTTGTTAGAAGTGCAAATGACTTAAAAGAATATATGATTCTTTTAGTTTTATCAACATTTTTAAATAATATTGTAAG
TTTTATATACATAAGAAAAAGAATACCTTTTGATTTTTCTGACTTAAAATTATTAAGGCATATAAAACCTATGTTTTTAG
TTGTTATATTATCAAATGCCAATGTATTATACACTCAACTTGATAGATATTTTATAGGAGAGTATATAAATATGGATTCT
GTTGCTTATTATACAACAGCGCAAAATATAAGTAACATAATAAATACCTTGTTGCTTACAGTTATTTATGCAACTATTCC
AAGAATGTCAAATTATATAGCAAATGAAAATCATGATGAATATAAAGGTTTATTAGATAAAATTTCTAAAATGTATTTTA
TAGTTCTTTTCCCAGCTGCTATAGGTATGTTGGTTTTAGCTAAAGAGGTAATTCTTATATATGGTGGTAGTAAGTATTTA
GATGCTATACCAATGCTTAAGGTTTTTGCTATATATATAATTACTTTAGGATTTGAGACCATATTAAGTAATCAGGTTAT
GTATATAAGAGGAAAAGAAAAAGAACAAGTAAAGATAACATTTATAGGGGGAGCAGTAAACCTAATATTAAATGTAACAT
TATTATGGACAGGTTATTTCAATGGAACAAATGCAGTAATAACAACAATGCTAGCTAATATAGTTGTTATTATATTAGAA
TACTTATATATAAAATTTGTTATGGACTTAGATTTTAACATTTTTAGCCTAGATAAGATGAAGTATCTAGCAATATCATT
ATTATTTATACCTATAACTTATTTTATAGGAAAATTTATAAGTGGAGTTATACTATTTACTTTAGCAGTAATGATTATAA
ACTCATTAGTTTATTTCTTAATTTTACTAGTTATTAAAGATGATTGTTTATTAGAAATAATAAATAAATTTACTAAAAAA
TTAAAAAGATAA

Upstream 100 bases:

>100_bases
CACCTATAAAGGAATGGGATTATGTAGAAGCACCAACAAATCAAGAAAAGTTAAAGGGTGGAGACATAGTAATGATAGAA
TATTATAATTAGGAGGAACT

Downstream 100 bases:

>100_bases
AAAATAAAAGGGGGAGTTTCATTGATTAAAAAGTTTATTCTTGCTACAGTTATTACTTTAAGTGTAACTTCAATTAATGT
TCTAGCTTCAGAGAATGTAA

Product: capsular polysaccharide synthesis protein

Products: lipopolysaccharide [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 483; Mature: 482

Protein sequence:

>483_residues
MSKSISKNIIFKFLLNIFNVVVPIIIGPYVLRVLGPDLMGTINFSQTIYGYFFIFAGFGVYQYGLREISRVRDDKKKLSQ
VYTSLFTLTFITNILTTIGYVLFVQFNYSGTEIYVACMILTFNLLANIFYTEWVNEGLENYDFITIKTIIIRIVYVVFLF
ILVRSANDLKEYMILLVLSTFLNNIVSFIYIRKRIPFDFSDLKLLRHIKPMFLVVILSNANVLYTQLDRYFIGEYINMDS
VAYYTTAQNISNIINTLLLTVIYATIPRMSNYIANENHDEYKGLLDKISKMYFIVLFPAAIGMLVLAKEVILIYGGSKYL
DAIPMLKVFAIYIITLGFETILSNQVMYIRGKEKEQVKITFIGGAVNLILNVTLLWTGYFNGTNAVITTMLANIVVIILE
YLYIKFVMDLDFNIFSLDKMKYLAISLLFIPITYFIGKFISGVILFTLAVMIINSLVYFLILLVIKDDCLLEIINKFTKK
LKR

Sequences:

>Translated_483_residues
MSKSISKNIIFKFLLNIFNVVVPIIIGPYVLRVLGPDLMGTINFSQTIYGYFFIFAGFGVYQYGLREISRVRDDKKKLSQ
VYTSLFTLTFITNILTTIGYVLFVQFNYSGTEIYVACMILTFNLLANIFYTEWVNEGLENYDFITIKTIIIRIVYVVFLF
ILVRSANDLKEYMILLVLSTFLNNIVSFIYIRKRIPFDFSDLKLLRHIKPMFLVVILSNANVLYTQLDRYFIGEYINMDS
VAYYTTAQNISNIINTLLLTVIYATIPRMSNYIANENHDEYKGLLDKISKMYFIVLFPAAIGMLVLAKEVILIYGGSKYL
DAIPMLKVFAIYIITLGFETILSNQVMYIRGKEKEQVKITFIGGAVNLILNVTLLWTGYFNGTNAVITTMLANIVVIILE
YLYIKFVMDLDFNIFSLDKMKYLAISLLFIPITYFIGKFISGVILFTLAVMIINSLVYFLILLVIKDDCLLEIINKFTKK
LKR
>Mature_482_residues
SKSISKNIIFKFLLNIFNVVVPIIIGPYVLRVLGPDLMGTINFSQTIYGYFFIFAGFGVYQYGLREISRVRDDKKKLSQV
YTSLFTLTFITNILTTIGYVLFVQFNYSGTEIYVACMILTFNLLANIFYTEWVNEGLENYDFITIKTIIIRIVYVVFLFI
LVRSANDLKEYMILLVLSTFLNNIVSFIYIRKRIPFDFSDLKLLRHIKPMFLVVILSNANVLYTQLDRYFIGEYINMDSV
AYYTTAQNISNIINTLLLTVIYATIPRMSNYIANENHDEYKGLLDKISKMYFIVLFPAAIGMLVLAKEVILIYGGSKYLD
AIPMLKVFAIYIITLGFETILSNQVMYIRGKEKEQVKITFIGGAVNLILNVTLLWTGYFNGTNAVITTMLANIVVIILEY
LYIKFVMDLDFNIFSLDKMKYLAISLLFIPITYFIGKFISGVILFTLAVMIINSLVYFLILLVIKDDCLLEIINKFTKKL
KR

Specific function: May be involved in the translocation process of the nascent O-polysaccharide molecules and/or its ligation to lipid A core units [H]

COG id: COG2244

COG function: function code R; Membrane protein involved in the export of O-antigen and teichoic acid

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polysaccharide synthase family [H]

Homologues:

Organism=Escherichia coli, GI1788349, Length=374, Percent_Identity=22.4598930481283, Blast_Score=77, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001991
- InterPro:   IPR002797 [H]

Pfam domain/function: PF01943 Polysacc_synt [H]

EC number: NA

Molecular weight: Translated: 55724; Mature: 55592

Theoretical pI: Translated: 9.45; Mature: 9.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKSISKNIIFKFLLNIFNVVVPIIIGPYVLRVLGPDLMGTINFSQTIYGYFFIFAGFGV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHEECCHHHHHHHHHHHHHHHHH
YQYGLREISRVRDDKKKLSQVYTSLFTLTFITNILTTIGYVLFVQFNYSGTEIYVACMIL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHH
TFNLLANIFYTEWVNEGLENYDFITIKTIIIRIVYVVFLFILVRSANDLKEYMILLVLST
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
FLNNIVSFIYIRKRIPFDFSDLKLLRHIKPMFLVVILSNANVLYTQLDRYFIGEYINMDS
HHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHEEEECCCCCCHHHHHHHHHHHHHCCCCC
VAYYTTAQNISNIINTLLLTVIYATIPRMSNYIANENHDEYKGLLDKISKMYFIVLFPAA
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
IGMLVLAKEVILIYGGSKYLDAIPMLKVFAIYIITLGFETILSNQVMYIRGKEKEQVKIT
HHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEEEE
FIGGAVNLILNVTLLWTGYFNGTNAVITTMLANIVVIILEYLYIKFVMDLDFNIFSLDKM
EECHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHH
KYLAISLLFIPITYFIGKFISGVILFTLAVMIINSLVYFLILLVIKDDCLLEIINKFTKK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
LKR
HCC
>Mature Secondary Structure 
SKSISKNIIFKFLLNIFNVVVPIIIGPYVLRVLGPDLMGTINFSQTIYGYFFIFAGFGV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHEECCHHHHHHHHHHHHHHHHH
YQYGLREISRVRDDKKKLSQVYTSLFTLTFITNILTTIGYVLFVQFNYSGTEIYVACMIL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHH
TFNLLANIFYTEWVNEGLENYDFITIKTIIIRIVYVVFLFILVRSANDLKEYMILLVLST
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
FLNNIVSFIYIRKRIPFDFSDLKLLRHIKPMFLVVILSNANVLYTQLDRYFIGEYINMDS
HHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHEEEECCCCCCHHHHHHHHHHHHHCCCCC
VAYYTTAQNISNIINTLLLTVIYATIPRMSNYIANENHDEYKGLLDKISKMYFIVLFPAA
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
IGMLVLAKEVILIYGGSKYLDAIPMLKVFAIYIITLGFETILSNQVMYIRGKEKEQVKIT
HHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEEEE
FIGGAVNLILNVTLLWTGYFNGTNAVITTMLANIVVIILEYLYIKFVMDLDFNIFSLDKM
EECHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHH
KYLAISLLFIPITYFIGKFISGVILFTLAVMIINSLVYFLILLVIKDDCLLEIINKFTKK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
LKR
HCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: lipopolysaccharide [Cytoplasm] [C]

Specific reaction: lipopolysaccharide [Cytoplasm] = lipopolysaccharide [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7692219 [H]