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The map label for this gene is pit [H]

Identifier: 18309565

GI number: 18309565

Start: 723576

End: 724574

Strand: Direct

Name: pit [H]

Synonym: CPE0583

Alternate gene names: 18309565

Gene position: 723576-724574 (Clockwise)

Preceding gene: 18309564

Following gene: 18309569

Centisome position: 23.87

GC content: 34.33

Gene sequence:

>999_bases
ATGTCTAGTACTTTAGCTGTAACAGTTGTTATTATTCTCTTAACATTAGCTTTCGATTTTATAAATGGATTTCATGATAC
TGCAACGGCAGTTGCTACATCAATTTCCACAAGAGCTCTTTCACCTAAGGCTGCCATAGCTTTATGTGCCGTATTTAACT
TTGTTGGAGCATTTTTAGGAACTGAAGTTGCTAAAACTGTGGGAGATGGTATAGTAGATCAAAATAAAATACCTCTATGG
GTTATTGCAGCAGTTATATTAGGAGCTATTATATGGAATCTTATTACTTGGTATTTTGGAATTCCAAGTAGTTCTTCTCA
TGCCTTAATAGGATCATTAATAGGTGGAGGAATAGCTTATTCACATTCAATAAACATAGTAAGTTGGGGAACTCTTTTTG
ATGAAGTAATTCTTTGGCTTATATTATCACCAATTATAGGTTTAATAGTTGGATACATATTTATGATTTTATTAAACTGG
GCACTAAGAAGATGCAAACCAGGCCCTGTAAATAGAATTTTCTCAAAATTACAAGTGGTTTCAGGAGCATTAATGGCCTT
AAACCATGGTGGTAATGATGCTCAAAAATCAATGGGTATAATTACTATGGCACTTTTAAGTGGAGGATTTTTATCTACTT
TTGAAGTACCAACTTGGGTTATAGCTTTATGTGCAATTGCAATGGCAACAGGAACTTCAATAGGTGGTCAAAAAATAATT
AAAACAATGGGAAGTGGAGTTGCTAAATTAAATGCTGTTAATGGATTTGCAGCACAAACTGGGGCAGCTGGAGTTATAGG
AATAGCTACTATGTTCCATGCACCTGTAAGTACAACTCACATAATAACAACTACCATAATGGGAGTTGGAGCTTCTAAAA
ACCCTAAAGCGGTTAAATGGGGAGTTGCTAAGAATATAGTTTGGGCTTGGATATTAACAATCCCAATAAGTGCATGCATA
GCTGCTATAGTAGTTGAAATATTTAAATTTGTATTATAG

Upstream 100 bases:

>100_bases
CTTGATATAATAAAATGGAGAGAAATATATAAATTCTTAGAGGCAACTTTAGATAGTTGTGAGGCTGTAGCCAATATAGT
TGAGGGAGTAGTGATGAAGT

Downstream 100 bases:

>100_bases
TTTGAAAAAGTAAGTATATAAATATAATAGAAAATTTTAATTAAAGATTAGATAGAATCCTAAAAAGAATTTCTATCTAA
TCTTTTTTATTGCTTAGTAG

Product: phosphate ABC transporter permease

Products: Proton [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 332; Mature: 331

Protein sequence:

>332_residues
MSSTLAVTVVIILLTLAFDFINGFHDTATAVATSISTRALSPKAAIALCAVFNFVGAFLGTEVAKTVGDGIVDQNKIPLW
VIAAVILGAIIWNLITWYFGIPSSSSHALIGSLIGGGIAYSHSINIVSWGTLFDEVILWLILSPIIGLIVGYIFMILLNW
ALRRCKPGPVNRIFSKLQVVSGALMALNHGGNDAQKSMGIITMALLSGGFLSTFEVPTWVIALCAIAMATGTSIGGQKII
KTMGSGVAKLNAVNGFAAQTGAAGVIGIATMFHAPVSTTHIITTTIMGVGASKNPKAVKWGVAKNIVWAWILTIPISACI
AAIVVEIFKFVL

Sequences:

>Translated_332_residues
MSSTLAVTVVIILLTLAFDFINGFHDTATAVATSISTRALSPKAAIALCAVFNFVGAFLGTEVAKTVGDGIVDQNKIPLW
VIAAVILGAIIWNLITWYFGIPSSSSHALIGSLIGGGIAYSHSINIVSWGTLFDEVILWLILSPIIGLIVGYIFMILLNW
ALRRCKPGPVNRIFSKLQVVSGALMALNHGGNDAQKSMGIITMALLSGGFLSTFEVPTWVIALCAIAMATGTSIGGQKII
KTMGSGVAKLNAVNGFAAQTGAAGVIGIATMFHAPVSTTHIITTTIMGVGASKNPKAVKWGVAKNIVWAWILTIPISACI
AAIVVEIFKFVL
>Mature_331_residues
SSTLAVTVVIILLTLAFDFINGFHDTATAVATSISTRALSPKAAIALCAVFNFVGAFLGTEVAKTVGDGIVDQNKIPLWV
IAAVILGAIIWNLITWYFGIPSSSSHALIGSLIGGGIAYSHSINIVSWGTLFDEVILWLILSPIIGLIVGYIFMILLNWA
LRRCKPGPVNRIFSKLQVVSGALMALNHGGNDAQKSMGIITMALLSGGFLSTFEVPTWVIALCAIAMATGTSIGGQKIIK
TMGSGVAKLNAVNGFAAQTGAAGVIGIATMFHAPVSTTHIITTTIMGVGASKNPKAVKWGVAKNIVWAWILTIPISACIA
AIVVEIFKFVL

Specific function: Low-affinity inorganic phosphate transport [H]

COG id: COG0306

COG function: function code P; Phosphate/sulphate permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family. Pit subfamily [H]

Homologues:

Organism=Homo sapiens, GI31543630, Length=170, Percent_Identity=30.5882352941176, Blast_Score=93, Evalue=3e-19,
Organism=Homo sapiens, GI5803173, Length=164, Percent_Identity=29.2682926829268, Blast_Score=86, Evalue=6e-17,
Organism=Escherichia coli, GI1789907, Length=226, Percent_Identity=36.283185840708, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1789360, Length=222, Percent_Identity=35.1351351351351, Blast_Score=112, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17539280, Length=177, Percent_Identity=28.2485875706215, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI25146401, Length=170, Percent_Identity=29.4117647058824, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI71981576, Length=169, Percent_Identity=27.2189349112426, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17536725, Length=166, Percent_Identity=28.9156626506024, Blast_Score=79, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI32566716, Length=172, Percent_Identity=25.5813953488372, Blast_Score=76, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6319773, Length=159, Percent_Identity=31.4465408805031, Blast_Score=73, Evalue=8e-14,
Organism=Drosophila melanogaster, GI21356511, Length=181, Percent_Identity=25.414364640884, Blast_Score=75, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001204 [H]

Pfam domain/function: PF01384 PHO4 [H]

EC number: NA

Molecular weight: Translated: 34738; Mature: 34607

Theoretical pI: Translated: 9.98; Mature: 9.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSTLAVTVVIILLTLAFDFINGFHDTATAVATSISTRALSPKAAIALCAVFNFVGAFLG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
TEVAKTVGDGIVDQNKIPLWVIAAVILGAIIWNLITWYFGIPSSSSHALIGSLIGGGIAY
HHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCE
SHSINIVSWGTLFDEVILWLILSPIIGLIVGYIFMILLNWALRRCKPGPVNRIFSKLQVV
ECCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
SGALMALNHGGNDAQKSMGIITMALLSGGFLSTFEVPTWVIALCAIAMATGTSIGGQKII
HHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
KTMGSGVAKLNAVNGFAAQTGAAGVIGIATMFHAPVSTTHIITTTIMGVGASKNPKAVKW
HHHCCCHHHHHHCCCHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCEEH
GVAKNIVWAWILTIPISACIAAIVVEIFKFVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure 
SSTLAVTVVIILLTLAFDFINGFHDTATAVATSISTRALSPKAAIALCAVFNFVGAFLG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
TEVAKTVGDGIVDQNKIPLWVIAAVILGAIIWNLITWYFGIPSSSSHALIGSLIGGGIAY
HHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCE
SHSINIVSWGTLFDEVILWLILSPIIGLIVGYIFMILLNWALRRCKPGPVNRIFSKLQVV
ECCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
SGALMALNHGGNDAQKSMGIITMALLSGGFLSTFEVPTWVIALCAIAMATGTSIGGQKII
HHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
KTMGSGVAKLNAVNGFAAQTGAAGVIGIATMFHAPVSTTHIITTTIMGVGASKNPKAVKW
HHHCCCHHHHHHCCCHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCEEH
GVAKNIVWAWILTIPISACIAAIVVEIFKFVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; phosphate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + phosphate [Periplasm] = Proton [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]