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The map label for this gene is spoT [H]

Identifier: 182682317

GI number: 182682317

Start: 2005951

End: 2008122

Strand: Reverse

Name: spoT [H]

Synonym: XfasM23_1800

Alternate gene names: 182682317

Gene position: 2008122-2005951 (Counterclockwise)

Preceding gene: 182682320

Following gene: 182682316

Centisome position: 79.19

GC content: 51.43

Gene sequence:

>2172_bases
ATGACCCATGTTTCCTTGATCCAGACAGATCACGCCGCACTCCCGTTGGCTGATACGAAGTTACCTGATTACTTCTTGCA
GCTGGAGCGCATGGCTGCTCATTTAACACATGAGCAATTGATGGCGCTGCGTCGCGCCTGGGTGATGGGTATGGCTGCGC
ATGCTGGGCAGACACGTAAATCTGGTGAGCCGTATATCACCCATCCTGTGGCTGTGGCTTGTGTATTGGCCGACTTAGGT
ATGGATGTAGAGACATTAATTGCGGCGATCCTTCACGACACCATTGAGGACACCTCAGTGACCCGTGAAATACTCGCTGC
CGAGTTTGGCGAGACCGTTGCGGATTTGGTGGATGGTGTCACTAAGTTAGACAAACTGAAGTTCCGGAATCGTCAGGAAG
CAGCCGCTGAGAGCTTCCGTAAAATGCTGTTAGCGATGTCGCGTGATTTGCGTGTGATCATGATCAAATTGGCTGATCGC
TTGCACAATATGCGCACCCTTGGCGCGCAGAGTATTGAGGCGCGTAGCCGCATTGCACGTGAGACGCTGGAGATTTACGC
GCCGATTGCTCAGCGTCTTGGCATGGCACTGATGAAATCCGAGCTTCAAAACCTTGGATTTCGTGCATTGCATCCGTGGC
GCCACGCCATCATTGAGAAACATATTCTCAACCAACCCGTGGTGCGCCGTGAGGCGATGGAACGCGTTGAATTGCAGTTG
TCCGGGCGCTTGATACAAGAGGGAGTGCAGCACCGTGTAGTGAGTCGGATCAAGACTCCGTGGAGCATCTACAACAAGAT
GCACGACGATAACAAATCTTTCGACCAAGTGATGGATGTCTTCGGATTTCGATTGGTTGTTAAGAGCGTTGCTGATTGTT
ACCACGCATTGGGTATCGTGCATGGCACTTTAAAACCTTTAGATGGACGGTTCCGGGATTTCATCGCGATTCCCAAAACA
AATGGCTACCAGTCGCTGCATACTGTTCTATTTGGCCCATACGGCTCCCCGATCGAAGTGCAGATTCGTACCGAAGAGAT
GGATATGATTGCTGAACGCGGCATTGCCGCACACTGGGCCTACAAGTGTGGCAGCGATTCGCCGAATAGCGCGCAGAGCC
GCGTTCATGCCTGGATTGCCGAATTGCTCGAATCACAGCGCACCGCGATTTCCTCAATGGAATTCTTGGATAACTTCAAG
GTTGATCTATTTCCAGATGAGATCTATTTATTCACGCCCAAGGGCAAGATCTTTGCTTTGCCAAAAAATTCCACTGCTCT
TGATTTCGCTTATGCGGTACATACCGATATTGGCAATCATGCCGTGACCTCTCGGGTGGATAAACAGCTGATGCCACTGC
GTACCAAACTCTCTAGTGGACAAACTGTGGAAATCATCACGGCACGCTCTGCCTTACCCAAACCGCAATGGTTGGAATTC
GTAGTCAGCAGCAAGGCACGTACTGCAATCCGCCATCAACTCAAACAGCTTCAACACGAGGATGCCGTACAACTGGGTCA
CCGAATGCTTGATCGTGCGTTGGAAGCCATGGACAGCTCGTTGGAACGTCTGCCAAAGGGCCGACTGGATGCCTTCCTGG
CTGAACATCACTTTCAGCGCTTAGAGGCCTTGCTTGCTGACATCGCCTTGGGTAACTGGATGCCTAATCAGACTGCACAA
GCATTGCTGACTTACACGGAATTGCTTGGCAACACGCCGATTGAGCACTCACGCGAGAAAATTTTGATCAATGGTAGCGA
GCGTGGCGTCATCAGCTTTGCAAACTGTTGTCAACCAATTCCTGGTGATGATGTGATGGGCTACCACACCACTGGAAAGG
GGATTGTTGTCCATCGCCTGGATTGTCCAAACCTGAATGAATTGCGTAAATCACCTGAGCGTTGGGTGCCGATCGATTGG
GATTTAAACGTTTCTGGTGATTACGATGCCTCGTTACTCGTCGAAGCTGAGAATCGCACTGGCGTACTGGCCCAATTAGC
TGCGGCGATCGCGCAGAGCCATTCCAATATTGAACATGTTGACTACCTGGAGCGCGACTTTAATGCAGCCCTACTGCGCT
TCAGTATCCAGGTGCGTGACCGCCGTCACCTAGCCGAAGTGATGCGCCGGTTACGCCGCTTGCCTGCAGTACAGAGCGTA
TATCGTCAATAG

Upstream 100 bases:

>100_bases
CGCATCTCTATGATTGTGAACCTGGATAGGCTTTATCGTCGCGGCCTGTCGCATTAGATGCTATTTGCTGTTGCTCATAG
GGTGGCATAGTCTTCCGAAT

Downstream 100 bases:

>100_bases
CTTCCGTCCTTGATACCAGCGTTTTGACTAAGGTATTAGTAGATGAGACCTGCACCTCATGCACTCTTTCTCCACTGGCT
TTCTTCTGCAACTGGAGCAT

Product: (p)ppGpp synthetase I SpoT/RelA

Products: NA

Alternate protein names: Penta-phosphate guanosine-3'-pyrophosphohydrolase; (ppGpp)ase [H]

Number of amino acids: Translated: 723; Mature: 722

Protein sequence:

>723_residues
MTHVSLIQTDHAALPLADTKLPDYFLQLERMAAHLTHEQLMALRRAWVMGMAAHAGQTRKSGEPYITHPVAVACVLADLG
MDVETLIAAILHDTIEDTSVTREILAAEFGETVADLVDGVTKLDKLKFRNRQEAAAESFRKMLLAMSRDLRVIMIKLADR
LHNMRTLGAQSIEARSRIARETLEIYAPIAQRLGMALMKSELQNLGFRALHPWRHAIIEKHILNQPVVRREAMERVELQL
SGRLIQEGVQHRVVSRIKTPWSIYNKMHDDNKSFDQVMDVFGFRLVVKSVADCYHALGIVHGTLKPLDGRFRDFIAIPKT
NGYQSLHTVLFGPYGSPIEVQIRTEEMDMIAERGIAAHWAYKCGSDSPNSAQSRVHAWIAELLESQRTAISSMEFLDNFK
VDLFPDEIYLFTPKGKIFALPKNSTALDFAYAVHTDIGNHAVTSRVDKQLMPLRTKLSSGQTVEIITARSALPKPQWLEF
VVSSKARTAIRHQLKQLQHEDAVQLGHRMLDRALEAMDSSLERLPKGRLDAFLAEHHFQRLEALLADIALGNWMPNQTAQ
ALLTYTELLGNTPIEHSREKILINGSERGVISFANCCQPIPGDDVMGYHTTGKGIVVHRLDCPNLNELRKSPERWVPIDW
DLNVSGDYDASLLVEAENRTGVLAQLAAAIAQSHSNIEHVDYLERDFNAALLRFSIQVRDRRHLAEVMRRLRRLPAVQSV
YRQ

Sequences:

>Translated_723_residues
MTHVSLIQTDHAALPLADTKLPDYFLQLERMAAHLTHEQLMALRRAWVMGMAAHAGQTRKSGEPYITHPVAVACVLADLG
MDVETLIAAILHDTIEDTSVTREILAAEFGETVADLVDGVTKLDKLKFRNRQEAAAESFRKMLLAMSRDLRVIMIKLADR
LHNMRTLGAQSIEARSRIARETLEIYAPIAQRLGMALMKSELQNLGFRALHPWRHAIIEKHILNQPVVRREAMERVELQL
SGRLIQEGVQHRVVSRIKTPWSIYNKMHDDNKSFDQVMDVFGFRLVVKSVADCYHALGIVHGTLKPLDGRFRDFIAIPKT
NGYQSLHTVLFGPYGSPIEVQIRTEEMDMIAERGIAAHWAYKCGSDSPNSAQSRVHAWIAELLESQRTAISSMEFLDNFK
VDLFPDEIYLFTPKGKIFALPKNSTALDFAYAVHTDIGNHAVTSRVDKQLMPLRTKLSSGQTVEIITARSALPKPQWLEF
VVSSKARTAIRHQLKQLQHEDAVQLGHRMLDRALEAMDSSLERLPKGRLDAFLAEHHFQRLEALLADIALGNWMPNQTAQ
ALLTYTELLGNTPIEHSREKILINGSERGVISFANCCQPIPGDDVMGYHTTGKGIVVHRLDCPNLNELRKSPERWVPIDW
DLNVSGDYDASLLVEAENRTGVLAQLAAAIAQSHSNIEHVDYLERDFNAALLRFSIQVRDRRHLAEVMRRLRRLPAVQSV
YRQ
>Mature_722_residues
THVSLIQTDHAALPLADTKLPDYFLQLERMAAHLTHEQLMALRRAWVMGMAAHAGQTRKSGEPYITHPVAVACVLADLGM
DVETLIAAILHDTIEDTSVTREILAAEFGETVADLVDGVTKLDKLKFRNRQEAAAESFRKMLLAMSRDLRVIMIKLADRL
HNMRTLGAQSIEARSRIARETLEIYAPIAQRLGMALMKSELQNLGFRALHPWRHAIIEKHILNQPVVRREAMERVELQLS
GRLIQEGVQHRVVSRIKTPWSIYNKMHDDNKSFDQVMDVFGFRLVVKSVADCYHALGIVHGTLKPLDGRFRDFIAIPKTN
GYQSLHTVLFGPYGSPIEVQIRTEEMDMIAERGIAAHWAYKCGSDSPNSAQSRVHAWIAELLESQRTAISSMEFLDNFKV
DLFPDEIYLFTPKGKIFALPKNSTALDFAYAVHTDIGNHAVTSRVDKQLMPLRTKLSSGQTVEIITARSALPKPQWLEFV
VSSKARTAIRHQLKQLQHEDAVQLGHRMLDRALEAMDSSLERLPKGRLDAFLAEHHFQRLEALLADIALGNWMPNQTAQA
LLTYTELLGNTPIEHSREKILINGSERGVISFANCCQPIPGDDVMGYHTTGKGIVVHRLDCPNLNELRKSPERWVPIDWD
LNVSGDYDASLLVEAENRTGVLAQLAAAIAQSHSNIEHVDYLERDFNAALLRFSIQVRDRRHLAEVMRRLRRLPAVQSVY
RQ

Specific function: In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance [H]

COG id: COG0317

COG function: function code TK; Guanosine polyphosphate pyrophosphohydrolases/synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HD domain [H]

Homologues:

Organism=Escherichia coli, GI1790082, Length=692, Percent_Identity=47.6878612716763, Blast_Score=656, Evalue=0.0,
Organism=Escherichia coli, GI1789147, Length=675, Percent_Identity=33.4814814814815, Blast_Score=374, Evalue=1e-104,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR012675
- InterPro:   IPR003607
- InterPro:   IPR007685
- InterPro:   IPR004811
- InterPro:   IPR004095
- InterPro:   IPR012676 [H]

Pfam domain/function: PF01842 ACT; PF04607 RelA_SpoT; PF02824 TGS [H]

EC number: =3.1.7.2 [H]

Molecular weight: Translated: 81664; Mature: 81533

Theoretical pI: Translated: 7.67; Mature: 7.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHVSLIQTDHAALPLADTKLPDYFLQLERMAAHLTHEQLMALRRAWVMGMAAHAGQTRK
CCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
SGEPYITHPVAVACVLADLGMDVETLIAAILHDTIEDTSVTREILAAEFGETVADLVDGV
CCCCEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TKLDKLKFRNRQEAAAESFRKMLLAMSRDLRVIMIKLADRLHNMRTLGAQSIEARSRIAR
HHHHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHH
ETLEIYAPIAQRLGMALMKSELQNLGFRALHPWRHAIIEKHILNQPVVRREAMERVELQL
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
SGRLIQEGVQHRVVSRIKTPWSIYNKMHDDNKSFDQVMDVFGFRLVVKSVADCYHALGIV
HHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HGTLKPLDGRFRDFIAIPKTNGYQSLHTVLFGPYGSPIEVQIRTEEMDMIAERGIAAHWA
HCCCCCCCCCHHHEEECCCCCCHHHHHHHHCCCCCCCEEEEEEHHHHHHHHHCCCHHHEE
YKCGSDSPNSAQSRVHAWIAELLESQRTAISSMEFLDNFKVDLFPDEIYLFTPKGKIFAL
EECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCEEEEECCCCCEEEE
PKNSTALDFAYAVHTDIGNHAVTSRVDKQLMPLRTKLSSGQTVEIITARSALPKPQWLEF
CCCCCCEEEHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHH
VVSSKARTAIRHQLKQLQHEDAVQLGHRMLDRALEAMDSSLERLPKGRLDAFLAEHHFQR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
LEALLADIALGNWMPNQTAQALLTYTELLGNTPIEHSREKILINGSERGVISFANCCQPI
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCEEEHHHHCCCC
PGDDVMGYHTTGKGIVVHRLDCPNLNELRKSPERWVPIDWDLNVSGDYDASLLVEAENRT
CCCCCCCEEECCCCEEEEECCCCCHHHHHCCCCCCCCCEECCCCCCCCCCEEEEEECCCC
GVLAQLAAAIAQSHSNIEHVDYLERDFNAALLRFSIQVRDRRHLAEVMRRLRRLPAVQSV
CHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHH
YRQ
HCC
>Mature Secondary Structure 
THVSLIQTDHAALPLADTKLPDYFLQLERMAAHLTHEQLMALRRAWVMGMAAHAGQTRK
CCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
SGEPYITHPVAVACVLADLGMDVETLIAAILHDTIEDTSVTREILAAEFGETVADLVDGV
CCCCEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TKLDKLKFRNRQEAAAESFRKMLLAMSRDLRVIMIKLADRLHNMRTLGAQSIEARSRIAR
HHHHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHH
ETLEIYAPIAQRLGMALMKSELQNLGFRALHPWRHAIIEKHILNQPVVRREAMERVELQL
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
SGRLIQEGVQHRVVSRIKTPWSIYNKMHDDNKSFDQVMDVFGFRLVVKSVADCYHALGIV
HHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HGTLKPLDGRFRDFIAIPKTNGYQSLHTVLFGPYGSPIEVQIRTEEMDMIAERGIAAHWA
HCCCCCCCCCHHHEEECCCCCCHHHHHHHHCCCCCCCEEEEEEHHHHHHHHHCCCHHHEE
YKCGSDSPNSAQSRVHAWIAELLESQRTAISSMEFLDNFKVDLFPDEIYLFTPKGKIFAL
EECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCEEEEECCCCCEEEE
PKNSTALDFAYAVHTDIGNHAVTSRVDKQLMPLRTKLSSGQTVEIITARSALPKPQWLEF
CCCCCCEEEHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHH
VVSSKARTAIRHQLKQLQHEDAVQLGHRMLDRALEAMDSSLERLPKGRLDAFLAEHHFQR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
LEALLADIALGNWMPNQTAQALLTYTELLGNTPIEHSREKILINGSERGVISFANCCQPI
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCEEEHHHHCCCC
PGDDVMGYHTTGKGIVVHRLDCPNLNELRKSPERWVPIDWDLNVSGDYDASLLVEAENRT
CCCCCCCEEECCCCEEEEECCCCCHHHHHCCCCCCCCCEECCCCCCCCCCEEEEEECCCC
GVLAQLAAAIAQSHSNIEHVDYLERDFNAALLRFSIQVRDRRHLAEVMRRLRRLPAVQSV
CHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHH
YRQ
HCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]