Click here to switch to the map view.

The map label for this gene is hisF

Identifier: 182681879

GI number: 182681879

Start: 1485656

End: 1486429

Strand: Reverse

Name: hisF

Synonym: XfasM23_1347

Alternate gene names: 182681879

Gene position: 1486429-1485656 (Counterclockwise)

Preceding gene: 182681880

Following gene: 182681878

Centisome position: 58.62

GC content: 56.72

Gene sequence:

>774_bases
ATGTTGAGCCGTCGCATTATTCCATGCTTGGACGTGCGTGATGGACGTGTGGTGAAAGGCGTGAAGTTTCGTGACCACGT
GGATATGGGCGATATCGTCGAACTGGCCTTGCGTTACCGTGATCATGGCGCTGATGAGTTGGTGTTTTATGACATCGGCG
CCAGTCCACAAGGGCGTTCTGTGGATTACCGTTGGGTTGAACGCGTTGCGCGCCTGATCGACATTCCATTCTGTGTGGCC
GGTGGTATCGGCGAGGTTGAGACTGCCCGTGCTGTCTTGCATGCCGGTGCCGATAAGATTTCGATCAACTCACCGGCACT
GCGCCAACCTGCGCTGATTAGCGCCTTAGCCGAAGCCTTTGGGGTGCAATGCGTCGTCGTCGGTATTGACTCGATCCGTG
AGGCTGATGGTCAGTGGCGCGTCCGCTGCAATACCGGTGATCCGGACAAAACCCAGGCCTTGCCGCTGCGCACCCTGGAT
TGGATTGTTGAGGCGCAGCGCCTAGGGGCAGGGGAGATCGTATTAAATTGCATGGACAGCGACGGGGTGCGCTGTGGTTA
TGACATTGCACAGCTGTCTCAGGCGCGGGCATTGTGTCAGGTGCCCTTGGTGGCTTCTGGCGGCGCCGGGGACATGCAGC
ACTTTGCGGATGTATTTCACAAGGCTGATGTTGATGGTGCATTGGCGGCCAGCGTATTCCATAGCGGCGCGATTTCGATT
CCGGGTTTAAAACAGTTTCTGCGTGAGCAGCAAATTGAGGTCCGCGATGTGTAA

Upstream 100 bases:

>100_bases
ATCTCCCGCCGGGGACGCCATGTGCCAATGGGCGGATGCGGCCCTATCGCCGTTGCACACTACACCGTGACCACTTGCAG
CAAGAGGCGCAGAGGCGGTC

Downstream 100 bases:

>100_bases
TGAGCCTGCGACGAGCGATGTTGCATTGCCTGATCTGGATTGGGCCAAGGGTGATGGCTTGTTGCCCGTGATCGTGCAGG
ACGCCGATACGCTGCGTGTG

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF

Number of amino acids: Translated: 257; Mature: 257

Protein sequence:

>257_residues
MLSRRIIPCLDVRDGRVVKGVKFRDHVDMGDIVELALRYRDHGADELVFYDIGASPQGRSVDYRWVERVARLIDIPFCVA
GGIGEVETARAVLHAGADKISINSPALRQPALISALAEAFGVQCVVVGIDSIREADGQWRVRCNTGDPDKTQALPLRTLD
WIVEAQRLGAGEIVLNCMDSDGVRCGYDIAQLSQARALCQVPLVASGGAGDMQHFADVFHKADVDGALAASVFHSGAISI
PGLKQFLREQQIEVRDV

Sequences:

>Translated_257_residues
MLSRRIIPCLDVRDGRVVKGVKFRDHVDMGDIVELALRYRDHGADELVFYDIGASPQGRSVDYRWVERVARLIDIPFCVA
GGIGEVETARAVLHAGADKISINSPALRQPALISALAEAFGVQCVVVGIDSIREADGQWRVRCNTGDPDKTQALPLRTLD
WIVEAQRLGAGEIVLNCMDSDGVRCGYDIAQLSQARALCQVPLVASGGAGDMQHFADVFHKADVDGALAASVFHSGAISI
PGLKQFLREQQIEVRDV
>Mature_257_residues
MLSRRIIPCLDVRDGRVVKGVKFRDHVDMGDIVELALRYRDHGADELVFYDIGASPQGRSVDYRWVERVARLIDIPFCVA
GGIGEVETARAVLHAGADKISINSPALRQPALISALAEAFGVQCVVVGIDSIREADGQWRVRCNTGDPDKTQALPLRTLD
WIVEAQRLGAGEIVLNCMDSDGVRCGYDIAQLSQARALCQVPLVASGGAGDMQHFADVFHKADVDGALAASVFHSGAISI
PGLKQFLREQQIEVRDV

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI1788336, Length=256, Percent_Identity=61.71875, Blast_Score=315, Evalue=2e-87,
Organism=Saccharomyces cerevisiae, GI6319725, Length=315, Percent_Identity=30.1587301587302, Blast_Score=128, Evalue=1e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS6_XYLF2 (B2I5X6)

Other databases:

- EMBL:   CP001011
- RefSeq:   YP_001830039.1
- ProteinModelPortal:   B2I5X6
- SMR:   B2I5X6
- GeneID:   6202212
- GenomeReviews:   CP001011_GR
- KEGG:   xfn:XfasM23_1347
- HOGENOM:   HBG541613
- OMA:   ASVFHKG
- ProtClustDB:   PRK02083
- GO:   GO:0005737
- HAMAP:   MF_01013
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00735

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: 4.1.3.-

Molecular weight: Translated: 27902; Mature: 27902

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: NA

Important sites: ACT_SITE 11-11 ACT_SITE 130-130

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSRRIIPCLDVRDGRVVKGVKFRDHVDMGDIVELALRYRDHGADELVFYDIGASPQGRS
CCCCCCCCEEECCCCCEEECCEECCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCC
VDYRWVERVARLIDIPFCVAGGIGEVETARAVLHAGADKISINSPALRQPALISALAEAF
CCHHHHHHHHHHHCCCHHCCCCCCCHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHH
GVQCVVVGIDSIREADGQWRVRCNTGDPDKTQALPLRTLDWIVEAQRLGAGEIVLNCMDS
CCEEEEEECHHHHCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEECC
DGVRCGYDIAQLSQARALCQVPLVASGGAGDMQHFADVFHKADVDGALAASVFHSGAISI
CCCCCCHHHHHHHHHHHHHHCCEEECCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCCC
PGLKQFLREQQIEVRDV
HHHHHHHHHHCCHHCCC
>Mature Secondary Structure
MLSRRIIPCLDVRDGRVVKGVKFRDHVDMGDIVELALRYRDHGADELVFYDIGASPQGRS
CCCCCCCCEEECCCCCEEECCEECCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCC
VDYRWVERVARLIDIPFCVAGGIGEVETARAVLHAGADKISINSPALRQPALISALAEAF
CCHHHHHHHHHHHCCCHHCCCCCCCHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHH
GVQCVVVGIDSIREADGQWRVRCNTGDPDKTQALPLRTLDWIVEAQRLGAGEIVLNCMDS
CCEEEEEECHHHHCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEECC
DGVRCGYDIAQLSQARALCQVPLVASGGAGDMQHFADVFHKADVDGALAASVFHSGAISI
CCCCCCHHHHHHHHHHHHHHCCEEECCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCCC
PGLKQFLREQQIEVRDV
HHHHHHHHHHCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA