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The map label for this gene is fumB [H]

Identifier: 182681545

GI number: 182681545

Start: 1155253

End: 1156845

Strand: Reverse

Name: fumB [H]

Synonym: XfasM23_1000

Alternate gene names: 182681545

Gene position: 1156845-1155253 (Counterclockwise)

Preceding gene: 182681546

Following gene: 182681543

Centisome position: 45.62

GC content: 52.67

Gene sequence:

>1593_bases
ATGTTTATCCTATGGCTGATGTATTCAGGGGGATTACAATGGCAACGTTTTTTAGTCAGCCATAAGGGCCAGCCGGTGTC
ATTGATCAAACAAGAAGACTTTATCCAGTCCATCGCTGATGCGCTGCAATACATCAGTTACTACCATCCGGTCGATTACA
TCAAGAATCTGGCTTTGGCGTATGGGTGCGAGAAATCGCCCGCCGCTAAAGATGCAATGGCGCAGATTTTGATTAACTCA
CGTATGTGTGCTGAGGGGCGCCGCCCGATTTGCCAAGATACAGGGATTGTCACGGTGTTCCTTAGCATCGGGATGGATGT
ACGTTGGGATGACGTCACGATGGATGTGGAAGAGATGGTGAATGAGGGGGTTCGCCGTGCCTATCGTCATCCGGATAACA
CGCTACGTGCGTCTGTGCTTGCTGATCCTGCCGGCAAGCGCTGCAATACGAGGGACAATACGCCAGCAGTCGTTAACGTC
AAGGTTGTTTCTGGGGGCCATGTTGAAGTGATTGTTGCTGCCAAGGGAGGGGGATCTGAGGCCAAGAGCAAATTTGCAAT
GCTAAATCCATCGGATTCCATCGTTGATTGGGTTCTGGACACTGTGCCAACCATGGGGGCTGGCTGGTGCCCACCAGGGA
TGCTTGGGATTGGTATCGGTGGTACGGCTGAGAAAGCGATGCTGCTCGCTAAGGAAGCGTTGATGGAACCGATTGACATC
CAGGATTTGATGGTACGCGGCCCTTCCAATCGTGCTGAAGAATTACGCTTGGAGTTGTACGAGAAGGTCAATGCTTTAGG
GATCGGTGCTCAGGGGGTGGGCGGTTTGACGACGGTATTAGATATCAAGGTCAAAGATTATCCAACGCATGCTGCCAATT
TGCCGGTAGCGATGATCCCTAATTGTGCGTCCACACGCCATGCGCATTTCACATTGGACGGTAGCGGTCCTGTTGTTCTG
GAACCGCCGTCATTAGAGGATTGGCCTAAGTTGAGCTATCTCCCGGAGTCTGCACGGCAGGTGGATTTGAATACGATCAC
CAGGGATGAGGTGGCGCAGTTCAAGCCAGGTGAGGTGTTGCTACTCAGCGGCAAATTGTTGACGGGACGAGATGCTGCGC
ATAAGCGCATCGTTGAGATGCTCAACAAGGGGGAGACATTACCAGTTGATTTCACGAATCGCTTTATCTACTACGTGGGT
CCAGTGGATCCTGTGCGTGATGAAGTCGTCGGTCCTGCGGGTCCAACCACAGCAACGCGTATGGATAAGTTTACGCGTCA
GATGCTGGAGCAGGGCGGGTTGCTCGGTATGGTGGGCAAGGCCGAGCGTGGTCCTACTGCCATCGCTGCAATCCGTGACA
ACAAAGCCGTGTATCTGATGGCGGTGGGAGGGGCGGCTTATCTTGTCTCCAAGGCGATCAAAACGAGCCGGATACTGGCT
TTTGAAGACCTTGGCATGGAGGCAATTTATGAGTTTGAAGTCAAGGAGATGCCTGTAACAGTGGCGGTTGATTGCAACGG
TGAGTCGATACATACAACTGGGCCGCGTGAGTGGCGAAGCCGGATCGGGAAAATTCCGGTTGTTGTGGAGTAG

Upstream 100 bases:

>100_bases
ACGTTCTAATCTAAAAATAAATTTATTTTTGATGATTGGAAGAAAATTTAAGTGATAAGTAGGGTAGGGATGTAAGCGGT
TTCAGTGGGTGTGGATCGCA

Downstream 100 bases:

>100_bases
GAATGGCGCAACGCTTGGAAGCACTGGAACAGTGGGTTCATTGTCAGGTGGTGCGTTCTCTACGGTGTTGCATCGGGTTG
GTGCGTCATGTGGGCGACAG

Product: tartrate/fumarate subfamily Fe-S type hydro-lyase subunit alpha

Products: NA

Alternate protein names: Fumarase [H]

Number of amino acids: Translated: 530; Mature: 530

Protein sequence:

>530_residues
MFILWLMYSGGLQWQRFLVSHKGQPVSLIKQEDFIQSIADALQYISYYHPVDYIKNLALAYGCEKSPAAKDAMAQILINS
RMCAEGRRPICQDTGIVTVFLSIGMDVRWDDVTMDVEEMVNEGVRRAYRHPDNTLRASVLADPAGKRCNTRDNTPAVVNV
KVVSGGHVEVIVAAKGGGSEAKSKFAMLNPSDSIVDWVLDTVPTMGAGWCPPGMLGIGIGGTAEKAMLLAKEALMEPIDI
QDLMVRGPSNRAEELRLELYEKVNALGIGAQGVGGLTTVLDIKVKDYPTHAANLPVAMIPNCASTRHAHFTLDGSGPVVL
EPPSLEDWPKLSYLPESARQVDLNTITRDEVAQFKPGEVLLLSGKLLTGRDAAHKRIVEMLNKGETLPVDFTNRFIYYVG
PVDPVRDEVVGPAGPTTATRMDKFTRQMLEQGGLLGMVGKAERGPTAIAAIRDNKAVYLMAVGGAAYLVSKAIKTSRILA
FEDLGMEAIYEFEVKEMPVTVAVDCNGESIHTTGPREWRSRIGKIPVVVE

Sequences:

>Translated_530_residues
MFILWLMYSGGLQWQRFLVSHKGQPVSLIKQEDFIQSIADALQYISYYHPVDYIKNLALAYGCEKSPAAKDAMAQILINS
RMCAEGRRPICQDTGIVTVFLSIGMDVRWDDVTMDVEEMVNEGVRRAYRHPDNTLRASVLADPAGKRCNTRDNTPAVVNV
KVVSGGHVEVIVAAKGGGSEAKSKFAMLNPSDSIVDWVLDTVPTMGAGWCPPGMLGIGIGGTAEKAMLLAKEALMEPIDI
QDLMVRGPSNRAEELRLELYEKVNALGIGAQGVGGLTTVLDIKVKDYPTHAANLPVAMIPNCASTRHAHFTLDGSGPVVL
EPPSLEDWPKLSYLPESARQVDLNTITRDEVAQFKPGEVLLLSGKLLTGRDAAHKRIVEMLNKGETLPVDFTNRFIYYVG
PVDPVRDEVVGPAGPTTATRMDKFTRQMLEQGGLLGMVGKAERGPTAIAAIRDNKAVYLMAVGGAAYLVSKAIKTSRILA
FEDLGMEAIYEFEVKEMPVTVAVDCNGESIHTTGPREWRSRIGKIPVVVE
>Mature_530_residues
MFILWLMYSGGLQWQRFLVSHKGQPVSLIKQEDFIQSIADALQYISYYHPVDYIKNLALAYGCEKSPAAKDAMAQILINS
RMCAEGRRPICQDTGIVTVFLSIGMDVRWDDVTMDVEEMVNEGVRRAYRHPDNTLRASVLADPAGKRCNTRDNTPAVVNV
KVVSGGHVEVIVAAKGGGSEAKSKFAMLNPSDSIVDWVLDTVPTMGAGWCPPGMLGIGIGGTAEKAMLLAKEALMEPIDI
QDLMVRGPSNRAEELRLELYEKVNALGIGAQGVGGLTTVLDIKVKDYPTHAANLPVAMIPNCASTRHAHFTLDGSGPVVL
EPPSLEDWPKLSYLPESARQVDLNTITRDEVAQFKPGEVLLLSGKLLTGRDAAHKRIVEMLNKGETLPVDFTNRFIYYVG
PVDPVRDEVVGPAGPTTATRMDKFTRQMLEQGGLLGMVGKAERGPTAIAAIRDNKAVYLMAVGGAAYLVSKAIKTSRILA
FEDLGMEAIYEFEVKEMPVTVAVDCNGESIHTTGPREWRSRIGKIPVVVE

Specific function: It functions in the generation of fumarate for use as an anaerobic electron acceptor [H]

COG id: COG1951

COG function: function code C; Tartrate dehydratase alpha subunit/Fumarate hydratase class I, N-terminal domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I fumarase family [H]

Homologues:

Organism=Escherichia coli, GI1790564, Length=474, Percent_Identity=31.8565400843882, Blast_Score=194, Evalue=2e-50,
Organism=Escherichia coli, GI1787897, Length=469, Percent_Identity=31.9829424307036, Blast_Score=189, Evalue=4e-49,
Organism=Escherichia coli, GI1789442, Length=305, Percent_Identity=26.8852459016393, Blast_Score=89, Evalue=7e-19,
Organism=Escherichia coli, GI1789443, Length=179, Percent_Identity=29.608938547486, Blast_Score=87, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004646
- InterPro:   IPR004647
- InterPro:   IPR011167
- InterPro:   IPR020557 [H]

Pfam domain/function: PF05681 Fumerase; PF05683 Fumerase_C [H]

EC number: =4.2.1.2 [H]

Molecular weight: Translated: 57751; Mature: 57751

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFILWLMYSGGLQWQRFLVSHKGQPVSLIKQEDFIQSIADALQYISYYHPVDYIKNLALA
CEEEEEEECCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
YGCEKSPAAKDAMAQILINSRMCAEGRRPICQDTGIVTVFLSIGMDVRWDDVTMDVEEMV
HCCCCCCCHHHHHHHHHHHCHHHHCCCCCCCCCCCCEEEEECCCCCEEECCCEECHHHHH
NEGVRRAYRHPDNTLRASVLADPAGKRCNTRDNTPAVVNVKVVSGGHVEVIVAAKGGGSE
HHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCEEEEEEEECCCCEEEEEEECCCCCH
AKSKFAMLNPSDSIVDWVLDTVPTMGAGWCPPGMLGIGIGGTAEKAMLLAKEALMEPIDI
HHCEEEEECCCHHHHHHHHHHCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHCCCCCC
QDLMVRGPSNRAEELRLELYEKVNALGIGAQGVGGLTTVLDIKVKDYPTHAANLPVAMIP
HHHEEECCCCHHHHHHHHHHHHHHHEECCCCCCCCEEEEEEEEEECCCCCCCCCCEEECC
NCASTRHAHFTLDGSGPVVLEPPSLEDWPKLSYLPESARQVDLNTITRDEVAQFKPGEVL
CCCCCCCEEEEECCCCCEEECCCCCCCCCCCCCCCCCHHEEECHHCCHHHHHHCCCCCEE
LLSGKLLTGRDAAHKRIVEMLNKGETLPVDFTNRFIYYVGPVDPVRDEVVGPAGPTTATR
EEECCEECCCHHHHHHHHHHHHCCCCCEEEECCCEEEEECCCCCHHHHCCCCCCCCHHHH
MDKFTRQMLEQGGLLGMVGKAERGPTAIAAIRDNKAVYLMAVGGAAYLVSKAIKTSRILA
HHHHHHHHHHCCCEEEECCCCCCCCEEEEEEECCCEEEEEEECCHHHHHHHHHHHHHEEE
FEDLGMEAIYEFEVKEMPVTVAVDCNGESIHTTGPREWRSRIGKIPVVVE
EHHCCCCEEEEEEEECCCEEEEEECCCCEEECCCCHHHHHHHCCCCEEEC
>Mature Secondary Structure
MFILWLMYSGGLQWQRFLVSHKGQPVSLIKQEDFIQSIADALQYISYYHPVDYIKNLALA
CEEEEEEECCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
YGCEKSPAAKDAMAQILINSRMCAEGRRPICQDTGIVTVFLSIGMDVRWDDVTMDVEEMV
HCCCCCCCHHHHHHHHHHHCHHHHCCCCCCCCCCCCEEEEECCCCCEEECCCEECHHHHH
NEGVRRAYRHPDNTLRASVLADPAGKRCNTRDNTPAVVNVKVVSGGHVEVIVAAKGGGSE
HHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCEEEEEEEECCCCEEEEEEECCCCCH
AKSKFAMLNPSDSIVDWVLDTVPTMGAGWCPPGMLGIGIGGTAEKAMLLAKEALMEPIDI
HHCEEEEECCCHHHHHHHHHHCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHCCCCCC
QDLMVRGPSNRAEELRLELYEKVNALGIGAQGVGGLTTVLDIKVKDYPTHAANLPVAMIP
HHHEEECCCCHHHHHHHHHHHHHHHEECCCCCCCCEEEEEEEEEECCCCCCCCCCEEECC
NCASTRHAHFTLDGSGPVVLEPPSLEDWPKLSYLPESARQVDLNTITRDEVAQFKPGEVL
CCCCCCCEEEEECCCCCEEECCCCCCCCCCCCCCCCCHHEEECHHCCHHHHHHCCCCCEE
LLSGKLLTGRDAAHKRIVEMLNKGETLPVDFTNRFIYYVGPVDPVRDEVVGPAGPTTATR
EEECCEECCCHHHHHHHHHHHHCCCCCEEEECCCEEEEECCCCCHHHHCCCCCCCCHHHH
MDKFTRQMLEQGGLLGMVGKAERGPTAIAAIRDNKAVYLMAVGGAAYLVSKAIKTSRILA
HHHHHHHHHHCCCEEEECCCCCCCCEEEEEEECCCEEEEEEECCHHHHHHHHHHHHHEEE
FEDLGMEAIYEFEVKEMPVTVAVDCNGESIHTTGPREWRSRIGKIPVVVE
EHHCCCCEEEEEEEECCCEEEEEECCCCEEECCCCHHHHHHHCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2656658; 7610040; 9278503 [H]