Click here to switch to the map view.

The map label for this gene is thiC [H]

Identifier: 182681504

GI number: 182681504

Start: 1122758

End: 1124614

Strand: Reverse

Name: thiC [H]

Synonym: XfasM23_0958

Alternate gene names: 182681504

Gene position: 1124614-1122758 (Counterclockwise)

Preceding gene: 182681518

Following gene: 182681503

Centisome position: 44.35

GC content: 51.75

Gene sequence:

>1857_bases
GTGCCGCGCCGCCGCTTCGTCCCGTCACTCTCTGGACGAACCATCGTGAACATCACCACTGCCCTCCCCCTTCCCACCCA
CTTGCTGTCGGAGAAGGTGACTGCACCTTTACTTGGCGCACGCAAAATTTACGTTACCGGATCACGCCAAGATATACGTG
TGCCAATGCGCGAAATCGCACTCACACCCAGCTCAGCTCGATACGGCGGAAACGAGAATCTATCACTGGTGCTCTATGAC
ACTTCGGGAATTTACACTGATCCGCAAGCTACGATTGACTTAGCGTGTGGTCTACCTCGATTACGCACTGCTTGGATTGA
TGAGCGTGCCGATACCGTAGAAGCTAAGTTGCATTTCAAAGTACCTGAGTCGGTATCTGTCACCGCACCGCCTTTTCCTA
CACCGCCACAGCCACTGCGTGCACGTGACAATGTCGCGGTAACGCAACTGGAATATGCACGACGTGGTCTCGTCACTCCA
GAAATGGAGTTTGTCGCTATCCGTGAGCAACAACGCCGTGAACAAACTGTCGAAAATCTTCGCGGCCAGCGCCATGCGGG
TGATGCCTGGGGGGCTTTGGTTGGCACACCCATCACTCCTGAATTCGTACGTGATGAAATCGCGCGTGGCCGGGCCATTC
TTCCTAATAACATCAACCATCCTGAAAGCGAGCCAATGATTATTGGCCGCAACTTTTTGACAAAAATCAATGCCAATATT
GGAACATCAGCCCTGTCTTCCAGTATTGCCGAAGAAGTGGAGAAGCTGGTTTGGGCGATCCGCTGGGGCGCAGACACAAT
CATGGACCTCTCTACTGGCCGTGACATCCATGCTACTAGAGAATGGATTCTGCGTAATTCACCTGTTCCTGTGGGGACGG
TGCCAATTTATCAAGCGTTGGAGAAAGTCGATGGACATGTTGATGCTCTGAGTTGGGACGTGTTTCGTGACACTTTGATC
GAACAGGCCGAACAAGGTGTGGACTATGTCACCGTGCATGCAGGAGTCCTTCGAGACTTTATCCCACTGACTGCTTCACG
TTTAACAGGAATCGTCTCGCGTGGCGGTTCCATCATGGCTAGATGGTGTCAGGCACACCGCAGCGAAAACTTCCTATACA
CGCATTTCGAGGAACTCTGTGAAATCATGCGTGCCTACGACGTGGCATTCTCTCTGGGTGATGGATTGCGCCCAGGCTGC
ATCGCTGATGCGAATGATGCAGCTCAATTTGCTGAATTGGAGATTCTTGGCAAGTTGACCCACATCGCCTGGAATCACCA
AGTACAGGTGATGGTAGAAGGTCCTGGACACGTCCCAATGCAGCTAATCAAGGCCAATATGGATAAGCAATTAGCCGCCT
GTGGTGAGGCACCTTTCTACACGCTAGGTCCATTAACTACAGATATCGCCCCAGGTTACGACCACATTACTAGTGCAATT
GGCGCGGCAATGATCGGCTGGTATGGGACAGCGATGCTATGTTACGTGACTCCCAAGGAGCACCTTGGCCTGCCTAATTT
GCAAGATGTCCATGATGGCATCATCGCCTACAAAATTGCGGCACATGCGGCTGATCTTGCCAAAGGGCATCCAGCGGCAC
AGGCACGTGACGATGCACTCTCTAAAGCACGCTTTGAATTCCGCTGGCAAGATCAATTCCATCTCAGCTTGGATCCAGAA
AAAGCACTGGCACTGCATGATGAGAGCCTCCCTAAAGAAGCGCATAAACGAGCAGCATTCTGTTCGATGTGCGGACCGCA
ATTTTGTTCAATGAAAATCTCTCAAGAGGTCCGTGACGCTTCCTCCAGCGAATTGTCAGATGCAAATACGGATATCAATA
CCAGCGACGGCATCTAG

Upstream 100 bases:

>100_bases
CAAGCTGCGGTTGAGACACACCCTTCGAACCTGATCCGGTTTACACCGGCGTAGGAAAGCTTCGTGGACCGCGCCGCATG
GCCACCCTGGCAGGCGTAAG

Downstream 100 bases:

>100_bases
CATTCACTGTGTCTTTGGCATCGGAACGAATGATCTTTTCCGGTGTCAATCACTTTATGTTCTAGATTGACTCTCATTGA
TGTTCATTACACTAAAATTT

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase; HMP-P synthase; HMP-phosphate synthase; HMPP synthase; Thiamine biosynthesis protein thiC [H]

Number of amino acids: Translated: 618; Mature: 617

Protein sequence:

>618_residues
MPRRRFVPSLSGRTIVNITTALPLPTHLLSEKVTAPLLGARKIYVTGSRQDIRVPMREIALTPSSARYGGNENLSLVLYD
TSGIYTDPQATIDLACGLPRLRTAWIDERADTVEAKLHFKVPESVSVTAPPFPTPPQPLRARDNVAVTQLEYARRGLVTP
EMEFVAIREQQRREQTVENLRGQRHAGDAWGALVGTPITPEFVRDEIARGRAILPNNINHPESEPMIIGRNFLTKINANI
GTSALSSSIAEEVEKLVWAIRWGADTIMDLSTGRDIHATREWILRNSPVPVGTVPIYQALEKVDGHVDALSWDVFRDTLI
EQAEQGVDYVTVHAGVLRDFIPLTASRLTGIVSRGGSIMARWCQAHRSENFLYTHFEELCEIMRAYDVAFSLGDGLRPGC
IADANDAAQFAELEILGKLTHIAWNHQVQVMVEGPGHVPMQLIKANMDKQLAACGEAPFYTLGPLTTDIAPGYDHITSAI
GAAMIGWYGTAMLCYVTPKEHLGLPNLQDVHDGIIAYKIAAHAADLAKGHPAAQARDDALSKARFEFRWQDQFHLSLDPE
KALALHDESLPKEAHKRAAFCSMCGPQFCSMKISQEVRDASSSELSDANTDINTSDGI

Sequences:

>Translated_618_residues
MPRRRFVPSLSGRTIVNITTALPLPTHLLSEKVTAPLLGARKIYVTGSRQDIRVPMREIALTPSSARYGGNENLSLVLYD
TSGIYTDPQATIDLACGLPRLRTAWIDERADTVEAKLHFKVPESVSVTAPPFPTPPQPLRARDNVAVTQLEYARRGLVTP
EMEFVAIREQQRREQTVENLRGQRHAGDAWGALVGTPITPEFVRDEIARGRAILPNNINHPESEPMIIGRNFLTKINANI
GTSALSSSIAEEVEKLVWAIRWGADTIMDLSTGRDIHATREWILRNSPVPVGTVPIYQALEKVDGHVDALSWDVFRDTLI
EQAEQGVDYVTVHAGVLRDFIPLTASRLTGIVSRGGSIMARWCQAHRSENFLYTHFEELCEIMRAYDVAFSLGDGLRPGC
IADANDAAQFAELEILGKLTHIAWNHQVQVMVEGPGHVPMQLIKANMDKQLAACGEAPFYTLGPLTTDIAPGYDHITSAI
GAAMIGWYGTAMLCYVTPKEHLGLPNLQDVHDGIIAYKIAAHAADLAKGHPAAQARDDALSKARFEFRWQDQFHLSLDPE
KALALHDESLPKEAHKRAAFCSMCGPQFCSMKISQEVRDASSSELSDANTDINTSDGI
>Mature_617_residues
PRRRFVPSLSGRTIVNITTALPLPTHLLSEKVTAPLLGARKIYVTGSRQDIRVPMREIALTPSSARYGGNENLSLVLYDT
SGIYTDPQATIDLACGLPRLRTAWIDERADTVEAKLHFKVPESVSVTAPPFPTPPQPLRARDNVAVTQLEYARRGLVTPE
MEFVAIREQQRREQTVENLRGQRHAGDAWGALVGTPITPEFVRDEIARGRAILPNNINHPESEPMIIGRNFLTKINANIG
TSALSSSIAEEVEKLVWAIRWGADTIMDLSTGRDIHATREWILRNSPVPVGTVPIYQALEKVDGHVDALSWDVFRDTLIE
QAEQGVDYVTVHAGVLRDFIPLTASRLTGIVSRGGSIMARWCQAHRSENFLYTHFEELCEIMRAYDVAFSLGDGLRPGCI
ADANDAAQFAELEILGKLTHIAWNHQVQVMVEGPGHVPMQLIKANMDKQLAACGEAPFYTLGPLTTDIAPGYDHITSAIG
AAMIGWYGTAMLCYVTPKEHLGLPNLQDVHDGIIAYKIAAHAADLAKGHPAAQARDDALSKARFEFRWQDQFHLSLDPEK
ALALHDESLPKEAHKRAAFCSMCGPQFCSMKISQEVRDASSSELSDANTDINTSDGI

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction [H]

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family [H]

Homologues:

Organism=Escherichia coli, GI1790427, Length=575, Percent_Identity=64.8695652173913, Blast_Score=776, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002817 [H]

Pfam domain/function: PF01964 ThiC [H]

EC number: NA

Molecular weight: Translated: 68066; Mature: 67935

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRRRFVPSLSGRTIVNITTALPLPTHLLSEKVTAPLLGARKIYVTGSRQDIRVPMREIA
CCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHCCCCCCCEEEEEECCCCCCCCCHHHHE
LTPSSARYGGNENLSLVLYDTSGIYTDPQATIDLACGLPRLRTAWIDERADTVEAKLHFK
ECCCCCCCCCCCCEEEEEEECCCCEECCCCCEEECCCCCHHHHHHHHCCCCEEEEEEEEE
VPESVSVTAPPFPTPPQPLRARDNVAVTQLEYARRGLVTPEMEFVAIREQQRREQTVENL
CCCCCEECCCCCCCCCCCCCCCCCEEHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
RGQRHAGDAWGALVGTPITPEFVRDEIARGRAILPNNINHPESEPMIIGRNFLTKINANI
CCCCCCCCHHHHHCCCCCCHHHHHHHHHCCCEECCCCCCCCCCCCEEECCHHHHHCCCCC
GTSALSSSIAEEVEKLVWAIRWGADTIMDLSTGRDIHATREWILRNSPVPVGTVPIYQAL
CHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHHCCCCCCCCCCHHHHHH
EKVDGHVDALSWDVFRDTLIEQAEQGVDYVTVHAGVLRDFIPLTASRLTGIVSRGGSIMA
HHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCHHHHH
RWCQAHRSENFLYTHFEELCEIMRAYDVAFSLGDGLRPGCIADANDAAQFAELEILGKLT
HHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
HIAWNHQVQVMVEGPGHVPMQLIKANMDKQLAACGEAPFYTLGPLTTDIAPGYDHITSAI
HEEECCEEEEEEECCCCCHHHHHHHCHHHHHHHCCCCCCEEECCCCCCCCCCHHHHHHHH
GAAMIGWYGTAMLCYVTPKEHLGLPNLQDVHDGIIAYKIAAHAADLAKGHPAAQARDDAL
HHHHHHHHCCEEEEEECCHHHCCCCCHHHHHCCHHHHHHHHHHHHHHCCCCCCHHHHHHH
SKARFEFRWQDQFHLSLDPEKALALHDESLPKEAHKRAAFCSMCGPQFCSMKISQEVRDA
HHHHEEEEECCCEEEEECHHHHHEECCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCC
SSSELSDANTDINTSDGI
CCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PRRRFVPSLSGRTIVNITTALPLPTHLLSEKVTAPLLGARKIYVTGSRQDIRVPMREIA
CCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHCCCCCCCEEEEEECCCCCCCCCHHHHE
LTPSSARYGGNENLSLVLYDTSGIYTDPQATIDLACGLPRLRTAWIDERADTVEAKLHFK
ECCCCCCCCCCCCEEEEEEECCCCEECCCCCEEECCCCCHHHHHHHHCCCCEEEEEEEEE
VPESVSVTAPPFPTPPQPLRARDNVAVTQLEYARRGLVTPEMEFVAIREQQRREQTVENL
CCCCCEECCCCCCCCCCCCCCCCCEEHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
RGQRHAGDAWGALVGTPITPEFVRDEIARGRAILPNNINHPESEPMIIGRNFLTKINANI
CCCCCCCCHHHHHCCCCCCHHHHHHHHHCCCEECCCCCCCCCCCCEEECCHHHHHCCCCC
GTSALSSSIAEEVEKLVWAIRWGADTIMDLSTGRDIHATREWILRNSPVPVGTVPIYQAL
CHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHHCCCCCCCCCCHHHHHH
EKVDGHVDALSWDVFRDTLIEQAEQGVDYVTVHAGVLRDFIPLTASRLTGIVSRGGSIMA
HHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCHHHHH
RWCQAHRSENFLYTHFEELCEIMRAYDVAFSLGDGLRPGCIADANDAAQFAELEILGKLT
HHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
HIAWNHQVQVMVEGPGHVPMQLIKANMDKQLAACGEAPFYTLGPLTTDIAPGYDHITSAI
HEEECCEEEEEEECCCCCHHHHHHHCHHHHHHHCCCCCCEEECCCCCCCCCCHHHHHHHH
GAAMIGWYGTAMLCYVTPKEHLGLPNLQDVHDGIIAYKIAAHAADLAKGHPAAQARDDAL
HHHHHHHHCCEEEEEECCHHHCCCCCHHHHHCCHHHHHHHHHHHHHHCCCCCCHHHHHHH
SKARFEFRWQDQFHLSLDPEKALALHDESLPKEAHKRAAFCSMCGPQFCSMKISQEVRDA
HHHHEEEEECCCEEEEECHHHHHEECCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCC
SSSELSDANTDINTSDGI
CCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12533478 [H]