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The map label for this gene is rph

Identifier: 182681311

GI number: 182681311

Start: 894977

End: 895702

Strand: Direct

Name: rph

Synonym: XfasM23_0758

Alternate gene names: 182681311

Gene position: 894977-895702 (Clockwise)

Preceding gene: 182681307

Following gene: 182681312

Centisome position: 35.3

GC content: 56.34

Gene sequence:

>726_bases
ATGAATGTTTCACGTCCCAGTGGTCGTCAAGCCGATGCGTTGCGACCGGTGCGTATTGAGCGCGCCTTTACCTGCCATGC
TGAAGGCTCAGTTCTAGTCAGTTTTGGTAACACCTTGGTGGTGTGCACTGCAAGTGTGGAGGCCAAAGTGCCCGTGTTTC
TGCGCAATAAGGGGGAAGGGTGGATCACTGCCGAATATGGAATGCTCCCACGTTCCACCCATACGCGTTCCGAGCGCGAG
GCCGCACGTGGTAAACAGGCAGGGCGTACCTTGGAGATTCAGCGTCTGATTGGACGTGCACTGCGCACCTGTGTCGATCG
CACTGCATTGGGCGAGCGCACGATTACCTTGGATTGTGATGTGTTACAGGCAGATGGCGGCACCCGTACCGCAGCGATCA
CGGGTGCTTATGTTGCCTTGGTCGATGCGGTGCGTTGTTTAGAGCAGCGAGGTCAATTGAAGAAGTCACCACTGATTGGT
GCTGTAGCGGCTGTCTCGGTTGGCATTTACCGTGGTATGCCAGTCTTGGACCTGGATTACCCTGAGGACAGTGACTGCGA
CACTGATATGAACGTCGTCATGAACGATGAGGGTGGTTTTATCGAGTTGCAGGGGACCGCTGAGCAGCAGGCATTCCGCC
GCGGTGAACTGGACATGTTATTGGCGTTGGCTGAGCGCGGCACCGCCATGCTATTTGACATCCAGCGTGAAGCGTTGGCC
CGCTAG

Upstream 100 bases:

>100_bases
CATATTGCTCTCAAAATATGGATGGTACCCTAGCGCGCCCATGCGGGGCCTTAAGGTGGTTCAATTTTTCATTCACTATG
ACTAATAAGAAAAAACCAAT

Downstream 100 bases:

>100_bases
AATCGCAGATCTGTTGGGCGCCACATGCATTGCAGTGATGATTGACGATGGAGCCATCCACGGGTAATGCATCAATATGA
TTGGAACCGAAGATGATGAA

Product: ribonuclease PH

Products: NA

Alternate protein names: RNase PH; tRNA nucleotidyltransferase

Number of amino acids: Translated: 241; Mature: 241

Protein sequence:

>241_residues
MNVSRPSGRQADALRPVRIERAFTCHAEGSVLVSFGNTLVVCTASVEAKVPVFLRNKGEGWITAEYGMLPRSTHTRSERE
AARGKQAGRTLEIQRLIGRALRTCVDRTALGERTITLDCDVLQADGGTRTAAITGAYVALVDAVRCLEQRGQLKKSPLIG
AVAAVSVGIYRGMPVLDLDYPEDSDCDTDMNVVMNDEGGFIELQGTAEQQAFRRGELDMLLALAERGTAMLFDIQREALA
R

Sequences:

>Translated_241_residues
MNVSRPSGRQADALRPVRIERAFTCHAEGSVLVSFGNTLVVCTASVEAKVPVFLRNKGEGWITAEYGMLPRSTHTRSERE
AARGKQAGRTLEIQRLIGRALRTCVDRTALGERTITLDCDVLQADGGTRTAAITGAYVALVDAVRCLEQRGQLKKSPLIG
AVAAVSVGIYRGMPVLDLDYPEDSDCDTDMNVVMNDEGGFIELQGTAEQQAFRRGELDMLLALAERGTAMLFDIQREALA
R
>Mature_241_residues
MNVSRPSGRQADALRPVRIERAFTCHAEGSVLVSFGNTLVVCTASVEAKVPVFLRNKGEGWITAEYGMLPRSTHTRSERE
AARGKQAGRTLEIQRLIGRALRTCVDRTALGERTITLDCDVLQADGGTRTAAITGAYVALVDAVRCLEQRGQLKKSPLIG
AVAAVSVGIYRGMPVLDLDYPEDSDCDTDMNVVMNDEGGFIELQGTAEQQAFRRGELDMLLALAERGTAMLFDIQREALA
R

Specific function: Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates

COG id: COG0689

COG function: function code J; RNase PH

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase PH family

Homologues:

Organism=Escherichia coli, GI157672248, Length=208, Percent_Identity=67.3076923076923, Blast_Score=282, Evalue=2e-77,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNPH_XYLF2 (B2IA38)

Other databases:

- EMBL:   CP001011
- RefSeq:   YP_001829471.1
- ProteinModelPortal:   B2IA38
- SMR:   B2IA38
- GeneID:   6202919
- GenomeReviews:   CP001011_GR
- KEGG:   xfn:XfasM23_0758
- HOGENOM:   HBG737187
- OMA:   MLPRATG
- ProtClustDB:   PRK00173
- HAMAP:   MF_00564
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR020568
- InterPro:   IPR002381
- InterPro:   IPR018336
- TIGRFAMs:   TIGR01966

Pfam domain/function: PF01138 RNase_PH; PF03725 RNase_PH_C; SSF55666 3_ExoRNase; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.56

Molecular weight: Translated: 26215; Mature: 26215

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: PS01277 RIBONUCLEASE_PH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVSRPSGRQADALRPVRIERAFTCHAEGSVLVSFGNTLVVCTASVEAKVPVFLRNKGEG
CCCCCCCCCCCCCCCCEEEEEEEEEECCCCEEEEECCEEEEEEECCCCCCEEEEEECCCE
WITAEYGMLPRSTHTRSEREAARGKQAGRTLEIQRLIGRALRTCVDRTALGERTITLDCD
EEEEECCCCCCCCCCCHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHCCCEEEEEEEE
VLQADGGTRTAAITGAYVALVDAVRCLEQRGQLKKSPLIGAVAAVSVGIYRGMPVLDLDY
EEECCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCEEECCC
PEDSDCDTDMNVVMNDEGGFIELQGTAEQQAFRRGELDMLLALAERGTAMLFDIQREALA
CCCCCCCCCCEEEECCCCCEEEEECCHHHHHHHCCCHHHHHHHHHCCCEEEEEECHHHHC
R
C
>Mature Secondary Structure
MNVSRPSGRQADALRPVRIERAFTCHAEGSVLVSFGNTLVVCTASVEAKVPVFLRNKGEG
CCCCCCCCCCCCCCCCEEEEEEEEEECCCCEEEEECCEEEEEEECCCCCCEEEEEECCCE
WITAEYGMLPRSTHTRSEREAARGKQAGRTLEIQRLIGRALRTCVDRTALGERTITLDCD
EEEEECCCCCCCCCCCHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHCCCEEEEEEEE
VLQADGGTRTAAITGAYVALVDAVRCLEQRGQLKKSPLIGAVAAVSVGIYRGMPVLDLDY
EEECCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCEEECCC
PEDSDCDTDMNVVMNDEGGFIELQGTAEQQAFRRGELDMLLALAERGTAMLFDIQREALA
CCCCCCCCCCEEEECCCCCEEEEECCHHHHHHHCCCHHHHHHHHHCCCEEEEEECHHHHC
R
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA