Click here to switch to the map view.

The map label for this gene is ubiE

Identifier: 182681294

GI number: 182681294

Start: 871197

End: 871958

Strand: Direct

Name: ubiE

Synonym: XfasM23_0740

Alternate gene names: 182681294

Gene position: 871197-871958 (Clockwise)

Preceding gene: 182681293

Following gene: 182681295

Centisome position: 34.36

GC content: 53.15

Gene sequence:

>762_bases
ATGAGTGAATCTTCCGAGAAAACCAGCACGACCCATTTCGGCTTCCGTCAGGTTGCGGCCAAGGACAAGAAGACGCTGGT
TGCCGAGGTGTTTACTTCGGTCTCACGTCGCTATGACTTGATGAACGATCTGATGAGCCTGGGTATTCATCGGGCCTGGA
AGCGTTACTTCGTCGCTACTGCTCAGGTGAAGTCAGGTGACCGTGTGCTTGACCTTGCAGGGGGCACCGGCGATATCGCA
ATGCTATTGAAGAATCGGGTTGGTGCAGAGGGGAGTATCGTACTTGGCGATATCAATGCCAGCATGCTGTCCGTTGGGCG
GGATAGGCTGATTGATCGTGGCGTGGTCGCTCGTCTGGACTATGTGCAATGCAACGCTGAAGCGCTGCCGTTTCAGGATA
AGTGCTTCGACTTGGTGACGATGTCTTTTGGTTTGCGCAATGTGACCGACAAAGATGCAGCGCTGCGCGAGATGTTCCGT
GTGCTGAAGGTGGGTGGCCAGGCAAGGGTACTGGAATTCTCCGCAGTAACTGCCGAGTGGTTTAAGCCAATTTATGATTT
CCATTCCTTCCAGGTGTTACCGCGTCTGGGGCGGTTGTTTGCTAGGGATGCTGCTAGCTATCGTTACCTTGCTGAGAGCA
TTCGGAAGCATCCACCGCAGGAAGAGTTGCAGGCGATGATGGGGTCGGCTGGGTTTGAGCGTTGTGGCTATAGGAATTTG
ACCGGTGGCATTGTTGCGATTCATTCCGGATATAAATATTGA

Upstream 100 bases:

>100_bases
ATGGGTTTGATGTGGATTTGACTGTATCTGGAAGTTTGGAACACTAATTAATATATGGCCTTCTCAGGCTTATGATGGCG
GTGCAATAATCGGGGTCAAT

Downstream 100 bases:

>100_bases
TGTTGCTTCGTGATGCTCATTGGCGCGAGATGAATCTAGGATGCGTGGCGAATGCAGATCGTTGATGTAGATGGGGGTGA
GGCAGAAAATTGCGCTTGAG

Product: ubiquinone/menaquinone biosynthesis methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 253; Mature: 252

Protein sequence:

>253_residues
MSESSEKTSTTHFGFRQVAAKDKKTLVAEVFTSVSRRYDLMNDLMSLGIHRAWKRYFVATAQVKSGDRVLDLAGGTGDIA
MLLKNRVGAEGSIVLGDINASMLSVGRDRLIDRGVVARLDYVQCNAEALPFQDKCFDLVTMSFGLRNVTDKDAALREMFR
VLKVGGQARVLEFSAVTAEWFKPIYDFHSFQVLPRLGRLFARDAASYRYLAESIRKHPPQEELQAMMGSAGFERCGYRNL
TGGIVAIHSGYKY

Sequences:

>Translated_253_residues
MSESSEKTSTTHFGFRQVAAKDKKTLVAEVFTSVSRRYDLMNDLMSLGIHRAWKRYFVATAQVKSGDRVLDLAGGTGDIA
MLLKNRVGAEGSIVLGDINASMLSVGRDRLIDRGVVARLDYVQCNAEALPFQDKCFDLVTMSFGLRNVTDKDAALREMFR
VLKVGGQARVLEFSAVTAEWFKPIYDFHSFQVLPRLGRLFARDAASYRYLAESIRKHPPQEELQAMMGSAGFERCGYRNL
TGGIVAIHSGYKY
>Mature_252_residues
SESSEKTSTTHFGFRQVAAKDKKTLVAEVFTSVSRRYDLMNDLMSLGIHRAWKRYFVATAQVKSGDRVLDLAGGTGDIAM
LLKNRVGAEGSIVLGDINASMLSVGRDRLIDRGVVARLDYVQCNAEALPFQDKCFDLVTMSFGLRNVTDKDAALREMFRV
LKVGGQARVLEFSAVTAEWFKPIYDFHSFQVLPRLGRLFARDAASYRYLAESIRKHPPQEELQAMMGSAGFERCGYRNLT
GGIVAIHSGYKY

Specific function: Methyltransferase required for the conversion of dimethylmenaquinone (DMKH2) to menaquinone (MKH2) and the conversion of 2-polyprenyl-6-methoxy-1,4-benzoquinol (DDMQH2) to 2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinol (DMQH2)

COG id: COG2226

COG function: function code H; Methylase involved in ubiquinone/menaquinone biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. UbiE family

Homologues:

Organism=Homo sapiens, GI116063536, Length=280, Percent_Identity=43.5714285714286, Blast_Score=224, Evalue=7e-59,
Organism=Escherichia coli, GI48994983, Length=244, Percent_Identity=59.0163934426229, Blast_Score=296, Evalue=6e-82,
Organism=Caenorhabditis elegans, GI17557039, Length=253, Percent_Identity=47.0355731225296, Blast_Score=231, Evalue=2e-61,
Organism=Saccharomyces cerevisiae, GI6323526, Length=262, Percent_Identity=41.9847328244275, Blast_Score=178, Evalue=6e-46,
Organism=Drosophila melanogaster, GI24641702, Length=261, Percent_Identity=45.2107279693487, Blast_Score=217, Evalue=7e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): UBIE_XYLF2 (B2IA21)

Other databases:

- EMBL:   CP001011
- RefSeq:   YP_001829454.1
- ProteinModelPortal:   B2IA21
- SMR:   B2IA21
- GeneID:   6203656
- GenomeReviews:   CP001011_GR
- KEGG:   xfn:XfasM23_0740
- HOGENOM:   HBG559580
- OMA:   FDVYTIA
- ProtClustDB:   PRK00216
- HAMAP:   MF_01813
- InterPro:   IPR004033
- PANTHER:   PTHR10108:SF24
- TIGRFAMs:   TIGR01934

Pfam domain/function: PF01209 Ubie_methyltran

EC number: 2.1.1.-

Molecular weight: Translated: 28255; Mature: 28124

Theoretical pI: Translated: 9.66; Mature: 9.66

Prosite motif: PS01183 UBIE_1; PS01184 UBIE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCEEEECCCCCC
>Mature Secondary Structure 
SESSEKTSTTHFGFRQVAAKDKKTLVAEVFTSVSRRYDLMNDLMSLGIHRAWKRYFVAT
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AQVKSGDRVLDLAGGTGDIAMLLKNRVGAEGSIVLGDINASMLSVGRDRLIDRGVVARLD
EEECCCCEEEEECCCCCHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHH
YVQCNAEALPFQDKCFDLVTMSFGLRNVTDKDAALREMFRVLKVGGQARVLEFSAVTAEW
HEEECCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCEEEEEHHHHHHHH
FKPIYDFHSFQVLPRLGRLFARDAASYRYLAESIRKHPPQEELQAMMGSAGFERCGYRNL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHCCCCCC
TGGIVAIHSGYKY
CCCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring one-carbon groups; Methyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA