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The map label for this gene is yfjR [H]

Identifier: 182680482

GI number: 182680482

Start: 4062627

End: 4063529

Strand: Reverse

Name: yfjR [H]

Synonym: Bind_3583

Alternate gene names: 182680482

Gene position: 4063529-4062627 (Counterclockwise)

Preceding gene: 182680483

Following gene: 182680481

Centisome position: 97.44

GC content: 61.13

Gene sequence:

>903_bases
ATGCAACTCGGATTTTTAGGTCTTGGCCATATGGGCCTAGCCATCGCTTCGAACCTTTTGGCCGCCGGTCATCATGTCCG
AGCCTGGAACCGCTCACAGGAACCCTTGGCTCGCTTGCAGGCAAAGGGCGCTGAAATCGTCAAGGAGCCCGTCGACGCCC
TGCAGGGCGATGCCCTGTTTTCGATGTTGGCGAATGACGACGCCGTCCGTTCCGTGCTCATAGATTCGGGTGCGCTCGCC
CAAGCGCGCAAGGGGCTGATTCACGTCAATATGGCGACGATCTCGGTGGCCCTCGCCGAGGAACTGGCCGTGCTCCACGA
TAGACTCGGCCTTGCTTATGTCGCAGCCCCCGTTTTCGGCCGTCCGGAGGCCGCTGCTTCCGCCAAGCTGCATATTGTCG
TTGCCGGCGCACCGAGCCCTGTCGCTTCCATCGAGCCGGTACTGGCCGCCCTCGGTCAGAAAATCTGGCCCGTGGGCAGC
GAGCCGAAAGCGGCCAATATTGTGAAAATCGCCGGTAATTTCATGATCGCATCGATGATCGAGACGATCGGCGAGGCTGC
GGCCCTGGTCCAGGGCCATGGGGTGACGGCAGCCGATTTTCTCGACGTCATGACCAATAGTCTCTTTGCCGCTCCGGCCT
ATAAAACCTATGGCGATTTGATCGCGAGGGCTCAATTCGAGCCAGCCGGGTTCAAACTCACTCTAGGCCTCAAGGATGTT
CGCCTGGCCTTGGCTGCGGGAGAAGCCGCCCATGTCCCCTTGCCGCTAGGGAGTCTGCTGCGAGATAATTTCCTCGATTG
CATCGCGCATGGCGAAAGCGACAAGGACTGGTCGGCGCTTGCGGGCGTCGCCGCCCGGCGCGCGGGCCTTGATGAGCGTG
ACGAAAAGCGCCCCATTGCCTAG

Upstream 100 bases:

>100_bases
CCTTGGCCTATGAGAAGCGCTCGTCTGGATTACAGGGCGAATTTAGCTGGTGCGTTTGACAACCACCCCGCCCTCAAAAA
CGGGGAGGAGGATCTTGGCA

Downstream 100 bases:

>100_bases
GACCGGCCAAAAAGCCTATAAGACCGACGGCATATTCATCCGACACCCCTGGAGGCGTGGTGTGCCGCTTCTTCTCAAAA
TCTAACGCCAACATGATCGG

Product: 6-phosphogluconate dehydrogenase

Products: (R)-Glycerate; Nicotinamide adenine dinucleotide [C]

Alternate protein names: NA

Number of amino acids: Translated: 300; Mature: 300

Protein sequence:

>300_residues
MQLGFLGLGHMGLAIASNLLAAGHHVRAWNRSQEPLARLQAKGAEIVKEPVDALQGDALFSMLANDDAVRSVLIDSGALA
QARKGLIHVNMATISVALAEELAVLHDRLGLAYVAAPVFGRPEAAASAKLHIVVAGAPSPVASIEPVLAALGQKIWPVGS
EPKAANIVKIAGNFMIASMIETIGEAAALVQGHGVTAADFLDVMTNSLFAAPAYKTYGDLIARAQFEPAGFKLTLGLKDV
RLALAAGEAAHVPLPLGSLLRDNFLDCIAHGESDKDWSALAGVAARRAGLDERDEKRPIA

Sequences:

>Translated_300_residues
MQLGFLGLGHMGLAIASNLLAAGHHVRAWNRSQEPLARLQAKGAEIVKEPVDALQGDALFSMLANDDAVRSVLIDSGALA
QARKGLIHVNMATISVALAEELAVLHDRLGLAYVAAPVFGRPEAAASAKLHIVVAGAPSPVASIEPVLAALGQKIWPVGS
EPKAANIVKIAGNFMIASMIETIGEAAALVQGHGVTAADFLDVMTNSLFAAPAYKTYGDLIARAQFEPAGFKLTLGLKDV
RLALAAGEAAHVPLPLGSLLRDNFLDCIAHGESDKDWSALAGVAARRAGLDERDEKRPIA
>Mature_300_residues
MQLGFLGLGHMGLAIASNLLAAGHHVRAWNRSQEPLARLQAKGAEIVKEPVDALQGDALFSMLANDDAVRSVLIDSGALA
QARKGLIHVNMATISVALAEELAVLHDRLGLAYVAAPVFGRPEAAASAKLHIVVAGAPSPVASIEPVLAALGQKIWPVGS
EPKAANIVKIAGNFMIASMIETIGEAAALVQGHGVTAADFLDVMTNSLFAAPAYKTYGDLIARAQFEPAGFKLTLGLKDV
RLALAAGEAAHVPLPLGSLLRDNFLDCIAHGESDKDWSALAGVAARRAGLDERDEKRPIA

Specific function: Glyoxylate catabolism. [C]

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI40556376, Length=281, Percent_Identity=30.6049822064057, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI23308751, Length=288, Percent_Identity=27.7777777777778, Blast_Score=95, Evalue=8e-20,
Organism=Escherichia coli, GI1786719, Length=283, Percent_Identity=30.3886925795053, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI1790315, Length=283, Percent_Identity=30.0353356890459, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI145693186, Length=283, Percent_Identity=27.5618374558304, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1789092, Length=283, Percent_Identity=26.8551236749117, Blast_Score=72, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17557316, Length=265, Percent_Identity=24.9056603773585, Blast_Score=64, Evalue=9e-11,
Organism=Drosophila melanogaster, GI24655230, Length=286, Percent_Identity=29.020979020979, Blast_Score=99, Evalue=3e-21,
Organism=Drosophila melanogaster, GI19922568, Length=286, Percent_Identity=29.020979020979, Blast_Score=99, Evalue=3e-21,
Organism=Drosophila melanogaster, GI28574115, Length=285, Percent_Identity=24.2105263157895, Blast_Score=84, Evalue=9e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR016040 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: 1.1.1.60 [C]

Molecular weight: Translated: 31257; Mature: 31257

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLGFLGLGHMGLAIASNLLAAGHHVRAWNRSQEPLARLQAKGAEIVKEPVDALQGDALF
CCCCCEECCHHHHHHHHHHHHCCCHHCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHH
SMLANDDAVRSVLIDSGALAQARKGLIHVNMATISVALAEELAVLHDRLGLAYVAAPVFG
HHHHCCHHHHHHHHCCCHHHHHHCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
RPEAAASAKLHIVVAGAPSPVASIEPVLAALGQKIWPVGSEPKAANIVKIAGNFMIASMI
CCCCCCCCEEEEEEECCCCCHHHHHHHHHHCCHHCCCCCCCCCCCCEEEEHHHHHHHHHH
ETIGEAAALVQGHGVTAADFLDVMTNSLFAAPAYKTYGDLIARAQFEPAGFKLTLGLKDV
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCEEEEECHHHH
RLALAAGEAAHVPLPLGSLLRDNFLDCIAHGESDKDWSALAGVAARRAGLDERDEKRPIA
HHHEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MQLGFLGLGHMGLAIASNLLAAGHHVRAWNRSQEPLARLQAKGAEIVKEPVDALQGDALF
CCCCCEECCHHHHHHHHHHHHCCCHHCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHH
SMLANDDAVRSVLIDSGALAQARKGLIHVNMATISVALAEELAVLHDRLGLAYVAAPVFG
HHHHCCHHHHHHHHCCCHHHHHHCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
RPEAAASAKLHIVVAGAPSPVASIEPVLAALGQKIWPVGSEPKAANIVKIAGNFMIASMI
CCCCCCCCEEEEEEECCCCCHHHHHHHHHHCCHHCCCCCCCCCCCCEEEEHHHHHHHHHH
ETIGEAAALVQGHGVTAADFLDVMTNSLFAAPAYKTYGDLIARAQFEPAGFKLTLGLKDV
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCEEEEECHHHH
RLALAAGEAAHVPLPLGSLLRDNFLDCIAHGESDKDWSALAGVAARRAGLDERDEKRPIA
HHHEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-Hydroxy-3-oxopropanoate; H+; Nicotinamide adenine dinucleotide - reduced [C]

Specific reaction: 2-Hydroxy-3-oxopropanoate + H+ + Nicotinamide adenine dinucleotide - reduced --> (R)-Glycerate + Nicotinamide adenine dinucleotide [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]