Click here to switch to the map view.

The map label for this gene is pgk [H]

Identifier: 182680367

GI number: 182680367

Start: 3942098

End: 3943327

Strand: Reverse

Name: pgk [H]

Synonym: Bind_3467

Alternate gene names: 182680367

Gene position: 3943327-3942098 (Counterclockwise)

Preceding gene: 182680368

Following gene: 182680366

Centisome position: 94.56

GC content: 60.73

Gene sequence:

>1230_bases
ATGACCAGAACCGTGCCAGCCTCGCCTTTCCCCACCCTCGACGATGTCGTCGTCACCGGCAAGCGCGTCCTCGTGCGGGT
CGATCTCAACGTTCCGATGGATAATGGCATCATCACCGATGCCACGCGCATCGACCGTATCCTGCCCAATATCAAGGAAA
TCTCGCAGAAGGGCGGCAAGGTCATCCTGTTGTCCCATCTCGGCCGGCCGAAGAATGGGCCGGAGGCCAAAACGTCGCTG
AAACCGGTTGCCGTGGAGCTCGAACGGCACCTTGGCCGCTATGTCGCCTTCGCCTCTGATTGCGTCGGCGATGTCGCCAA
AAGCGCTGCCTCCGCCCTCAAGGAGGGGGATATCCTGCTCCTCGAAAATACGCGTTTCCACCCGCAGGAAACCAAGAACG
ACCCGGCTTTTGTTGATGCGCTCGCTGAACTCGGCGATATTTATGTCAATGATGCCTTTTCGACCGCGCATCGTGCACAT
GCGACAACGGAAGGTCTGGCGCATAAACTGCCCGCTTATGCTGGCCGCACCATGCAGCTGGAGCTCGACATGTTGACCAG
CATGCTGGCGCATCCGGAACGGCCGCTCGCGGCCATCGTCGGTGGGGCCAAGGTTTCGACTAAGCTTGAATTGCTCGGCA
ATCTCATGCGCCGCGTCGAATATTTGTTCATCGGCGGCGGCATGGCCAATACATTCCTGGCCGCCAGCGGCAAGCCGATC
GGCAAATCACTTTGCGAGATGGATCTTGCCGATGTCGCACGCAAGATCATGGCCGATGCCGAGGCGGCGCATTGCCAGCT
TGTCCTGCCGGTCGATGCGATCGTCGCCCCCAAGCTGGAGGCCAATATTCCGACCCGGGCCATCGATATCGATGCCATTA
CCGAAAACGAGATGATCCTCGACATCGGCCCGCGCTCGATTTCGAAAATCGTCACCCTGCTGGGCTCCGCCCGCACCCTG
GTCTGGAACGGCCCGGTCGGTGCCTTCGAAGTGCCGCCCTTCCATGTTGGCACGATGGCGATCGCCAAAGTGGCTGCCCA
GCTCACCAAGGTTTCGGCGCTGGAAACCATTGCCGGCGGTGGCGATACGATCGCCGCCCTGAACCAGGCCGGCGCCTATG
ACGATTTCACCTATGTCTCGACAGCCGGAGGCGCCTTCCTCGAATGGCTCGAAGGCAAGCTTCTGCCGGGCGTCGAGGCC
CTGCGCGGCTCGCAGGATTTCACCCTTTAA

Upstream 100 bases:

>100_bases
TTCTGGAAGGCACCTTCGTGCGTATCCTCTCCTGGTATGACAACGAATGGGGCTTTTCCAACCGGATGAGTGATACCGCT
GTCGCAATCGGGAAGCTTAT

Downstream 100 bases:

>100_bases
AAAGAGCCTTCTGATGCAGCCGCATCAGAAGGCTTTCTATTTTGTTTAAATGCGTAACCCTCATCCCCAAAGTTTCCAGC
TTTTGGGGTGCGCCCGAGCA

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 409; Mature: 408

Protein sequence:

>409_residues
MTRTVPASPFPTLDDVVVTGKRVLVRVDLNVPMDNGIITDATRIDRILPNIKEISQKGGKVILLSHLGRPKNGPEAKTSL
KPVAVELERHLGRYVAFASDCVGDVAKSAASALKEGDILLLENTRFHPQETKNDPAFVDALAELGDIYVNDAFSTAHRAH
ATTEGLAHKLPAYAGRTMQLELDMLTSMLAHPERPLAAIVGGAKVSTKLELLGNLMRRVEYLFIGGGMANTFLAASGKPI
GKSLCEMDLADVARKIMADAEAAHCQLVLPVDAIVAPKLEANIPTRAIDIDAITENEMILDIGPRSISKIVTLLGSARTL
VWNGPVGAFEVPPFHVGTMAIAKVAAQLTKVSALETIAGGGDTIAALNQAGAYDDFTYVSTAGGAFLEWLEGKLLPGVEA
LRGSQDFTL

Sequences:

>Translated_409_residues
MTRTVPASPFPTLDDVVVTGKRVLVRVDLNVPMDNGIITDATRIDRILPNIKEISQKGGKVILLSHLGRPKNGPEAKTSL
KPVAVELERHLGRYVAFASDCVGDVAKSAASALKEGDILLLENTRFHPQETKNDPAFVDALAELGDIYVNDAFSTAHRAH
ATTEGLAHKLPAYAGRTMQLELDMLTSMLAHPERPLAAIVGGAKVSTKLELLGNLMRRVEYLFIGGGMANTFLAASGKPI
GKSLCEMDLADVARKIMADAEAAHCQLVLPVDAIVAPKLEANIPTRAIDIDAITENEMILDIGPRSISKIVTLLGSARTL
VWNGPVGAFEVPPFHVGTMAIAKVAAQLTKVSALETIAGGGDTIAALNQAGAYDDFTYVSTAGGAFLEWLEGKLLPGVEA
LRGSQDFTL
>Mature_408_residues
TRTVPASPFPTLDDVVVTGKRVLVRVDLNVPMDNGIITDATRIDRILPNIKEISQKGGKVILLSHLGRPKNGPEAKTSLK
PVAVELERHLGRYVAFASDCVGDVAKSAASALKEGDILLLENTRFHPQETKNDPAFVDALAELGDIYVNDAFSTAHRAHA
TTEGLAHKLPAYAGRTMQLELDMLTSMLAHPERPLAAIVGGAKVSTKLELLGNLMRRVEYLFIGGGMANTFLAASGKPIG
KSLCEMDLADVARKIMADAEAAHCQLVLPVDAIVAPKLEANIPTRAIDIDAITENEMILDIGPRSISKIVTLLGSARTLV
WNGPVGAFEVPPFHVGTMAIAKVAAQLTKVSALETIAGGGDTIAALNQAGAYDDFTYVSTAGGAFLEWLEGKLLPGVEAL
RGSQDFTL

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family [H]

Homologues:

Organism=Homo sapiens, GI4505763, Length=410, Percent_Identity=43.9024390243902, Blast_Score=293, Evalue=2e-79,
Organism=Homo sapiens, GI31543397, Length=411, Percent_Identity=42.8223844282238, Blast_Score=285, Evalue=4e-77,
Organism=Escherichia coli, GI1789294, Length=392, Percent_Identity=41.8367346938776, Blast_Score=285, Evalue=4e-78,
Organism=Caenorhabditis elegans, GI17508823, Length=411, Percent_Identity=41.3625304136253, Blast_Score=291, Evalue=5e-79,
Organism=Saccharomyces cerevisiae, GI6319857, Length=410, Percent_Identity=40.4878048780488, Blast_Score=259, Evalue=4e-70,
Organism=Drosophila melanogaster, GI24581203, Length=410, Percent_Identity=42.1951219512195, Blast_Score=276, Evalue=3e-74,
Organism=Drosophila melanogaster, GI17136394, Length=408, Percent_Identity=40.9313725490196, Blast_Score=269, Evalue=3e-72,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015911
- InterPro:   IPR015824 [H]

Pfam domain/function: PF00162 PGK [H]

EC number: =2.7.2.3 [H]

Molecular weight: Translated: 43470; Mature: 43339

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: PS00111 PGLYCERATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRTVPASPFPTLDDVVVTGKRVLVRVDLNVPMDNGIITDATRIDRILPNIKEISQKGGK
CCCCCCCCCCCCHHHHHCCCCEEEEEEEECCCCCCCEECCHHHHHHHCCCHHHHHHCCCC
VILLSHLGRPKNGPEAKTSLKPVAVELERHLGRYVAFASDCVGDVAKSAASALKEGDILL
EEEEECCCCCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
LENTRFHPQETKNDPAFVDALAELGDIYVNDAFSTAHRAHATTEGLAHKLPAYAGRTMQL
EECCCCCCCCCCCCHHHHHHHHHHHHEEECCHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
ELDMLTSMLAHPERPLAAIVGGAKVSTKLELLGNLMRRVEYLFIGGGMANTFLAASGKPI
EHHHHHHHHCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHEEEECCCHHHHHEECCCCCH
GKSLCEMDLADVARKIMADAEAAHCQLVLPVDAIVAPKLEANIPTRAIDIDAITENEMIL
HHHHHHHHHHHHHHHHHHCCCCCCEEEEEECHHHHCCCCCCCCCCEEEEEEEECCCCEEE
DIGPRSISKIVTLLGSARTLVWNGPVGAFEVPPFHVGTMAIAKVAAQLTKVSALETIAGG
EECHHHHHHHHHHHCCCCEEEECCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
GDTIAALNQAGAYDDFTYVSTAGGAFLEWLEGKLLPGVEALRGSQDFTL
CCHHHHHHCCCCCCCCCEEECCCHHHHHHHCCCCCCCHHHHCCCCCCCC
>Mature Secondary Structure 
TRTVPASPFPTLDDVVVTGKRVLVRVDLNVPMDNGIITDATRIDRILPNIKEISQKGGK
CCCCCCCCCCCHHHHHCCCCEEEEEEEECCCCCCCEECCHHHHHHHCCCHHHHHHCCCC
VILLSHLGRPKNGPEAKTSLKPVAVELERHLGRYVAFASDCVGDVAKSAASALKEGDILL
EEEEECCCCCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
LENTRFHPQETKNDPAFVDALAELGDIYVNDAFSTAHRAHATTEGLAHKLPAYAGRTMQL
EECCCCCCCCCCCCHHHHHHHHHHHHEEECCHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
ELDMLTSMLAHPERPLAAIVGGAKVSTKLELLGNLMRRVEYLFIGGGMANTFLAASGKPI
EHHHHHHHHCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHEEEECCCHHHHHEECCCCCH
GKSLCEMDLADVARKIMADAEAAHCQLVLPVDAIVAPKLEANIPTRAIDIDAITENEMIL
HHHHHHHHHHHHHHHHHHCCCCCCEEEEEECHHHHCCCCCCCCCCEEEEEEEECCCCEEE
DIGPRSISKIVTLLGSARTLVWNGPVGAFEVPPFHVGTMAIAKVAAQLTKVSALETIAGG
EECHHHHHHHHHHHCCCCEEEECCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
GDTIAALNQAGAYDDFTYVSTAGGAFLEWLEGKLLPGVEALRGSQDFTL
CCHHHHHHCCCCCCCCCEEECCCHHHHHHHCCCCCCCHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]