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The map label for this gene is 182680225

Identifier: 182680225

GI number: 182680225

Start: 3779351

End: 3780634

Strand: Reverse

Name: 182680225

Synonym: Bind_3323

Alternate gene names: NA

Gene position: 3780634-3779351 (Counterclockwise)

Preceding gene: 182680236

Following gene: 182680224

Centisome position: 90.66

GC content: 58.02

Gene sequence:

>1284_bases
ATGAATCCGTTTTCCTATGGCATGCATGAAATGACCCATCTGGCGCTGACGCCGGCCCGTATCGTCACGGATGCAACGCG
TCAGTGGTTCAAGAATCCGATCAATCCCTTGTCCTATACGCATGTCGGCCGCAATCTCGCCGCTTCGGCGGAGATTTTCG
AACGGCTGACCCGCCATTATGAAAAGCCGGTCTTTGGCTTTGAGGCTACGACCATTGGCGAGGCGCGTGTGCCGATCCGG
GAAAAGATCGTTTGGCAGAAGCCTTTCGGCCAGCTTTTGCGCTTCGAGCGTGATACGAGCACCGTGCCGGATCTGGCGCC
GACCTTGGCCAGGCAAGCTAAACTGCTCGTCGTCGCACCGATGTCGGGTCATCATGCAACATTGCTGCGGGGAACGGTCG
AAGCTTTTCTTCCCTGTTACGATGTCTATATCACCGATTGGGTGAATGCCCGTCTTGTGCCGAAGGAGGTCGCCAGTTTC
GGCCTCGACGATTATATCGATTATCTCATCGACATCTGCCGGGTCCTCAATGCCGCCAAGACGAGAGGCGAGCCCGACGT
GCCCTTGCATACGATCGGCGTCTGCCAGCCCGCGGTGCCCCTGATCGCCGCCGTTGCCCGTATGGAAGCGCAAAATGATC
CTGCCGTGCCCGCCTCCATGACGCTGATGGGCGGACCAATCGACACGCGCCGCAGTCCGACCGCCGTCAATCTTCTTGCG
CGGGAACGCGGCATGCCCTGGTTCCGCGAGAACTGCATTCATGTCGTGCCCTATAGCTATCCGGGCTTCGGGCGCAGCGT
CTATCCGGGCTTTTTGCAGCTCGCGGGCTTCATGGCGATGAATATCGATCGCCATGTCACCGCCCATATCGACATGTTCA
ATCATCTTGTCGAGAACGATGGTGATTCCGCCGAGAAACATCGTGAATTCTACGATGAATATCTCGCGGTGATGGACCTC
ACCGCCGAATTCTATCTCGAGACGGTGGATGCTGTTTTCGTCAAACATGCCTTGCCGCTTGGGCAGATGCGCCATCACGG
CGAAAAGGTCGATCTTAGCGCGATTCGCCATGTCGCCATTCTCGCGGTCGAGGGCGAGCGCGACGATATTTCAGGGCTCG
GCCAGACCGAGGCCGCGCTCGAATTATGCGACAATGTGCCGGATAAGATGAAGGCCTATCACATGCAGGCAAAGGTCGGT
CATTATGGCGTGTTCAATGGTTCGCGCTATCGCCGTGAGGTCGTGCCGCGCATCCGTGATTTCCACGCCGCCAGCGCCGC
TTGA

Upstream 100 bases:

>100_bases
AAAGAACAAAAAAGACAAAAACATAGAAAGCCTCGTCCGCGCTGAGGGCCTGATCACGGGCGTTTCCAAGGCGAAGGGCG
GGGTTATGGGGGAAATAAAG

Downstream 100 bases:

>100_bases
TCTCATTTTTTGGTGGGGGCTTCGCTCCCACCAAAGAAACTATGGTTTCCAAGGTGCCAGCATGCTAGGCATAAGCTAGG
AGCGCCCGCTCCAAGAAAAG

Product: polyhydroxyalkanoate depolymerase, intracellular

Products: NA

Alternate protein names: Intracellular PHB Depolymerase; Polyhydroxyalkanoate Depolymerase; Poly-Beta-Hydroxyalkanoate Depolymerase; PHB Depolymerase; Poly[D(-)-3-Hydroxybutyrate] Depolymerase; Intracellular Poly(3-Hydroxybutyrate) Depolymerase; Intracellular Polyhydroxyalkanoate Depolymerase; PHB De-Polymerase Domain-Containing Protein; Polyhydroxyalkanoate Depolymerase Intracellular Subfamily; Poly(3-Hydroxyalkanoate) Depolymerase; Poly(3-Hydroxybutyrate) Depolymerase; Alpha/Beta Hydrolase Fold Protein; Intracellular PHB Depolymerase Protein; Poly-Beta-Hydroxyalkanoate Depolymerase-Like Protein; Intracellular Poly[D(-)-3-Hydroxybutyrate] Depolymerase; Poly[D(-)-3-Hydroxybutyrate](Phb) Depolymerase; Poly-Beta-Hydroxyalkanoate/Butyrate Depolymerase; Poly-Beta-Hydroxyalkanoate Depolymerase Protein; Poly[D(-)-3-Hydroxyalkanoate] Depolymerase; Polyhydroxybutanoate Depolymerase; Poly-Beta-Hydroxybutyrate Depolymerase

Number of amino acids: Translated: 427; Mature: 427

Protein sequence:

>427_residues
MNPFSYGMHEMTHLALTPARIVTDATRQWFKNPINPLSYTHVGRNLAASAEIFERLTRHYEKPVFGFEATTIGEARVPIR
EKIVWQKPFGQLLRFERDTSTVPDLAPTLARQAKLLVVAPMSGHHATLLRGTVEAFLPCYDVYITDWVNARLVPKEVASF
GLDDYIDYLIDICRVLNAAKTRGEPDVPLHTIGVCQPAVPLIAAVARMEAQNDPAVPASMTLMGGPIDTRRSPTAVNLLA
RERGMPWFRENCIHVVPYSYPGFGRSVYPGFLQLAGFMAMNIDRHVTAHIDMFNHLVENDGDSAEKHREFYDEYLAVMDL
TAEFYLETVDAVFVKHALPLGQMRHHGEKVDLSAIRHVAILAVEGERDDISGLGQTEAALELCDNVPDKMKAYHMQAKVG
HYGVFNGSRYRREVVPRIRDFHAASAA

Sequences:

>Translated_427_residues
MNPFSYGMHEMTHLALTPARIVTDATRQWFKNPINPLSYTHVGRNLAASAEIFERLTRHYEKPVFGFEATTIGEARVPIR
EKIVWQKPFGQLLRFERDTSTVPDLAPTLARQAKLLVVAPMSGHHATLLRGTVEAFLPCYDVYITDWVNARLVPKEVASF
GLDDYIDYLIDICRVLNAAKTRGEPDVPLHTIGVCQPAVPLIAAVARMEAQNDPAVPASMTLMGGPIDTRRSPTAVNLLA
RERGMPWFRENCIHVVPYSYPGFGRSVYPGFLQLAGFMAMNIDRHVTAHIDMFNHLVENDGDSAEKHREFYDEYLAVMDL
TAEFYLETVDAVFVKHALPLGQMRHHGEKVDLSAIRHVAILAVEGERDDISGLGQTEAALELCDNVPDKMKAYHMQAKVG
HYGVFNGSRYRREVVPRIRDFHAASAA
>Mature_427_residues
MNPFSYGMHEMTHLALTPARIVTDATRQWFKNPINPLSYTHVGRNLAASAEIFERLTRHYEKPVFGFEATTIGEARVPIR
EKIVWQKPFGQLLRFERDTSTVPDLAPTLARQAKLLVVAPMSGHHATLLRGTVEAFLPCYDVYITDWVNARLVPKEVASF
GLDDYIDYLIDICRVLNAAKTRGEPDVPLHTIGVCQPAVPLIAAVARMEAQNDPAVPASMTLMGGPIDTRRSPTAVNLLA
RERGMPWFRENCIHVVPYSYPGFGRSVYPGFLQLAGFMAMNIDRHVTAHIDMFNHLVENDGDSAEKHREFYDEYLAVMDL
TAEFYLETVDAVFVKHALPLGQMRHHGEKVDLSAIRHVAILAVEGERDDISGLGQTEAALELCDNVPDKMKAYHMQAKVG
HYGVFNGSRYRREVVPRIRDFHAASAA

Specific function: Unknown

COG id: COG4553

COG function: function code I; Poly-beta-hydroxyalkanoate depolymerase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 47892; Mature: 47892

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPFSYGMHEMTHLALTPARIVTDATRQWFKNPINPLSYTHVGRNLAASAEIFERLTRHY
CCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCHHHHHCCHHHHHHHHHHHHHHHH
EKPVFGFEATTIGEARVPIREKIVWQKPFGQLLRFERDTSTVPDLAPTLARQAKLLVVAP
CCCCCCCCCCCCCCCCCCHHHHHHCCCCHHHHHHHCCCCCCCCHHHHHHHHCCEEEEEEC
MSGHHATLLRGTVEAFLPCYDVYITDWVNARLVPKEVASFGLDDYIDYLIDICRVLNAAK
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEECHHHHHHCCHHHHHHHHHHHHHHHHHHH
TRGEPDVPLHTIGVCQPAVPLIAAVARMEAQNDPAVPASMTLMGGPIDTRRSPTAVNLLA
CCCCCCCCEEECCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCHHHHHHH
RERGMPWFRENCIHVVPYSYPGFGRSVYPGFLQLAGFMAMNIDRHVTAHIDMFNHLVEND
HHCCCCHHHCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCC
GDSAEKHREFYDEYLAVMDLTAEFYLETVDAVFVKHALPLGQMRHHGEKVDLSAIRHVAI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCHHHHCEEEE
LAVEGERDDISGLGQTEAALELCDNVPDKMKAYHMQAKVGHYGVFNGSRYRREVVPRIRD
EEEECCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
FHAASAA
HHHHCCC
>Mature Secondary Structure
MNPFSYGMHEMTHLALTPARIVTDATRQWFKNPINPLSYTHVGRNLAASAEIFERLTRHY
CCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCHHHHHCCHHHHHHHHHHHHHHHH
EKPVFGFEATTIGEARVPIREKIVWQKPFGQLLRFERDTSTVPDLAPTLARQAKLLVVAP
CCCCCCCCCCCCCCCCCCHHHHHHCCCCHHHHHHHCCCCCCCCHHHHHHHHCCEEEEEEC
MSGHHATLLRGTVEAFLPCYDVYITDWVNARLVPKEVASFGLDDYIDYLIDICRVLNAAK
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEECHHHHHHCCHHHHHHHHHHHHHHHHHHH
TRGEPDVPLHTIGVCQPAVPLIAAVARMEAQNDPAVPASMTLMGGPIDTRRSPTAVNLLA
CCCCCCCCEEECCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCHHHHHHH
RERGMPWFRENCIHVVPYSYPGFGRSVYPGFLQLAGFMAMNIDRHVTAHIDMFNHLVEND
HHCCCCHHHCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCC
GDSAEKHREFYDEYLAVMDLTAEFYLETVDAVFVKHALPLGQMRHHGEKVDLSAIRHVAI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCHHHHCEEEE
LAVEGERDDISGLGQTEAALELCDNVPDKMKAYHMQAKVGHYGVFNGSRYRREVVPRIRD
EEEECCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
FHAASAA
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA