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The map label for this gene is kefA [H]

Identifier: 182680125

GI number: 182680125

Start: 3664915

End: 3667515

Strand: Reverse

Name: kefA [H]

Synonym: Bind_3222

Alternate gene names: 182680125

Gene position: 3667515-3664915 (Counterclockwise)

Preceding gene: 182680126

Following gene: 182680124

Centisome position: 87.95

GC content: 59.21

Gene sequence:

>2601_bases
GTGGTGAAATTTCTTGCTTTTCTGTTGTGTCTATTTCTCGTCGCATCCTTGCCTATTGAGCATGGTGTTGCGGCACAGAC
GGCGCCAGCAGCACCCGCCGCGCCTGCCGGACCCGCAGCGCCCGACAGCGCTTCTGCCCCGACGCCCCTGGCGGTCGCGA
CGCCGGCGCTTCCCCCGCAATGGGGGCGCGATCTCGATCAAGTGCATGCCGGGCTCGACCAGATTGATCAGAGCCTGCAG
CGCCATGACTTGCGGGCCGACGATTTCGATACGCTTCAGGCCCAGATTCCGCCCTTACAGACCAAGCTCAATGATGTGAT
CGGCCGTCTCACGCCACGGCTGGCGGGTGTCAAGCAGCGGCTCGATCAATTGGGGCCAAAGCCTGCCGATGACGCGCCCC
CGGAAAGCCCGGCCGTGACGGCTGATCGGGCCGATCAGCAAAAAAACTATACGGATACGGATGAGCTGCTGAAACGGGCC
AAGCTGCTCGCGGTTCAGGCCGACCAGGCTAATAATTTCATCATTTCACAACGGCGTGGCCTGATCACGCGATCCCTTTT
CGCGAGGGTTAAGAGCCCGCTCGCGCCTTCAGTCTGGATGGAAATGATCCAAGAGGTCCCGCGCGAAATCGGCGCCCTGG
AGCTCGTTTTCAAAGACGGCTTCGCGCAGATGAATGCGGTGCTGATCGGCTGGCGCATGCCGGCCTTTTGGGGCTCGCTC
GCGGGGCTTGCTCTTCTTTATTGGCCGGCTTGGCGCCTTTCCTTCAAAATTCTCTCCCGCGAATCGAAGATCACCACGCC
GAATAATTTGCAGAAGGCTGCCGCCGCCTGGTGGAGCGCGCTTGTCGTCATGATTCTGCCGGGTCTGGTGGTGTTCGGAG
TCGGTCAGATTTTCGCCGGCTTCGGAATCAACAGCACGCGCCTGCAACCTTTTTTCACGGCCTTGGGACTTGGGGCCCTG
CATCTGACCTCGGTCGCGGCAGTGGGCAATGGATTCCTCGCCCTGCGGCGCCCGAGCTGGCGTCTGATCGAGCTCAATGA
CGCCCGCGCGCAACGCATTTTAACCGCGGCCCTGATCCTTGCCTTTGTCGTCGCGAGCATCAAGGTCGTTGAGGCTTTGA
ACGAAATCATCGTCGCCTCGCTGCCTTTCGCTATTCTGACGCGTGGATTTGGCGCGCTGTTCGGCGCCCTGGTGATTTTC
GTATCCCTGTGGGGCGCTACCTCTCTGGAGGCGGATGAAGACGGTGACGCGCCGGCGGGGAACCAGGCGAAGGATCCGCA
AGCCAATACGGATTATATCGGCATTGCCCGTATCCTCCTCTGGGGCGTCGCCATCGTGGTCGTGGCGGCGACGCTCAGTG
GTTTCATCGCCTTTGCGAATTTCCTGCTGCAGCAGGTTTTATGGGTCACCGGCGTCGTCTGTATTTTCATCATGGCCATG
ATGCTGTCCGACAGCGTCATTGTCACTTCGATCAATCCGACCTCACGGTTCGGCCATCGCCTCATCACCAAAGTTGGCCT
GCATCGGCAGACGATCGAAATCGGTGCGATCCTCTTGTCCGGCGCGATGCGGGTCCTCTTGCTCTTCGTTGCGATCTTCT
TCCTGCTCGCGCCTTGGGGGCTGCAGTCCAACGATGTCTCGAACGATCTCGGGGCGGCGTTCTTCGGCTTCAAAGTCGGT
GGGGTGACGATCTCGCTGTCCAGCATCATCATGGGCATTGCGGTCTTCACCGCGACATTTCTCGCGGTGAAGGCCTTTCA
GAAATGGCTCGACAAGCAACTCCTGCCCAAAACGCGGCTTGATGCTGGCTTACGCAATTCCATCGGCACCAGTCTCGGTT
ATTTGGCGATCATCCTGGCGGTGGCGTCCTCTTCCGCCTATCTCGGCCTCAATTTTGAGAGGCTTGCACTTCTCGCCGGC
GCGCTCTCCGTCGGTATCGGTTTCGGCCTGCAATCGATCATCAATAATTTCGTGTCCGGCCTCATTCTTCTCTGGGAAAG
AGCGGTTCGCGTCGGTGACTGGATCGTCGTTGGCGGCGAGCAGGGCTTCGTGCGCAAGATCAACGTGCGTTCGACGGAGA
TCGAGACCTTCGATCGCGCCGCCGTCATCATTCCCAATTCCAATATCATTAGCGGCGTCGTCAAAAATCTCGTCCGCAAC
GATACGACTTCGCGCGTCCTGATCCCGATCACCGTTTCAGCCACCGCCAATCCAGAACAGGTGAGAAGCGTGTTGCTCGA
AATCGCCAAGGCCAATGAAATGGTGCTCAGCCTGCCGAGCCCTCAGGTCTTCTTCATTGAGGTCACGGCGTCCAGCCTGA
CCTTTGAGCTGAATTGCTATGTCGGTGACGTCATCACCTTGCGGCGGGTGAAAAGCGATCTCTATTTCGAGATTTATCGT
CGCTTCAAGGAAGAGAAATTCTTCAGTGGCGCCCCCGCCGACCCGACCGTGGTCGATATCAAGGGGCTGGGCCCCTTAAG
CGCTATGCTCTCTCGCGCCGATGGTCCCTTGTCCGCCGATGCGGAAACAGCCGATAGCCCCTCGATCACCGATTTGCAGA
AAGCCGAAGTGCTGCTTCAACGCGCTGGCAACGGGCGCTGA

Upstream 100 bases:

>100_bases
GAAACCAGACTGGCGACATGTGCCGAACCTTCCTCTTCATTTCATGCAGCATCGGATTTTTTTGAAAGCCGATGATCGAT
CGTCTTCTAGGAAATCGCGC

Downstream 100 bases:

>100_bases
ACTCCCGCGCATGGACGGAATTCGCTTCCCCGCGGCAGGGCGCATCGGCCTTCCGACAATCATGGCCCTATCAGCGCTCG
CTGGCTGTGCCGTCGCCCCG

Product: MscS mechanosensitive ion channel

Products: NA

Alternate protein names: Protein AefA [H]

Number of amino acids: Translated: 866; Mature: 866

Protein sequence:

>866_residues
MVKFLAFLLCLFLVASLPIEHGVAAQTAPAAPAAPAGPAAPDSASAPTPLAVATPALPPQWGRDLDQVHAGLDQIDQSLQ
RHDLRADDFDTLQAQIPPLQTKLNDVIGRLTPRLAGVKQRLDQLGPKPADDAPPESPAVTADRADQQKNYTDTDELLKRA
KLLAVQADQANNFIISQRRGLITRSLFARVKSPLAPSVWMEMIQEVPREIGALELVFKDGFAQMNAVLIGWRMPAFWGSL
AGLALLYWPAWRLSFKILSRESKITTPNNLQKAAAAWWSALVVMILPGLVVFGVGQIFAGFGINSTRLQPFFTALGLGAL
HLTSVAAVGNGFLALRRPSWRLIELNDARAQRILTAALILAFVVASIKVVEALNEIIVASLPFAILTRGFGALFGALVIF
VSLWGATSLEADEDGDAPAGNQAKDPQANTDYIGIARILLWGVAIVVVAATLSGFIAFANFLLQQVLWVTGVVCIFIMAM
MLSDSVIVTSINPTSRFGHRLITKVGLHRQTIEIGAILLSGAMRVLLLFVAIFFLLAPWGLQSNDVSNDLGAAFFGFKVG
GVTISLSSIIMGIAVFTATFLAVKAFQKWLDKQLLPKTRLDAGLRNSIGTSLGYLAIILAVASSSAYLGLNFERLALLAG
ALSVGIGFGLQSIINNFVSGLILLWERAVRVGDWIVVGGEQGFVRKINVRSTEIETFDRAAVIIPNSNIISGVVKNLVRN
DTTSRVLIPITVSATANPEQVRSVLLEIAKANEMVLSLPSPQVFFIEVTASSLTFELNCYVGDVITLRRVKSDLYFEIYR
RFKEEKFFSGAPADPTVVDIKGLGPLSAMLSRADGPLSADAETADSPSITDLQKAEVLLQRAGNGR

Sequences:

>Translated_866_residues
MVKFLAFLLCLFLVASLPIEHGVAAQTAPAAPAAPAGPAAPDSASAPTPLAVATPALPPQWGRDLDQVHAGLDQIDQSLQ
RHDLRADDFDTLQAQIPPLQTKLNDVIGRLTPRLAGVKQRLDQLGPKPADDAPPESPAVTADRADQQKNYTDTDELLKRA
KLLAVQADQANNFIISQRRGLITRSLFARVKSPLAPSVWMEMIQEVPREIGALELVFKDGFAQMNAVLIGWRMPAFWGSL
AGLALLYWPAWRLSFKILSRESKITTPNNLQKAAAAWWSALVVMILPGLVVFGVGQIFAGFGINSTRLQPFFTALGLGAL
HLTSVAAVGNGFLALRRPSWRLIELNDARAQRILTAALILAFVVASIKVVEALNEIIVASLPFAILTRGFGALFGALVIF
VSLWGATSLEADEDGDAPAGNQAKDPQANTDYIGIARILLWGVAIVVVAATLSGFIAFANFLLQQVLWVTGVVCIFIMAM
MLSDSVIVTSINPTSRFGHRLITKVGLHRQTIEIGAILLSGAMRVLLLFVAIFFLLAPWGLQSNDVSNDLGAAFFGFKVG
GVTISLSSIIMGIAVFTATFLAVKAFQKWLDKQLLPKTRLDAGLRNSIGTSLGYLAIILAVASSSAYLGLNFERLALLAG
ALSVGIGFGLQSIINNFVSGLILLWERAVRVGDWIVVGGEQGFVRKINVRSTEIETFDRAAVIIPNSNIISGVVKNLVRN
DTTSRVLIPITVSATANPEQVRSVLLEIAKANEMVLSLPSPQVFFIEVTASSLTFELNCYVGDVITLRRVKSDLYFEIYR
RFKEEKFFSGAPADPTVVDIKGLGPLSAMLSRADGPLSADAETADSPSITDLQKAEVLLQRAGNGR
>Mature_866_residues
MVKFLAFLLCLFLVASLPIEHGVAAQTAPAAPAAPAGPAAPDSASAPTPLAVATPALPPQWGRDLDQVHAGLDQIDQSLQ
RHDLRADDFDTLQAQIPPLQTKLNDVIGRLTPRLAGVKQRLDQLGPKPADDAPPESPAVTADRADQQKNYTDTDELLKRA
KLLAVQADQANNFIISQRRGLITRSLFARVKSPLAPSVWMEMIQEVPREIGALELVFKDGFAQMNAVLIGWRMPAFWGSL
AGLALLYWPAWRLSFKILSRESKITTPNNLQKAAAAWWSALVVMILPGLVVFGVGQIFAGFGINSTRLQPFFTALGLGAL
HLTSVAAVGNGFLALRRPSWRLIELNDARAQRILTAALILAFVVASIKVVEALNEIIVASLPFAILTRGFGALFGALVIF
VSLWGATSLEADEDGDAPAGNQAKDPQANTDYIGIARILLWGVAIVVVAATLSGFIAFANFLLQQVLWVTGVVCIFIMAM
MLSDSVIVTSINPTSRFGHRLITKVGLHRQTIEIGAILLSGAMRVLLLFVAIFFLLAPWGLQSNDVSNDLGAAFFGFKVG
GVTISLSSIIMGIAVFTATFLAVKAFQKWLDKQLLPKTRLDAGLRNSIGTSLGYLAIILAVASSSAYLGLNFERLALLAG
ALSVGIGFGLQSIINNFVSGLILLWERAVRVGDWIVVGGEQGFVRKINVRSTEIETFDRAAVIIPNSNIISGVVKNLVRN
DTTSRVLIPITVSATANPEQVRSVLLEIAKANEMVLSLPSPQVFFIEVTASSLTFELNCYVGDVITLRRVKSDLYFEIYR
RFKEEKFFSGAPADPTVVDIKGLGPLSAMLSRADGPLSADAETADSPSITDLQKAEVLLQRAGNGR

Specific function: Mechanosensitive channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell, although it does not appear to have a major role in osmolarity regulation. Form

COG id: COG3264

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI1786670, Length=242, Percent_Identity=35.9504132231405, Blast_Score=147, Evalue=3e-36,
Organism=Escherichia coli, GI2367355, Length=221, Percent_Identity=33.9366515837104, Blast_Score=134, Evalue=3e-32,
Organism=Escherichia coli, GI1789291, Length=240, Percent_Identity=22.5, Blast_Score=73, Evalue=9e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685
- InterPro:   IPR006686
- InterPro:   IPR011014 [H]

Pfam domain/function: PF00924 MS_channel [H]

EC number: NA

Molecular weight: Translated: 93210; Mature: 93210

Theoretical pI: Translated: 9.00; Mature: 9.00

Prosite motif: PS01246 UPF0003

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKFLAFLLCLFLVASLPIEHGVAAQTAPAAPAAPAGPAAPDSASAPTPLAVATPALPPQ
CHHHHHHHHHHHHHHHCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCC
WGRDLDQVHAGLDQIDQSLQRHDLRADDFDTLQAQIPPLQTKLNDVIGRLTPRLAGVKQR
CCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
LDQLGPKPADDAPPESPAVTADRADQQKNYTDTDELLKRAKLLAVQADQANNFIISQRRG
HHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHEEECCCCCCEEEHHHCC
LITRSLFARVKSPLAPSVWMEMIQEVPREIGALELVFKDGFAQMNAVLIGWRMPAFWGSL
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCEEEEHHHCHHHHHHEEEECCCHHHHHHH
AGLALLYWPAWRLSFKILSRESKITTPNNLQKAAAAWWSALVVMILPGLVVFGVGQIFAG
HHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
FGINSTRLQPFFTALGLGALHLTSVAAVGNGFLALRRPSWRLIELNDARAQRILTAALIL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCEEEEEECHHHHHHHHHHHHHH
AFVVASIKVVEALNEIIVASLPFAILTRGFGALFGALVIFVSLWGATSLEADEDGDAPAG
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
NQAKDPQANTDYIGIARILLWGVAIVVVAATLSGFIAFANFLLQQVLWVTGVVCIFIMAM
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MLSDSVIVTSINPTSRFGHRLITKVGLHRQTIEIGAILLSGAMRVLLLFVAIFFLLAPWG
HHCCCEEEEECCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
LQSNDVSNDLGAAFFGFKVGGVTISLSSIIMGIAVFTATFLAVKAFQKWLDKQLLPKTRL
CCCCCCCHHHHHHHHHEEECCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
DAGLRNSIGTSLGYLAIILAVASSSAYLGLNFERLALLAGALSVGIGFGLQSIINNFVSG
HHHHHHHHHHHHHHHHHHHHHHCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LILLWERAVRVGDWIVVGGEQGFVRKINVRSTEIETFDRAAVIIPNSNIISGVVKNLVRN
HHHHHHHHHHCCCEEEECCCCCCEEEEECCCCHHHHHCCEEEEECCCHHHHHHHHHHHCC
DTTSRVLIPITVSATANPEQVRSVLLEIAKANEMVLSLPSPQVFFIEVTASSLTFELNCY
CCCCEEEEEEEEECCCCHHHHHHHHHHHHHCCCEEEECCCCCEEEEEEEECCEEEEEEEE
VGDVITLRRVKSDLYFEIYRRFKEEKFFSGAPADPTVVDIKGLGPLSAMLSRADGPLSAD
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHHCCCCCCCCC
AETADSPSITDLQKAEVLLQRAGNGR
CCCCCCCCCCHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MVKFLAFLLCLFLVASLPIEHGVAAQTAPAAPAAPAGPAAPDSASAPTPLAVATPALPPQ
CHHHHHHHHHHHHHHHCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCC
WGRDLDQVHAGLDQIDQSLQRHDLRADDFDTLQAQIPPLQTKLNDVIGRLTPRLAGVKQR
CCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
LDQLGPKPADDAPPESPAVTADRADQQKNYTDTDELLKRAKLLAVQADQANNFIISQRRG
HHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHEEECCCCCCEEEHHHCC
LITRSLFARVKSPLAPSVWMEMIQEVPREIGALELVFKDGFAQMNAVLIGWRMPAFWGSL
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCEEEEHHHCHHHHHHEEEECCCHHHHHHH
AGLALLYWPAWRLSFKILSRESKITTPNNLQKAAAAWWSALVVMILPGLVVFGVGQIFAG
HHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
FGINSTRLQPFFTALGLGALHLTSVAAVGNGFLALRRPSWRLIELNDARAQRILTAALIL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCEEEEEECHHHHHHHHHHHHHH
AFVVASIKVVEALNEIIVASLPFAILTRGFGALFGALVIFVSLWGATSLEADEDGDAPAG
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
NQAKDPQANTDYIGIARILLWGVAIVVVAATLSGFIAFANFLLQQVLWVTGVVCIFIMAM
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MLSDSVIVTSINPTSRFGHRLITKVGLHRQTIEIGAILLSGAMRVLLLFVAIFFLLAPWG
HHCCCEEEEECCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
LQSNDVSNDLGAAFFGFKVGGVTISLSSIIMGIAVFTATFLAVKAFQKWLDKQLLPKTRL
CCCCCCCHHHHHHHHHEEECCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
DAGLRNSIGTSLGYLAIILAVASSSAYLGLNFERLALLAGALSVGIGFGLQSIINNFVSG
HHHHHHHHHHHHHHHHHHHHHHCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LILLWERAVRVGDWIVVGGEQGFVRKINVRSTEIETFDRAAVIIPNSNIISGVVKNLVRN
HHHHHHHHHHCCCEEEECCCCCCEEEEECCCCHHHHHCCEEEEECCCHHHHHHHHHHHCC
DTTSRVLIPITVSATANPEQVRSVLLEIAKANEMVLSLPSPQVFFIEVTASSLTFELNCY
CCCCEEEEEEEEECCCCHHHHHHHHHHHHHCCCEEEECCCCCEEEEEEEECCEEEEEEEE
VGDVITLRRVKSDLYFEIYRRFKEEKFFSGAPADPTVVDIKGLGPLSAMLSRADGPLSAD
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHHCCCCCCCCC
AETADSPSITDLQKAEVLLQRAGNGR
CCCCCCCCCCHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9278503; 10202137 [H]