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The map label for this gene is xylF [H]

Identifier: 182679896

GI number: 182679896

Start: 3396603

End: 3397622

Strand: Reverse

Name: xylF [H]

Synonym: Bind_2989

Alternate gene names: 182679896

Gene position: 3397622-3396603 (Counterclockwise)

Preceding gene: 182679897

Following gene: 182679895

Centisome position: 81.47

GC content: 52.84

Gene sequence:

>1020_bases
ATGTTCCTTCGCCTCTCCAAGACCCGATCCTTTGGGATAGCAGGGCTCATCGGCGCGGCAATCTTAAGTACGCTTGCCAT
CGCCAACTACTTCATAGCGCGCCGCACGGAGCGTCGACACCCATCAAAGGGTTCCTTCCTGACCGTAGAGGGGGTTAGGC
TGCATTACAGCGATCGGGGTGAAGGTGATCCGGCCGTGCTCATTCACGGTAACGCCGTAACAGGTGATGATTGGAACACC
AGTGGTGTAGCTGATCGTTTGTTACAAACCCACAGAGTTATCATCTTTGACCGCCCTGGTTTCGGGCATAGCGATCGCCC
TCGTGGGCATATTTGGACACCCGAAAAACAAGCCGAATTGCTCCATAAAGCTATGCGTAAGCTCAACATTGAGCGCCCGA
TCATCGTGGCCCACTCTTCGGGCACGCTTGTGGCGCTCGCGCTCGCGGCGCGCCATAAGGCCGATCTAGGCGGCCTTGTG
CTTGTGTCTGGTTACTATTTTTGGACTCTGCGGCTAGAGGTCGTATTGGCCGCGGTAGGAGCGCTCCCTCTGCTAGGCGA
CATACTGAATTATACCATTTCTCCCTTGCTTGGTTGGCTCGAGATGCCCCTACTCAAATGGCTGATGTTTTCTCCTACTC
AGATCCCTAAAAGATTCCAGGCTGAATATTCGACTGCGATGGCCTTGCGACCGTCGCAGATTCGGGCCACCTCTGAAGAT
GATGTGCTCATGATTCCTGCGGCTATAGCCTTGCGTAGGTCCTATAAGGATATGACTTTACCTGTCGCTCTCATTGCAGG
AGACAGCGATAAAATTGTCTTTAAACAAGCGAGCGAACACCTTGCAGCCTGCCTCCCAAACAGTGTGCTGCAAATCGTAA
AAGGGGCGGGTCATATGGTTCATTATACAGCCCCGCTACTGATAACAGAGGCGGTTGATAACATCGCAAAGGATTTGTCC
GCAATAGGACACGGCAAGAATGAGGGAATTTCCGAACCCGCGCGGTTGTTCCAAAAATAA

Upstream 100 bases:

>100_bases
TGGCGTTGATGTGATTCAGTTGTGGCGAATGAAGGGGGCGCCATGATTTTTCACTTCCACGGCCTCCAATATTCTGCGGC
ATACATAAAGGGCTTTTATT

Downstream 100 bases:

>100_bases
TAGGTTAACGGCCTATTGAAGAAAAGCATATGCAGCCGCTAAAGACCACAGCCTGGCAGATTGAGTCACACCTACATTTC
AGGATTGGTTGGATTCGCAG

Product: alpha/beta hydrolase fold protein

Products: 2-oxopent-4-enoate; succinate [C]

Alternate protein names: HMSH [H]

Number of amino acids: Translated: 339; Mature: 339

Protein sequence:

>339_residues
MFLRLSKTRSFGIAGLIGAAILSTLAIANYFIARRTERRHPSKGSFLTVEGVRLHYSDRGEGDPAVLIHGNAVTGDDWNT
SGVADRLLQTHRVIIFDRPGFGHSDRPRGHIWTPEKQAELLHKAMRKLNIERPIIVAHSSGTLVALALAARHKADLGGLV
LVSGYYFWTLRLEVVLAAVGALPLLGDILNYTISPLLGWLEMPLLKWLMFSPTQIPKRFQAEYSTAMALRPSQIRATSED
DVLMIPAAIALRRSYKDMTLPVALIAGDSDKIVFKQASEHLAACLPNSVLQIVKGAGHMVHYTAPLLITEAVDNIAKDLS
AIGHGKNEGISEPARLFQK

Sequences:

>Translated_339_residues
MFLRLSKTRSFGIAGLIGAAILSTLAIANYFIARRTERRHPSKGSFLTVEGVRLHYSDRGEGDPAVLIHGNAVTGDDWNT
SGVADRLLQTHRVIIFDRPGFGHSDRPRGHIWTPEKQAELLHKAMRKLNIERPIIVAHSSGTLVALALAARHKADLGGLV
LVSGYYFWTLRLEVVLAAVGALPLLGDILNYTISPLLGWLEMPLLKWLMFSPTQIPKRFQAEYSTAMALRPSQIRATSED
DVLMIPAAIALRRSYKDMTLPVALIAGDSDKIVFKQASEHLAACLPNSVLQIVKGAGHMVHYTAPLLITEAVDNIAKDLS
AIGHGKNEGISEPARLFQK
>Mature_339_residues
MFLRLSKTRSFGIAGLIGAAILSTLAIANYFIARRTERRHPSKGSFLTVEGVRLHYSDRGEGDPAVLIHGNAVTGDDWNT
SGVADRLLQTHRVIIFDRPGFGHSDRPRGHIWTPEKQAELLHKAMRKLNIERPIIVAHSSGTLVALALAARHKADLGGLV
LVSGYYFWTLRLEVVLAAVGALPLLGDILNYTISPLLGWLEMPLLKWLMFSPTQIPKRFQAEYSTAMALRPSQIRATSED
DVLMIPAAIALRRSYKDMTLPVALIAGDSDKIVFKQASEHLAACLPNSVLQIVKGAGHMVHYTAPLLITEAVDNIAKDLS
AIGHGKNEGISEPARLFQK

Specific function: Conversion of 2-hydroxymuconic acid semialdehyde to 2- hydroxypent-2,4-dienoate [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dmpD/todF/xylF esterase family [H]

Homologues:

Organism=Homo sapiens, GI29294637, Length=290, Percent_Identity=26.2068965517241, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI29294635, Length=290, Percent_Identity=26.2068965517241, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI29294639, Length=290, Percent_Identity=26.2068965517241, Blast_Score=69, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: 3.7.1.- [C]

Molecular weight: Translated: 37172; Mature: 37172

Theoretical pI: Translated: 10.04; Mature: 10.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFLRLSKTRSFGIAGLIGAAILSTLAIANYFIARRTERRHPSKGSFLTVEGVRLHYSDRG
CEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEEECCCC
EGDPAVLIHGNAVTGDDWNTSGVADRLLQTHRVIIFDRPGFGHSDRPRGHIWTPEKQAEL
CCCCEEEEECCEECCCCCCCHHHHHHHHHHCEEEEEECCCCCCCCCCCCCCCCCHHHHHH
LHKAMRKLNIERPIIVAHSSGTLVALALAARHKADLGGLVLVSGYYFWTLRLEVVLAAVG
HHHHHHHCCCCCCEEEEECCCCEEEHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHHHH
ALPLLGDILNYTISPLLGWLEMPLLKWLMFSPTQIPKRFQAEYSTAMALRPSQIRATSED
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHEECCHHCCCCCCC
DVLMIPAAIALRRSYKDMTLPVALIAGDSDKIVFKQASEHLAACLPNSVLQIVKGAGHMV
CEEEEHHHHHHHHHCCCCEEEEEEEECCCCCEEHHHHHHHHHHHCCHHHHHHHHCCCCEE
HYTAPLLITEAVDNIAKDLSAIGHGKNEGISEPARLFQK
EEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCC
>Mature Secondary Structure
MFLRLSKTRSFGIAGLIGAAILSTLAIANYFIARRTERRHPSKGSFLTVEGVRLHYSDRG
CEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEEECCCC
EGDPAVLIHGNAVTGDDWNTSGVADRLLQTHRVIIFDRPGFGHSDRPRGHIWTPEKQAEL
CCCCEEEEECCEECCCCCCCHHHHHHHHHHCEEEEEECCCCCCCCCCCCCCCCCHHHHHH
LHKAMRKLNIERPIIVAHSSGTLVALALAARHKADLGGLVLVSGYYFWTLRLEVVLAAVG
HHHHHHHCCCCCCEEEEECCCCEEEHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHHHH
ALPLLGDILNYTISPLLGWLEMPLLKWLMFSPTQIPKRFQAEYSTAMALRPSQIRATSED
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHEECCHHCCCCCCC
DVLMIPAAIALRRSYKDMTLPVALIAGDSDKIVFKQASEHLAACLPNSVLQIVKGAGHMV
CEEEEHHHHHHHHHCCCCEEEEEEEECCCCCEEHHHHHHHHHHHCCHHHHHHHHCCCCEE
HYTAPLLITEAVDNIAKDLSAIGHGKNEGISEPARLFQK
EEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-hydroxy-6-ketononadienedicarboxylate; H2O [C]

Specific reaction: 2-hydroxy-6-ketononadienedicarboxylate + H2O = 2-oxopent-4-enoate + succinate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1791759 [H]