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The map label for this gene is pks2 [H]

Identifier: 182679871

GI number: 182679871

Start: 3368422

End: 3369417

Strand: Reverse

Name: pks2 [H]

Synonym: Bind_2961

Alternate gene names: 182679871

Gene position: 3369417-3368422 (Counterclockwise)

Preceding gene: 182679873

Following gene: 182679870

Centisome position: 80.8

GC content: 59.74

Gene sequence:

>996_bases
GTGATCCAATCGCTTCCCGAGAAGCATCAGGTATGGGCTTGGCCTGATAAAGGTGAACCGCAGAGCCTGCGGTTGGAGAT
ACGCACCCGCCCAATTCCGACTCCCGGTGAGGTTCTGGTTGCCAATAGTGCCATTGGCCTCAATCCAGTCGATTGGAAAA
TGATCGAATGGGGTCATGCGGCATGGTCGGCCGGCCATGTCCCTGGTGTCGATGGGGTCGGCGTCATCGCCGCGACGGGT
GATGGCGTACGCCTGCCCATCGGTTTGAGGGGCGCTTATCACCAGTCGCTTGCACGCGATGGCAGCTTCGCAGAGTTCAC
CATAATCGACGCCAGGAGCATCCTTCCGATTCCGGCCAGCGTCGCAGACGTGGTTGCGGCGGCATTGCCATGCCCCGGTT
TGACGGCTTGGCAAGCCCTCCGGAAAGTGCCTCGGCGGATGGTGCGGGATGTTCTCGTAACCGGCGCTGGCGGATCGGTC
GGCTCCATCCTCGCGCAACTCGCCGCGCGCTGCGGCTGGCGCGTTTGGGTGACGGCTGCTTCAGCCCATCGGGATAAGCT
TCTGGCGCTCGGCATCACGGGAGTCTTCGACTATCGCGATCCAAACTGGTGCGACAGACTCGTGGAGAGTTTGGGGCCAC
GTCGCCTTTACGCGATCTTCGACACCGTAAGCGGCGAGCACGCCCAGACGCTCGCCCCGATGCTCGGCTATAATGGACAT
CTGATCTGCATTCAGGATCGGATCGAGGAAAATCCGCTGCCGGCCTTTACCACCGCCGTTTCGCTGCATGAAGTGGCTCT
CAACAGCGTCCATCTAAACGCCACGGACGAAGACTGGCAAGAATGGCGCTTTGCTGGTGCGGAGTTGTTCCAGATGCTTG
AAACTGGCGCACTTCAATTGCCGCCTATCGAAGTTCGCTCCTTCGATGCACTTCCGCTTACCCTTACCGACTTGAAAAGC
AGAGCGCATAAGGGGAAACTTGTCGTGGTTACGTGA

Upstream 100 bases:

>100_bases
TGTAAACCTGATGTCTGATTTCAGAGATATGATCCTACCATCATATCTTAGTGGACGCTATCTCTTGAAAGTGAAAGTTC
GCCATTCTCAAGAGGTTTTC

Downstream 100 bases:

>100_bases
TATAAGCGGTGTTCACCAACTCCGCCTGCCCGCCAGTGACGATGGCATGGGCATACAAAACGAAGTTGCCGACACCATAC
GTCGAACAAGCAAGAGCTTC

Product: alcohol dehydrogenase

Products: NADP(+); Semiquinone. [C]

Alternate protein names: Polyketide synthase pks2 [H]

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MIQSLPEKHQVWAWPDKGEPQSLRLEIRTRPIPTPGEVLVANSAIGLNPVDWKMIEWGHAAWSAGHVPGVDGVGVIAATG
DGVRLPIGLRGAYHQSLARDGSFAEFTIIDARSILPIPASVADVVAAALPCPGLTAWQALRKVPRRMVRDVLVTGAGGSV
GSILAQLAARCGWRVWVTAASAHRDKLLALGITGVFDYRDPNWCDRLVESLGPRRLYAIFDTVSGEHAQTLAPMLGYNGH
LICIQDRIEENPLPAFTTAVSLHEVALNSVHLNATDEDWQEWRFAGAELFQMLETGALQLPPIEVRSFDALPLTLTDLKS
RAHKGKLVVVT

Sequences:

>Translated_331_residues
MIQSLPEKHQVWAWPDKGEPQSLRLEIRTRPIPTPGEVLVANSAIGLNPVDWKMIEWGHAAWSAGHVPGVDGVGVIAATG
DGVRLPIGLRGAYHQSLARDGSFAEFTIIDARSILPIPASVADVVAAALPCPGLTAWQALRKVPRRMVRDVLVTGAGGSV
GSILAQLAARCGWRVWVTAASAHRDKLLALGITGVFDYRDPNWCDRLVESLGPRRLYAIFDTVSGEHAQTLAPMLGYNGH
LICIQDRIEENPLPAFTTAVSLHEVALNSVHLNATDEDWQEWRFAGAELFQMLETGALQLPPIEVRSFDALPLTLTDLKS
RAHKGKLVVVT
>Mature_331_residues
MIQSLPEKHQVWAWPDKGEPQSLRLEIRTRPIPTPGEVLVANSAIGLNPVDWKMIEWGHAAWSAGHVPGVDGVGVIAATG
DGVRLPIGLRGAYHQSLARDGSFAEFTIIDARSILPIPASVADVVAAALPCPGLTAWQALRKVPRRMVRDVLVTGAGGSV
GSILAQLAARCGWRVWVTAASAHRDKLLALGITGVFDYRDPNWCDRLVESLGPRRLYAIFDTVSGEHAQTLAPMLGYNGH
LICIQDRIEENPLPAFTTAVSLHEVALNSVHLNATDEDWQEWRFAGAELFQMLETGALQLPPIEVRSFDALPLTLTDLKS
RAHKGKLVVVT

Specific function: Catalyzes the synthesis of methyl phthioceranic acids and/or hydroxyphthioceranic acids that are the major acyl constituents of cell wall lipids [H]

COG id: COG0604

COG function: function code CR; NADPH:quinone reductase and related Zn-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 acyl carrier domain [H]

Homologues:

Organism=Homo sapiens, GI194239674, Length=273, Percent_Identity=27.8388278388278, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI13236495, Length=273, Percent_Identity=27.8388278388278, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI194239676, Length=196, Percent_Identity=30.6122448979592, Blast_Score=67, Evalue=3e-11,
Organism=Escherichia coli, GI1790485, Length=209, Percent_Identity=29.6650717703349, Blast_Score=67, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17507255, Length=243, Percent_Identity=30.0411522633745, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001227
- InterPro:   IPR009081
- InterPro:   IPR014043
- InterPro:   IPR016035
- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR000794
- InterPro:   IPR002198
- InterPro:   IPR011032
- InterPro:   IPR018201
- InterPro:   IPR014031
- InterPro:   IPR014030
- InterPro:   IPR016036
- InterPro:   IPR016040
- InterPro:   IPR006163
- InterPro:   IPR020842
- InterPro:   IPR020801
- InterPro:   IPR020807
- InterPro:   IPR020843
- InterPro:   IPR006162
- InterPro:   IPR016039
- InterPro:   IPR016038 [H]

Pfam domain/function: PF00698 Acyl_transf_1; PF08240 ADH_N; PF00106 adh_short; PF00107 ADH_zinc_N; PF00109 ketoacyl-synt; PF02801 Ketoacyl-synt_C; PF00550 PP-binding [H]

EC number: 1.6.5.5 [C]

Molecular weight: Translated: 35948; Mature: 35948

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQSLPEKHQVWAWPDKGEPQSLRLEIRTRPIPTPGEVLVANSAIGLNPVDWKMIEWGHA
CCCCCCCCCCEEECCCCCCCCEEEEEEEECCCCCCCCEEEECCCCCCCCCCEEEEECCCH
AWSAGHVPGVDGVGVIAATGDGVRLPIGLRGAYHQSLARDGSFAEFTIIDARSILPIPAS
HCCCCCCCCCCCCEEEEECCCCEEEEEECCHHHHHHHHCCCCCEEEEEEECCCCCCCCHH
VADVVAAALPCPGLTAWQALRKVPRRMVRDVLVTGAGGSVGSILAQLAARCGWRVWVTAA
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHCCCEEEEEEC
SAHRDKLLALGITGVFDYRDPNWCDRLVESLGPRRLYAIFDTVSGEHAQTLAPMLGYNGH
CCCCCEEEEEEEEEEECCCCCHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHCCCCC
LICIQDRIEENPLPAFTTAVSLHEVALNSVHLNATDEDWQEWRFAGAELFQMLETGALQL
EEEEECCCCCCCCCHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCC
PPIEVRSFDALPLTLTDLKSRAHKGKLVVVT
CCCCCCCCCCCCEEHHHHHHHHCCCCEEEEC
>Mature Secondary Structure
MIQSLPEKHQVWAWPDKGEPQSLRLEIRTRPIPTPGEVLVANSAIGLNPVDWKMIEWGHA
CCCCCCCCCCEEECCCCCCCCEEEEEEEECCCCCCCCEEEECCCCCCCCCCEEEEECCCH
AWSAGHVPGVDGVGVIAATGDGVRLPIGLRGAYHQSLARDGSFAEFTIIDARSILPIPAS
HCCCCCCCCCCCCEEEEECCCCEEEEEECCHHHHHHHHCCCCCEEEEEEECCCCCCCCHH
VADVVAAALPCPGLTAWQALRKVPRRMVRDVLVTGAGGSVGSILAQLAARCGWRVWVTAA
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHCCCEEEEEEC
SAHRDKLLALGITGVFDYRDPNWCDRLVESLGPRRLYAIFDTVSGEHAQTLAPMLGYNGH
CCCCCEEEEEEEEEEECCCCCHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHCCCCC
LICIQDRIEENPLPAFTTAVSLHEVALNSVHLNATDEDWQEWRFAGAELFQMLETGALQL
EEEEECCCCCCCCCHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCC
PPIEVRSFDALPLTLTDLKSRAHKGKLVVVT
CCCCCCCCCCCCEEHHHHHHHHCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.017 {9,10-phenanthrenequinone}} 0.0153 {2,6-dichlorophenolindophenol}} 0.013 {2,6-dichlorophenolindophenol}} 0.26 {1,4-naphthoquinone}} 0.065 {1,4-benzoquinone}} 0.007 {NADPH}} 0.0069 {NADPH}} 0.0024 {1,2-naphthoquinone}} [C]

Substrates: NADPH; Quinone [C]

Specific reaction: NADPH + Quinone = NADP(+) + Semiquinone. [C]

General reaction: Oxidation; Reduction [C]

Inhibitor: 2, 3-Dimercaptopropanol; 2, 5-Dichloro-3, 6-dihydroxy -1, 4-benzoquinone; 4-Hydroxy coumarin; 5, 5'-Dithiobis (2-nitrobenzoate); ADP; Cibacronblue3GA; Coumarin; Cu2+; Dicoumarol warfarin, coumarin; Dithiothreitol preincubation with 9, 10-phenanthrenequino

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]