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The map label for this gene is miaA

Identifier: 182679765

GI number: 182679765

Start: 3253192

End: 3254100

Strand: Reverse

Name: miaA

Synonym: Bind_2853

Alternate gene names: 182679765

Gene position: 3254100-3253192 (Counterclockwise)

Preceding gene: 182679767

Following gene: 182679764

Centisome position: 78.03

GC content: 60.51

Gene sequence:

>909_bases
ATGGCTCTGCTCATTGCCGGTCCGACGGCGAGCGGCAAATCGGCCCTGGCGCTGCGGCTTGCTGAAACCTGCAACGGCGT
TGTGATCAATACCGATTCCATGCAGCTCTATCGTGACTTGCATGTGCTGACGGCAAGGCCAACTCAGGAAGAAGAACATC
AGGCGCCGCATGTCCTGTTTGGGAGCGTCGATGGCCGAATCAATTATTCCGTCGCGCTTTGGCTCGATCAGGCAAGGATC
GCCTTGGATGAGGCGCAAGGCCAAGGGCGCATGCCGATCTTCGTTGGCGGTACGGGGCTCTATTTCAAGGCGTTGACGCA
GGGGCTTTCCGATATACCGTCCGTTCCCGACAGCATACGTGCCGATTTGCGCCGACATGCGGAAGGGTGCTCCACGCCTG
AACTGCACCGTGAATTGGCGGCCCGCGATCCAGAAATGGCGGCACGGCTGAAACCCAATGACAGCCAGCGTATTTTGCGG
GCGCTGGAGGTGTTTGTCGCCACGGGCCAGAGTCTCGCAGCCTTTCAGAACGCGCGAAGCGCCCCCCTGCTGACCATGTC
CGAGGTTCTCGCGGTTTTTCTCGCGCCGGATCGGGCCGATCTCAATCGGCGGATCGATAGGCGTTTCGACACCATGCTGG
AGCAGGGCGCCTTGCAAGAAGTCGAGCGGCTAGGCGCGCGCGGACTCGACCCGAGTCTGCCTGTCATGCGGGCGCATGGC
GTGCCGCATCTTTTGCGGGCTCTGCGGGGGGAAATCAGCCTTGCGGAAGCGGCTGATCGCGGCAAACTTGATACGCGGCA
TTATGCCAAGCGTCAGTTCACCTTCGCGCGGCATCAATTGCCGGACTTTGCCTGGAGGACCCCGGAAGAAGCCGAGACTT
TTTTTCTGTCCAAGCTTCAGCGGATGTAA

Upstream 100 bases:

>100_bases
AAAATGGATTGCCAAGATCGAAAGCGGATTTTTTGCGTCTCATGCCCCAAAGATCGCGCTCGTTTCATCATTTGAGTCCG
GAACGATCATGAGCGCACCA

Downstream 100 bases:

>100_bases
AGTTCTGAAAATCTTTTACAAAGCGATTATTACAGCATTTATGCCTGTGCGGATAAAATGGTTTCGTTGTTCTTACGGCA
CCGCTCAATCGACTATCTGT

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 302; Mature: 301

Protein sequence:

>302_residues
MALLIAGPTASGKSALALRLAETCNGVVINTDSMQLYRDLHVLTARPTQEEEHQAPHVLFGSVDGRINYSVALWLDQARI
ALDEAQGQGRMPIFVGGTGLYFKALTQGLSDIPSVPDSIRADLRRHAEGCSTPELHRELAARDPEMAARLKPNDSQRILR
ALEVFVATGQSLAAFQNARSAPLLTMSEVLAVFLAPDRADLNRRIDRRFDTMLEQGALQEVERLGARGLDPSLPVMRAHG
VPHLLRALRGEISLAEAADRGKLDTRHYAKRQFTFARHQLPDFAWRTPEEAETFFLSKLQRM

Sequences:

>Translated_302_residues
MALLIAGPTASGKSALALRLAETCNGVVINTDSMQLYRDLHVLTARPTQEEEHQAPHVLFGSVDGRINYSVALWLDQARI
ALDEAQGQGRMPIFVGGTGLYFKALTQGLSDIPSVPDSIRADLRRHAEGCSTPELHRELAARDPEMAARLKPNDSQRILR
ALEVFVATGQSLAAFQNARSAPLLTMSEVLAVFLAPDRADLNRRIDRRFDTMLEQGALQEVERLGARGLDPSLPVMRAHG
VPHLLRALRGEISLAEAADRGKLDTRHYAKRQFTFARHQLPDFAWRTPEEAETFFLSKLQRM
>Mature_301_residues
ALLIAGPTASGKSALALRLAETCNGVVINTDSMQLYRDLHVLTARPTQEEEHQAPHVLFGSVDGRINYSVALWLDQARIA
LDEAQGQGRMPIFVGGTGLYFKALTQGLSDIPSVPDSIRADLRRHAEGCSTPELHRELAARDPEMAARLKPNDSQRILRA
LEVFVATGQSLAAFQNARSAPLLTMSEVLAVFLAPDRADLNRRIDRRFDTMLEQGALQEVERLGARGLDPSLPVMRAHGV
PHLLRALRGEISLAEAADRGKLDTRHYAKRQFTFARHQLPDFAWRTPEEAETFFLSKLQRM

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=232, Percent_Identity=31.0344827586207, Blast_Score=112, Evalue=6e-25,
Organism=Escherichia coli, GI1790613, Length=277, Percent_Identity=40.4332129963899, Blast_Score=172, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI25144712, Length=299, Percent_Identity=25.752508361204, Blast_Score=80, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6324848, Length=295, Percent_Identity=30.8474576271186, Blast_Score=139, Evalue=7e-34,
Organism=Drosophila melanogaster, GI24649830, Length=239, Percent_Identity=32.6359832635983, Blast_Score=112, Evalue=4e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_BEII9 (B2IKE2)

Other databases:

- EMBL:   CP001016
- RefSeq:   YP_001833911.1
- ProteinModelPortal:   B2IKE2
- GeneID:   6198729
- GenomeReviews:   CP001016_GR
- KEGG:   bid:Bind_2853
- HOGENOM:   HBG559459
- OMA:   VNADSMQ
- ProtClustDB:   CLSK2405781
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 33507; Mature: 33376

Theoretical pI: Translated: 7.77; Mature: 7.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALLIAGPTASGKSALALRLAETCNGVVINTDSMQLYRDLHVLTARPTQEEEHQAPHVLF
CEEEEECCCCCCCHHHHHHHHHHCCCEEEECCHHHHHHHHHHEECCCCCHHHHCCCEEEE
GSVDGRINYSVALWLDQARIALDEAQGQGRMPIFVGGTGLYFKALTQGLSDIPSVPDSIR
ECCCCEEEEEEEEEEHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHHHCCCCCHHHH
ADLRRHAEGCSTPELHRELAARDPEMAARLKPNDSQRILRALEVFVATGQSLAAFQNARS
HHHHHHHCCCCCHHHHHHHHHCCHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHCCCC
APLLTMSEVLAVFLAPDRADLNRRIDRRFDTMLEQGALQEVERLGARGLDPSLPVMRAHG
CCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCC
VPHLLRALRGEISLAEAADRGKLDTRHYAKRQFTFARHQLPDFAWRTPEEAETFFLSKLQ
HHHHHHHHHCCCHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
RM
CC
>Mature Secondary Structure 
ALLIAGPTASGKSALALRLAETCNGVVINTDSMQLYRDLHVLTARPTQEEEHQAPHVLF
EEEEECCCCCCCHHHHHHHHHHCCCEEEECCHHHHHHHHHHEECCCCCHHHHCCCEEEE
GSVDGRINYSVALWLDQARIALDEAQGQGRMPIFVGGTGLYFKALTQGLSDIPSVPDSIR
ECCCCEEEEEEEEEEHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHHHCCCCCHHHH
ADLRRHAEGCSTPELHRELAARDPEMAARLKPNDSQRILRALEVFVATGQSLAAFQNARS
HHHHHHHCCCCCHHHHHHHHHCCHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHCCCC
APLLTMSEVLAVFLAPDRADLNRRIDRRFDTMLEQGALQEVERLGARGLDPSLPVMRAHG
CCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCC
VPHLLRALRGEISLAEAADRGKLDTRHYAKRQFTFARHQLPDFAWRTPEEAETFFLSKLQ
HHHHHHHHHCCCHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
RM
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA