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The map label for this gene is camA [H]

Identifier: 182679762

GI number: 182679762

Start: 3249175

End: 3250419

Strand: Reverse

Name: camA [H]

Synonym: Bind_2850

Alternate gene names: 182679762

Gene position: 3250419-3249175 (Counterclockwise)

Preceding gene: 182679763

Following gene: 182679759

Centisome position: 77.94

GC content: 61.04

Gene sequence:

>1245_bases
ATGGTGGATCAGTCCGATCCGCGTCAGACGCGGATCGTTGTCGTTGGCGCGGGACATGCCGGCGGCACTTTTGTCAGCTT
GATGCGTGAGATGGGGCATGAGGGGCCAATCCTTGTGATTGGCGAGGAAACGGCTGCTCCCTATCAGAGACCACCCCTTT
CCAAGGACTATCTCAAGGGCAATCTCGCCGAGGACTCTTTGTTTCTGCGTGCTCCTTCTTTCTATGAGGAGCGCAAGATC
ATCGTACGGACGGGCGAGAGCGTGGAGAGGATCGATCGCGAGGAAAAAGCGATCCGTCTCGCGGGTGGTGACGTCGAGCC
CTATGATGTGCTGGTCTTGGCCACAGGGTCCGAAAACCGTCGTTTGGGCGTCGAGGGAGCAGATCTTACCAATATTTTTG
GACTGCGGACGCTGGCTGAGGCCGGTCTCTTGAAACAGGTCCTGCGGCCGGAAAGCCGCCTCGCTGTCATCGGTGGCGGC
TATGTGGGTCTTGAGGTCGCGGCCTCCGCCCGCCTGCTCGGTGCCGATGTCGTGGTGATTGAGCGCGAGCCCCGGGTTCT
GGCCCGCGTTGCTTGCGAGCCTTTGTCACGGTTCTATGAATCCCATCATCGCGCCCAGGGCGTCCGCATTGAAACCGGGG
CGCAGGTGACGGGCTTCGAAGGGGATGCCGGAAGCATTGCCGGGGTCAGGCTGGCGGATGCGCGGCAATTTGCCTGCGAT
GTCGCAATCGTAGGCATCGGGGCCGTGGCGCGCGATAGGCTGGCGCGGGAAGCTGGCCTTGCCTGCGATAATGGCGTACG
CGTCGATCTCGATGCCAGAACCTCCGATCCGTCCATCTATGCCTTGGGCGATGTGACCTTGCGTCCGTTGCCGCTTTATC
AGGATCGCATGGCCCGTCTCGAAAGCGTGGCCAATGCGCTGGAGCAGGCCAAACAAGCGGCAGCTTCCATTCTCGGCCAG
CCACGGCCGGAACCCGTGGTGCCGTGGTTCTGGTCGGATCAATATGACGTCAAATTGCAGATTGCCGGCATGCCCTTCGA
CTGCGACGACATGGTGATCCGTGGTGCGATCGACAGCGGCAAATTCGCGATCTTTCATATGCGGGGCGATCACATCCAGG
CGGTCGAGGCGGTCAATGCCGCGCCCGAATTCATGGGTGGCCGTCTGCTCATCGCCTCGCAGCAGAAAGTGGATCGGGCA
AAGCTTGCCGATCCCACCGTCAGTATCAAGAGGGTGGCGGTTTAG

Upstream 100 bases:

>100_bases
TTCGGATAAATCCAAAATGCTCGAGGCAGCACTGATTTTGCTTTGAAACCGAAATCAATCGTCGTTTGAACTACCGAATT
GGTTGGGTCGCGAGGGTAAC

Downstream 100 bases:

>100_bases
GCAGGCTGAATGGTGTGATCTTCCGCCTGCCTTATGACTTTGTCATTTGGTGCCGTGGGCGGCAGTTTGCGCGAAAAACG
AAGCATGATTGGCTCTTCGA

Product: FAD-dependent pyridine nucleotide-disulfide oxidoreductase

Products: oxidized ferredoxin; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 414; Mature: 414

Protein sequence:

>414_residues
MVDQSDPRQTRIVVVGAGHAGGTFVSLMREMGHEGPILVIGEETAAPYQRPPLSKDYLKGNLAEDSLFLRAPSFYEERKI
IVRTGESVERIDREEKAIRLAGGDVEPYDVLVLATGSENRRLGVEGADLTNIFGLRTLAEAGLLKQVLRPESRLAVIGGG
YVGLEVAASARLLGADVVVIEREPRVLARVACEPLSRFYESHHRAQGVRIETGAQVTGFEGDAGSIAGVRLADARQFACD
VAIVGIGAVARDRLAREAGLACDNGVRVDLDARTSDPSIYALGDVTLRPLPLYQDRMARLESVANALEQAKQAAASILGQ
PRPEPVVPWFWSDQYDVKLQIAGMPFDCDDMVIRGAIDSGKFAIFHMRGDHIQAVEAVNAAPEFMGGRLLIASQQKVDRA
KLADPTVSIKRVAV

Sequences:

>Translated_414_residues
MVDQSDPRQTRIVVVGAGHAGGTFVSLMREMGHEGPILVIGEETAAPYQRPPLSKDYLKGNLAEDSLFLRAPSFYEERKI
IVRTGESVERIDREEKAIRLAGGDVEPYDVLVLATGSENRRLGVEGADLTNIFGLRTLAEAGLLKQVLRPESRLAVIGGG
YVGLEVAASARLLGADVVVIEREPRVLARVACEPLSRFYESHHRAQGVRIETGAQVTGFEGDAGSIAGVRLADARQFACD
VAIVGIGAVARDRLAREAGLACDNGVRVDLDARTSDPSIYALGDVTLRPLPLYQDRMARLESVANALEQAKQAAASILGQ
PRPEPVVPWFWSDQYDVKLQIAGMPFDCDDMVIRGAIDSGKFAIFHMRGDHIQAVEAVNAAPEFMGGRLLIASQQKVDRA
KLADPTVSIKRVAV
>Mature_414_residues
MVDQSDPRQTRIVVVGAGHAGGTFVSLMREMGHEGPILVIGEETAAPYQRPPLSKDYLKGNLAEDSLFLRAPSFYEERKI
IVRTGESVERIDREEKAIRLAGGDVEPYDVLVLATGSENRRLGVEGADLTNIFGLRTLAEAGLLKQVLRPESRLAVIGGG
YVGLEVAASARLLGADVVVIEREPRVLARVACEPLSRFYESHHRAQGVRIETGAQVTGFEGDAGSIAGVRLADARQFACD
VAIVGIGAVARDRLAREAGLACDNGVRVDLDARTSDPSIYALGDVTLRPLPLYQDRMARLESVANALEQAKQAAASILGQ
PRPEPVVPWFWSDQYDVKLQIAGMPFDCDDMVIRGAIDSGKFAIFHMRGDHIQAVEAVNAAPEFMGGRLLIASQQKVDRA
KLADPTVSIKRVAV

Specific function: The oxidation of camphor by cytochrome P450-CAM requires the participation of a flavoprotein, putidaredoxin reductase, and an iron-sulfur protein, putidaredoxin, to mediate the transfer of electrons from NADH to P450 for oxygen activation [H]

COG id: COG0446

COG function: function code R; Uncharacterized NAD(FAD)-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI21389617, Length=375, Percent_Identity=28.8, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI65787454, Length=375, Percent_Identity=28.8, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI226437568, Length=375, Percent_Identity=28.8, Blast_Score=153, Evalue=2e-37,
Organism=Homo sapiens, GI22202629, Length=337, Percent_Identity=26.7062314540059, Blast_Score=102, Evalue=5e-22,
Organism=Homo sapiens, GI4757732, Length=337, Percent_Identity=26.7062314540059, Blast_Score=102, Evalue=5e-22,
Organism=Escherichia coli, GI1788892, Length=408, Percent_Identity=33.3333333333333, Blast_Score=174, Evalue=8e-45,
Organism=Escherichia coli, GI1789065, Length=273, Percent_Identity=29.3040293040293, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI1789765, Length=321, Percent_Identity=31.7757009345794, Blast_Score=95, Evalue=9e-21,
Organism=Caenorhabditis elegans, GI17559934, Length=387, Percent_Identity=29.9741602067183, Blast_Score=174, Evalue=6e-44,
Organism=Drosophila melanogaster, GI24639257, Length=412, Percent_Identity=27.4271844660194, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI281359715, Length=412, Percent_Identity=27.4271844660194, Blast_Score=123, Evalue=3e-28,
Organism=Drosophila melanogaster, GI281359713, Length=412, Percent_Identity=27.4271844660194, Blast_Score=123, Evalue=3e-28,
Organism=Drosophila melanogaster, GI24639250, Length=412, Percent_Identity=27.4271844660194, Blast_Score=123, Evalue=3e-28,
Organism=Drosophila melanogaster, GI18543267, Length=412, Percent_Identity=27.4271844660194, Blast_Score=123, Evalue=3e-28,
Organism=Drosophila melanogaster, GI24639252, Length=412, Percent_Identity=27.4271844660194, Blast_Score=123, Evalue=3e-28,
Organism=Drosophila melanogaster, GI24585130, Length=403, Percent_Identity=22.3325062034739, Blast_Score=102, Evalue=6e-22,
Organism=Drosophila melanogaster, GI24581020, Length=366, Percent_Identity=23.7704918032787, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI28573993, Length=366, Percent_Identity=23.7704918032787, Blast_Score=86, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR004099
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.18.1.3

Molecular weight: Translated: 44743; Mature: 44743

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVDQSDPRQTRIVVVGAGHAGGTFVSLMREMGHEGPILVIGEETAAPYQRPPLSKDYLKG
CCCCCCCCCEEEEEEECCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCHHHHCC
NLAEDSLFLRAPSFYEERKIIVRTGESVERIDREEKAIRLAGGDVEPYDVLVLATGSENR
CCCCCCEEEECCCCHHCCEEEEECCCHHHHHCCHHHEEEEECCCCCCEEEEEEEECCCCC
RLGVEGADLTNIFGLRTLAEAGLLKQVLRPESRLAVIGGGYVGLEVAASARLLGADVVVI
EECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCEEEEEEHHCCEEECCEEEEE
EREPRVLARVACEPLSRFYESHHRAQGVRIETGAQVTGFEGDAGSIAGVRLADARQFACD
ECCCHHHHHHHHHHHHHHHHHHHHHCCEEEECCCEEECCCCCCCCEEEEEEECCHHHHEE
VAIVGIGAVARDRLAREAGLACDNGVRVDLDARTSDPSIYALGDVTLRPLPLYQDRMARL
EEEEECCHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCEEEECCCEECCCCCHHHHHHHH
ESVANALEQAKQAAASILGQPRPEPVVPWFWSDQYDVKLQIAGMPFDCDDMVIRGAIDSG
HHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCEEEEEEEECCCCCCCCCEEEEECCCC
KFAIFHMRGDHIQAVEAVNAAPEFMGGRLLIASQQKVDRAKLADPTVSIKRVAV
CEEEEEECCCCCHHHHHHHCCHHHCCCEEEEECCCHHHHHHCCCCCCEEEEECC
>Mature Secondary Structure
MVDQSDPRQTRIVVVGAGHAGGTFVSLMREMGHEGPILVIGEETAAPYQRPPLSKDYLKG
CCCCCCCCCEEEEEEECCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCHHHHCC
NLAEDSLFLRAPSFYEERKIIVRTGESVERIDREEKAIRLAGGDVEPYDVLVLATGSENR
CCCCCCEEEECCCCHHCCEEEEECCCHHHHHCCHHHEEEEECCCCCCEEEEEEEECCCCC
RLGVEGADLTNIFGLRTLAEAGLLKQVLRPESRLAVIGGGYVGLEVAASARLLGADVVVI
EECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCEEEEEEHHCCEEECCEEEEE
EREPRVLARVACEPLSRFYESHHRAQGVRIETGAQVTGFEGDAGSIAGVRLADARQFACD
ECCCHHHHHHHHHHHHHHHHHHHHHCCEEEECCCEEECCCCCCCCEEEEEEECCHHHHEE
VAIVGIGAVARDRLAREAGLACDNGVRVDLDARTSDPSIYALGDVTLRPLPLYQDRMARL
EEEEECCHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCEEEECCCEECCCCCHHHHHHHH
ESVANALEQAKQAAASILGQPRPEPVVPWFWSDQYDVKLQIAGMPFDCDDMVIRGAIDSG
HHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCEEEEEEEECCCCCCCCCEEEEECCCC
KFAIFHMRGDHIQAVEAVNAAPEFMGGRLLIASQQKVDRAKLADPTVSIKRVAV
CEEEEEECCCCCHHHHHHHCCHHHCCCEEEEECCCHHHHHHCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: reduced ferredoxin; NAD+

Specific reaction: reduced ferredoxin + NAD+ = oxidized ferredoxin + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2180940; 2613690; 3003058; 12011076; 11524002 [H]