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The map label for this gene is garL [H]

Identifier: 182679742

GI number: 182679742

Start: 3224943

End: 3225764

Strand: Reverse

Name: garL [H]

Synonym: Bind_2830

Alternate gene names: 182679742

Gene position: 3225764-3224943 (Counterclockwise)

Preceding gene: 182679743

Following gene: 182679741

Centisome position: 77.35

GC content: 62.04

Gene sequence:

>822_bases
ATGAGACCCAATGCGATCAGGCAATCCTTTGCCGCCGGCAAGGCTGTCATCAATGGATGGCTTTCCATAGCCTGCCCGTT
CACCGCCGAGATCATGGCACATCAGGGCTATGATTCCATCACCATCGATCTCCAGCATGGCCTCGTCGGCTATGAGGTGG
CGACAACCATGCTCCAGGCCATGCAGGCAAGTTCGGTCACGCCGATGGTGCGTGTGCCCTGGCTTGATCCGGGCGCTATC
ATGAAGGCGCTGGACGCCGGAGCCTATGGCGTGATCTGCCCCATGATCAATACGCCGAAAGAGGCTGAAAAGCTCGTCTC
CTGTGTGCGTTATCCACCCAACGGCACGCGCAGCTTCGGCCCGACGCGCGTCTCCATCGCTTCGGGCGCCGATTATGGCG
AGCATGCAGACCGTGAAGTTCTGTGCTTCGCCATGATCGAGACGGCGCAGGCCTTCGCCAATCTCGATGCCATCGCCGCG
ACGCCGGGGCTCGATGGCCTTTATATTGGTCCCGCGGACCTGACGCTCGGGCTCACCGGCCGCCAGTACCGCACCGGTTT
CGACCGCGAGGAGCCGGAAATGGTCGCCGCGATCAAGACCATTCTCGAGGCGGCGCACAAGGCCGGCATCCGCGCCTGCC
TGCATAATGGCACGCCGACTTATGCCGCCAAGGCCATCAGCTGGGGTTTTGATCTCGTCACCATCTCCAATGACGTGCGG
CTGCTTGCTGGCGCGGCGCAGGCATCGGTGGCGACGGCGCGTCAGCTCATCGGCCAATTGACCAGTGTGCCCGATCTTAA
ACAGGCGGCGGGAGGATATTGA

Upstream 100 bases:

>100_bases
GCATCAAGTTCAGCGGCATGGGCCGCGAGGGCGGAAGTGAGGGAATCCTCGACTTCCTGAACGTCAAACTTGCCCAGGTG
GCCGTGTGAGGATCTGACCC

Downstream 100 bases:

>100_bases
AATGACGCATATTCTTGTCGCCGGACGCATACATGAAGCAGGGCTCGACATCCTGCGCAATGCATCAGGTATCACGCTGG
ATTTAGTGGATGAAGTCTCG

Product: HpcH/HpaI aldolase

Products: NA

Alternate protein names: KDGluc aldolase; KDGlucA; 2-dehydro-3-deoxy-D-glucarate aldolase; 2-keto-3-deoxy-D-glucarate aldolase; 5-dehydro-4-deoxy-D-glucarate aldolase; Alpha-keto-beta-deoxy-D-glucarate aldolase [H]

Number of amino acids: Translated: 273; Mature: 273

Protein sequence:

>273_residues
MRPNAIRQSFAAGKAVINGWLSIACPFTAEIMAHQGYDSITIDLQHGLVGYEVATTMLQAMQASSVTPMVRVPWLDPGAI
MKALDAGAYGVICPMINTPKEAEKLVSCVRYPPNGTRSFGPTRVSIASGADYGEHADREVLCFAMIETAQAFANLDAIAA
TPGLDGLYIGPADLTLGLTGRQYRTGFDREEPEMVAAIKTILEAAHKAGIRACLHNGTPTYAAKAISWGFDLVTISNDVR
LLAGAAQASVATARQLIGQLTSVPDLKQAAGGY

Sequences:

>Translated_273_residues
MRPNAIRQSFAAGKAVINGWLSIACPFTAEIMAHQGYDSITIDLQHGLVGYEVATTMLQAMQASSVTPMVRVPWLDPGAI
MKALDAGAYGVICPMINTPKEAEKLVSCVRYPPNGTRSFGPTRVSIASGADYGEHADREVLCFAMIETAQAFANLDAIAA
TPGLDGLYIGPADLTLGLTGRQYRTGFDREEPEMVAAIKTILEAAHKAGIRACLHNGTPTYAAKAISWGFDLVTISNDVR
LLAGAAQASVATARQLIGQLTSVPDLKQAAGGY
>Mature_273_residues
MRPNAIRQSFAAGKAVINGWLSIACPFTAEIMAHQGYDSITIDLQHGLVGYEVATTMLQAMQASSVTPMVRVPWLDPGAI
MKALDAGAYGVICPMINTPKEAEKLVSCVRYPPNGTRSFGPTRVSIASGADYGEHADREVLCFAMIETAQAFANLDAIAA
TPGLDGLYIGPADLTLGLTGRQYRTGFDREEPEMVAAIKTILEAAHKAGIRACLHNGTPTYAAKAISWGFDLVTISNDVR
LLAGAAQASVATARQLIGQLTSVPDLKQAAGGY

Specific function: Catalyzes the reversible retro-aldol cleavage of both 5- keto-4-deoxy-D-glucarate and 2-keto-3-deoxy-D-glucarate to pyruvate and tartronic semialdehyde [H]

COG id: COG3836

COG function: function code G; 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family. KDGluc aldolase subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789514, Length=257, Percent_Identity=29.5719844357977, Blast_Score=128, Evalue=4e-31,
Organism=Escherichia coli, GI1788578, Length=254, Percent_Identity=31.1023622047244, Blast_Score=110, Evalue=7e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR017648
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: =4.1.2.20 [H]

Molecular weight: Translated: 28856; Mature: 28856

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPNAIRQSFAAGKAVINGWLSIACPFTAEIMAHQGYDSITIDLQHGLVGYEVATTMLQA
CCCHHHHHHHHHHHHHHHHHHHEECCHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHH
MQASSVTPMVRVPWLDPGAIMKALDAGAYGVICPMINTPKEAEKLVSCVRYPPNGTRSFG
HHHCCCCCEEEECCCCHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHCCCCCCCCCCC
PTRVSIASGADYGEHADREVLCFAMIETAQAFANLDAIAATPGLDGLYIGPADLTLGLTG
CCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHEECCCCCEEEECCCCEEEECCC
RQYRTGFDREEPEMVAAIKTILEAAHKAGIRACLHNGTPTYAAKAISWGFDLVTISNDVR
CHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCEEEEEECCCCE
LLAGAAQASVATARQLIGQLTSVPDLKQAAGGY
EEHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCC
>Mature Secondary Structure
MRPNAIRQSFAAGKAVINGWLSIACPFTAEIMAHQGYDSITIDLQHGLVGYEVATTMLQA
CCCHHHHHHHHHHHHHHHHHHHEECCHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHH
MQASSVTPMVRVPWLDPGAIMKALDAGAYGVICPMINTPKEAEKLVSCVRYPPNGTRSFG
HHHCCCCCEEEECCCCHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHCCCCCCCCCCC
PTRVSIASGADYGEHADREVLCFAMIETAQAFANLDAIAATPGLDGLYIGPADLTLGLTG
CCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHEECCCCCEEEECCCCEEEECCC
RQYRTGFDREEPEMVAAIKTILEAAHKAGIRACLHNGTPTYAAKAISWGFDLVTISNDVR
CHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCEEEEEECCCCE
LLAGAAQASVATARQLIGQLTSVPDLKQAAGGY
EEHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA