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The map label for this gene is afr [H]

Identifier: 182679739

GI number: 182679739

Start: 3221536

End: 3222657

Strand: Reverse

Name: afr [H]

Synonym: Bind_2827

Alternate gene names: 182679739

Gene position: 3222657-3221536 (Counterclockwise)

Preceding gene: 182679740

Following gene: 182679738

Centisome position: 77.28

GC content: 59.8

Gene sequence:

>1122_bases
ATGCGTGACATCGGCGTTGGTGTGATCGGCACCGGCTTCATGGGCAAGGCACATGCGATGGCCTATCGCGCCGTCGCCGG
TATCTTCCCGGGCAATTTGACACCTGTCCTGCAGGCGGTCGCAGATATCGATGGAGCCGCCGCCGAGGCGGCGGCGCGGC
AATATGGCTTTCAGCGCGCTTTGACAAACTGGCATGATCTCATCGCCGATCCGGCGGTGGAGATCGTCGCTATTACCACT
CCGAATACATTTCATCGGGAAATGGCTCTCGCTGCCATCGCTGCCGGCAAGCATGTTCATTGCGAAAAGCCACTTGCTCC
TTCCGTCGCGGAAGCGCGTGAGATGATGGAAGCATCCGAAGCGGCCAAGGTCGTCACACAGGTCGGGTATAATTACATCA
AAAATCCGTTGCTGTCGTTGGCGCGTGACATGATCGCGGCAGGCGAACTCGGCGAGATCACCAATTTTCGCGGCATACAT
GCCGAAGATTTCATGTGCGATCCTGATGTGCCCTATAGCTGGCGGCTCGATCCGCAGGGCGGCGGCGGAGCCATTGCCGA
TATCGGTAGCCATATTATCGGCCTCGTGCGTTTTCTTCTTGGCCCGATTACCGAAGTGAATGCCGATCTTGCGACCGTGG
TGAAAAGCCGTCCGGTCGCGCCGGGTGCGACGGAACGCCGTCTCATCGAGGTCGATGACATTGCCCGCCTCGCTTTGCGC
TTCGCGCGTGGCTGTGGCGGCACCATCGAGGCCAATTGGGCGGCGACCGGCCGCAAGATGCAGCTCGGCTTTGAGCTTTC
GGGCACGAAAGGCGCGTTGGTCTTCACGCAGGAACGCCTCAACGAACTCCTTTATTACAAAACCGGCGAGGATCCGCGTC
AAAGCGGCTTCCAGCGTATCGAGGCTGGTCCGCAGCATCCGCCCTATCGCAACTTCTGCGTGGCACCCGGCCATCAGCTC
GGCTTCAACGATTTGAAAACGATCGAGGTCGCCGATTTCCTCGCAGCCATCGACGGTGGGGAGCGGCGCGGACCGGACTT
CCGCGAAGCTTGGGAAATTCAGAAGGTCGTCGATACAGCAATCACATCGAGCCGGGCCCGCGCATGGAAGGAGGTTGCGT
AA

Upstream 100 bases:

>100_bases
GCTTGCCGATTGCGCCCTTGAATCGGTTCTCACGGGCCACGCCGTGAAAGTTTGAATCCATAACCAGACATTGACAGAAA
ATATCGAGGAAGACAGTCTC

Downstream 100 bases:

>100_bases
TGTCATCCATGTCCGATCGCAACGCGGCCAAGGCGCCGCTTGGTGGACCTCAGGCGGCTAAGGCCATGCGCACCATCAAG
GGACCCGCGGTTTTTCTCGC

Product: oxidoreductase domain-containing protein

Products: NA

Alternate protein names: Anhydrofructose reductase; 1,5-anhydro-D-fructose reductase (1,5-anhydro-D-mannitol-forming) [H]

Number of amino acids: Translated: 373; Mature: 373

Protein sequence:

>373_residues
MRDIGVGVIGTGFMGKAHAMAYRAVAGIFPGNLTPVLQAVADIDGAAAEAAARQYGFQRALTNWHDLIADPAVEIVAITT
PNTFHREMALAAIAAGKHVHCEKPLAPSVAEAREMMEASEAAKVVTQVGYNYIKNPLLSLARDMIAAGELGEITNFRGIH
AEDFMCDPDVPYSWRLDPQGGGGAIADIGSHIIGLVRFLLGPITEVNADLATVVKSRPVAPGATERRLIEVDDIARLALR
FARGCGGTIEANWAATGRKMQLGFELSGTKGALVFTQERLNELLYYKTGEDPRQSGFQRIEAGPQHPPYRNFCVAPGHQL
GFNDLKTIEVADFLAAIDGGERRGPDFREAWEIQKVVDTAITSSRARAWKEVA

Sequences:

>Translated_373_residues
MRDIGVGVIGTGFMGKAHAMAYRAVAGIFPGNLTPVLQAVADIDGAAAEAAARQYGFQRALTNWHDLIADPAVEIVAITT
PNTFHREMALAAIAAGKHVHCEKPLAPSVAEAREMMEASEAAKVVTQVGYNYIKNPLLSLARDMIAAGELGEITNFRGIH
AEDFMCDPDVPYSWRLDPQGGGGAIADIGSHIIGLVRFLLGPITEVNADLATVVKSRPVAPGATERRLIEVDDIARLALR
FARGCGGTIEANWAATGRKMQLGFELSGTKGALVFTQERLNELLYYKTGEDPRQSGFQRIEAGPQHPPYRNFCVAPGHQL
GFNDLKTIEVADFLAAIDGGERRGPDFREAWEIQKVVDTAITSSRARAWKEVA
>Mature_373_residues
MRDIGVGVIGTGFMGKAHAMAYRAVAGIFPGNLTPVLQAVADIDGAAAEAAARQYGFQRALTNWHDLIADPAVEIVAITT
PNTFHREMALAAIAAGKHVHCEKPLAPSVAEAREMMEASEAAKVVTQVGYNYIKNPLLSLARDMIAAGELGEITNFRGIH
AEDFMCDPDVPYSWRLDPQGGGGAIADIGSHIIGLVRFLLGPITEVNADLATVVKSRPVAPGATERRLIEVDDIARLALR
FARGCGGTIEANWAATGRKMQLGFELSGTKGALVFTQERLNELLYYKTGEDPRQSGFQRIEAGPQHPPYRNFCVAPGHQL
GFNDLKTIEVADFLAAIDGGERRGPDFREAWEIQKVVDTAITSSRARAWKEVA

Specific function: Catalyzes the NADPH-specific reduction of 1,5-anhydro-D- fructose to 1,5-anhydro-D-mannitol. Also shows some activity against structurally related compounds such as 3-keto-1,5-anhydro- D-fructose, D-glucosone and D-xylosone. The enzyme cannot use NADH as

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gfo/idh/mocA family [H]

Homologues:

Organism=Homo sapiens, GI7657212, Length=186, Percent_Identity=29.0322580645161, Blast_Score=68, Evalue=2e-11,
Organism=Escherichia coli, GI87081947, Length=212, Percent_Identity=28.7735849056604, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1787574, Length=213, Percent_Identity=30.9859154929577, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI87082405, Length=277, Percent_Identity=25.9927797833935, Blast_Score=64, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6323975, Length=210, Percent_Identity=25.2380952380952, Blast_Score=71, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR000683
- InterPro:   IPR004104 [H]

Pfam domain/function: PF01408 GFO_IDH_MocA; PF02894 GFO_IDH_MocA_C [H]

EC number: =1.1.1.292 [H]

Molecular weight: Translated: 40231; Mature: 40231

Theoretical pI: Translated: 6.06; Mature: 6.06

Prosite motif: PS00228 TUBULIN_B_AUTOREG

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRDIGVGVIGTGFMGKAHAMAYRAVAGIFPGNLTPVLQAVADIDGAAAEAAARQYGFQRA
CCCCCCEEEECCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHH
LTNWHDLIADPAVEIVAITTPNTFHREMALAAIAAGKHVHCEKPLAPSVAEAREMMEASE
HHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHH
AAKVVTQVGYNYIKNPLLSLARDMIAAGELGEITNFRGIHAEDFMCDPDVPYSWRLDPQG
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCC
GGGAIADIGSHIIGLVRFLLGPITEVNADLATVVKSRPVAPGATERRLIEVDDIARLALR
CCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCEEECHHHHHHHHHH
FARGCGGTIEANWAATGRKMQLGFELSGTKGALVFTQERLNELLYYKTGEDPRQSGFQRI
HHHCCCCCEECCCCCCCCEEEEEEEECCCCCEEEEEHHHHHHHEEECCCCCHHHHHHHHH
EAGPQHPPYRNFCVAPGHQLGFNDLKTIEVADFLAAIDGGERRGPDFREAWEIQKVVDTA
HCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHH
ITSSRARAWKEVA
HHHHHHHHHHHCC
>Mature Secondary Structure
MRDIGVGVIGTGFMGKAHAMAYRAVAGIFPGNLTPVLQAVADIDGAAAEAAARQYGFQRA
CCCCCCEEEECCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHH
LTNWHDLIADPAVEIVAITTPNTFHREMALAAIAAGKHVHCEKPLAPSVAEAREMMEASE
HHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHH
AAKVVTQVGYNYIKNPLLSLARDMIAAGELGEITNFRGIHAEDFMCDPDVPYSWRLDPQG
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCC
GGGAIADIGSHIIGLVRFLLGPITEVNADLATVVKSRPVAPGATERRLIEVDDIARLALR
CCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCEEECHHHHHHHHHH
FARGCGGTIEANWAATGRKMQLGFELSGTKGALVFTQERLNELLYYKTGEDPRQSGFQRI
HHHCCCCCEECCCCCCCCEEEEEEEECCCCCEEEEEHHHHHHHEEECCCCCHHHHHHHHH
EAGPQHPPYRNFCVAPGHQLGFNDLKTIEVADFLAAIDGGERRGPDFREAWEIQKVVDTA
HCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHH
ITSSRARAWKEVA
HHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA