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The map label for this gene is yogA [H]

Identifier: 182679559

GI number: 182679559

Start: 2996670

End: 2997677

Strand: Reverse

Name: yogA [H]

Synonym: Bind_2633

Alternate gene names: 182679559

Gene position: 2997677-2996670 (Counterclockwise)

Preceding gene: 182679560

Following gene: 182679550

Centisome position: 71.88

GC content: 65.97

Gene sequence:

>1008_bases
ATGAAAGCTATCCAGGCTCATCCGCAGCAAGGGCTTGACGGCCTGACCTTGGTGGACCTTCCCGATCCCGGCGATCCCGG
ACCAAACGCCATCCGCGTGCGAGTGCATGCCACCTCCCTGAATTTCCACGACCTGGGTGTGGTGACCGGACGATTGCCGG
TCGCTGATGGACGTATCCCCATGGCTGACGGCGCTGGAGTGGTGGAGGCGGTCGGCTCCGAAATCACAGAGTTCAAGCCC
GGCGACCATGTCGTATCCACCTTCTTCCCGACCTGGCTGGACGGCGAGGCCACCCTCGCCGACTTCGCTACCACGCCGGG
CGATGGGATCGACGGCTACGCCCGGGAGGTGGTGATAGCACCGGCCACCTCCTTCACGCGCGCTCCAGCCGGCTGGAGCC
ACGCCGCCGCTGCGACCCTGACCACAGCCGGGGTGACCGCCTGGCGAGCCTTGGTGGTCAACGGCGGCCTTAAGGTGGGA
GACAGCGTGCTGGTACTCGGCACGGGTGGCGTGTCGATCTTCGCGCTGCAAATTGCTCGGGCCATGGGGGCCACGGTTAT
CGCGACCTCCTCCTCCGATGAAAAGCTGGAGCGGCTTCGCTCCATGGGTGCTGCGCACTGCATCAACTACCGCCGGGATG
AGGACTGGGGCGACCGGGTGCGGGCCCTGACGGGCGGTATCGGAGTGGATCACGTCGTGGAGGTGGGCGGCCCGGGCACG
TTGGCGCAATCGATCAAGGCTGCGCGTGTGAGCGGCCACATTTCACTGATCGGCGTGCTGACCGGGAGGGAGGGAGTTGT
GCCGACAGGCATGCTTATGGCTCGGCAGCAGCGCCTTCAGGGCCTGATCGTTGGCAGCCGCCGTCATCAGATGGACCTGA
TCCGCGGCATCGATGCCGTCCATGGCCACCCGGTGATCGACCGCTCCTTTCCGCTCGCTGCGATCGCGAAGGCCTTCCGG
CTCCAGGCGACTGGCGGCCATTTCGGCAAGATCGTCCTCGAGTTCTGA

Upstream 100 bases:

>100_bases
TCCTGGGCACGACCCAGACTTATCTCGGCGCCGTTCTGGTCGTCTCGGCGACCGCGACCGTCTTGCAGTGGCTGCCCTCA
CGCAGCCGAGGAGCACCCGC

Downstream 100 bases:

>100_bases
CAAACACAGTCCAGCAGGAACACGCCACCATGAAAGTTCTCCTCGTCTTCGCCCACCCGGAACGCCACTCCTTGGGGATT
GGAAATGAGTTCGGGCAACA

Product: alcohol dehydrogenase

Products: NADP(+); Semiquinone. [C]

Alternate protein names: NA

Number of amino acids: Translated: 335; Mature: 335

Protein sequence:

>335_residues
MKAIQAHPQQGLDGLTLVDLPDPGDPGPNAIRVRVHATSLNFHDLGVVTGRLPVADGRIPMADGAGVVEAVGSEITEFKP
GDHVVSTFFPTWLDGEATLADFATTPGDGIDGYAREVVIAPATSFTRAPAGWSHAAAATLTTAGVTAWRALVVNGGLKVG
DSVLVLGTGGVSIFALQIARAMGATVIATSSSDEKLERLRSMGAAHCINYRRDEDWGDRVRALTGGIGVDHVVEVGGPGT
LAQSIKAARVSGHISLIGVLTGREGVVPTGMLMARQQRLQGLIVGSRRHQMDLIRGIDAVHGHPVIDRSFPLAAIAKAFR
LQATGGHFGKIVLEF

Sequences:

>Translated_335_residues
MKAIQAHPQQGLDGLTLVDLPDPGDPGPNAIRVRVHATSLNFHDLGVVTGRLPVADGRIPMADGAGVVEAVGSEITEFKP
GDHVVSTFFPTWLDGEATLADFATTPGDGIDGYAREVVIAPATSFTRAPAGWSHAAAATLTTAGVTAWRALVVNGGLKVG
DSVLVLGTGGVSIFALQIARAMGATVIATSSSDEKLERLRSMGAAHCINYRRDEDWGDRVRALTGGIGVDHVVEVGGPGT
LAQSIKAARVSGHISLIGVLTGREGVVPTGMLMARQQRLQGLIVGSRRHQMDLIRGIDAVHGHPVIDRSFPLAAIAKAFR
LQATGGHFGKIVLEF
>Mature_335_residues
MKAIQAHPQQGLDGLTLVDLPDPGDPGPNAIRVRVHATSLNFHDLGVVTGRLPVADGRIPMADGAGVVEAVGSEITEFKP
GDHVVSTFFPTWLDGEATLADFATTPGDGIDGYAREVVIAPATSFTRAPAGWSHAAAATLTTAGVTAWRALVVNGGLKVG
DSVLVLGTGGVSIFALQIARAMGATVIATSSSDEKLERLRSMGAAHCINYRRDEDWGDRVRALTGGIGVDHVVEVGGPGT
LAQSIKAARVSGHISLIGVLTGREGVVPTGMLMARQQRLQGLIVGSRRHQMDLIRGIDAVHGHPVIDRSFPLAAIAKAFR
LQATGGHFGKIVLEF

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily [H]

Homologues:

Organism=Homo sapiens, GI22538446, Length=350, Percent_Identity=26, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI22538444, Length=350, Percent_Identity=26, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI194239674, Length=349, Percent_Identity=26.647564469914, Blast_Score=89, Evalue=7e-18,
Organism=Homo sapiens, GI13236495, Length=349, Percent_Identity=26.647564469914, Blast_Score=89, Evalue=7e-18,
Organism=Homo sapiens, GI47519420, Length=358, Percent_Identity=27.9329608938547, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI194239676, Length=341, Percent_Identity=26.3929618768328, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI18379349, Length=331, Percent_Identity=26.5861027190332, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI28557745, Length=245, Percent_Identity=28.5714285714286, Blast_Score=66, Evalue=4e-11,
Organism=Escherichia coli, GI1790485, Length=276, Percent_Identity=27.8985507246377, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI87081918, Length=313, Percent_Identity=28.7539936102236, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1788073, Length=239, Percent_Identity=27.1966527196653, Blast_Score=62, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6323729, Length=340, Percent_Identity=25.2941176470588, Blast_Score=74, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6322619, Length=339, Percent_Identity=25.9587020648968, Blast_Score=74, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6319955, Length=339, Percent_Identity=25.9587020648968, Blast_Score=74, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6323961, Length=317, Percent_Identity=25.5520504731861, Blast_Score=72, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6324486, Length=303, Percent_Identity=25.7425742574257, Blast_Score=70, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24581345, Length=221, Percent_Identity=29.8642533936652, Blast_Score=75, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: 1.6.5.5 [C]

Molecular weight: Translated: 34939; Mature: 34939

Theoretical pI: Translated: 7.18; Mature: 7.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAIQAHPQQGLDGLTLVDLPDPGDPGPNAIRVRVHATSLNFHDLGVVTGRLPVADGRIP
CCCCCCCCCCCCCCEEEEECCCCCCCCCCEEEEEEEEEECCEECCEEEECCCEECCCCEE
MADGAGVVEAVGSEITEFKPGDHVVSTFFPTWLDGEATLADFATTPGDGIDGYAREVVIA
CCCCCHHHHHHCCHHHHCCCCCHHHHHHCCCCCCCCCEEECCCCCCCCCCCCCCEEEEEE
PATSFTRAPAGWSHAAAATLTTAGVTAWRALVVNGGLKVGDSVLVLGTGGVSIFALQIAR
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHEEEECCCEECCEEEEEECCCHHHHHHHHHH
AMGATVIATSSSDEKLERLRSMGAAHCINYRRDEDWGDRVRALTGGIGVDHVVEVGGPGT
HHCCEEEEECCCHHHHHHHHHCCHHHHHCCCCCCCHHHHHHHHHCCCCCCCEEEECCCHH
LAQSIKAARVSGHISLIGVLTGREGVVPTGMLMARQQRLQGLIVGSRRHQMDLIRGIDAV
HHHHHHHHHHCCCEEEEEEEECCCCCCCHHHHHHHHHHHCEEEECCCHHHHHHHHHHHHH
HGHPVIDRSFPLAAIAKAFRLQATGGHFGKIVLEF
CCCCCCCCCCCHHHHHHHHEEEECCCCEEEEEEEC
>Mature Secondary Structure
MKAIQAHPQQGLDGLTLVDLPDPGDPGPNAIRVRVHATSLNFHDLGVVTGRLPVADGRIP
CCCCCCCCCCCCCCEEEEECCCCCCCCCCEEEEEEEEEECCEECCEEEECCCEECCCCEE
MADGAGVVEAVGSEITEFKPGDHVVSTFFPTWLDGEATLADFATTPGDGIDGYAREVVIA
CCCCCHHHHHHCCHHHHCCCCCHHHHHHCCCCCCCCCEEECCCCCCCCCCCCCCEEEEEE
PATSFTRAPAGWSHAAAATLTTAGVTAWRALVVNGGLKVGDSVLVLGTGGVSIFALQIAR
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHEEEECCCEECCEEEEEECCCHHHHHHHHHH
AMGATVIATSSSDEKLERLRSMGAAHCINYRRDEDWGDRVRALTGGIGVDHVVEVGGPGT
HHCCEEEEECCCHHHHHHHHHCCHHHHHCCCCCCCHHHHHHHHHCCCCCCCEEEECCCHH
LAQSIKAARVSGHISLIGVLTGREGVVPTGMLMARQQRLQGLIVGSRRHQMDLIRGIDAV
HHHHHHHHHHCCCEEEEEEEECCCCCCCHHHHHHHHHHHCEEEECCCHHHHHHHHHHHHH
HGHPVIDRSFPLAAIAKAFRLQATGGHFGKIVLEF
CCCCCCCCCCCHHHHHHHHEEEECCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.017 {9,10-phenanthrenequinone}} 0.0153 {2,6-dichlorophenolindophenol}} 0.013 {2,6-dichlorophenolindophenol}} 0.26 {1,4-naphthoquinone}} 0.065 {1,4-benzoquinone}} 0.007 {NADPH}} 0.0069 {NADPH}} 0.0024 {1,2-naphthoquinone}} [C]

Substrates: NADPH; Quinone [C]

Specific reaction: NADPH + Quinone = NADP(+) + Semiquinone. [C]

General reaction: Oxidation; Reduction [C]

Inhibitor: 2, 3-Dimercaptopropanol; 2, 5-Dichloro-3, 6-dihydroxy -1, 4-benzoquinone; 4-Hydroxy coumarin; 5, 5'-Dithiobis (2-nitrobenzoate); ADP; Cibacronblue3GA; Coumarin; Cu2+; Dicoumarol warfarin, coumarin; Dithiothreitol preincubation with 9, 10-phenanthrenequino

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]