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The map label for this gene is bepF [H]

Identifier: 182679402

GI number: 182679402

Start: 2794293

End: 2795414

Strand: Reverse

Name: bepF [H]

Synonym: Bind_2450

Alternate gene names: 182679402

Gene position: 2795414-2794293 (Counterclockwise)

Preceding gene: 182679407

Following gene: 182679401

Centisome position: 67.03

GC content: 57.13

Gene sequence:

>1122_bases
ATGCGTCTTCCTGTTATCCTGGGGTGTTTCCTCTGTCTTCATTGTGCCGTTCAGGCTCACGCGACAGAGCAAATCATTGC
ACAGTCTTCGGCGCCAACCATCGTCGGCACAACTCTCGTTTCCAAGCAGCCCGTGACCAAGGCTTTGGAGTTTGTCGGCC
GTATTGAGGCTGTCAACAAAGTGGAAGTCCGCGCTCGCGTCACCGGTTACCTAAAGGCGGTCTTGTTCAAGGAAGGCGAC
AAGGTTGTGGAAGGCACACCCTTGTTTCGGATCGAGAAAGAGCCTTTCGACGCCGCGGTCCAGCAGGCTCAGGGTGCTTT
GACCAAGACAAAAGGCATTTTGGCGAATGCCTCGCTCCAGCGTGCTCGTGCGGAGGAATTGCTGGCGAGGCAGGCTGGTA
CCGCCACTGTGCGCGATCAAAGGATCGCGGAAGAGCAAACTGCGCTCGGCGATGTCACGATGACAAAAGCTCAGTTGGAA
TCCGCCGAGATTAATCTTGGCTATACGAATATTACCTCTCCCATCGCGGGCGTTATCGGCCGTACTAATGTGACGAAAGG
CAATGTCGTTGGACCTAACAGCGGGATATTGACAACAATCGTCAGTCTGGATCCAACCTATGTGGTCTTTCCAGTCAGTC
AGCGTGAATTTCTTCGCCTCAAGGATGAAGAACGCAGGGCCGCGGGCGGCGTGCTCAAGGTCGGCCTTCGTTTTTCCGAT
GGCTCGGCCTATGAACAGAGTGGTCGGGTCAATTTCGTCGATGTGACCGTCGACAAATCAACCGATACCGTCACCGTGCG
GGCGAGTGTGCCCAATCCCGGCGGGACGCTCATCGATGGCCAGTTGGTGCGGGTCATGGTGGAAGGCGATAAGCCGCAAG
AAAGAATCACGGTTCCGCAAACCGCGCTGCTTGCCGACCAACAAGGCGCCTACGTCTTTGTCGTTGAAGATGGCAAGGCC
GTGATCCGTCGCCTGAAACTTGGCGGTGAGATTGGATCCGATGTGATCGTCGATAACGGCCTCACGGGCGGCGAGCAAAT
TATCGTCCAGGGGATTGAACGGGTGCGTCCCGGCGGGGCCGTGACAGCCAGCCCGGTCGCGCCGGTCACCGACAGGAGCT
GA

Upstream 100 bases:

>100_bases
GTATTATACTTGTCATTATAGGATGTTGTTTGGTGTCCAAAATTGTGGTGCGCCGACCGCCGGAGAAGCGGATTCATGCG
GCCCAACGGGGATCAATTCG

Downstream 100 bases:

>100_bases
ACCCATGATCTCAGAGGTTTTCGTTCAGCGTCCTCGTCTCGCGACCGTTATCGCGATCGTTACTGTTATTGCCGGCCTTG
TCTCGCTTTTTGTCATCCCC

Product: RND family efflux transporter MFP subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 373; Mature: 373

Protein sequence:

>373_residues
MRLPVILGCFLCLHCAVQAHATEQIIAQSSAPTIVGTTLVSKQPVTKALEFVGRIEAVNKVEVRARVTGYLKAVLFKEGD
KVVEGTPLFRIEKEPFDAAVQQAQGALTKTKGILANASLQRARAEELLARQAGTATVRDQRIAEEQTALGDVTMTKAQLE
SAEINLGYTNITSPIAGVIGRTNVTKGNVVGPNSGILTTIVSLDPTYVVFPVSQREFLRLKDEERRAAGGVLKVGLRFSD
GSAYEQSGRVNFVDVTVDKSTDTVTVRASVPNPGGTLIDGQLVRVMVEGDKPQERITVPQTALLADQQGAYVFVVEDGKA
VIRRLKLGGEIGSDVIVDNGLTGGEQIIVQGIERVRPGGAVTASPVAPVTDRS

Sequences:

>Translated_373_residues
MRLPVILGCFLCLHCAVQAHATEQIIAQSSAPTIVGTTLVSKQPVTKALEFVGRIEAVNKVEVRARVTGYLKAVLFKEGD
KVVEGTPLFRIEKEPFDAAVQQAQGALTKTKGILANASLQRARAEELLARQAGTATVRDQRIAEEQTALGDVTMTKAQLE
SAEINLGYTNITSPIAGVIGRTNVTKGNVVGPNSGILTTIVSLDPTYVVFPVSQREFLRLKDEERRAAGGVLKVGLRFSD
GSAYEQSGRVNFVDVTVDKSTDTVTVRASVPNPGGTLIDGQLVRVMVEGDKPQERITVPQTALLADQQGAYVFVVEDGKA
VIRRLKLGGEIGSDVIVDNGLTGGEQIIVQGIERVRPGGAVTASPVAPVTDRS
>Mature_373_residues
MRLPVILGCFLCLHCAVQAHATEQIIAQSSAPTIVGTTLVSKQPVTKALEFVGRIEAVNKVEVRARVTGYLKAVLFKEGD
KVVEGTPLFRIEKEPFDAAVQQAQGALTKTKGILANASLQRARAEELLARQAGTATVRDQRIAEEQTALGDVTMTKAQLE
SAEINLGYTNITSPIAGVIGRTNVTKGNVVGPNSGILTTIVSLDPTYVVFPVSQREFLRLKDEERRAAGGVLKVGLRFSD
GSAYEQSGRVNFVDVTVDKSTDTVTVRASVPNPGGTLIDGQLVRVMVEGDKPQERITVPQTALLADQQGAYVFVVEDGKA
VIRRLKLGGEIGSDVIVDNGLTGGEQIIVQGIERVRPGGAVTASPVAPVTDRS

Specific function: May contribute to resistance to some drugs, such as deoxycholate, sodium dodecyl sulfate and nalidixic acid, in the absence of BepD and BepE [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789929, Length=355, Percent_Identity=34.0845070422535, Blast_Score=179, Evalue=2e-46,
Organism=Escherichia coli, GI1786668, Length=339, Percent_Identity=37.4631268436578, Blast_Score=173, Evalue=1e-44,
Organism=Escherichia coli, GI1789665, Length=338, Percent_Identity=34.9112426035503, Blast_Score=156, Evalue=2e-39,
Organism=Escherichia coli, GI87082036, Length=327, Percent_Identity=29.9694189602446, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI87081796, Length=340, Percent_Identity=27.0588235294118, Blast_Score=72, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 39678; Mature: 39678

Theoretical pI: Translated: 8.04; Mature: 8.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLPVILGCFLCLHCAVQAHATEQIIAQSSAPTIVGTTLVSKQPVTKALEFVGRIEAVNK
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCC
VEVRARVTGYLKAVLFKEGDKVVEGTPLFRIEKEPFDAAVQQAQGALTKTKGILANASLQ
EEHHHHHHHHHHHHHHHCCCCEECCCCEEEEECCCHHHHHHHHHCCHHHHCCCCCCCHHH
RARAEELLARQAGTATVRDQRIAEEQTALGDVTMTKAQLESAEINLGYTNITSPIAGVIG
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCEEEEHHHHCCCEEEECCCCHHHHHHHHHC
RTNVTKGNVVGPNSGILTTIVSLDPTYVVFPVSQREFLRLKDEERRAAGGVLKVGLRFSD
CCCCCCCCEECCCCCEEEEEEECCCCEEEEECCCHHHHCCCCHHHHHCCCEEEEEEEECC
GSAYEQSGRVNFVDVTVDKSTDTVTVRASVPNPGGTLIDGQLVRVMVEGDKPQERITVPQ
CCCHHHCCCEEEEEEEEECCCCEEEEEEECCCCCCCEECCEEEEEEEECCCCCCEEECCC
TALLADQQGAYVFVVEDGKAVIRRLKLGGEIGSDVIVDNGLTGGEQIIVQGIERVRPGGA
HHEEECCCCCEEEEECCCHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHHHHCCCCC
VTASPVAPVTDRS
EECCCCCCCCCCC
>Mature Secondary Structure
MRLPVILGCFLCLHCAVQAHATEQIIAQSSAPTIVGTTLVSKQPVTKALEFVGRIEAVNK
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCC
VEVRARVTGYLKAVLFKEGDKVVEGTPLFRIEKEPFDAAVQQAQGALTKTKGILANASLQ
EEHHHHHHHHHHHHHHHCCCCEECCCCEEEEECCCHHHHHHHHHCCHHHHCCCCCCCHHH
RARAEELLARQAGTATVRDQRIAEEQTALGDVTMTKAQLESAEINLGYTNITSPIAGVIG
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCEEEEHHHHCCCEEEECCCCHHHHHHHHHC
RTNVTKGNVVGPNSGILTTIVSLDPTYVVFPVSQREFLRLKDEERRAAGGVLKVGLRFSD
CCCCCCCCEECCCCCEEEEEEECCCCEEEEECCCHHHHCCCCHHHHHCCCEEEEEEEECC
GSAYEQSGRVNFVDVTVDKSTDTVTVRASVPNPGGTLIDGQLVRVMVEGDKPQERITVPQ
CCCHHHCCCEEEEEEEEECCCCEEEEEEECCCCCCCEECCEEEEEEEECCCCCCEEECCC
TALLADQQGAYVFVVEDGKAVIRRLKLGGEIGSDVIVDNGLTGGEQIIVQGIERVRPGGA
HHEEECCCCCEEEEECCCHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHHHHCCCCC
VTASPVAPVTDRS
EECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12271122 [H]