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The map label for this gene is birA [H]

Identifier: 182679338

GI number: 182679338

Start: 2724153

End: 2724947

Strand: Reverse

Name: birA [H]

Synonym: Bind_2384

Alternate gene names: 182679338

Gene position: 2724947-2724153 (Counterclockwise)

Preceding gene: 182679339

Following gene: 182679337

Centisome position: 65.34

GC content: 57.61

Gene sequence:

>795_bases
TTGGATGAACAGGCCGTCGCGCGTGCCTTTCGACTGGCCCATTTCGAAAAGCTTGGCTCCACCAATGATGAAGCCCTGGC
ACGGGCCAGGGCCGGCGATCCGGGTCGTCTCTGGATCGTCGCCGATGAACAGTCTGGAGGCCGAGGTCGCCTGGGCCGCA
CTTGGTCCTCGCCTGCCGGCAATCTCTATGCAAGCCTGCTCTTGCTCGATCCGGCACCTCCATTGCATCTTTCGGAACTA
GGATTTGTGGCGGGTGTCGCACTCGCGGAAAGCGTGCGCGCCCTGGTGGATGCTGATCCGCGCCTAGGGATCAAATGGCC
CAATGATCTCTTGTTCGCGGAGGCTAAACTCGGAGGCATCCTTCTGGAATCGACGCCGCTTCCGGGTGGAGGCCGCGCCT
GCGTCATCGGTATTGGTGTCAATTGTCGGTCCCATCCTGACAATACGCCTTATCCGGCGACAGCCTTGGTCAAGATTGGT
GATGCAGCATTGGCTGAAAAACCAATCGGGCCTGAGCATGTGTTTCGGGCGCTATCAGCCAGTTTGGCGCGATGGCTTGA
AGTTTGGGCAGGCGGAAAGGAATTTGGCCAAATCCGCGTCGAATGGCTGAAATATGCAGCGCATATCGGCCGTACGATCC
AAATCGTGCGGCCGAACGGGCGGATCGAGGGAATTTTCAAGTCTATTGACGCACAAGGGCGTTTGATTCTCACTGGACCG
CAGGGCGAAAAAGTGATCGAAGCCGGGGATGTTTTCTTGAATGCGCCGCCTTCTGATGATTCAAAGGCAATGTAG

Upstream 100 bases:

>100_bases
GGAAGCGACCACCGTGGCGGCAAGGTCGCTGTTTTGATCGTGACGCTCATGGCAGCAGAGTCCCTGTCTCCTATCCGAAG
GACTGACTCTGCTGTTTGAC

Downstream 100 bases:

>100_bases
AACGGGATTTTTGGGAATGATTTGGGGTGACAGTCCAGAAGTTCGGTTCGAGCCCCCTTGGTTGAATTCCATTCCTCAAC
TTCTCCCCGAAACGTCTCGT

Product: biotin--acetyl-CoA-carboxylase ligase

Products: NA

Alternate protein names: Biotin--protein ligase [H]

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MDEQAVARAFRLAHFEKLGSTNDEALARARAGDPGRLWIVADEQSGGRGRLGRTWSSPAGNLYASLLLLDPAPPLHLSEL
GFVAGVALAESVRALVDADPRLGIKWPNDLLFAEAKLGGILLESTPLPGGGRACVIGIGVNCRSHPDNTPYPATALVKIG
DAALAEKPIGPEHVFRALSASLARWLEVWAGGKEFGQIRVEWLKYAAHIGRTIQIVRPNGRIEGIFKSIDAQGRLILTGP
QGEKVIEAGDVFLNAPPSDDSKAM

Sequences:

>Translated_264_residues
MDEQAVARAFRLAHFEKLGSTNDEALARARAGDPGRLWIVADEQSGGRGRLGRTWSSPAGNLYASLLLLDPAPPLHLSEL
GFVAGVALAESVRALVDADPRLGIKWPNDLLFAEAKLGGILLESTPLPGGGRACVIGIGVNCRSHPDNTPYPATALVKIG
DAALAEKPIGPEHVFRALSASLARWLEVWAGGKEFGQIRVEWLKYAAHIGRTIQIVRPNGRIEGIFKSIDAQGRLILTGP
QGEKVIEAGDVFLNAPPSDDSKAM
>Mature_264_residues
MDEQAVARAFRLAHFEKLGSTNDEALARARAGDPGRLWIVADEQSGGRGRLGRTWSSPAGNLYASLLLLDPAPPLHLSEL
GFVAGVALAESVRALVDADPRLGIKWPNDLLFAEAKLGGILLESTPLPGGGRACVIGIGVNCRSHPDNTPYPATALVKIG
DAALAEKPIGPEHVFRALSASLARWLEVWAGGKEFGQIRVEWLKYAAHIGRTIQIVRPNGRIEGIFKSIDAQGRLILTGP
QGEKVIEAGDVFLNAPPSDDSKAM

Specific function: Activates biotin to form biotinyl-5'-adenylate and transfers the biotin moiety to biotin-accepting proteins [H]

COG id: COG0340

COG function: function code H; Biotin-(acetyl-CoA carboxylase) ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the biotin--protein ligase family [H]

Homologues:

Organism=Escherichia coli, GI1790408, Length=229, Percent_Identity=34.061135371179, Blast_Score=83, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004408
- InterPro:   IPR003142
- InterPro:   IPR004143 [H]

Pfam domain/function: PF02237 BPL_C; PF03099 BPL_LipA_LipB [H]

EC number: =6.3.4.15 [H]

Molecular weight: Translated: 28131; Mature: 28131

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDEQAVARAFRLAHFEKLGSTNDEALARARAGDPGRLWIVADEQSGGRGRLGRTWSSPAG
CCHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCCCC
NLYASLLLLDPAPPLHLSELGFVAGVALAESVRALVDADPRLGIKWPNDLLFAEAKLGGI
CEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHEEEECCCCCE
LLESTPLPGGGRACVIGIGVNCRSHPDNTPYPATALVKIGDAALAEKPIGPEHVFRALSA
EEECCCCCCCCCEEEEEECCCCCCCCCCCCCCCEEEEEECCHHHHCCCCCHHHHHHHHHH
SLARWLEVWAGGKEFGQIRVEWLKYAAHIGRTIQIVRPNGRIEGIFKSIDAQGRLILTGP
HHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHCCCCCEEEEECC
QGEKVIEAGDVFLNAPPSDDSKAM
CCCEEEEECCEEEECCCCCCCCCC
>Mature Secondary Structure
MDEQAVARAFRLAHFEKLGSTNDEALARARAGDPGRLWIVADEQSGGRGRLGRTWSSPAG
CCHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCCCC
NLYASLLLLDPAPPLHLSELGFVAGVALAESVRALVDADPRLGIKWPNDLLFAEAKLGGI
CEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHEEEECCCCCE
LLESTPLPGGGRACVIGIGVNCRSHPDNTPYPATALVKIGDAALAEKPIGPEHVFRALSA
EEECCCCCCCCCEEEEEECCCCCCCCCCCCCCCEEEEEECCHHHHCCCCCHHHHHHHHHH
SLARWLEVWAGGKEFGQIRVEWLKYAAHIGRTIQIVRPNGRIEGIFKSIDAQGRLILTGP
HHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHCCCCCEEEEECC
QGEKVIEAGDVFLNAPPSDDSKAM
CCCEEEEECCEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8422400 [H]