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The map label for this gene is 182679211

Identifier: 182679211

GI number: 182679211

Start: 2584700

End: 2586082

Strand: Reverse

Name: 182679211

Synonym: Bind_2251

Alternate gene names: NA

Gene position: 2586082-2584700 (Counterclockwise)

Preceding gene: 182679212

Following gene: 182679208

Centisome position: 62.01

GC content: 58.35

Gene sequence:

>1383_bases
ATGGCCCGCGACGTTTCGGATTCAACGCTCATCACGTCGCGCACGGAACTCGTCGCGTGGTTTTTCGATGGAATGAAATC
GCCCAGTCAATTCCGGGTCGGCACCGAACACGAAAAATTCCCCTTTTACCGCTCTAGCCATGCGCCGGTGCCCTATGAGG
GTGAGCGGGGTATCAAGGCCTTGCTCGATGGCATGAAGGAAGCGCTGCAATGGGCGCCGATCACGGATCGAGACAATATT
ATCGGCCTTTATGATGAAAAGGGCGGTGGTGCGATCTCTCTCGAACCCGGCGGCCAGTTCGAGCTGTCTGGCGCTCCGCT
TGAGACCATCCACGAGACAAGCGAGGAATTGACGAGCCATTTCGCGATGCTCGAAAAGGTCGCGGGTCCGCTCGATATCG
GTTTTCTTCTGATCGGCATGACGCCTAAATGGCAGCGCGAAGAGATTCCCGCCATGCCGAAACAGCGCTACGACATCATG
CGCCGTTATATGCCGAAGGTCGGCACGCGGGGCCTCGACATGATGTTTCGGACCTCGACCGTTCAGGCCAATCTCGATTA
TGCGAGCGAGGCCGATATGGTGCTGAAACTGCGCGTTACGCTGGCCCTGCAACCCTTGCTGACGGCGCTTTTCGCCAATT
CTCCTTTCACGGACGGCAAGCTCAACGGCCTGCTTTCCGCGCGGTCGGAGATCTGGCGCGATACGGATCGCGCCCGTTCC
GGCATGCTGCCTTTTGCCTTCGAGGATGGCATGGGTTTCGAGCGTTATGTCGATTATGCGCTCGATGTGCCGATGTATTT
CGTCAAACGCGGTGAAACCTATCACGATGTGGCAGGGGCGAGTTTTCGCGATCTTCTGGCGGGCAAGCTGCCGCAATTGC
CGGGCGAACAGGCGACCTTGTCCGACTGGGCCAATCATTTATCGACGATTTTTCCGGAAGTGCGGCTGAAACGTTATCTT
GAAATGCGCGGTGCCGATGTCGGCCCCATTCCGGCTTGCCTTGCCCTCTCGGCGCTCTGCGTCGGTTTGCTCTATGATCA
GACATCGCTTGATGCCGCCTGGGACCTGGTCAAATCCTGGAACGCCGAGGAACGCCAGGCTCTGCGTGATGATGTGCCGC
GCTTGGGGCTTGAGGCCTCGATCAAGGGCCGCAAATTGCGCGAGATCGGCCGTGAGGTTTTGACCCTGGCTCAGGCCGGA
TTGAAGGCGCGCGACAAGCGTGATGCGGTTGGACGGGACGAGACCTATTTCCTCGCTCCGCTCGACGCTGTCCTGAAAGA
GGGGACGGAAGCGGAAAAGCTGATCGCCCTTTATAAGAAGGATTGGAACGGTTCGGTGGAAAAGCTCTTCGATCAGGGCT
CACCGGTCTTCGCGCCCGTTTGA

Upstream 100 bases:

>100_bases
CACAAATGCTCCCTATCTCTTTGTTTTAACGCATAATACCTGGCCCCAAAGTGTCCAGCTTTCGGGAGGGGTATGCTCTA
AAGTAGATGAAAGCGCGTCA

Downstream 100 bases:

>100_bases
AGGCAATCTTTTGCTCCATGGACCGCAATAAGGCGCCGCAATAAGGCTCGGCATTAAAGCGCGTATTGGTGGATCACCGG
GCGAAAAACGGATCCATGGC

Product: glutamate--cysteine ligase

Products: ADP; phosphate; gamma-L-glutamyl-L-cysteine

Alternate protein names: Glutamate/Cysteine Ligase; Gamma-Glutamylcysteine Synthetase; Glutamate/Cysteine Ligase Family Protein; Glutamate-Cysteine Ligase GshA; Glutamylcysteine Synthetase; Glutamate-Cysteine Ligase Family 2 Protein; Glutamate-Cysteine Ligase Protein; Glutathione Synthetase; Glutamate-Cysteine Ligase Gsha; Glutamate-Cysteine Ligase Protein; Glutamate-Cysteine Ligase Family Protein; Glutamate Cystein Ligase Protein; Glutamate-Cysteine Ligase GSHA

Number of amino acids: Translated: 460; Mature: 459

Protein sequence:

>460_residues
MARDVSDSTLITSRTELVAWFFDGMKSPSQFRVGTEHEKFPFYRSSHAPVPYEGERGIKALLDGMKEALQWAPITDRDNI
IGLYDEKGGGAISLEPGGQFELSGAPLETIHETSEELTSHFAMLEKVAGPLDIGFLLIGMTPKWQREEIPAMPKQRYDIM
RRYMPKVGTRGLDMMFRTSTVQANLDYASEADMVLKLRVTLALQPLLTALFANSPFTDGKLNGLLSARSEIWRDTDRARS
GMLPFAFEDGMGFERYVDYALDVPMYFVKRGETYHDVAGASFRDLLAGKLPQLPGEQATLSDWANHLSTIFPEVRLKRYL
EMRGADVGPIPACLALSALCVGLLYDQTSLDAAWDLVKSWNAEERQALRDDVPRLGLEASIKGRKLREIGREVLTLAQAG
LKARDKRDAVGRDETYFLAPLDAVLKEGTEAEKLIALYKKDWNGSVEKLFDQGSPVFAPV

Sequences:

>Translated_460_residues
MARDVSDSTLITSRTELVAWFFDGMKSPSQFRVGTEHEKFPFYRSSHAPVPYEGERGIKALLDGMKEALQWAPITDRDNI
IGLYDEKGGGAISLEPGGQFELSGAPLETIHETSEELTSHFAMLEKVAGPLDIGFLLIGMTPKWQREEIPAMPKQRYDIM
RRYMPKVGTRGLDMMFRTSTVQANLDYASEADMVLKLRVTLALQPLLTALFANSPFTDGKLNGLLSARSEIWRDTDRARS
GMLPFAFEDGMGFERYVDYALDVPMYFVKRGETYHDVAGASFRDLLAGKLPQLPGEQATLSDWANHLSTIFPEVRLKRYL
EMRGADVGPIPACLALSALCVGLLYDQTSLDAAWDLVKSWNAEERQALRDDVPRLGLEASIKGRKLREIGREVLTLAQAG
LKARDKRDAVGRDETYFLAPLDAVLKEGTEAEKLIALYKKDWNGSVEKLFDQGSPVFAPV
>Mature_459_residues
ARDVSDSTLITSRTELVAWFFDGMKSPSQFRVGTEHEKFPFYRSSHAPVPYEGERGIKALLDGMKEALQWAPITDRDNII
GLYDEKGGGAISLEPGGQFELSGAPLETIHETSEELTSHFAMLEKVAGPLDIGFLLIGMTPKWQREEIPAMPKQRYDIMR
RYMPKVGTRGLDMMFRTSTVQANLDYASEADMVLKLRVTLALQPLLTALFANSPFTDGKLNGLLSARSEIWRDTDRARSG
MLPFAFEDGMGFERYVDYALDVPMYFVKRGETYHDVAGASFRDLLAGKLPQLPGEQATLSDWANHLSTIFPEVRLKRYLE
MRGADVGPIPACLALSALCVGLLYDQTSLDAAWDLVKSWNAEERQALRDDVPRLGLEASIKGRKLREIGREVLTLAQAGL
KARDKRDAVGRDETYFLAPLDAVLKEGTEAEKLIALYKKDWNGSVEKLFDQGSPVFAPV

Specific function: Unknown

COG id: COG3572

COG function: function code H; Gamma-glutamylcysteine synthetase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 6.3.2.2

Molecular weight: Translated: 51355; Mature: 51224

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARDVSDSTLITSRTELVAWFFDGMKSPSQFRVGTEHEKFPFYRSSHAPVPYEGERGIKA
CCCCCCCCCEEHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHH
LLDGMKEALQWAPITDRDNIIGLYDEKGGGAISLEPGGQFELSGAPLETIHETSEELTSH
HHHHHHHHHHCCCCCCCCCEEEEEECCCCCEEEECCCCCEEECCCCHHHHHHHHHHHHHH
FAMLEKVAGPLDIGFLLIGMTPKWQREEIPAMPKQRYDIMRRYMPKVGTRGLDMMFRTST
HHHHHHHCCCCCEEEEEEECCCCCCHHCCCCCCHHHHHHHHHHHHHHCCCCHHHEEEECE
VQANLDYASEADMVLKLRVTLALQPLLTALFANSPFTDGKLNGLLSARSEIWRDTDRARS
EEECCCCCCCCCEEEEHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHC
GMLPFAFEDGMGFERYVDYALDVPMYFVKRGETYHDVAGASFRDLLAGKLPQLPGEQATL
CCCCEEECCCCCHHHHHHHHHCCHHHHHHCCCCHHHHCCCHHHHHHHHCCCCCCCCCCHH
SDWANHLSTIFPEVRLKRYLEMRGADVGPIPACLALSALCVGLLYDQTSLDAAWDLVKSW
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCC
NAEERQALRDDVPRLGLEASIKGRKLREIGREVLTLAQAGLKARDKRDAVGRDETYFLAP
CHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCHHCCHHHCCCCCCEEEECC
LDAVLKEGTEAEKLIALYKKDWNGSVEKLFDQGSPVFAPV
HHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
ARDVSDSTLITSRTELVAWFFDGMKSPSQFRVGTEHEKFPFYRSSHAPVPYEGERGIKA
CCCCCCCCEEHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHH
LLDGMKEALQWAPITDRDNIIGLYDEKGGGAISLEPGGQFELSGAPLETIHETSEELTSH
HHHHHHHHHHCCCCCCCCCEEEEEECCCCCEEEECCCCCEEECCCCHHHHHHHHHHHHHH
FAMLEKVAGPLDIGFLLIGMTPKWQREEIPAMPKQRYDIMRRYMPKVGTRGLDMMFRTST
HHHHHHHCCCCCEEEEEEECCCCCCHHCCCCCCHHHHHHHHHHHHHHCCCCHHHEEEECE
VQANLDYASEADMVLKLRVTLALQPLLTALFANSPFTDGKLNGLLSARSEIWRDTDRARS
EEECCCCCCCCCEEEEHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHC
GMLPFAFEDGMGFERYVDYALDVPMYFVKRGETYHDVAGASFRDLLAGKLPQLPGEQATL
CCCCEEECCCCCHHHHHHHHHCCHHHHHHCCCCHHHHCCCHHHHHHHHCCCCCCCCCCHH
SDWANHLSTIFPEVRLKRYLEMRGADVGPIPACLALSALCVGLLYDQTSLDAAWDLVKSW
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCC
NAEERQALRDDVPRLGLEASIKGRKLREIGREVLTLAQAGLKARDKRDAVGRDETYFLAP
CHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCHHCCHHHCCCCCCEEEECC
LDAVLKEGTEAEKLIALYKKDWNGSVEKLFDQGSPVFAPV
HHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamate; L-cysteine

Specific reaction: ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA