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The map label for this gene is ada [H]

Identifier: 182679081

GI number: 182679081

Start: 2417681

End: 2418781

Strand: Reverse

Name: ada [H]

Synonym: Bind_2118

Alternate gene names: 182679081

Gene position: 2418781-2417681 (Counterclockwise)

Preceding gene: 182679085

Following gene: 182679080

Centisome position: 58.0

GC content: 59.04

Gene sequence:

>1101_bases
ATGCGTCAGCATGCTCTTCCAAGGGGTACACAGCCCGCCGTCCCGATCGAGGATGATCCGCGTTGGATAGCCGTTGTCGA
TCGCGATTCTCGTTTTGACGGCACCTTCGTCTATTCCGTGAAAACGACAGGTATTTATTGCCGGCCGTCCTGCCCCTCAC
GTCTGGCGAAGCCAGGCAATATCCGTTTTCATGCTCATTGCGCGGCCGCGGAAAAAGCAGGGTTTCGGCCGTGCCTCAGG
TGCCGTCCACATGAGGCGTCGCTCGCGCACGATCATGCCGATCTGATCGTTGCGGCCTGCCGCCGGATTGAAACGGCGCA
GAAGCAACTGAGCCTGGATCAATTGGCGAAGGCCGCCGGCCTGAGCCCGTTTCATTTTCACCGATTGTTCAAATCGATCA
CCGGCCTGAGCCCGAAAGCCTATGGCGCTGCCCATCGCATGAACAAGATCCACAAAGCGCTCAGTGCGGGTGAGGAAAGT
GTGACGGCAACGATCTACGCCTCTGGATATCAGTCATCGAGCCGTTTCTATGCCACCTCAAAGGAGATGTTGGGGATGAC
CGCGACTGCATTCCGAGAAGGAGGCAACTTGGCCGAAATCCGCTTCGCGCTAGGTGAAACCTCGCTCGGCTCCCTTCTTG
TGGCCTGTAGCGCGCAAGGCGTTTGTGCCATTTTCCTTGGCGATGATCCGGAGGCGCTCGTCCATGAATTGCAGGGGCGG
TTTCCGAAAGCGCATCTCATCGGCGGTGACGAAGGTTTCGAGGCTCTGGTCGCCAAGGTCGTCGGTTTCGTCGAAACGCC
CGCACGCGGCCTCGATCTGCCGCTGGATATTCGTGGTACGGCGTTTCAGCACAAGGTCTGGCAGGCCTTGTGTGAAGTTC
CTTTCGGAGAGACTGCCAGCTACACTGACATCGCCCGGCGGATTGGCGCGCCCAAGGCCGTCCGCGCCGTGGCGCAAGCC
TGCGCTGCTAACAAGATCGCCGTGGCTATCCCCTGTCATCGTATTTTGCGTACTGACGGAAGCTTGTCTGGCTATCGCTG
GGGCGTCGAGCGCAAGCGCGCCTTGCTTTTGAAGGAGGGCGCTACGAAGAATTCGAACTGA

Upstream 100 bases:

>100_bases
GCGTCTTTGGCGTGATTTCGTCGATTCGACAGCAAGAATCGGAGTGCGCCGCAATCTTGTCCTCGCTATGGAGAATGATC
TGGAAAAGAGGAGCAGGATC

Downstream 100 bases:

>100_bases
AGGGCTTCGTTAGGATACCATCACTTTGAATAAGCGATTGATACGAGAGTATTCAATTTACGGTCCTCTGATCAGAACCA
ATTTGAGCGAAATCGACAAC

Product: AraC family transcriptional regulator

Products: NA

Alternate protein names: Regulatory protein of adaptative response; O-6-methylguanine-DNA alkyltransferase [H]

Number of amino acids: Translated: 366; Mature: 366

Protein sequence:

>366_residues
MRQHALPRGTQPAVPIEDDPRWIAVVDRDSRFDGTFVYSVKTTGIYCRPSCPSRLAKPGNIRFHAHCAAAEKAGFRPCLR
CRPHEASLAHDHADLIVAACRRIETAQKQLSLDQLAKAAGLSPFHFHRLFKSITGLSPKAYGAAHRMNKIHKALSAGEES
VTATIYASGYQSSSRFYATSKEMLGMTATAFREGGNLAEIRFALGETSLGSLLVACSAQGVCAIFLGDDPEALVHELQGR
FPKAHLIGGDEGFEALVAKVVGFVETPARGLDLPLDIRGTAFQHKVWQALCEVPFGETASYTDIARRIGAPKAVRAVAQA
CAANKIAVAIPCHRILRTDGSLSGYRWGVERKRALLLKEGATKNSN

Sequences:

>Translated_366_residues
MRQHALPRGTQPAVPIEDDPRWIAVVDRDSRFDGTFVYSVKTTGIYCRPSCPSRLAKPGNIRFHAHCAAAEKAGFRPCLR
CRPHEASLAHDHADLIVAACRRIETAQKQLSLDQLAKAAGLSPFHFHRLFKSITGLSPKAYGAAHRMNKIHKALSAGEES
VTATIYASGYQSSSRFYATSKEMLGMTATAFREGGNLAEIRFALGETSLGSLLVACSAQGVCAIFLGDDPEALVHELQGR
FPKAHLIGGDEGFEALVAKVVGFVETPARGLDLPLDIRGTAFQHKVWQALCEVPFGETASYTDIARRIGAPKAVRAVAQA
CAANKIAVAIPCHRILRTDGSLSGYRWGVERKRALLLKEGATKNSN
>Mature_366_residues
MRQHALPRGTQPAVPIEDDPRWIAVVDRDSRFDGTFVYSVKTTGIYCRPSCPSRLAKPGNIRFHAHCAAAEKAGFRPCLR
CRPHEASLAHDHADLIVAACRRIETAQKQLSLDQLAKAAGLSPFHFHRLFKSITGLSPKAYGAAHRMNKIHKALSAGEES
VTATIYASGYQSSSRFYATSKEMLGMTATAFREGGNLAEIRFALGETSLGSLLVACSAQGVCAIFLGDDPEALVHELQGR
FPKAHLIGGDEGFEALVAKVVGFVETPARGLDLPLDIRGTAFQHKVWQALCEVPFGETASYTDIARRIGAPKAVRAVAQA
CAANKIAVAIPCHRILRTDGSLSGYRWGVERKRALLLKEGATKNSN

Specific function: The methylated ADA protein acts as a positive regulator of its own synthesis, as well as that of other proteins. The transcription-activating function of the ADA protein resides in its N-terminus. It activates the transcription of alkA, alkB and aidB [H]

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=169, Percent_Identity=28.9940828402367, Blast_Score=65, Evalue=1e-10,
Organism=Escherichia coli, GI1788542, Length=351, Percent_Identity=49.5726495726496, Blast_Score=345, Evalue=2e-96,
Organism=Escherichia coli, GI1787596, Length=164, Percent_Identity=33.5365853658537, Blast_Score=85, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI115533068, Length=173, Percent_Identity=40.4624277456647, Blast_Score=109, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI115533070, Length=82, Percent_Identity=58.5365853658537, Blast_Score=106, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6320001, Length=81, Percent_Identity=46.9135802469136, Blast_Score=91, Evalue=4e-19,
Organism=Drosophila melanogaster, GI17137554, Length=175, Percent_Identity=34.8571428571429, Blast_Score=88, Evalue=9e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004026
- InterPro:   IPR016221
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR018060
- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF02805 Ada_Zn_binding; PF01035 DNA_binding_1; PF00165 HTH_AraC; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 39617; Mature: 39617

Theoretical pI: Translated: 9.45; Mature: 9.45

Prosite motif: PS01124 HTH_ARAC_FAMILY_2 ; PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRQHALPRGTQPAVPIEDDPRWIAVVDRDSRFDGTFVYSVKTTGIYCRPSCPSRLAKPGN
CCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCEEEEEEEECCEEECCCCHHHHCCCCC
IRFHAHCAAAEKAGFRPCLRCRPHEASLAHDHADLIVAACRRIETAQKQLSLDQLAKAAG
EEEEEEECHHHHCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHC
LSPFHFHRLFKSITGLSPKAYGAAHRMNKIHKALSAGEESVTATIYASGYQSSSRFYATS
CCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCEEEECH
KEMLGMTATAFREGGNLAEIRFALGETSLGSLLVACSAQGVCAIFLGDDPEALVHELQGR
HHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHEECCCCEEEEEECCCHHHHHHHHHCC
FPKAHLIGGDEGFEALVAKVVGFVETPARGLDLPLDIRGTAFQHKVWQALCEVPFGETAS
CCCEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCEECCCCHHHHHHHHHHHCCCCCCCCC
YTDIARRIGAPKAVRAVAQACAANKIAVAIPCHRILRTDGSLSGYRWGVERKRALLLKEG
HHHHHHHHCCCHHHHHHHHHHCCCCEEEEECHHHHHHCCCCCCCEECCHHHHHHEEEECC
ATKNSN
CCCCCC
>Mature Secondary Structure
MRQHALPRGTQPAVPIEDDPRWIAVVDRDSRFDGTFVYSVKTTGIYCRPSCPSRLAKPGN
CCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCEEEEEEEECCEEECCCCHHHHCCCCC
IRFHAHCAAAEKAGFRPCLRCRPHEASLAHDHADLIVAACRRIETAQKQLSLDQLAKAAG
EEEEEEECHHHHCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHC
LSPFHFHRLFKSITGLSPKAYGAAHRMNKIHKALSAGEESVTATIYASGYQSSSRFYATS
CCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCEEEECH
KEMLGMTATAFREGGNLAEIRFALGETSLGSLLVACSAQGVCAIFLGDDPEALVHELQGR
HHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHEECCCCEEEEEECCCHHHHHHHHHCC
FPKAHLIGGDEGFEALVAKVVGFVETPARGLDLPLDIRGTAFQHKVWQALCEVPFGETAS
CCCEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCEECCCCHHHHHHHHHHHCCCCCCCCC
YTDIARRIGAPKAVRAVAQACAANKIAVAIPCHRILRTDGSLSGYRWGVERKRALLLKEG
HHHHHHHHCCCHHHHHHHHHHCCCCEEEEECHHHHHHCCCCCCCEECCHHHHHHEEEECC
ATKNSN
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2987251; 3887409; 9097040; 9278503; 2982792; 3536913; 3009022; 3529081; 1581309; 8202360; 8500619; 8156986 [H]