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The map label for this gene is btuB [H]

Identifier: 182679056

GI number: 182679056

Start: 2392257

End: 2394269

Strand: Direct

Name: btuB [H]

Synonym: Bind_2092

Alternate gene names: 182679056

Gene position: 2392257-2394269 (Clockwise)

Preceding gene: 182679055

Following gene: 182679057

Centisome position: 57.37

GC content: 57.63

Gene sequence:

>2013_bases
GTGCTCTCCCCATCCTTGCGCATGGCGCAAAGCGGATTCTTTCCGCGCGCTTTATACCATTCGTGTTTTCTTTCCTGTCT
CTTGACGTCCTCCGCATTCAGCGAGGAAGCCTTGCCCGCTCTCATTGTCACGGCTGACCGTGTCGAGGAGCCAATTTCCC
GCACCGCATCCTCGATAACGATCATCCCTTCGGAGGAAATAGAGAAACGGGGCACTTTCGGCCTGCCGAACGTATTGCGC
GGCGTTCCAGGGCTTGATCTTTACGAAACTGGCGGTCCCGGCACCGCCACTTCGGTTTTTCTGCGTGGTTCGGCCCCTGG
ACAAACCTTGGTGCTGATCGATGGCATGCGTATCGGCGATCCCTCCTCGATCGATGGCTCTGTCGATCTCGGTGGCCTCG
TCGCCACGGATCTCGACCAGATCGAGGTGCTGCGTGGTCCGCAATCGGCCCTCTATGGTTCGGCTGCCATGGGCGGCGTC
ATCAATATCGTCACACGCAAAGGCAGCGGCGCGCCACGCGCCTCAGTCCTCATGGAAGGGGGCAGATATGGCACGGCGCA
CACACGCGCAGCCCTGTCCGGCGGCACTGACCGCCTCTCCTACGCATTCTCCATTGATGGCCTTTATACGTCTGCCTTTC
CACGCTACGGCTATCGCATCACGAGGCCTCTGACGATCGGCGATGGCGTGACACCCCTACCACCAGCGCCGTCAAGCGAC
CCAACCCGCAAAGGAGGCTTCCACGGTCGCCTCGCCTATAACTTTACGAACGATATTTCAATCGAGGGCGGTGTGATCGG
CTATGATAGTTCAATCCGCTTCGACAATCCTTACGCATACAATACTGCTGACATCTTCAATCCCTATAATCATCAGCATG
CAACCTTCCTGCAAGGTTATCTCCTGGGCAAGGCCGACCTGTTCGACCATTTTCTGCACAACCGCCTGAGCCTTTTCGGC
AATATCACCGACCACTCGGTCTGGCAGGCCCAATCCTGCTATGACACCGCCTATAACGCATTCGACTGCCGCATGGGTTT
TCGCGGCGCGCGGCGCGGCGGCGAATATCAGGGTGATGCGGTCCTCGGCTCCTTGGGCCTTTTCACTTTCGGGGTGCGTC
TTGAGAAAGAAGTCGCGGACACGTCACAGGACCCAGCGCCGCCCGGTGCTTTTGATCCGATCCATGCGAGCCAGACCACT
TATTCCGGCTTTGCACAACATCAGCTCACCCTGTTCGACCGGTTCGATATCACCTATGGCGGGCGCATGGACGCCATTGA
CAAAGGCCCAACCTTTACAACCTGGAGAGCAACCGGTGCCTATCGGATCGAGGAGACGGGAACCAAGCTGCGCGCAAGCG
CAGGCACTGGCGCGCGTATCGGCTCGCTCTATCAAAGGTTTAGCCAATACGGCAATCCCGCGCTCGCACCCGAATATAGC
ATCGGCTATGATGCAGGTATCGAGCAAACCCTGTTCGGCGGACATGTCTCGTTCTCAGCCAATTATTTCGAAAACAGATT
TCGCAATCTGATCGATTTCGGCATGTCGGCGAGCTGCTTCGCGATTCAGGTCTCCGGCTGTTACAATAATATCGGGCGAG
CCGAGACGAAAGGCGTCGAATTCGCCAGCGAAGCCGTCCTTATTCCCGACGAATGGCGCCTCCGCGCGAGCTATACGCAT
CTCGTCGCGACGAATTTAGACACACAGCAGCGCGTGCCACGGCGCCCTCGCGACAAAGGCATGCTGTCGATCATCTATAG
CGCCATTCCGGATTTCGAGTTCGAGGCACGCGGCCTTTTTGTCAGCGCGACGCCCGATTACGACTATACATACGGCCACG
CCGTCATGCTCCCGGCCTATGCACGGATCGACCTGTTCGCCAGCTACAAACTGAATGAAACCGTGACCTGTTTCGCGCGC
ATAGAGAATGTCAATGATGCACGCTACGAGGAAGTCTATAATTACGGAACGCCAGGGCGGTCCCTCTACGGAGGCCTCAG
AGTGACATGGTAG

Upstream 100 bases:

>100_bases
TGCACCGGTGCGGTCGAACTTATTCTATGTGCGTTCAGCACCTCTAGCTAGGTGAAGACCCTTCGCAGTGACGGCCCACG
TTTGCTGCGAGTCTCTCCGC

Downstream 100 bases:

>100_bases
GAAATGGCCGATGACGACAACAGCAACACCCCCTTCCAAACAGACACAGACGGTGCCGCAGCGCGTGATCGGCGCTGATG
GTGTCCCGCTCGATGTCTTT

Product: TonB-dependent receptor

Products: NA

Alternate protein names: Cobalamin receptor; Outer membrane cobalamin translocator [H]

Number of amino acids: Translated: 670; Mature: 670

Protein sequence:

>670_residues
MLSPSLRMAQSGFFPRALYHSCFLSCLLTSSAFSEEALPALIVTADRVEEPISRTASSITIIPSEEIEKRGTFGLPNVLR
GVPGLDLYETGGPGTATSVFLRGSAPGQTLVLIDGMRIGDPSSIDGSVDLGGLVATDLDQIEVLRGPQSALYGSAAMGGV
INIVTRKGSGAPRASVLMEGGRYGTAHTRAALSGGTDRLSYAFSIDGLYTSAFPRYGYRITRPLTIGDGVTPLPPAPSSD
PTRKGGFHGRLAYNFTNDISIEGGVIGYDSSIRFDNPYAYNTADIFNPYNHQHATFLQGYLLGKADLFDHFLHNRLSLFG
NITDHSVWQAQSCYDTAYNAFDCRMGFRGARRGGEYQGDAVLGSLGLFTFGVRLEKEVADTSQDPAPPGAFDPIHASQTT
YSGFAQHQLTLFDRFDITYGGRMDAIDKGPTFTTWRATGAYRIEETGTKLRASAGTGARIGSLYQRFSQYGNPALAPEYS
IGYDAGIEQTLFGGHVSFSANYFENRFRNLIDFGMSASCFAIQVSGCYNNIGRAETKGVEFASEAVLIPDEWRLRASYTH
LVATNLDTQQRVPRRPRDKGMLSIIYSAIPDFEFEARGLFVSATPDYDYTYGHAVMLPAYARIDLFASYKLNETVTCFAR
IENVNDARYEEVYNYGTPGRSLYGGLRVTW

Sequences:

>Translated_670_residues
MLSPSLRMAQSGFFPRALYHSCFLSCLLTSSAFSEEALPALIVTADRVEEPISRTASSITIIPSEEIEKRGTFGLPNVLR
GVPGLDLYETGGPGTATSVFLRGSAPGQTLVLIDGMRIGDPSSIDGSVDLGGLVATDLDQIEVLRGPQSALYGSAAMGGV
INIVTRKGSGAPRASVLMEGGRYGTAHTRAALSGGTDRLSYAFSIDGLYTSAFPRYGYRITRPLTIGDGVTPLPPAPSSD
PTRKGGFHGRLAYNFTNDISIEGGVIGYDSSIRFDNPYAYNTADIFNPYNHQHATFLQGYLLGKADLFDHFLHNRLSLFG
NITDHSVWQAQSCYDTAYNAFDCRMGFRGARRGGEYQGDAVLGSLGLFTFGVRLEKEVADTSQDPAPPGAFDPIHASQTT
YSGFAQHQLTLFDRFDITYGGRMDAIDKGPTFTTWRATGAYRIEETGTKLRASAGTGARIGSLYQRFSQYGNPALAPEYS
IGYDAGIEQTLFGGHVSFSANYFENRFRNLIDFGMSASCFAIQVSGCYNNIGRAETKGVEFASEAVLIPDEWRLRASYTH
LVATNLDTQQRVPRRPRDKGMLSIIYSAIPDFEFEARGLFVSATPDYDYTYGHAVMLPAYARIDLFASYKLNETVTCFAR
IENVNDARYEEVYNYGTPGRSLYGGLRVTW
>Mature_670_residues
MLSPSLRMAQSGFFPRALYHSCFLSCLLTSSAFSEEALPALIVTADRVEEPISRTASSITIIPSEEIEKRGTFGLPNVLR
GVPGLDLYETGGPGTATSVFLRGSAPGQTLVLIDGMRIGDPSSIDGSVDLGGLVATDLDQIEVLRGPQSALYGSAAMGGV
INIVTRKGSGAPRASVLMEGGRYGTAHTRAALSGGTDRLSYAFSIDGLYTSAFPRYGYRITRPLTIGDGVTPLPPAPSSD
PTRKGGFHGRLAYNFTNDISIEGGVIGYDSSIRFDNPYAYNTADIFNPYNHQHATFLQGYLLGKADLFDHFLHNRLSLFG
NITDHSVWQAQSCYDTAYNAFDCRMGFRGARRGGEYQGDAVLGSLGLFTFGVRLEKEVADTSQDPAPPGAFDPIHASQTT
YSGFAQHQLTLFDRFDITYGGRMDAIDKGPTFTTWRATGAYRIEETGTKLRASAGTGARIGSLYQRFSQYGNPALAPEYS
IGYDAGIEQTLFGGHVSFSANYFENRFRNLIDFGMSASCFAIQVSGCYNNIGRAETKGVEFASEAVLIPDEWRLRASYTH
LVATNLDTQQRVPRRPRDKGMLSIIYSAIPDFEFEARGLFVSATPDYDYTYGHAVMLPAYARIDLFASYKLNETVTCFAR
IENVNDARYEEVYNYGTPGRSLYGGLRVTW

Specific function: Involved in the active translocation of vitamin B12 (cyanocobalamin) across the outer membrane to the periplasmic space. It derives its energy for transport by interacting with the trans-periplasmic membrane protein TonB [H]

COG id: COG4206

COG function: function code H; Outer membrane cobalamin receptor protein

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TonB-dependent receptor family. BtuB (TC 1.B.14.3.1) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790405, Length=648, Percent_Identity=25.3086419753086, Blast_Score=151, Evalue=1e-37,
Organism=Escherichia coli, GI1788478, Length=146, Percent_Identity=34.2465753424658, Blast_Score=94, Evalue=3e-20,
Organism=Escherichia coli, GI1786798, Length=147, Percent_Identity=33.3333333333333, Blast_Score=72, Evalue=1e-13,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012910
- InterPro:   IPR000531
- InterPro:   IPR010101
- InterPro:   IPR010916 [H]

Pfam domain/function: PF07715 Plug; PF00593 TonB_dep_Rec [H]

EC number: NA

Molecular weight: Translated: 73104; Mature: 73104

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSPSLRMAQSGFFPRALYHSCFLSCLLTSSAFSEEALPALIVTADRVEEPISRTASSIT
CCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHCCCEEE
IIPSEEIEKRGTFGLPNVLRGVPGLDLYETGGPGTATSVFLRGSAPGQTLVLIDGMRIGD
EEECHHHHHCCCCCCCHHHHCCCCCCEEECCCCCCEEEEEEECCCCCCEEEEEECEECCC
PSSIDGSVDLGGLVATDLDQIEVLRGPQSALYGSAAMGGVINIVTRKGSGAPRASVLMEG
CCCCCCCCCCCCEEECCHHHHHHHCCCHHHHHCCHHHCCEEEEEEECCCCCCCHHEEEEC
GRYGTAHTRAALSGGTDRLSYAFSIDGLYTSAFPRYGYRITRPLTIGDGVTPLPPAPSSD
CCCCCHHHHHHHCCCCCCEEEEEEECCHHHCCCCCCCEEEECEEEECCCCCCCCCCCCCC
PTRKGGFHGRLAYNFTNDISIEGGVIGYDSSIRFDNPYAYNTADIFNPYNHQHATFLQGY
CCCCCCCCCEEEEECCCCEEECCCEEECCCCEEECCCCCCCCHHHCCCCCCCHHHHHHHH
LLGKADLFDHFLHNRLSLFGNITDHSVWQAQSCYDTAYNAFDCRMGFRGARRGGEYQGDA
HCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCEEECCCCCHHCCCCCCCCCH
VLGSLGLFTFGVRLEKEVADTSQDPAPPGAFDPIHASQTTYSGFAQHQLTLFDRFDITYG
HHHHHHHHHHCEEEEHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHEEEEEEEEECEEEC
GRMDAIDKGPTFTTWRATGAYRIEETGTKLRASAGTGARIGSLYQRFSQYGNPALAPEYS
CCCCCCCCCCCEEEEECCCEEEEECCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCC
IGYDAGIEQTLFGGHVSFSANYFENRFRNLIDFGMSASCFAIQVSGCYNNIGRAETKGVE
CCCCCCCCHHEECCEEEEEHHHHHHHHHHHHHCCCCCEEEEEEEECHHCCCCCCCCCCCC
FASEAVLIPDEWRLRASYTHLVATNLDTQQRVPRRPRDKGMLSIIYSAIPDFEFEARGLF
CCCCEEECCCCCEEEEEEEEEEEECCCHHHHCCCCCCCCHHHHHHHHHCCCCCEECCEEE
VSATPDYDYTYGHAVMLPAYARIDLFASYKLNETVTCFARIENVNDARYEEVYNYGTPGR
EECCCCCCCCCCCEEEECCEEEEEEEEEECCCCEEEEEEEECCCCCHHHHHHHCCCCCCH
SLYGGLRVTW
HHHCCEEEEC
>Mature Secondary Structure
MLSPSLRMAQSGFFPRALYHSCFLSCLLTSSAFSEEALPALIVTADRVEEPISRTASSIT
CCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHCCCEEE
IIPSEEIEKRGTFGLPNVLRGVPGLDLYETGGPGTATSVFLRGSAPGQTLVLIDGMRIGD
EEECHHHHHCCCCCCCHHHHCCCCCCEEECCCCCCEEEEEEECCCCCCEEEEEECEECCC
PSSIDGSVDLGGLVATDLDQIEVLRGPQSALYGSAAMGGVINIVTRKGSGAPRASVLMEG
CCCCCCCCCCCCEEECCHHHHHHHCCCHHHHHCCHHHCCEEEEEEECCCCCCCHHEEEEC
GRYGTAHTRAALSGGTDRLSYAFSIDGLYTSAFPRYGYRITRPLTIGDGVTPLPPAPSSD
CCCCCHHHHHHHCCCCCCEEEEEEECCHHHCCCCCCCEEEECEEEECCCCCCCCCCCCCC
PTRKGGFHGRLAYNFTNDISIEGGVIGYDSSIRFDNPYAYNTADIFNPYNHQHATFLQGY
CCCCCCCCCEEEEECCCCEEECCCEEECCCCEEECCCCCCCCHHHCCCCCCCHHHHHHHH
LLGKADLFDHFLHNRLSLFGNITDHSVWQAQSCYDTAYNAFDCRMGFRGARRGGEYQGDA
HCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCEEECCCCCHHCCCCCCCCCH
VLGSLGLFTFGVRLEKEVADTSQDPAPPGAFDPIHASQTTYSGFAQHQLTLFDRFDITYG
HHHHHHHHHHCEEEEHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHEEEEEEEEECEEEC
GRMDAIDKGPTFTTWRATGAYRIEETGTKLRASAGTGARIGSLYQRFSQYGNPALAPEYS
CCCCCCCCCCCEEEEECCCEEEEECCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCC
IGYDAGIEQTLFGGHVSFSANYFENRFRNLIDFGMSASCFAIQVSGCYNNIGRAETKGVE
CCCCCCCCHHEECCEEEEEHHHHHHHHHHHHHCCCCCEEEEEEEECHHCCCCCCCCCCCC
FASEAVLIPDEWRLRASYTHLVATNLDTQQRVPRRPRDKGMLSIIYSAIPDFEFEARGLF
CCCCEEECCCCCEEEEEEEEEEEECCCHHHHCCCCCCCCHHHHHHHHHCCCCCEECCEEE
VSATPDYDYTYGHAVMLPAYARIDLFASYKLNETVTCFARIENVNDARYEEVYNYGTPGR
EECCCCCCCCCCCEEEECCEEEEEEEEEECCCCEEEEEEEECCCCCHHHHHHHCCCCCCH
SLYGGLRVTW
HHHCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 14528314 [H]