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The map label for this gene is rsmB [H]

Identifier: 182679035

GI number: 182679035

Start: 2368584

End: 2369975

Strand: Reverse

Name: rsmB [H]

Synonym: Bind_2071

Alternate gene names: 182679035

Gene position: 2369975-2368584 (Counterclockwise)

Preceding gene: 182679046

Following gene: 182679034

Centisome position: 56.83

GC content: 61.85

Gene sequence:

>1392_bases
GTGGCAAATTCATCCATTCGGCCGGCGGCGTCCGGGCGCTCGAAACTCCGCAAGCCAGCCGCTTCGAGCACCGAGGAGCA
GCCTGCGGGGCTCGGCGTACGGCGGACAGCAGCGGCGATCTTACGGGCGATTGTGACCAAGGGACAGGCGCTCGACGATT
TTTTCGCGCCCGAGTCGAACCCTGAAATTCTGGCGGGTCTCGAGCCGCGTGACAAGGCGCTGGCGCGATCGATCGTCACC
GTGGCTCTAAGAAGGCTTGGCACGATCCGGGCGGCGCTCACCGGGCTTTTGGCACAAGGTCTCCCGCGCCAGGCGCCGGA
CCTCGAATGGATTTTGCTCGTCGCTGGAGCGCAAATCCTGTTTCTTGACGTTCCCGATCATGCCGCTGTCGATCTTGCGG
TGCGCGCGACGCGGCTCGATCCCAAAAGCACGCCTTTCGCTCCGCTAGTCAATGCGGTTTTGCGCAATCTTGCGCGTGGG
CGGGAAAAAATCCTCGCCGAGAGTGACGCGCTGGCCATGGATACGCCGGCTTGGCTCGCCAAGCGCTGGCGCGAGACTTA
TGGCGAAGAACTCGCCCGCGCCATCGCTTTGGCGCATCGCGAAGAACCGACCCTCGATGTGACGGTCTTGGCCGAGCCGG
ATCTCTGGGCGGAAAAGCTTGGAGCCCGCCTGCTGCCGACGGGTTCATTGCGCCTTGAAACGCATGCGCCGATCGCCGAT
CTGCCCGGTTTCGCGGAAGGTGTGTGGTTCGTGCAAGATGCCGCCGCGGCCCTGCCGGCGCGCCTCATGCCGGCCGGTCC
CGGCTTGCGCATCGCCGATTTTTGCGCGGCCCCGGGCGGCAAGGCCGCGCAACTTGCCGCAACGGGCGCGCATGTGACGG
CGATCGACCGCTCAGCAACCCGATTGAAACGGTTGAGCGCCAATTTCGCGCGATTGCATCTCGAAGCCGAGGTACTGGCT
GCCGATGTCTTAACCTTCGAGGCCGAGCCTTTCGATGGCATTCTGCTCGATGCGCCCTGTTCGGCGACTGGCACTATTCG
CCGGCATCCCGACATTGCCTGGATCAAACAGCCGAAGGACATTCTCGCCCTCGCTTTGGCGCAGGCACGCATGCTGGATA
AAGCGGCAACCCTTTTGAAAGCCGGCGGAACACTTATTTATTGCACCTGTTCGCTTGAACCCGAGGAAGGCGAAGAGCGG
ATCGAGGCTTTTCTCCAAAATCATCCCGAATTTCGGCGGAGGCCCATCAGCCCGGGAGAAGTCGGGAACTTGAGCGAAAT
CATTAACCAAAACGGCGATTTACGGATTCTTCCGGCGCATTTTCAAGGCACCGAGGCGCGTTTTTCTGGCCTTGATGGCT
TCTTCGTCTCTCGTTTGGTTCGGTGTGGTTAA

Upstream 100 bases:

>100_bases
TAGGAAGGCCGCATCAGGAAGAAGGAGAATGCGGCCTTCGAAACTTCCGTTGGCGCAACAGTCGCGACGTGCATAAAATG
AAATGTGAGAGATAAAAAGC

Downstream 100 bases:

>100_bases
TAAAGCCGAGAGGTTTTTAGCTTTACATGGGAGGCGGCCTTTACGACGGGCCTCGAAACGCGGGGCAGGGCAGTGGACGT
CACCTGGACTCATGTTGGAG

Product: sun protein

Products: NA

Alternate protein names: 16S rRNA m5C967 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase rsmB [H]

Number of amino acids: Translated: 463; Mature: 462

Protein sequence:

>463_residues
MANSSIRPAASGRSKLRKPAASSTEEQPAGLGVRRTAAAILRAIVTKGQALDDFFAPESNPEILAGLEPRDKALARSIVT
VALRRLGTIRAALTGLLAQGLPRQAPDLEWILLVAGAQILFLDVPDHAAVDLAVRATRLDPKSTPFAPLVNAVLRNLARG
REKILAESDALAMDTPAWLAKRWRETYGEELARAIALAHREEPTLDVTVLAEPDLWAEKLGARLLPTGSLRLETHAPIAD
LPGFAEGVWFVQDAAAALPARLMPAGPGLRIADFCAAPGGKAAQLAATGAHVTAIDRSATRLKRLSANFARLHLEAEVLA
ADVLTFEAEPFDGILLDAPCSATGTIRRHPDIAWIKQPKDILALALAQARMLDKAATLLKAGGTLIYCTCSLEPEEGEER
IEAFLQNHPEFRRRPISPGEVGNLSEIINQNGDLRILPAHFQGTEARFSGLDGFFVSRLVRCG

Sequences:

>Translated_463_residues
MANSSIRPAASGRSKLRKPAASSTEEQPAGLGVRRTAAAILRAIVTKGQALDDFFAPESNPEILAGLEPRDKALARSIVT
VALRRLGTIRAALTGLLAQGLPRQAPDLEWILLVAGAQILFLDVPDHAAVDLAVRATRLDPKSTPFAPLVNAVLRNLARG
REKILAESDALAMDTPAWLAKRWRETYGEELARAIALAHREEPTLDVTVLAEPDLWAEKLGARLLPTGSLRLETHAPIAD
LPGFAEGVWFVQDAAAALPARLMPAGPGLRIADFCAAPGGKAAQLAATGAHVTAIDRSATRLKRLSANFARLHLEAEVLA
ADVLTFEAEPFDGILLDAPCSATGTIRRHPDIAWIKQPKDILALALAQARMLDKAATLLKAGGTLIYCTCSLEPEEGEER
IEAFLQNHPEFRRRPISPGEVGNLSEIINQNGDLRILPAHFQGTEARFSGLDGFFVSRLVRCG
>Mature_462_residues
ANSSIRPAASGRSKLRKPAASSTEEQPAGLGVRRTAAAILRAIVTKGQALDDFFAPESNPEILAGLEPRDKALARSIVTV
ALRRLGTIRAALTGLLAQGLPRQAPDLEWILLVAGAQILFLDVPDHAAVDLAVRATRLDPKSTPFAPLVNAVLRNLARGR
EKILAESDALAMDTPAWLAKRWRETYGEELARAIALAHREEPTLDVTVLAEPDLWAEKLGARLLPTGSLRLETHAPIADL
PGFAEGVWFVQDAAAALPARLMPAGPGLRIADFCAAPGGKAAQLAATGAHVTAIDRSATRLKRLSANFARLHLEAEVLAA
DVLTFEAEPFDGILLDAPCSATGTIRRHPDIAWIKQPKDILALALAQARMLDKAATLLKAGGTLIYCTCSLEPEEGEERI
EAFLQNHPEFRRRPISPGEVGNLSEIINQNGDLRILPAHFQGTEARFSGLDGFFVSRLVRCG

Specific function: Specifically methylates the cytosine at position 967 (m5C967) of 16S rRNA [H]

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family [H]

Homologues:

Organism=Homo sapiens, GI76150625, Length=182, Percent_Identity=36.8131868131868, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI76150623, Length=182, Percent_Identity=36.8131868131868, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI32698918, Length=188, Percent_Identity=30.3191489361702, Blast_Score=86, Evalue=8e-17,
Organism=Homo sapiens, GI270288818, Length=193, Percent_Identity=33.6787564766839, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI270288816, Length=193, Percent_Identity=33.6787564766839, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI8922322, Length=193, Percent_Identity=33.6787564766839, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI23199998, Length=193, Percent_Identity=33.6787564766839, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI2367212, Length=449, Percent_Identity=33.8530066815145, Blast_Score=204, Evalue=7e-54,
Organism=Escherichia coli, GI87081985, Length=273, Percent_Identity=30.7692307692308, Blast_Score=101, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17536757, Length=250, Percent_Identity=28, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI71998419, Length=217, Percent_Identity=29.0322580645161, Blast_Score=69, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI115534021, Length=199, Percent_Identity=29.6482412060301, Blast_Score=69, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6324268, Length=213, Percent_Identity=31.924882629108, Blast_Score=91, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6319447, Length=176, Percent_Identity=34.0909090909091, Blast_Score=77, Evalue=4e-15,
Organism=Drosophila melanogaster, GI22024126, Length=182, Percent_Identity=32.967032967033, Blast_Score=93, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24668781, Length=190, Percent_Identity=31.5789473684211, Blast_Score=82, Evalue=5e-16,
Organism=Drosophila melanogaster, GI21356513, Length=217, Percent_Identity=29.0322580645161, Blast_Score=82, Evalue=6e-16,
Organism=Drosophila melanogaster, GI21355201, Length=181, Percent_Identity=31.4917127071823, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI19920494, Length=171, Percent_Identity=30.4093567251462, Blast_Score=73, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001678
- InterPro:   IPR018314
- InterPro:   IPR006027
- InterPro:   IPR004573 [H]

Pfam domain/function: PF01189 Nol1_Nop2_Fmu; PF01029 NusB [H]

EC number: =2.1.1.176 [H]

Molecular weight: Translated: 49686; Mature: 49555

Theoretical pI: Translated: 7.23; Mature: 7.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANSSIRPAASGRSKLRKPAASSTEEQPAGLGVRRTAAAILRAIVTKGQALDDFFAPESN
CCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHCCCCCC
PEILAGLEPRDKALARSIVTVALRRLGTIRAALTGLLAQGLPRQAPDLEWILLVAGAQIL
CCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCEEE
FLDVPDHAAVDLAVRATRLDPKSTPFAPLVNAVLRNLARGREKILAESDALAMDTPAWLA
EEECCCCCCEEHEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHH
KRWRETYGEELARAIALAHREEPTLDVTVLAEPDLWAEKLGARLLPTGSLRLETHAPIAD
HHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHCCEECCCCCEEEECCCCHHH
LPGFAEGVWFVQDAAAALPARLMPAGPGLRIADFCAAPGGKAAQLAATGAHVTAIDRSAT
CCCCHHHHHHHHHHHHHCCHHHCCCCCCCCHHHHHCCCCCCCEEEEECCCEEEEECHHHH
RLKRLSANFARLHLEAEVLAADVLTFEAEPFDGILLDAPCSATGTIRRHPDIAWIKQPKD
HHHHHHCHHHHEEEHHHHHHHHHHEEECCCCCCEEEECCCCCCCCCCCCCCCHHHCCCHH
ILALALAQARMLDKAATLLKAGGTLIYCTCSLEPEEGEERIEAFLQNHPEFRRRPISPGE
HHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCHHHHHHHHHCCHHHHCCCCCCCC
VGNLSEIINQNGDLRILPAHFQGTEARFSGLDGFFVSRLVRCG
CCCHHHHHCCCCCEEEEECCCCCCHHHHCCCCHHHHHHHHHCC
>Mature Secondary Structure 
ANSSIRPAASGRSKLRKPAASSTEEQPAGLGVRRTAAAILRAIVTKGQALDDFFAPESN
CCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHCCCCCC
PEILAGLEPRDKALARSIVTVALRRLGTIRAALTGLLAQGLPRQAPDLEWILLVAGAQIL
CCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCEEE
FLDVPDHAAVDLAVRATRLDPKSTPFAPLVNAVLRNLARGREKILAESDALAMDTPAWLA
EEECCCCCCEEHEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHH
KRWRETYGEELARAIALAHREEPTLDVTVLAEPDLWAEKLGARLLPTGSLRLETHAPIAD
HHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHCCEECCCCCEEEECCCCHHH
LPGFAEGVWFVQDAAAALPARLMPAGPGLRIADFCAAPGGKAAQLAATGAHVTAIDRSAT
CCCCHHHHHHHHHHHHHCCHHHCCCCCCCCHHHHHCCCCCCCEEEEECCCEEEEECHHHH
RLKRLSANFARLHLEAEVLAADVLTFEAEPFDGILLDAPCSATGTIRRHPDIAWIKQPKD
HHHHHHCHHHHEEEHHHHHHHHHHEEECCCCCCEEEECCCCCCCCCCCCCCCHHHCCCHH
ILALALAQARMLDKAATLLKAGGTLIYCTCSLEPEEGEERIEAFLQNHPEFRRRPISPGE
HHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCHHHHHHHHHCCHHHHCCCCCCCC
VGNLSEIINQNGDLRILPAHFQGTEARFSGLDGFFVSRLVRCG
CCCHHHHHCCCCCEEEEECCCCCCHHHHCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]